• International Journal of Industrial Entomology and Biomaterials
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• 제15권2호
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• pp.101-106
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• 2007

During mulberry cultivation, root disease complex caused by the association of root knot nematode (Meloidogyne incognita) with root rot pathogens like Fusarium solani and Botryodiplodia theobromae poses serious loss in leaf production. Therefore, an attempt was made to assess the efficacy of eight pesticides (Metayalaxyl+Mancozeb, Thiophanate methyl, Mancozeb, Bitertanol, Phenomiphos, Phorate, Thionazin & Carbofuran) and two growth hormones (Salicylic acid and Indole 3 acetic acid) at 0.1 and 0.2% concentrations under in vitro conditions against nematode (hatching of eggs and mortality of larvae) and root rot pathogens (poisoned food technique) for short listing the treatments to develop an IDM strategy. Results revealed that among the pesticides and growth hormones, Carbofuran followed by Salicylic acid were found to be effective at 0.2% concentration against both nematode and pathogenic fungi. Both the chemicals inhibited the hatching of nematode eggs by 83.5-78.9% and 80-76% larval mortality over the control and reduced the mycelial growth of both the pathogenic fungi to an extent of 75.5-77.8%. Though Mancozeb inhibited both the pathogenic fungi strongly (77-80%), it did not show any effectiveness against nematode. The rest of the chemicals were found either moderately or poorly effective in reducing the growth of pathogenic fungi, hatching of nematode eggs and enhancing the mortality of larvae. The two effective chemicals viz., Carbofuran and Salicylic acid, which rated as strong inhibitors against both nematode and pathogenic fungi, can be exploited in developing an IDM package as one of the component for better management of root disease complex in mulberry.

• 한국유기농업학회지
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• 제20권2호
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• pp.201-209
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• 2012

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다.

• 원예과학기술지
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• 제33권1호
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• pp.106-113
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• 2015

Effects of mineral salts (N, P, K, $Ca^{2+}$, $Mg^{2+}$, and $Fe^{3+}$) on the shoot growth and metabolite production of tea tree were studied using in vitro culture techniques. Among mineral s alts, ${H_2PO_4}^-$ was the most important for enhanced growth rate of tea tree, while $Mg^{2+}$ and $Ca^{2+}$ did not affect plant growth. Removal of ${NH_4}^+$ and $NO_3$ from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of $Ca^{2+}$ decreased the production of caffeine; however, other treatments did not influence its production. $Ca^{2+}$, ${NH_4}^+$ and $Fe^{3+}$ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future.

• Mycobiology
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• 제32권1호
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• pp.1-5
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• 2004

The present study was carried out to investigate morphological characteristics of pseudosclerotia of Grifola umbellata formed by artificial cultures. Isolate G. umbellata DUM GUS-01 was obtained from sclerotium cultivated in field. The fungal isolate was cultured on PDYM broth, PDYMA(potato dextrose yeast malt agar) and oak sawdust media at $20^{\circ}C$ under the dark condition. G. umbellata DUM GUS-01 showed a volumetric increment of fungal lumps rather than mycelial growth. Particularly, G. umbellata DUM GUS-01 produced a large amount of melanin pigments in all culture treatments. The color of the fungal mass has been changed into grey gradually, and then formed melanized rind-like structure on its superficial part. The fungal structures which were covered with melanized rind-like layer were named as pseudosclerotia of G. umbellata. The pseudosclerotia of G. umbellata DUM GUS-01 formed a new white mycelial mass, which was swollen out of the melanized rind structure for its volumetric increment. When the pseudosclerotia were sectioned, their structure was discriminated from two structures such as a melanized rind-like structure layer formed by aggregation of aged mycelia and a white mycelial mass with high density. As results of scanning electron microscopic examination, the pseudosclerotia of G. umbellata DUM GUS-01 which were formed in in vitro conditions were similar to the sclerotia of G. umbellata cultivated in natural conditions except for the crystals formed in medula layer of natural sclerotia. Although size, solidity of rind structure and mycelial compactness of pseudosclerotia were more poor than those of natural sclerotia, the morphological structure and growth pattern of pseudosclerotia were very similar to those of natural sclerotia. Therefore, it is probable to induce pseudosclerotia to sclerotia of G. umbellata in in vitro conditions. Consequently, it seems that the induced pseudosclerotia can be used as inoculum sources to substitute natural sclerotia in field cultivation.

• 한국초지조사료학회지
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• 제20권4호
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• pp.237-242
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• 2000

This study was conducted to investigate the effects of cutting frequency on dry matter yield and nutritive value between heading and headless varieties of sorghumxsudangrass hybrid in 1998. Treatments were var. TE hay grazer(heading type) and Jumbo(headless type) as main plot, and 1, 2, and 3 cutting times per a year as sub plot. Although plant height of sorghumxsudangrass hybrid was no significant difference among treatments, headless type was very slightly higher than that of heading type, and both of variety decreased with 1, 2, and 3 cutting times in order. Also, dry matter yield of sorghum~sudangrass hybrid was no significant difference between heading and headless type. Dry matter yield of first cutting plot in heading type and in headless type was the higher than those of second and third cutting plot. Acid detergent fiber and neutral detergent fiber of headless type were slightly higher than that of heading type. Acid detergent fiber of sorghumxsudangrass hybrid was highest at first cutting plot in both varieties. Neutral detergent fiber of second cutting plot in heading type and first cutting plot in headless type were the highest respectively. In vitro dry matter digestibility of headless type was very slightly higher than that of heading type. And in vitro dry matter digestibility of second cutting plot was the highest in both varieties. In vitro digestible dry matter of sorghumxsudangrass hybrid was no significant difference among treatments. The results demonstrated that although there was not significant difference, cultivation of headless type and first cutting plot per a year were very good for nutritive value and DM yield of sorghumxsudangrass hybrid as summer forage crops in Korea. (Key words : Heading, Headless type, Cutting frequency, Sorghum X Sudangrass Hybrid)

• 한국자원식물학회지
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• 제21권5호
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• pp.347-351
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• 2008

뿌리에 진통, 진정, 건위, 구충 효능이 있다고 알려진 석창포(Acorus gramineus Soland)의 확대재배를 위하여 현재 분근증식만으로 한정되어 있는 번식법을 개선하고자 종자번식과 기내배양에 의한 번식 방법을 검토하였다. 종자번식을 위한 수확적기는 종피가 연녹색을 띠었을 때가 적합하였으며, 종자의 보관은 $5^{\circ}C$에서 30일까지 저장하는 것이 발아율을 높게 유지할 수 있었다. 석창포의 기내발아에는 $2.0mg{\cdot}L^{-1}$ NAA와 $0.1mg{\cdot}L^{-1}$ BA를 MS 기본배지에 첨가하여 다수의 신초를 유도한 후, $0.1mg{\cdot}L^{-1}$ BA 단독 처리 및 $0.5mg{\cdot}L^{-1}$ NAA와 $1.0mg{\cdot}L^{-1}$ BA 혼합처리에서 발근을 유도하는 것이 효과적이었다. 석창포 육묘상토로는 vermiculite보다 원예상토가 생육이 우수하였다.

• Plant Resources
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• 제6권3호
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• pp.205-210
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• 2003

Ginseng(Panax ginseng C.A. Meyer) is a perennial herbaceous plant which grows very slowly. It takes about 3 to 4 years from seeding to collecting the ripe seeds and the ginseng propagation is very difficult. and so, it is very difficult to breed ginseng plant. Ginseng tissue culture was started from at 1960, and ginseng commercial product by in vitro callus culture was saled, however upto now, regenerants were not planted to soil normally. Recently, plant genetic engineering to produce transgenic plants by introducing useful genes has been advanced greatly. In a present paper, transformation of ginseng plants was achieved by co-cultivation with Agrobacterium harboring the binary vector coding Proteinase-II gene, which confer resistant or tolerant to insect pests, The binary vector for transformation was constructed with disarmed Ti-plasmid and with double 35S promoter. The NPT II gene and introduced genes of the transgenic ginseng plants were successfully identified by the PCR. Especially the transgenic ginseng plants were regenerated using new techniques such as repetitive single somatic embryogenesis.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.364-372
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• 2016

Sand tubules, made up of sand grains cemented by microbe-induced calcium carbonate precipitation, have been found in China's Ningxia Province. Sand tubules grow like a tree's roots about 40-60 cm below the surface. The properties of sand tubules and their bacterial community were examined. X-Ray diffraction analysis revealed that the sand tubules were associated with crystalline calcite. Scanning electron microscopy showed that the crystalline solid had a lamellar structure and lacked the presence of cells, suggesting that no bacteria acted as nucleation sites, nor that the crystalline solid was formed by the aggregation of bacteria. Denaturing gradient gel electrophoresis analysis showed 11 of the 12 detectable bands were uncultured bacteria by BLAST analysis in the GenBank database, and the rest were closely related to Paenibacillus sp. (100% identity). By cultivation techniques, the only strain isolated from the sand tubule was suggested to be related to Paenibacillus sp.; no archaea were found. Furthermore, Paenibacillus sp. was demonstrated to induce calcium carbonate precipitation in vitro.

• 한국자원식물학회지
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• 제22권4호
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• pp.281-286
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• 2009

홍경천의 잎, 줄기, 뿌리 절편을 이용하여 기내 부정근의 생산 체계를 확립하였다. 먼저 홍경천의 잎, 줄기, 뿌리 절편을 0.1, 1.0, 3.0 및 5,0 mg/L의 IBA와 sucrose가 10, 30, 50 및 100 g/L가 첨가된 MS 배지위에 치상하여 부정근의 유도율을 조사하였다. 부정근의 유도는 잎, 줄기 절편에서 IBA의 농도가 5.0 mg/L 일때 가장 높은 유도율을 보였으며, 뿌리 절편은 IBA 3.0 mg/L 첨가된 배지에서 부정근 유도율이 가장 높았다. Sucrose의 농도는 30 g/L가 첨가되었을 때 잎, 줄기, 뿌리 절편에서 높은 유도율을 나타났다. 고체배지 조건에서 부정근의 유도율이 가장 우수한 조건을 기본으로 액체배양을 실시하였으며, 염의 농도에 따른 부정근의 증식조건을 조사하였다. 1/3MS 배지에서 홍경천의 부정근을 배양하였을 때 1/2MS, MS 액체 배지조건 보다 약 2배, 2.5배의 부정근 생장량을 보였다.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.539-556
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• 1995

Periodontal therapeutic modalities should be re-establishing and regenerating the periodontal tissue previously lost to the disease. To achieve periodontal regeneration, periodontal ligament cells must selective migrate to the deneded root surface, attached and proliferated it. Local pH concentration is one of the most factors that periodontal regeneration. The aims of this study were to examine on biological effects of pH to the human periodontal ligament cells in vitro, especially on the cell morphology, attachment, activity, vitality and viability. Human periodontal ligament cells were cultured from extracted tooth for non-periodontal reason. Immediately after extraction, any soft tissue adhering to the cervical parts of the roots was carefully removed with a sterile curette. To produce different pH levels in the media, Eagle's MEM was adjusted from pH 6.6 to 8.2 in 0.2 intervals with 1 M NaOH and 1 N HCl. After cultivation, Then, Periodontal ligament cells were cultured at pH ranging from 6.6-8.2. attachment assay was done at 1, 2 day incubation and activity assay was done at 1, 2, 3 day incubation. The experiments were evaluated by scaning electron microscopic techniques (HITACHIX-650 Scaning Electron Microanalyzer, Tokyo, Japan), MTT assay, and the cultured periodontal ligament cells were fixed in neutral formalin for 24 hours and immunohistochemically processed by PCNA for proliferating ability. The surviving cells in the medium showed slightly increased volume and widening intercellular distances at low concentration of pH than control group (pH 7.4), and apparently shrinkage at high concentration of pH than control group (pH 7.4). The results of the statistical analysis from the experiment on attachment, vitality and viability were as follows. Attachment of periodontal ligament cells at 1st and 2nd day, similar attachment rate of low concentration pH compared with control value (pH 7.4). But above pH 8.0, attachment rate were statistically significant decrease from control value(P<0.05). Periodontal ligament cell's activities were maximum at pH 7.6 by MTT assay. Similar with control value at low concentration of pH. But, the activities were statistically significant decrease at high concentraration of pH(P<0.05). Cellular proliferating rate (PCNA index) were statistically significant decrease from control value at low and high concentration of pH(p<0.05). This results suggested that hjgh concentration pH, in other words, alkali pH was cytotoxic effects on human periodontal ligament cells in vitro.