• The Journal of Pediatrics of Korean Medicine
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• v.22 no.1
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• pp.113-121
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• 2008

Objectives In human myeloid leukemia cells, there are no specific features of apoptosis compared with apoptosis in other cell types. Solanum nigrum L.(SNL) is a deciduous tree, which is widely distributed in Korea with reported anti-tumor, anti-inflammatory and non-specific immune-enhancing properties. Although the plant has been clinically used for treating a variety of diseases, its bioactive ingredients are unknown and its mode of action potential has never been investigated. Thus anti-tumor property of methanol extract was investigated. Methods In this study, anti-tumor property of methanol extract was investigated by determining its in vitro growth-inhibitory effects on human myeloid leukemia cells. XTT proliferation assay, DNA fragmentation, immunoblot analysis, densitometric analysis were used. Results 1. The methanol fraction of the extracts of SNL induced mitochondria-mediated apoptosis in human myeloid leukemia cells. 2. The methanol fraction exhibited relatively higher cytotoxic activity in a dose-dependent manner than chloroform, and hexane fraction. 3. Typical ladder profile of Oligonucleosomal fragments were appeared. 4. The secreted cytosolic cytochrome C level was increased by treatment of methanol fraction. Conclusions Methanol fraction of SNL is capable of inducing apoptosis in human myeloid leukemia cells.

• Food Science and Biotechnology
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• v.18 no.4
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• pp.833-841
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• 2009

Monoterpenes, in special limonene and its derivatives, are well studied in the literature due to their several properties. They are well recognized as major components of essential oils; some of them, are important industry residues, and others present some important biological activities. In this review, the biotransformation of the inexpensive limonene into flavor compounds was briefly reviewed and the main pathways for limonene biotransformation are presented. Furthermore, some important biological properties of these compounds were also considered, like bactericidal activity, induction of immune response, and role in disease prevention, with a little emphasis on some possibilities related to the mechanisms of anticancer action.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.6
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• pp.1223-1229
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• 2002

The purpose of this research was to investigate the effect of Cheongsimondam-tang(CSODT) on the activity of immune cell and anti-carcinogenic effect of mouse leukemia cell. The addition of CSODT(1 ㎍/ml) enhanced the proliferation of cultured-splenocytes and thymocytes. And also administration of CSODT(500 ㎍/kg) accelerated subpopulation of splenic and thymic T lymphocytes especially CD4/sup +/-T/sub H/ cells in BALB/c mice. CSODT treatment decreased cell proliferation and increased apoptotic cell death of cultured-L1210 leukemia cells, and induced apoptosis in addition to decreased mitochondrial transmembrane potential (ΔΨm) of transplanted-L1210 cells in vivo. These results suggest that CSODT have a cellular immuno-regulatory effect and anti-cancer property action.

• Journal of Advanced Marine Engineering and Technology
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• v.33 no.7
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• pp.981-986
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• 2009

The application of a cryogenic storage system is growing fast in different kinds of fields including to keep umbilical cord blood. Umbilical cord blood stem plays an important role in the treatment of a blood and immune system related genetic diseases, cancers and blood disorders. This study gives the optimal cryogenic system for cord blood banking. Three-dimensional models are employed and finite element method is used to do structure analyses of all designed models. The results shows model 3 have a good structure properties, and model 4 shows the best structure property as its maximum is 92.9 MPa. The other is too dangerous or infeasible to support load condition that allowed by STS 304. The results can be used in the design of these kinds of systems to obtain good predictions of trends over a wide range of design alternatives and operating conditions.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.416-417
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• 2000

Chitin, a poly $\beta$-(1longrightarrow14)-N-acetyl-D-glucosamine, is best known as a cell wall component of fungi and as a skeletal materials of invertebrates. Chitosan is derived from chitin by deacetylation in the presence of alkali. Chitosan has been developed as new physiological materials since it possesses antibacterial activity, hypocholesterolemic activity and antihypertensive action. However, the actions of chitosan in vivo still remain ambiguous as the physiological functional properties because most animal intestines, especially the human gastrointestinal tract, do not possess enzyme such as chitosanase which directly degrade the $\beta$-glucosidic linkage in chitosan, and consequently the unbroken polymers may be poorly absorbed into the human intestine. Therefore, recent studies as chitosan have attracted interest for chitosan oligosaccharides, because the oligosaccharides process not only water-soluble property but also versatile functional properties such as antitumor activity, immune-enhancing effects, enhancement of protective effects against infection with some pathogens in mice and antimicrobial activity (Kingsnorth et al., 1983, Mori et al., 1997). (omitted)

• Proceedings of the Korean Institute of Building Construction Conference
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• 2018.11a
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• pp.151-152
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• 2018

The purpose of this study was to find out harmful effects of concrete, an essential material in modern architecture, on the human body. Based on the results from animal testing, we can consider the effects on the human body. The results of this study were as follows; Experimental Mouses in concrete have low body temperature due to cold radiation and more aggressive due to cold stress. Therefore, Cold radiation, a property of concrete, makes body temperature lower and affect the body's immune function.

• IMMUNE NETWORK
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• v.13 no.4
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• pp.133-140
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• 2013

Since the discovery of the immunomodulation property of mesenchymal stem cells (MSCs) about a decade ago, it has been extensively investigated whether MSCs can be used for the treatment of immune-related diseases, such as graft versus-host disease (GvHD). However, how to evaluate the efficacy of human MSCs for the clinical trial is still unclear. We used an MHC-mismatched model of GvHD (B6 into BALB/c). Surprisingly, the administration of the human MSCs (hMSCs) could reduce the GvHD-related mortality of the mouse recipients and xenogeneically inhibit mouse T-cell proliferation and $IFN-{\gamma}$ production in vitro. We recently established a new protocol for the isolation of a homogeneous population of MSCs called subfractionation culturing methods (SCM), and established a library of clonal MSC lines. Therefore, we also investigated whether MSCs isolated by the conventional gradient centrifugation method (GCM) and SCM show different efficacy in vivo. Intriguingly, clonal hMSCs (hcMSCs) isolated by SCM showed better efficacy than hMSCs isolated by GCM. Based on these results, the MHC-mismatched model of GvHD may be useful for evaluating the efficacy of human MSCs before the clinical trial. The results of this study suggest that different MSC lines may show different efficacy in vivo and in vitro.

• Biomolecules & Therapeutics
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• v.17 no.3
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• pp.293-298
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• 2009

Korean mistletoe lectin (KML) is the major component found in Viscum album var. (coloratum), displaying anti-cancer and immunostimulating activities. Even though it has been shown to boost host immune defense mechanisms, the regulatory roles of KML on the functional activation of macrophages have not been fully elucidated. In this study, regulatory mechanism of KML on macrophage-mediated immune responses was examined in terms of KML-mediated signaling event. KML clearly induced mRNA expression of tumor necrosis factor (TNF)-$\alpha$, the generation of reactive oxygen species (ROS) and phagocytic uptake in RAW264.7 cells. All of these events were strongly suppressed by U0126, whereas TNF-$\alpha$ mRNA was not diminished by SB203580 and SP600125, indicating ERK as a central enzyme managing KML-induced up-regulation of macrophage functions. Indeed, KML strongly induced the phosphorylation of ERK in a time-dependent manner without altering its total level. Therefore, these data suggest that ERK may be a major signaling enzyme with regulatory property toward various KML-mediated macrophage responses.

• The Journal of Pediatrics of Korean Medicine
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• v.35 no.1
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• pp.76-103
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• 2021

Objectives This study was designed to examine the effect of Gleditsiae Fructus n-hexane (GSF_Hx) on two different groups (on the LPS-induced activation of Raw264.7 cells in vitro, and on the DNCB-induced activation of atopic dermatitis NC/Nga Tnd mice in vivo) to find index components and active components of Gleditsiae Fructus. Methods GSF_Hx was analyzed by HPLC profiling and confirmed echinocystic acid (EA), oleanolic acid (OA) as index components of Gleditsiae Fructus. Using GSF_Hx, EA, OA, we investigated IL-6, TNF-α, NO production by ELISA analysis and evaluated manifestations of MAPKs transcription factors and NF-κB p65 translocation by western blotting. During In vivo study, atopic dermatitis was induced on NC/Nga Tnd mice by DNCB and administered GSF_Hx, EA, OA orally, and checked skin lesions and measured skin clinical score. Serum IgE level, Th1 and Th2 cytokines secretion and modulating molecular mediators and immune cells in the spleenocyte culture supernatant, PBMCs, ALN and dorsal skin were also measured by real-time PCR. Then, skin rash was evaluated and mast cell distribution was verified by H&E and toluidine blue staining on dorsal skin. Results It is possible that GSF_Hx, EA and OA reduce inflammation and allergic response of atopic dermatitis by suppressing Th1 and Th2 cytokines secretion and modulating molecular mediators and immune cells. They also had moisturizing effect by raising vitality of ceramide in dorsal skin of atopic dermatitis NC/Nga Tnd mice. However, EA particularly had better overall activity data than OA, that EA could be a more effective active component of Gleditsiae Fructus than OA. Conclusions Based on the inflammatory reduction property with moisturizing effect, GSF_Hx may play a role in effective treatment for atopic dermatitis.

• Korean Security Journal
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• no.60
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• pp.253-282
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• 2019

College students majoring in bodyguards learn and acquire bodyguard martial arts to protect the life and property of the subjects. Also, the bodyguard martial arts are helping to improve and maintain the body and spirit of the bodyguards. However, more and more students majoring in security at universities are becoming obese due to lack of time to participate in bodyguard training and excessive food intake, which could have a negative impact on their future security work as well as their health condition. In this study, the effect of 10 weeks of Judo training on body composition, blood lipid and immune function on Security Majoring obese male college students was determined to be as follows. 1. Weight(p<.05), ; body fat ratio(p<.05), BMI(p<.01) etc. of body composition after Judo training were significantly reduced, and in the case of BMI(F=4.488, p<05), there were significant differences in interaction between groups and timing. 2. After judo training, TC (p<.05) and LDL-C(p<.01) of the blood lipid showed a significant decrease. 3. After judo training, lymphocytes of leukocytes were shown to increase significantly(p<.05), lymphocytes(F=11.772, p<.01) showed significant differences among groups. 4. After judo training, IgA and IgG of immunoglobulin were shown to increase significantly (p<.05), IgA(F=7.646, p<.05) and IgG (F=14.267, p<.01) showed significant differences among groups. Considering the above results, it is thought that judo training can prevent obesity due to reduced body fat and reduce neutral fat and cholesterol, thus preventing arteriosclerotic diseases. In addition, it is expected to play a positive role in defense against human bacterial infections, inflammatory reactions, and external antigen in immune function. It is thought that this will not only prevent the bodyguards' obesity but also maintain a healthy life and improve the security work efficiency. However, some studies have shown results that are somewhat different from the results of this study, so studies on blood lipids, immune function, and exercise will require more detailed research on various methods of exercise, such as exercise time and intensity.