• Molecules and Cells
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• v.45 no.7
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• pp.502-511
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• 2022

Bacterial volatile compounds (BVCs) exert beneficial effects on plant protection both directly and indirectly. Although BVCs have been detected in vitro, their detection in situ remains challenging. The purpose of this study was to investigate the possibility of BVCs detection under in situ condition and estimate the potentials of in situ BVC to plants at below detection limit. We developed a method for detecting BVCs released by the soil bacteria Bacillus velezensis strain GB03 and Streptomyces griseus strain S4-7 in situ using solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS). Additionally, we evaluated the BVC detection limit in the rhizosphere and induction of systemic immune response in tomato plants grown in the greenhouse. Two signature BVCs, 2-nonanone and caryolan-1-ol, of GB03 and S4-7 respectively were successfully detected using the soil-vial system. However, these BVCs could not be detected in the rhizosphere pretreated with strains GB03 and S4-7. The detection limit of 2-nonanone in the tomato rhizosphere was 1 µM. Unexpectedly, drench application of 2-nonanone at 10 nM concentration, which is below its detection limit, protected tomato seedlings against Pseudomonas syringae pv. tomato. Our finding highlights that BVCs, including 2-nonanone, released by a soil bacterium are functional even when present at a concentration below the detection limit of SPME-GC-MS.

• Journal of Korean Tunnelling and Underground Space Association
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• v.4 no.1
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• pp.57-70
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• 2002

Since early in the 90's, the need for construction of underground rock structures such as long and large section traffic tunnel, energy storage cavern, industrial facility, etc. has been largely increased because the Korean territory is not wide and about 65 % of the land consists of mountainous region. The initial rock stress measurement has been widely conducted to provide the quantitative information on the stress state of engineering site at the design stage of underground rock structures. Among the diverse methods developed for measuring rock stress, hydraulic fracturing test is most popularly used because it is applicable at pre-construction stage and has no limit in testing depth. In this paper, the characteristics of initial rock stress state in mountainous region were studied on the basis of the in-situ hydraulic fracturing stress measurement results from the 60 test boreholes in various parts of Korea.

• Journal of the Korean Ceramic Society
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• v.34 no.8
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• pp.803-810
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• 1997

Ba0.5Sr0.5TiO3 thin films were deposited by RF magnetron sputliring method in order to clarify the anneal condition and doping effect on loakage current Nb and Al were selected as electron donor and acceptor dopants respectively, in the BST films because they have been known to have nearly same ionic radii as Ti and thought to substitute Ti sites to influence the charge carrier and the acceptor state adjacent to the gram boundary. BST thin films prepared in-situ at elevated temperature showed selatively high leakage current density and low breakdown voltage. In order to achieve smooth surface and to improve electrical properties, BST thin films were deposited at room temperature and annealed at elevated temperature. Post-annealed BST thin films showed smoother surface morphology and lower leakage current density than in-situ prepared thin films. The leakage current density of Al doped thin films was measured to be around 10-8A/cm2, which is much lower than those of undoped and Nb doped BST films. The result clearly demonstrates that higher Schottky barrier and lower mobile charge carrier concentration achieved by annealing in the oxygen atmosphere and by Al doping are desirable for reducing leakage current density in BST thin films.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.19 no.1
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• pp.75-80
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• 2006

We have fabricated a carbon nanotube field emission display(CNT-FED) panel with a 2 inch diagonal size using a screen printing method and in-situ vacuum sealing technology. The field emission properties of CNT FED panel with square-type CNT emitters. As results, the square-characterized and compared with those of the line-type CNT emitters. As results, the square-type CNT emitters showed much larger emission current and more stable I-V characteristics. Light emission started to be occurred at an electric field of 3.5 V/${\mu}m$ corresponding to the anode-cathode voltage of 700 V. The vacuum level inside of the in-situ vacuum sealed panel was obtained with $1.4 {\times} 10^{-5}$ torr. The sealed panel showed the similar I-V characteristics with the unsealed one and the uniform light emission with very high brightness at a current density of $243 {\mu}A/ cm^2$ obtained by the electric field of 10 V/${\mu}m$.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.11b
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• pp.171-175
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• 2001

Chemical mechanical polishing(CMP) process has been widely used to planarize dielectrics and metal, which can apply to employed in integrated circuits for sub-micron technology. Despite the increased use of CMP process, it is difficult to accomplish the global planarization of free-defects in inter level dielectrics and metal. Especially, defects like (micro-scratch) lead to severe circuit failure, and affects yield. Current conditioning method - bladder type, orbital pad motion- usually provides unsuitable pad profile during ex-situ conditioning near the end of pad life. Since much of the pad wear occurs by the mechanism of bladder type conditioning and its orbital motion without rotation, we need to implement new ex-situ conditioner which can prevent abnormal regional force on pad caused by bladder-type and also need to rotate the pad during conditioning. Another important study of ADPC is related to the orbital scratch of which source is assumed as diamond grit dropped from the strip during ex-situ conditioning. Scratch from diamond grit damaged wafer severely so usually scraped. Figure 1 shows the typical shape of scratch damaged from diamond. e suspected that intensive forces to the edge area of bladder type stripper accelerated the drop of Diamond grit during conditioning. so new designed Flat stripper was introduced.

• Tunnel and Underground Space
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• v.18 no.2
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• pp.149-161
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• 2008

It is nearly impossible to estimate the exact state of the current rock stress of interest site by the theoretical and physical approaches except some specific geological situations. This means that in-situ stress measurement is a unique way to obtain reliable information on rock stress especially for civil and mining engineering related problems. Since late in the 90's, in-situ rock stress tests have been widely conducted to provide the quantitative information on the stress state of engineering site at the design stage of an underground rock structure in the Kyungsang Basin, Korea. The study area is the near surface regions at the depth less than 300 m in the Kyungsang Basin. It includes Yeosoo to the west and Busan to the east. Totally, 270 in-situ stress measurements were conducted in the surface test boreholes at the depth from 14 m to 300 m by hydraulic fracturing method. In this paper, based on the measurement data set, the overall characteristics of the current in-situ rock stress fields in the study area are briefly described. And also the investigation results on the difference between the stress distributions for the granitoid and the andesitic rock region are also introduced. Finally, the distributions of the regional horizontal stress directions in Busan and the Yangsan faults area are shown.

• Korean Journal of Materials Research
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• v.17 no.1
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• pp.11-17
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• 2007

Turbulent in-situ mixing process is a new material process technology to get dispersed phase in nanometer size by controlling reaction of liquid/solid, liquid/gas, flow ana solidification speed simultaneously. In this study, mixing which is the key technology to this synthesis method was studied by computational fluid dynamics. For the simulation of mixing of liquid metal, static mixers investigated. Two inlets for different liquid metal meet ana merge like 'Y' shape tube having various shapes and radios of curve. The performance of mixer was evaluated with quantitative analysis with coefficient of variance of mass fraction. Also, detailed plots of intersection were presented to understand effect of mixer shape on mixing. The simulations show that the Reynolds number (Re) is the important factor to mixing and dispersion of $TiB_2$ particles. Mixer was designed according to the simulation, and $Cu-TiB_2$ nano composites were evaluated. $TiB_2$ nano particles were uniformly dispersed when Re was 1000, and cluster formation and reduction in volume fraction of $TiB_2$ were found at higher Re.

• Journal of the Korean Geosynthetics Society
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• v.11 no.1
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• pp.47-56
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• 2012

Discharge capacity of PBD is sensitive in proportion to thickness and ground condition, and drainage of PBD declines due to disturbance effect in surrounding ground by mandrel used for vertical drainage setting and setting machines and type. Also, deviation of discharge capacity gets larger according to ground condition, construction condition and soil properties. But cause and analysis of those problems like reduced discharge of capacity and delayed dissipation of pore water pressure for discharge capacity is lack. Thus, in this text, ground improvement and discharge capacity is investigated by implementing composite discharge capacity test for analysis of an effect factor of PBD discharge capacity with in-situ ground condition. After fixing the vertical drain on a cylindrical cylinder, put churned sample into the cylinder. After in-situ ground and reclamation of ground are dredged, load following the loading step of 30, 70 and 120kPa using a pressure device. Result of the test, The discharge capacity was SM>ML>CL>CL(dredged soil) in situ condition and more fine-grained content, the amount of discharge was greater.

• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.583-592
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• 1999

We have developed the in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues or cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies were observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.

• Tunnel and Underground Space
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• v.17 no.2 s.67
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• pp.90-101
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• 2007

With increasing development of underground space, underground pumped storage power plants, which generate power by felling water in upper reservoir to lower reservoir, have been continuously constructed. For efficient and safe design, construction and maintenance or such power plants, it is very important to understand in-situ stress and the mechanical properties of the surrounding rock mass at the design stage. The power plant presented in this paper is under construction at a depth of $320{\sim}375m$. For stability evaluation of the structure, in-situ stress was measured by over-coring method. Also pressurementer test and a series or laboratory tests were performed to obtain the mechanical properties. Numerical analyses were conducted to check the efficiency of designed support patterns. The results showed that unstable areas occurred partly in the numerical model, and therefore supports were additionally applied. Finally complete stability was obtained and the following excavation has been operated successfully until now.