DOI QR코드

DOI QR Code

Lactobacillus plantarum Improves the Nutritional Quality of Italian Ryegrass with Alfalfa Mediated Silage

  • Ilavenil, Soundarrajan (Grassland and Forage division, National Institute of Animal Science, RDA) ;
  • Arasu, Mariadhas Valan (Grassland and Forage division, National Institute of Animal Science, RDA) ;
  • Vijayakumar, Mayakrishnan (Grassland and Forage division, National Institute of Animal Science, RDA) ;
  • Jung, Min-Woong (Grassland and Forage division, National Institute of Animal Science, RDA) ;
  • Park, Hyung Soo (Grassland and Forage division, National Institute of Animal Science, RDA) ;
  • Lim, Young Cheol (Grassland and Forage division, National Institute of Animal Science, RDA) ;
  • Choi, Ki Choon (Grassland and Forage division, National Institute of Animal Science, RDA)
  • Received : 2014.08.13
  • Accepted : 2014.09.19
  • Published : 2014.09.30

Abstract

The present study was planned to analyze the nutritional quality, microbial counts and fermentative acids in Italian ryegrass (IRG) 80% and alfalfa 20% (IRG-HV) mediated silage inoculated with lactic acid bacteria (LAB) as a probiotic strain for 3 months. Crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), total digestible nutrient (TDN) and In-vitro dry matter digestibility (IVDMD), lactic acid bacteria (LAB), yeast and fungi counts and fermentation metabolites such as lactic acid, acetic acid and butyric acids were analyzed. The result shows that the nutritional quality and metabolite profiles of silage were significantly improved with LAB. For microbial counts, LAB showed dominant followed by yeast as compared with control silage. The pH of the silage also reduced significantly when silage inoculated with LAB. The result confirmed that silage preparation using different crops with L. plantarum inoculation is most beneficial for the farmers.

Keywords

References

  1. AOAC. 1990. Official methods of analysis (15th ed.). Association of analytical chemists, Washington, DC.
  2. Ashbell, G., Weinberg, Z.G., Hen, Y. and Filya, I. 2002. The effects of temperature on the aerobic stability of wheat and corn silages. Journal of Industrial Microbiology and Biotechnology. 28: 261-263. https://doi.org/10.1038/sj.jim.7000237
  3. Ávila, C.L.S., Valeriano, A.R., Pinto J.C., Fiqueiredo, H.C.P., Rezende, A.V. and Schwan, R.F. 2010. Chemical and microbiological characteristics of sugarcane silages treated with microbial inoculants. Revista Brasileira de Zootecnia. 39:25-32. https://doi.org/10.1590/S1516-35982010000100004
  4. Cooke, K.M., Bernard, J.K. and West J.W. 2009. Performance of lactating dairy cows fed ryegrass silage and corn silage with ground corn, steam flaked corn, or hominy feed. Journal of Dairy Science. 92:1117-1123. https://doi.org/10.3168/jds.2008-1481
  5. Danner, H., Holzer, M., Mayrhuber, E. and Braun, R. 2003. Acetic acid increases stability of silage under aerobic conditions. Applied and Environmental Microbiology. 69:562-567. https://doi.org/10.1128/AEM.69.1.562-567.2003
  6. Filya, I. 2003. The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. Journal of Dairy Science. 86:3575-3581. https://doi.org/10.3168/jds.S0022-0302(03)73963-0
  7. Giraffa, G., Chanishvili, N. and Widyastuti, Y. 2010. Importance of lactobacilli in food and feed biotechnology. Research in Microbiology. 161:480-487. https://doi.org/10.1016/j.resmic.2010.03.001
  8. Kristensen, N.B., Storm, A., Raun, B.M.L., Rojen, B.A. and Harmon, D.L. 2007. Metabolism of silage alcohols in lactating dairy cows. Journal of Dairy Science. 90:1364-1377. https://doi.org/10.3168/jds.S0022-0302(07)71623-5
  9. Lin, C., Bolsen, K.K., Brent, B.E., Hart, R.A., Dickersen, J.T., Feyerherm, A.M. and Aimutis, W.R. 1992. Epiphytic microflora on alfalfa and whole-plant corn. Journal of Dairy Science. 75:2484-2493. https://doi.org/10.3168/jds.S0022-0302(92)78010-2
  10. McDonald, P., Henderson, A.R. and Heron, S.J.E. 1991. The Biochemistry of silage. Chalcombe Publications, Marlow, Buckinghamshire, Bucks, UK.
  11. Miller, T.L. and Wolin, M.J. 1974. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Applied Microbiology. 27:985-987.
  12. Moore, J.E. 1970. Procedure for the two-stage in-vitro digestion of forage.In:p.5501. Nutritional research Techniques for Domestic and wild animals, vol.1L.E. Harris, Utah State University.
  13. Ojeda, F. and Montejo, I. 2001. Conservacion de morera (Morus alba) como ensilaje. I. Efecto sobre los compuestos nitrogenados. Pastos y Forrajes. 24:147-155.
  14. Van Soest, P.J. 1993. Cell wall matrix interactions and degradation session synopsis. In H.G. Jung, D.R. Buxton, R.D. Hatfield, and J. Ralph eds. Forage Cell Wall Structure and Digestibility 377-395, American Soc. Agronomy, Madison, WI.
  15. Weinberg, Z.G. and Muck, R.E. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiology Reviews. 19:53-68.

Cited by

  1. Effect of Addition of Chlorella and Lactic Acid Bacteria on Nutritive Value and Fermentation Quality of Fresh Rice Straw Silage vol.35, pp.2, 2015, https://doi.org/10.5333/KGFS.2015.35.2.159
  2. Effect of Novel Lactobacillus plantarum KCC-10 and KCC-19 on Fermentation Characterization of Alfalfa Silage vol.35, pp.2, 2015, https://doi.org/10.5333/KGFS.2015.35.2.166
  3. Addition of Novel Lactobacillus plantarum KCC-10 and KCC-19 to Improve Fermentation Quality and Characterization of Italian Ryegrass Silage vol.35, pp.3, 2015, https://doi.org/10.5333/KGFS.2015.35.3.195
  4. Effect of Lactic Acid Bacteria Inoculation on Fermentation Characteristics of Whole Crop Barley Silage vol.35, pp.3, 2015, https://doi.org/10.5333/KGFS.2015.35.3.201
  5. Beneficial Effects of Lactic Acid Bacteria Inoculation on Oat Based Silage in South Korea vol.35, pp.3, 2015, https://doi.org/10.5333/KGFS.2015.35.3.207
  6. Effect of Addition of Lactic Acid Bacteria on Fermentation Quality of Rye Silage vol.35, pp.4, 2015, https://doi.org/10.5333/KGFS.2015.35.4.277
  7. Potential effects of Novel Lactic Acid Bacteria on Fermentation Quality of Rye Haylage vol.36, pp.1, 2016, https://doi.org/10.5333/KGFS.2016.36.1.23
  8. Effects of Selected Inoculants on Chemical Compositions and Fermentation Indices of Rye Silage Harvested at Dough Stage vol.38, pp.2, 2018, https://doi.org/10.5333/KGFS.2018.38.2.99