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http://dx.doi.org/10.4196/kjpp.2010.14.2.71

Intestinal Absorption of Fibrinolytic and Proteolytic Lumbrokinase Extracted from Earthworm, Eisenia andrei  

Yan, Xiang Mei (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Kim, Chung-Hyo (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Lee, Chul-Kyu (Central Research Institute, Shin Poong Pharm. Co. Ltd.)
Shin, Jang-Sik (Central Research Institute, Shin Poong Pharm. Co. Ltd.)
Cho, Il-Hwan (Central Research Institute, Shin Poong Pharm. Co. Ltd.)
Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.14, no.2, 2010 , pp. 71-75 More about this Journal
Abstract
To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by western blotting serosal side solution using rat everted gut sacs method showed that lumbrokinase proteins between 33.6 and 54.7 kDa are absorbed mostly by the intestinal epithelium. Furthermore, MALDI- TOF mass spectrometric analysis of plasma fractions obtained by in situ recirculation method confirmed that lumbrokinase F1 is absorbed into blood. These results support the notion that lumbrokinase can be absorbed from mucosal lumen into blood by oral administration.
Keywords
Eisenia andrei; Fibrinolytic enzymes; Intestinal proteinase absorption; Gut sac; Recirculation intestinal perfusion;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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