• Title/Summary/Keyword: in vitro digestibility

Search Result 544, Processing Time 0.025 seconds

Effects of Inoculants on the Quality of Round Baled Grass Silage (유산균제 첨가가 라운드베일 목초 사일리지의 품질에 미치는 영향)

  • Kim, Jong-Geun;Chung, Eui-Soo;Seo, Sung;Ham, Jun-Sang;Yoon, Sei-Hyung;Lim, Young-Chul
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.26 no.3
    • /
    • pp.139-146
    • /
    • 2006
  • This experiment was conducted to determine the effect of microbial inoculant on the quality of round baled grass silage at experimental field of Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consist of randomized block design with three replications. The treatments were three different inoculant (control, inoculant A, B and C). The contents of ether extract(EE), crude protein(CP) and in vitro dry matter digestibility(IVDMD) in inoculant treatment plots were higher than those in control. Silages treated by Inoculant A, Band C had significantly lower acidity than that of control silage (p<0.05). Dry matter(DM) content of control silage was higher than those of inoculant treated silage. There were significant differences in organic acid contents among treatments(p<0.05). Lactic acid was increased with inoculant treatment, but, acetic and butyric acid was decreased. The DM loss of all silages were decreased with inoculant treatment and quality grade of inoculant treated silage was higher than that of control. Results of this study indicate that addition of microbial inoculant will improve the fermentation and quality of round baled grass silage.

A Very Early-Maturing Italian Ryegrass (Lolium multiflorum Lam.) New Variety, 'Greenfarm3ho' (이탈리안 라이그라스 신품종 '그린팜 3호'의 생육특성과 수량성)

  • Ji, Hee Chung;Whang, Tae Young;Kim, Ki-Yong;Choe, Hyunsoak;Hong, Ki Hung;Choe, Kuh Wann;Lee, Ki-Won;Lee, Sang-Hoon
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.35 no.1
    • /
    • pp.31-35
    • /
    • 2015
  • This experiment was carried out to breed a very early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea from 2012 to 2014. New variety "Greenfarm3ho" is a diploid variety with a green leaf color, a semi-erect growth habit in late autumn/fall, and an erect growth habit in early spring. With a heading date of April 27, Greenfarm3ho was categorized as an early-maturing variety. Compared with the "Greenfarm" control variety, Greenfarm3ho's flag-leaf width was narrower by 0.5 mm, its flag-leaf length was longer by 4.4 cm, and its plant length was longer by 3 cm; additionally, Greenfarm3ho's stem thickness was 0.1 mm thicker and it showed winter hardiness. The dry matter (DM) yield (8,976 kg/ha) of Greenfarm3ho was similar to that of Greenfarm. The in vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN), and crude protein (CP) of Greenfarm3ho were 70.0%, 64.1%, and 1.0%, respectively, which are higher than those of Greenfarm by 2.9%, 2.0%, and 1.4%, respectively. The acid detergent fiber (ADF) and neutral detergent fiber (NDF) content of Greenfarm3ho were 31.4% and 53.3%, respectively, which are lower than those of Greenfarm by 2.5% and 3.0%, respectively.

Effect of Harvest Stage of Corn on Nutritive Values and Quality of Roll Baled Corn Silage Manufactured with Corn Grown in Paddy Land (논에서 생산된 옥수수의 수확시기가 곤포사일리지의 사료가치와 품질에 미치는 영향)

  • Choi, Ki-Choon;Jo, Nam-Chul;Jung, Min-Woong;Lee, Kyung-Dong;Kim, Jong-Geun;Lim, Young-Chul;Kim, Won-Ho;Oh, Yung-Keun;Choi, Jin-Hyuk;Kim, Cheon-Man;Jung, Du-Keun;Choi, Jong-Man
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.31 no.1
    • /
    • pp.65-74
    • /
    • 2011
  • This study was carried out to examine the effect of harvest stage of corn on nutritive values and quality of round baled corn silage manufactured with corn grown in paddy land of Department of Animal Resources Development, National Institute of Animal Science, RDA from 2009 to 2010. Corn "Kwangpyungok" was harvested at three different growth times (milk, yellow ripen and ripen stage) and ensiled at each harvest stages. Crude protein (CP) and TDN (total digestible nutrient) contents of round baled corn silage was decreased and in vitro dry matter digestibility (IVDMD) was not changed with delayed harvest maturity. However, contents of ADF (acid detergent fiber) and NDF(neutral detergent fiber) decreased with delayed harvest maturity. The pH at three different harvest stages ranged from 3.8 to 4.0. The content of lactic acid increased with delayed harvest maturity, but the content of acetic acid decreased. And then, flieg's score reveals that there is an increase in order, ripen stage > yellow ripen stage > milk stage. Therefore, this study suggest that round baled corn silage manufactured at yellow ripen stage can improve the silage quality.

Comparative Tield and Quality of Summe Annual Grasses as Fresh-cut Forage (하형 청예 사료작물의 생산성과 사료가치 비교)

  • Jo, M.H.
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.6 no.3
    • /
    • pp.169-173
    • /
    • 1986
  • This experiment was conducted to provide comparative data on the newly imported thirteen sorghum-sudangrass hybrids(Sorghum bicolor(L.) Moench), TE Goldmaker sorghum hybrid (S. Bicolor (L.) Moench), NC+ 88SS sudangrass hybrid (S, bicolor (L.) Moench), Supermill pearlmillet (Pennisetum americanum (L.) Leeke) and teosinte (Euchlaena mexicana Schrad,) in comparison with the check variety Pioneer 988 sorghum-sudangrass hybrid for dry matter yield, protein content, in vitro dry matter digestibility(IVDMD), and other desirable agronomic characteristics, SX-17 and NC+855 gave significantly higher dry matter yield than the check variety. There was no significant difference in total dry matter yield of the remaining 14 varieties with the exception of teosinte and TE Goldmaker which had the lowest yield. The most varieties were moderately resistant to leaf diseases although differences exist among varieties. No diseases were found in pearlmillet and teosinte, but NC+ 88SS was very susceptible to leaf blight. The mean percent crude protein of sorghum-sudangrass hybrids was lower than that of pearlmillet and sorghum hybrid, but was higher than that of sudangrass hybrid, The IVDMD of G-83F, TE Goldmaker, teosinte and HW 5111 was higher than that of the check variety. There was no consistent relationship between the percent of crude protein and IVDMD of the summer annual grasses.

  • PDF

Characteristics of Tenebrio molitor larvae and Bombyx mori pupae sequentially fermented with Aspergillus oryzae and Bacillus subtilis (Aspergillus oryzae와 Bacillus subtilis를 순차접종 발효한 갈색거저리유충과 누에번데기 발효물의 품질특성)

  • Kang, Sanghun;Chung, Chang-Ho
    • Korean Journal of Food Science and Technology
    • /
    • v.54 no.1
    • /
    • pp.94-102
    • /
    • 2022
  • Defatted soybean, larvae of brown mealworm (Tenebrio molitor), and powdered pupae of silkworm (Bombyx mori) were fermented in solid and liquid forms using Aspergillus oryzae and Bacillus subtilis. The protein degradation rate (NDR) through solid fermentation was the highest in the fermented soybean control sample (54.69±6.54%), followed by silkworm pupae (34.82±5.99%) and brown mealworm larvae (30.54±3.80%). When these edible insects were fermented in liquid form, solid extraction yield was 37.73-46.88%, and protein yield was 47.47-63.02%. NDR of fermented liquid form products increased to 58.90, 52.62, and 50.13% for soybean, brown mealworm larvae, and silkworm pupae, respectively. SDS-PAGE of the liquid fermented products confirmed that microbial fermentation decomposed higher-molecular-weight proteins into small polypeptides. In vitro digestibility of liquid forms of edible insects increased by 1.26 to 1.53 times after fermentation. The protein solubility, foaming ability, and foam stability of liquid-fermented edible insects all tended to increase through fermentation.

Evaluation of Characteristics, Winter Survival and Forage Production for Warm Season Grass in the Mid-Southern Regions of Korea (중남부지역에서 난지형 목초의 생육특성, 월동성 및 사초생산성 평가)

  • Park, Hyung Soo;Jung, Min Woong;Jung, Yong Bok;Lim, Young Chul;Choi, Ki Choon;Kim, Ji Hye;Lee, Ki Won;Choi, Gi Jun
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.34 no.1
    • /
    • pp.1-8
    • /
    • 2014
  • Field studies were conducted from the years 2009 to 2012 in order to determine the cultivation limit as well as to evaluate the characteristics and forage production of warm season grass in Korea. Two bermudagrass [Cynodon dactylon (L.) Pers.] cultivars, two bahiagrass (Paspalum notatum Flugge) cultivars and a Kleingrass [Panicum coloratum L.] cultivar were compared for forage production and quality in the mid-southern regions of Korea. The experimental design was a randomized block design (RBD) with three replications. The number of days to seedling emergence for bremudagrass and bahiagrass was observed as approximately 12 days and 28 days after seeding, respectively. In Kwangju, the heading dates of bahiagrass and kleingrass were 21 August and 10 July, respectively,. Warm season grass did not winter in the mid-regions (Kimjea, Cheonan) of Korea. All of the Bermudagrass cultivars had higher dry matter (DM) than bahiagrass at the first harvest. The dry matter yield of kleingrass was usually greater than the other entries at all study sites. Peak forage DM production of bermudagrass and bahiagrass cultivars occurred in June and July, respectively. The contents of crude protein (CP) and total digestibility nutrient (TDN) for bermudagrass cultivars were usually greater than the other entries at all study sites. Further, acid detergent fiber (ADF) and in vitro DM digestibility (IVDMD) were similar across all cultivars.

Feed Value of Pearl Millet [Pennisetum americanum(L.) Leeke] Grown as a New Forage Crop (진주조의 사료적 가치)

  • Park, Keun-Yong;Choi, Byung-Han;Kang, Young-Kil;Moon, Hyeon-Gui;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.33 no.1
    • /
    • pp.48-53
    • /
    • 1988
  • Pearl millet has been detected as a promising new forage crop of excellent quality and productivity since 1985. Its green fodder yields were 10.7 to 12.8 tons per hectare in average of 26 accessions in Suwon, 1985. The yield level was much higher than those of Italian and pro so millets and com. Com was better than Italian and proso millets, and proso millet was better than Italian millet for a green fodder crop. Suwon 1 pearl millet hybrid was the best of the 13 hybrids examined in Suwon, 1986 being 149 tons per hectare of three times cut green fodder yields. The pearl millet hybrid was higher in green fodder yield than com and sorghum/sudan grass hybrids. Leaf area index was 32.4 for the three times cut pearl millet, while 5.8 for the one time cut corn, and 20.8 for the three times cut sorghum/sudan grass. Crude protein content was 16.3 percent for pearl millet being six to five percent higher than corn and sorghum/sudan grass, 11.8 percent for the one time cut at maturity and 16.1 percent for four times cut being higher than corn and sorghum/sudan grass. Crude fat content was 3. percent for pear 1 millet grain being some what higher than corn and sorghum/sudan grass and 1.3-1.4 percent for green fodder crop. Crude fiber content in grain was 1.9 percent for pearl millet 2.6 percent for corn, and 4.3 percent for sorghum/sudan grass. Crude fiber content in pearl millet plant was 24.4 to 26.8 percent, Crude ash content was 2.4 percent in grain and 10.8 to 11.6 percent in the plants of pearl millet hybrid. In vitro digestibility of grain was 93.7 percent for pearl millet, 95.4 percent for corn, and 55.8 percent for sorghum/sudan grass. The digestibility of whole plant was 57.6 to 63.4 percent for pearl millet, 46.3 percent for corn, and 47.3 to 57.6 percent for sorghum/sudan grass. Heavier nitrogen fertilizer applications increased green fodder yields, protein content and digestibility, but reduced fat and ash content of pearl millet inbred line T 186.

  • PDF

Spray-dried powder preparation of pumpkin sweet potato hydrolysates and its physicochemical properties (호박고구마 효소 분해물의 분무건조 분말 제조 및 물리화학적 품질특성)

  • Lee, Dae-Hoon;Jang, Jong-Hyun;Hong, Joo-Heon
    • Food Science and Preservation
    • /
    • v.24 no.2
    • /
    • pp.246-253
    • /
    • 2017
  • This study was conducted prepare spray-dried powder using pumpkin sweet potato hydrolysates and examine the physicochemical properties of the powder. The insoluble dietary fiber and soluble dietary fiber of the pumpkin sweet potato treated by enzyme were 4.17% and 2.07%, respectively. The spray-dried pumpkin sweet potato hydrolysates was manufactured via spray-drying with different forming agents: i.e., pectin 0.1%, 0.5%, 1%, and 2.0%. The moisture contents and total starches of the spray-dried powders were approximately 1.68-2.46 and 45.32-46.51%, respectively. The color of the L and a value decreased, and that of the b and ${\Delta}E$ value increased. The particle size and outer topology of the spray-dried powders were $37.17-42.32{\mu}m$, and its shape was generally globular. The water absorption index of the spray-dried powder (1.74-1.91) was lower than that of the freeze-dried powder (2.15). The water solubility index of the spray-dried powder, 80.75-87.61%, was higher than that of the freeze-dried powder (70.47%). The adhesion values of spray-dried powder to epithelial HT-29 cells were 2.66-6.18% of the initial cell counts, whereas freeze-dried powder showed lower adhesive ability (1.79%). The in vitro human digestibility in the spray-dried powder was 70.09% which is very effective in digestion.

Effect of Byproducts Supplementation by Partically Replacing Soybean Meal to a Total Mixed Ration on Rumen Fermentation Characteristics In Vitro (대두박 대체 부산물 위주의 TMR 사료가 반추위 내 미생물의 In Vitro 발효특성에 미치는 영향)

  • Bae, Gui Seck;Kim, Eun Joong;Song, Tae Ho;Song, Tae Hwa;Park, Tae Il;Choi, Nag Jin;Kwon, Chan Ho;Chang, Moon Baek
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.34 no.2
    • /
    • pp.129-140
    • /
    • 2014
  • This study was performed to evaluate the effects of replacing basic total mixed ration (TMR) with fermented soybean curd, Artemisia princeps Pampanini cv. Sajabal, and spent coffee grounds by-product on rumen microbial fermentation in vitro. Soybean in the basic TMR diet (control) was replaced by the following 9 treatments (3 replicates): maximum amounts of soybean curd (SC); fermented SC (FSC); 3, 5, and 10% FSC + fermented A. princeps Pampanini cv. Sajabal (1:1, DM basis, FSCS); and 3, 5, 10% FSC + fermented coffee meal (1:1, DM basis, FSCC) of soybean. FSC, FSCS, and FSCC were fermented using Lactobacillus acidophilus ATCC 496, Lactobacillus fermentum ATCC 1493, Lactobacillus plantarum KCTC 1048, and Lactobacillus casei IFO 3533. Replacing dairy cow TMR with FSC treatment led to a pH value of 6 after 8 h of incubation-the lowest value measured (p<0.05), and FSCS and FSCC treatments were higher than SC and FSC treatment after 6 h (p<0.05). Gas production was higher in response to 3% FSC and FSCC treatments than the control after 4-10 h. Dry matter digestibility was increased 0-12 h after FSC treatment (p<0.05) and was the highest after 24 h of 10% FSCS treatment. $NH_3-N$ concentration was the lowest after 24 h of FSC treatment (p<0.05). Microbial protein content increased in response to treatments that had been fermented by the Lactobacillus spp. compared to control and SC treatments (p<0.05). The total concentration of volatile fatty acids (VFAs) was increased after 6-12 h of FSC treatment (p<0.05), while the highest acetate proportion was observed 24 h after 5% and 10% FSCS treatments. The FSC of propionate proportion was increased for 0-10 h compared with among treatments (p<0.05). The highest acetate in the propionate ration was observed after 12 h of SC treatment and the lowest with FSCS 3% treatment after 24 h. Methane ($CH_4$) emulsion was lower with A. princeps Pampanini cv. Sajabal and spent coffee grounds treatments than with the control, SC, and FSC treatments. These experiments were designed to replace the by-products of dairy cow TMR with SC, FSC, FSCS, and FSCC to improve TMR quality. Condensed tannins contained in FSCS and FSCC treatments, which reduced $CH_4$ emulsion in vitro, decreased rumen microbial fermentation during the early incubation time. Therefore, future experiments are required to develop a rumen continuous culture system and an in vivo test to optimize the percentages of FSC, FSCS, and FSCC in the TMR diet of the dairy cows.

Effect of Pepsin-HC$\ell$ Concentration and Digestion Time on the Protein Digestibility of the Cattle Skin Meal (Pepsin농도와 소화시간이 우피분의 단백질 소화율에 미치는 영향)

  • 김대진
    • Korean Journal of Poultry Science
    • /
    • v.11 no.1
    • /
    • pp.13-17
    • /
    • 1984
  • Protein digestibitlities of hydrolyzed cattle skin meals were examined at a constant pepsin-MC$\ell$ concentration (0.2%) for varying lengths of incubation time (from 4 to 20 hours) and at varying concentrations of pepsin-HC$\ell$ (from 0.0125 to 0.2%) for 16 hours at 45$^{\circ}C$. The results are summarized as follows: 1. Protein digestibilities of hydrolyzed cattle skin meals in 0.2% pepsin-HCl were 66.31%, 80.69%, 83.72%, 84.65% and 81.45% for 4, 8, 12, 16 and 20 hours incubation, respectively. Protein digestibilities were maintained above 80% for 8-hour incubation and were increasing incubation time. 2. Protein digestibilities of hydrolyzed cattle skin meal incubated for 16 hours at 0.2%, 0.1%, 0.05%, 0.025% and 0.0125% pepsin-HC$\ell$ solution were 85.10%, 82.08%, 76.18%, 74.67% and 64.82%, respectively. Protein digestibilities were decreased with decreasing pepsin concentration.

  • PDF