• Bulletin of the Korean Chemical Society
• /
• 제34권2호
• /
• pp.482-488
• /
• 2013

Two piperazine-containing 3,4-dihyroquinazolines (BK10007S/8S) have been synthesized, based on our previous work on the synthesis and antitumoral activity of 3,4-dihyroquinazolines. After evaluating them for T-type calcium channel blocking effect and in vitro anti-cancer effect, they were profiled for acute and repeat dose toxicity (40 mg/kg, 2 weeks) to BALB/c mice. BK10007S/8S were further in vivo evaluated against human pancreatic MIA PaCa-2 carcinoma in $BALB/c^{nu/nu}$ nude mice, which exhibited 54 and 61% tumor growth inhibition through 57-day oral administration of 2 mg/kg of body weight, respectively.

• Journal of Pharmaceutical Investigation
• /
• 제37권6호
• /
• pp.359-364
• /
• 2007

Pine bark extract is well-known as a very powerful antioxidant, anti-inflammatory, and antibiotic material. French maritime pine bark extract ($Pycnogenol^{(R)}$) of Horphag Research has monopolized the world market over 30 years. Korean red pine bark extract ($Pinexol^{(R)}$) was first manufactured by the patent technology of NutraPharm in Korea in 2006. Feasibility of topical gel and patch formulations of Pinexol was systematically investigated by evaluating the skin absorption of catechin as a reference compound. In vitro hairless mouse skin absorption of catechin from gel formulation was higher than that from patches. However, significant amount of catechin was also deposited inside the skin from patch formulations, which were dependent on the types of pressure sensitive adhesives. Thus, it seems to be feasible to control the topical delivery of Pinexol by using both gel and patch formulations, and be necessary to conduct further systematic investigation.

• Biomolecules & Therapeutics
• /
• 제23권4호
• /
• pp.379-385
• /
• 2015

The eye irritation potential of drug candidates or pharmaceutical ingredients should be evaluated if there is a possibility of ocular exposure. Traditionally, the ocular irritation has been evaluated by the rabbit Draize test. However, rabbit eyes are more sensitive to irritants than human eyes, therefore substantial level of false positives are unavoidable. To resolve this species difference, several three-dimensional human corneal epithelial (HCE) models have been developed as alternative eye irritation test methods. Recently, we introduced a new HCE model, MCTT HCE$^{TM}$ which is reconstructed with non-transformed human corneal cells from limbal tissues. Here, we examined if MCTT HCE$^{TM}$ can be employed to evaluate eye irritation potential of solid substances. Through optimization of washing method and exposure time, treatment time was established as 10 min and washing procedure was set up as 4 times of washing with 10 mL of PBS and shaking in 30 mL of PBS in a beaker. With the established eye irritation test protocol, 11 solid substances (5 non-irritants, 6 irritants) were evaluated which demonstrated an excellent predictive capacity (100% accuracy, 100% specificity and 100% sensitivity). We also compared the performance of our test method with rabbit Draize test results and in vitro cytotoxicity test with 2D human corneal epithelial cell lines.

• Biomolecules & Therapeutics
• /
• 제22권6호
• /
• pp.563-569
• /
• 2014

Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (${\mu}CT$) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with ${\mu}CT$ and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

• Korean Journal of Organic Agriculture
• /
• 제19권spc호
• /
• pp.144-147
• /
• 2011

The main nutritional problem of sorghum${\times}$sudangrass hybrid (Sorghum bicolor${\times}$Sorghum sudanese) silage is low quality and palatability. This experiment was conducted to evaluate whether organic com grain and crushed rice addition of sorghum${\times}$sudangrass hybrid silage increases forage quality of the silage. The sorghum${\times}$sudangrass hybrid silages with added com grain and crushed rice were similar to conventional com silage in moisture content. However organic sorghum${\times}$sudangrass hybrid silage had low pH values. Silage added with gain and byproduct had higher crude ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents than control com silages, while its non-fiber carbohydrate (NFC) and total digestible nutrients (TDN) showed the opposite results. Lactic acid, butyric acid and lactic percentage of total organic acid (L/T) of sorghum${\times}$sudangrass hybrid silages were higher than those of com silage, but acetic acid was higher than control. In vitro dry matter digestibility (IVDMD) of com silage was higher than other sorghum silages. Feed intake of sorghum${\times}$sudangrass hybrid silage added with crushed rice was highest among silages. Therefore, these data indicate that crushed rice and com grain added sorghum${\times}$sudangrass hybrid silage could be recommended as the most effective treatment for increasing silage quality and palatability.

• Journal of Technologic Dentistry
• /
• 제43권3호
• /
• pp.77-83
• /
• 2021

Purpose: The purpose of this in vitro study was to measure and compare the thickness-dependent color dimensions of digital light processing (DLP) three-dimensional (3D) printer and conventional interim restorative resin. Methods: Specimens (N=60) were fabricated using either subtractive manufacturing (S group) or DLP 3D printing (D group) material. All milled and 3D-printed specimens were allocated into three different groups (n=10) according to different thicknesses as follows: 1.0, 1.5, and 2.0 mm. Color measurements in the CIELab coordinates were made using a spectrophotometer under room light conditions (1,003 lux). The color differences (𝚫E^*) between the specimen and control target data were calculated. Data were analyzed using the oneway analysis of variance (ANOVA). Post hoc comparisons were conducted using Tukey's honestly significant difference method (α=0.05 for all tests). Results: The 𝚫L^*, 𝚫a^*, 𝚫b^*, and 𝚫E^* values of interim restorative resin produced by DLP 3D printing were obtained in terms of the specimen's thickness increased compared with the increases by subtractive manufacturing. When the thickness was similar, the color difference between subtractive manufacturing and DLP 3D printing was ≥5.5, which is a value required by the dentist for remanufacturing. Conclusion: Color was influenced by the thickness of the interim restorative resin produced by DLP 3D printing.

• The Journal of Advanced Prosthodontics
• /
• 제10권6호
• /
• pp.422-429
• /
• 2018

PURPOSE. Accurate color matching of maxillofacial prostheses to skin is important for esthetics. A computerized color matching system specific to human skin has recently been developed. The purpose of this study was to evaluate the accuracy in color and translucency matching of the computerized color matching system across different skin colors. MATERIALS AND METHODS. The silicone was colored to simulate 28 different skin colors (n=5) to serve as "target skin colors". Using a spectrocolorometer (e-skin), color codes were determined for "replicate skin color" fabrication. CIELAB Delta-E between target skin color-replicate skin color pairs and translucency parameter were calculated. CIELAB Delta-E values were compared with one-way ANOVA and Tukey multiple-comparison. The agreement between $L^*$, $a^*$, $b^*$ and translucency parameter of target skin colors and replicate skin color were calculated by a two-way mixed average measures intraclass correlation coefficient. Translucency parameter of target skin color- replicate skin color pairs were compared with Paired t-test (${\alpha}=.05$). RESULTS. The mean CIELAB Delta-E value was 3.83 and significant differences were found among colors. The intraclass correlation coefficient showed excellent reliability for $L^*$, $a^*$, $b^*$ and good reliability for translucency parameter (P<.001). The mean translucency parameter of replicate skin colors was significantly higher than that of translucency parameter. CONCLUSION. The computerized color matching system specific to human skin was found to be reliable in terms of color and translucency between target skin colors and replicate skin color.

• The Journal of Advanced Prosthodontics
• /
• 제11권2호
• /
• pp.105-111
• /
• 2019

PURPOSE. Limited data is available regarding the differences for possible microleakage problems and fitting accuracy of zirconia versus titanium abutments with various connection designs. The purpose of this in vitro study was to investigate the effect of connection design and abutment material on the sealing capability and fitting accuracy of abutments. MATERIALS AND METHODS. A total of 42 abutments with different connection designs [internal conical (IC), internal tri-channel (IT), and external hexagonal (EH)] and abutment materials [titanium (Ti) and zirconia (Zr)] were evaluated. The inner parts of implants were inoculated with $0.7{\mu}L$ of polymicrobial culture (P. gingivalis, T. forsythia, T. denticola and F. nucleatum) and connected with their respective abutments under sterile conditions. The penetration of bacteria into the surrounding media was assessed by the visual evaluation of turbidity at each time point and the number of colony forming units (CFUs) was counted. The marginal gap at the implant- abutment interface (IAI) was measured by scanning electron microscope. The data sets were statistically analyzed using Kruskal-Wallis followed by Mann-Whitney U tests with the Bonferroni-Holm correction (${\alpha}=.05$). RESULTS. Statistically significant difference was found among the groups based on the results of leaked colonies (P<.05). The EH-Ti group characterized by an external hexagonal connection were less resistant to bacterial leakage than the groups EH-Zr, IT-Zr, IT-Ti, IC-Zr, and IC-Ti (P<.05). The marginal misfit (in ${\mu}m$) of the groups were in the range of 2.7-4.0 (IC-Zr), 1.8-5.3 (IC-Ti), 6.5-17.1 (IT-Zr), 5.4-12.0 (IT-Ti), 16.8-22.7 (EH-Zr), and 10.3-15.4 (EH-Ti). CONCLUSION. The sealing capability and marginal fit of abutments were affected by the type of abutment material and connection design.

• Journal of Veterinary Clinics
• /
• 제30권2호
• /
• pp.115-118
• /
• 2013

An 8-year-old spayed female domestic shorthair cat was presented with a chief complaint of chronic nasal discharge and dyspnea. Physical examination revealed pyohemorrhagic nasal discharge, inspiratory dyspnea and stertor, and an enlarged right mandibular lymph node. Abnormalities of blood works and serum chemistry included mildly increased hematocrit, and globulin concentration. Serologic tests for FeLV and FIV, and a panel of polymerase chain reaction tests for Chlamydophila felis, Feline Calicivirus, Herpesvirus, Bordetella, Mycoplasma felis, and H1N1 influenza was all negative. Only radiographic finding showed increasing soft tissue density in the right nasal cavity and computed tomography disclosed soft tissue/fluid opacification in the right nasal cavity, paranasal sinus, and pharyinx along with slight deviation to the right of the osseous nasal septum. Focal lysis of ventral nasal septum was also suspected in CT scan. Cytological evaluation of fine needle aspirate smears of the enlarged mandibular lymph nodes revealed numerous fungal yeasts having variably thick capsule both extracellularly and intracellularly with low numbers of macrophages. Some yeasts showed narrow based budding, which was a consistent finding with Cryptococcus organisms. Serum protein electrophoresis was a polyclonal consistent with chronic infection and serum was submitted for a fungal serology panel test. In serologic tests Cryptococcus antigen titer was 1 : 32,768. In vitro culture was unsuccessful. Treatment was initiated with administration of fluconazole, clindamycin, and tocopherol. Clinical signs resolved within 3 days after the initial treatment. The cat was discharged and scheduled for periodic evaluation and continued therapy, but was lost to follow-up thereafter.

• The Korean Journal of Pesticide Science
• /
• 제18권2호
• /
• pp.115-121
• /
• 2014

This study was performed to investigate the effect of conidial density, wetness period and temperature on conidial germination, appressoria formation and disease incidence. While there was not significantly correlated between conidial density and temperature, and conidial germination and appressoria formation, there was a significant correlation between those factors and disease incidence. The longer wetness period was, the higher the ratio of conidial germination, appressoria formation and the disease incidence was. The optimum conidial density, temperature and wetness period was $1{\times}10^6$ conidia $mL^{-1}$, $30^{\circ}C$ and 5 days, respectively. In case the wetness period was more than 5 days, the typical symptom was not found on pepper fruits because of the overgrowth of mycelia. Using this fruit assay method, which the pepper anthracnose pathogens were inoculated by spraying spore suspension on non-wounded or wounded pepper fruits, control effect of three fungicides were evaluated against pepper anthracnose by the protective and/or the curative application. Propineb showed high protective control activity, while it showed curative control activity on unwounded fruits, but did not showed curative control activity on wounded fruits. Tebuconazole, one of curative fungicide, showed higher control activity in non-wound inoculation than wound inoculation. Trifloxystrobin, one of strobilurin group, showed high both protective and control activity against anthracnose. In conclusion, we supposed that the newly developed in vitro pepper fruit assay can be used to evaluate antifungal activity of control agents against pepper anthracnose.