• Journal of the Korean Geosynthetics Society
• /
• v.11 no.1
• /
• pp.47-56
• /
• 2012

Discharge capacity of PBD is sensitive in proportion to thickness and ground condition, and drainage of PBD declines due to disturbance effect in surrounding ground by mandrel used for vertical drainage setting and setting machines and type. Also, deviation of discharge capacity gets larger according to ground condition, construction condition and soil properties. But cause and analysis of those problems like reduced discharge of capacity and delayed dissipation of pore water pressure for discharge capacity is lack. Thus, in this text, ground improvement and discharge capacity is investigated by implementing composite discharge capacity test for analysis of an effect factor of PBD discharge capacity with in-situ ground condition. After fixing the vertical drain on a cylindrical cylinder, put churned sample into the cylinder. After in-situ ground and reclamation of ground are dredged, load following the loading step of 30, 70 and 120kPa using a pressure device. Result of the test, The discharge capacity was SM>ML>CL>CL(dredged soil) in situ condition and more fine-grained content, the amount of discharge was greater.

• Korean Journal of Veterinary Research
• /
• v.39 no.3
• /
• pp.583-592
• /
• 1999

We have developed the in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues or cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies were observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.

• Tunnel and Underground Space
• /
• v.17 no.2 s.67
• /
• pp.90-101
• /
• 2007

With increasing development of underground space, underground pumped storage power plants, which generate power by felling water in upper reservoir to lower reservoir, have been continuously constructed. For efficient and safe design, construction and maintenance or such power plants, it is very important to understand in-situ stress and the mechanical properties of the surrounding rock mass at the design stage. The power plant presented in this paper is under construction at a depth of $320{\sim}375m$. For stability evaluation of the structure, in-situ stress was measured by over-coring method. Also pressurementer test and a series or laboratory tests were performed to obtain the mechanical properties. Numerical analyses were conducted to check the efficiency of designed support patterns. The results showed that unstable areas occurred partly in the numerical model, and therefore supports were additionally applied. Finally complete stability was obtained and the following excavation has been operated successfully until now.

• Journal of the Society of Disaster Information
• /
• v.14 no.3
• /
• pp.367-378
• /
• 2018

Purpose: The objective of this study is to present a reasonable safety management of the anchored in-situ wall systems constructed in the ground conditions consisting of multi-layered soils underlain by bedrocks in the urban area of Korea. Method: Field measurements collected from collapse case histories with deep excavations were analyzed for the safety management of the wall systems supported by the earth anchors in terms of lateral displacement rates. Results: The average maximum lateral displacement rate in a collapsed zone of the in-situ wall significantly increased upon the completion of the excavation. Particularly, the collapse of the in-situ wall system due to the sliding occurring along the discontinuities of the rock produced a considerably large lateral displacement rate over a relatively short period. Conclusion: For predicting and preventing the collapse of the wall system during or after the excavation work, the utilization of the safety management criteria of the in-situ wall system by the lateral displacement rate was found to be much more reasonable in judging the safety of earthworks than the application of the quantitative management criteria which have been commonly used in the excavation sites.

• Journal of Hydrogen and New Energy
• /
• v.20 no.3
• /
• pp.208-215
• /
• 2009

PEMFC stack power output is needed to be around 100 kW to meet the requirements of automotive application and scaling-up the active area of the stack cells will allow a higher power. In the case of scaling-up the active area of cells, it is difficult to obtain uniform in-plane internal conditions such as temperature, relative humidity and stoichiometry of the feed gas. These ununiformity with the location in the cell would affect both the performance and durability of the stack, so it is important to understand phenomena in the cell for improving them. In this study, the current density, electrochemical resistance and performance distribution measurement was performed to understand the ununiformity in a single cell using in-situ method; (1) Current Density Distribution (CDD) Device and (2) Segmented Cell Fixture. The influence of location of feed gas on the performance of a single cell was experimentally measured and discussed by using a segmented single cell which was composed of 8 compartments. The correlation between the location and performance in a single cell was discussed by these two tools and it was extended between the local characterization and the durability in a MEA by comparing the used cell with a fresh one. It was also studied in terms of electrochemistry by Electrochemical Impedance Spectroscopy.

• Applied Chemistry for Engineering
• /
• v.26 no.3
• /
• pp.336-340
• /
• 2015

Three different types of polycarbonate (PC)/graphene oxide (GO) composites using diphenyl carbonate as a monomer were fabricated by melt polymerization. Those were the PC/GO composite (PC/GO) using a twin extruder, in-situ PC/GO composite (PC/GO-cat.) using a catalyst, and in-situ PC/GO composite (PC/GO-COCl) using a GO-COCl treated by -COCl, Chemical structures of the composites were confirmed by C-H and C=O stretching peak at $3000cm^{-1}$ and $1750cm^{-1}$, respectively. The slope for the storage (G') versus loss (G") modulus plot decreased with an increase in the heterogeneous property of polymer melts. So we can check the GO dispersion of the PC/GO composites using by the slop for G'-G" plot. According to the G'- G" slopes for three different types of PC/GO composites, GO was well dispersed within PC matrix in case of PC/GO and PC/GO-cat.. It was also confirmed by atomic force microscope (AFM) photos. One of the reasons for the poor GO dispersion of PC/GO-COCl is branching and crosslinking processes occurred during polymerization, which was further confirmed by a plot for the complex modulus versus phase difference.

• Journal of Aquaculture
• /
• v.10 no.2
• /
• pp.171-178
• /
• 1997

Infectious pancreatic necrosis virus (IPNY) is an economically important fish pathogen since it causes the high-mortality disease in early stage of hatchery-reared fishes. In order to develop a rapid, sensitive and highly specific detection method for IPNV, reverse transcription-polymerase chain reaction (RT-PCR) was carried out using the oligonucleotide primers selected from the sequence of VP2, a major capsid polypertide of IPNV. As little as 40ng of purified IPNV dsRNA was detected by RT-PCR amplification, but no amplification products were obtained when nucleic acid genomes from other fish pathogens such as IHNV were used as RT-PCR templates. in situ RT-PCR methods are useful for the rapid and sensitive identification of IPNV.

• Journal of the Korean Society for Heat Treatment
• /
• v.6 no.1
• /
• pp.17-25
• /
• 1993

The Cu - $Nb_3Sn$ composites wire as a superconducting material was prepared by in situ method as follow: Cu - 15wt.% Nb alloys which were melted in a high -frequency induction furnace and casted in bar were cold-worked up to the final diameter of 0.24 mm, electroplated with Sn, pre-treated in two steps and then diffused at $550{\sim}650^{\circ}C$ for 24 ~ 96 hrs. The overall $J_c$ and $T_c$ of the specimens were measured by the four point-probe method at 10 K in the magnetic field of 0 Tesla. The overall $J_c$ of the composites wire which diffused at $550^{\circ}C$ after pre-treating in two steps were generally higher than those of the wire at either $600^{\circ}C$ or $650^{\circ}C$. For the specimens diffused at $550^{\circ}C$, the overall $J_c$ were increased until 72 hrs. of diffusion time and then decreased. However, in case of diffusion at $600^{\circ}C$ and $650^{\circ}C$, the overall $J_c$ were gradually decreased from the beginning. The maximum overall $J_c$ obtained in this experiment was $1.3{\times}10^4\;A/cm^2$, which was measured for the specimen diffused at $550^{\circ}C$ for 72 hrs. When the specimens were diffused at $550^{\circ}C$ for 72 hrs, after pre-treating, the measured critical temperature, $T_c$ was 16.19 K. Similar $T_c$ value were obtained in other specimens regardless of diffusion time and temperature.

• The Korean Journal of Physiology and Pharmacology
• /
• v.14 no.2
• /
• pp.71-75
• /
• 2010

To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by western blotting serosal side solution using rat everted gut sacs method showed that lumbrokinase proteins between 33.6 and 54.7 kDa are absorbed mostly by the intestinal epithelium. Furthermore, MALDI- TOF mass spectrometric analysis of plasma fractions obtained by in situ recirculation method confirmed that lumbrokinase F1 is absorbed into blood. These results support the notion that lumbrokinase can be absorbed from mucosal lumen into blood by oral administration.

• Korean Journal of Microbiology
• /
• v.34 no.3
• /
• pp.169-174
• /
• 1998

To define the structure and diversity of bacterial communities in the aqutic ecosystem, Lake Soyang, the largest artificial reservoir in Korea, a new method, fluorescent in situ hybridization was applied. This technique relies on the specific hybridization of the nucleic acid probes to the naturally amplified intracellular rRNA. By this method, the bacterial community composition of Lake Soyang and bacterial numbers belong to eubacteria, proteobacteria and Cytophaga-Flavobacterium group were estimated. Total bacterial numbers ranged from $0.3{\times}10^6{\sim}2.0{\times}10^6cells{\cdot}ml^{-1}$, and vertical profile of total bacteria showed the peak at 2 and 5 m depths. The ratio of eubacteria to total bacteria were 22~100% and varied with depth and season. The percentage of Proteobacteria ${\alpha}$-group ranged 2.6~66.7%, ${\beta}$-group 4.5~53.5%, ${\gamma}$-group 4.6~76.7% and Cytophaga-Flavobacterium group 2.1~35.9%. Also, bacteria] community had spatial and temporal characteristics. The dominant groups were ${\beta}$-group in winter, ${\gamma}$-group in spring and early summer and ${\alpha}$-group in summer.