• Asian-Australasian Journal of Animal Sciences
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• v.14 no.9
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• pp.1233-1237
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• 2001

A study was conducted to determine the effects of maturity at harvest and wilting days on the quality of round baled rye (Secale cereale L.) silage. This study was a $3{\times}3$ factorial arrangement in a split plot design with 3 replicates. The main plot was 3 harvesting dates at the stage of boot (20 Apr.), heading (29 Apr.) and flowering (14 May). The subplot was wilting day : 0 (unwilted), 0.5 and 1 day (0, 1, and 2 days at boot stage). Acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents of rye silage were significantly greater than those of rye before ensiling, but crude protein (CP) content and in vitro dry matter digestibility (IVDMD) were vice versa. Buffering capacity (BC) of rye harvested at flowering stage was decreased from 264 to 202 meq/kg at 1 day wilting, however, it was increased when harvested at boot or heading stage. The pH in wilted silage was the highest while that of flowering stage was the lowest. Water soluble carbohydrate (WSC) content of wilting rye was lower than that of unwilted, and the lowest at late harvesting stage. All plots had minimal WSC content for silage fermentation. Wilting treatment and delayed harvesting date caused an increase in dry matter (DM) content of round bale silage. The content of ammonia-N expressed as a portion of total N showed negative correlation with DM content. High quality silage according to ammonia-N content could be obtained from mid-harvest with wilting. There were highly significant differences in each organic acid between harvesting dates and wilting periods. Acetic and butyric acid contents were increased with delayed harvesting and prolonged wilting period, the lactic acid content, however, was decreased. This study demonstrated that harvest of rye from heading to flowering stage with wilting would be a recommendable method for making high quality rye silage using round bale system.

• Journal of Plant Biology
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• v.39 no.4
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• pp.257-263
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• 1996

Flower-inducing activity in the phloem exudata of Pharbitis cotyledons was investigated using the bioassay of Pharbitis and Lemna. By SDS-PAGE and 2-D gel electrophoresis of the phloem exudate, two polypeptides of 11 kDa and of approximately 32 kDa (pI 6.9) showing qualitative changes during the flower induction were detected. A polypeptide of approximately 20 kDa (pI 4.8) specifically labeled in vivo with [35S]methionine was found during the inductive dark period in Pharbitis cotyledon tissues. The polypeptide of the equivalent molecular mass and with the identicl pI value was also detected by in vitro translation assay. Thus, it is assumed that the 20 kDa polypeptide plays a role in the process of flower induction in Pharbitis cotyledons.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.1
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• pp.44-49
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• 2003

In vitro culture of panicle has been the method to accumulate starch and protein in immature grains by providing nutrients after florets crossed between remote genotypes artificially. Grain filling means embryo development and sucrose translocation from photosynthetic source, and starch manufacture in endosperm. The concentrations of sucrose used to culture immature rice panicle were 5, 10, 15, 20% and glutamine was 20 mM. When immature rice panicles at 5 days after flowering were cultured in distilled water with different concentrations of sucrose, glutamine 20 mM and MS medium with different concentrations of sucrose, glutamine 20 mM for 30 days the later was effective on grain filling. The optimal concentration of sucrose on grain filling in vitro culture of rice panicle was 10% and the weight of grain cultured was 10.14 mg that was corresponded to 57% of intact plant. In the method of treating plant growth regulators, the culture of immature rice panicle adding in MS medium with Kinetin, IAA, $\textrm{GA}_3$ were effective on grain filling than the culturing of immature rice panicle after submerging in solutions of Kinetin, IAA, $\textrm{GA}_3$ for 1day. When immature rice panicle was cultured in MS medium with sucrose 10% and Kinetin 46.47 $\mu$M it was effective on grain filling, respectively. The weight of grain cultured was 13.1mg that was corresponded to 75% of intact and germination rate was 51 %. When immature rice panicles were cultured in medium with different concentrations combined with Kinetin 4.65, 46.47, 464.7 $\mu\textrm{M}$, IAA 5.71, 57.08, 570.80 $\mu\textrm{M}$ for 30 days and in medium with IAA 5.71, 57.08, 570.80 $\mu\textrm{M}$ for 15 days after culturing in medium with Kinetin 4.65, 46.47, 464.70 $\mu\textrm{M}$ for 15 days the effect on grain filling was similar.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.2
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• pp.31-36
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• 1982

The field evaluation were conducted on dihaploid progenies derived from anther culture of F1 plant of Drama x Sohyang (N. tabacum L.) The mean values of agronomic and chemical traits of dihaploids such as Nicotine, length/width, plant height, Leaf length, leaves per plant and Yield were greater than those of tar parents. The correlation coefficient of dihaploids were similar to those of conventional lines and varieties. In path analysis, in relating yield components to yield, leaves per plant and leaf width had a direct effect on Yield, but the other components such as plant height, leaf length and days to flowering influenced Yield indirect way.

• Research in Plant Disease
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• v.10 no.4
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• pp.297-303
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• 2004

Bacterial blossom blight of kiwifruit (Actinidia deliciosa) has been mainly controlled by antibiotics. Among 15 candidate chemicals, streptomycin sulfate oxytetracycline WP, streptomycin copper hydroxide WP and oxolinic acid WP were selected as preventive bactericides against bacterial blossom blight of kiwfruit through in vitro and in vivo test. Spray of streptomycin sulfate oxytetracycline WP and streptomycin copper hydroxide WP at flowering period was most effective in controlling bacterial blossom blight of kiwifruit. Among the various combinations of spray times at different spray periods, optimum spray program of the preventive bactericides for the control of bacterial blossom blight of kiwifruit was turned out to be 3 times application with 10 day-interval from early May during the flowering season of kiwifruits.

• Korean Journal of Plant Tissue Culture
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• v.25 no.3
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• pp.195-200
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• 1998

Callus induction from leaf and stem explants of Arabidopsis thaliana was successfully obtained when leaf explants were cultured on MS medium containing 2.0 mg/L 2,4-D in the dark and also, when stem explants were cultured on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP. Explant-derived sliced calli were suspension-subcultured every week in CP liquid medium with 0.5 mg/L 2,4-D and 0.1 mg/L BAP in the dark, and shoot-forming cell clusters of nodular, pale yellow and knobby type were selected after 7-8 weeks of culture. Shoots were initiated from the green spots of the selected shoot forming calli cultured on MS regeneration medium containing 0.05 mg/L IAA, 7.0 mg/L 2-iP and 30 g/L sucrose under continous illumination for four weeks. Shoot regeneration frequency (calli regenerating at least one shoot) was more than 50%. For plant regeneration, excised shoots were trnasferred to hormone free medium for root initiation after 4 weeks of culture. The regenerants were bolting after 2 weeks of culture and formed in vitro flowering buds within bracts after 4 weeks of culture.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.2
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• pp.107-112
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• 2011

This experiment was carried out to provide some information on the change of dry matter productivity and feed value (forage quality) according to growth stages of late-maturing variety, Hwasan 101, Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forages Division, National Institute of Animal Science, RDA, Suwon from 2000 to 2002. In Suwon, booting, first heading, full heading and flowering stages of cv. Hwasan 101 were May 8, 18, 28 and June 3, respectively. Dry matter (DM) and total digestible nutrients (TDN) yields of Hwasan 101 were tendency to be increased by the progress of growth. DM yield in first heading stage increased 50% more than DM 4,818 kg/ha of booting stage, and also DM yield in full heading stage was increased by 26% of DM 7,244 kg/ha of first heading. Crude protein content was decreased gradually by the progress of growth, which were 18.32, 15.12, 12.58 and 12.36% in booting, first heading, full heading and flowing stage, respectively. Acid detergent fiber and neutral detergent fiber contents was increased and in vitro dry matter digestibility decreased gradually with progress of growth, but there was no difference between full heading and flowering stages. Considering DM yield and feed value of Italian ryegrass, the suitable cutting times seems between full heading and flowering stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.3
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• pp.379-385
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• 1983

Forage productivity of Job's tears (Coix lachryma-jobi L.) and adlay (C. lachryma-jobi var. mayuen (ROMAN.) STAPF) and a hybrid corn (Kwangog) and sorghum-sudangrass hybrid (Pioneer 988) was compared in the southern part of Korea where black streaked dwarf virus' (BSDV) is prevalent. Emergence of Job's tears and adlay was more delayed compared to a corn or sorghum-sudangrass hybrid under the drought field conditions. Early growth and regrowth of Job's tears and adlay were less than those of sorghum-sudangrass hybrid. Fresh weight, dry weight, and digestible dry matter of Job's tears and adlay cut once approximately 40 days after flowering were much higher than those of two cuts at every flowering stage. Dry weight of one-cut Job's tears and ad lay harvested 40 days after flowering was similar to the total dry weight of three-cut sorghum-sudangrass hybrid but higher than that of silage corn. Digestible dry matter production was greater in the order of sorghum-sudangrass, corn, one-cut and two-cut Job's tears and adlay. Corn was severely infected with BSDV, but digestible dry matter was relatively high due to its higher in vitro dry matter digestibility. Among the Job's tears and adlay varieties, a variety of Job's tears with globular fruit shape was best for forage production due to its taller plant height, thick stem, wider leaf blades, higher leaf blade/total dry weight ratio, and higher forage yield. However, hulled grain yield of ad lay varieties was higher than that of Job's tears due to a higher hulled/rough grain ratio although they had similar rough grain yields.

• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.136-142
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• 2013

Optimal condition for pollen germination was suggested as a basic research of flowering physiology in order to identify the characteristics of flowering and seed setting of rare and endangered Forsythia saxatilis Nakai. Pollen samples were collected during flowering time from the end of March to the beginning of April. First, a suitable germination temperature, medium sucrose concentration and germination time were determined for pollen germination and pollen tube elongation in vitro, and then an optimal pH of culture medium. Pollen germination and tube elongation were significantly different among the levels of germination temperature, sucrose concentration and germination time. Interactive effects were observed between germination temperature and time, germination temperature and sucrose concentration, germination time and sucrose concentration. Pollen germination was the highest at $10^{\circ}C$ and increased with the increase of sucrose concentration, whereas it had no relation with germination time. In addition, pollen germination and tube elongation did not increase at more than 15% of sucrose concentration and 24 hours later. Pollen germination was the highest at pH 5 (20.8%) and the lowest at pH 6 (3.8%). In conclusion, $10^{\circ}C$, 15% sucrose and pH 5 were proposed as the optimal condition for pollen germination 24 hours later of pollen culture.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.73-80
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• 2003

Useful Dianthus species were collected and selected from two native and seven foreign species distributed in Gangwon province. For in vitro breeding,. callus was induced from the explants of apical meristem, leaf, stem and the in vitro adventitious shoots on MS basal medium with 2.0 mg/L 2,4-D and 0.5 mg/L BA at 27$^{\circ}C$ under continuous light. After 3 weeks of culture, calli initiated the most highly from the leaf explants of D. chinensis Organogenic calli were able to be selected from the adventitious shoot-derived calli. For shoot regeneration, these organogenic calli were cultured on MS medium with the combination of 0.1 mg/L NAA＋1.0 mg/L BA under continuous light. Multiple shoots were proliferated with low frequency (about 30%) from those adventitious shootderived calli. Also, shoots initiated directly from the adventitious shoot explants without callus formation at high frequency of 52% when cultured on N6 medium containing 0.1 mg/L NAA and 1.0 mg/L BA in D. gratianopol. Multiple shoots and plantlets grew well and rooted on MS medium supplemented with 0.1 mg/L NAA. Regenerants with well-developed roots were transferred to 8-cm pots containing vermiculite at 85% relative humidity and 27$^{\circ}C$ These plantlets were acclimatized in artificial soil mixture and transferred to the greenhouse for flowering with normal phenotypes. M28 Mutant line was selected with white flowers from 0.03M EMS-treated organogenic calli derived from in vitro adventitious shoot explants of D. chinensis and set seeds.