• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.2
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• pp.231-238
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• 2011

Attacin is an insect antibacterial protein that plays an important role in immune response to injury and infection. In this report, we have isolated and characterized of cDNA encoding for the attacin from the immunized larvae of swallowtail butterfly, $Papilio$ $xuthus$. A full length cDNA of $P.$ $xuthus$ attacin was obtained by employing annealing control primer (ACP)-based differential display PCR and 5' RACE. The complete $P.$ $xuthus$ attacin cDNA was comprised of 949 bp encoding a 250 amino acid precursor. It contains a putative 18 amino acid signal peptide sequence, a 42 amino acid propeptide sequence, and a 190 amino acid mature protein with a theoretical molecular mass of 19904.01 and a pI of 9.13. The putative mature protein of $P.$ $xuthus$ attacin showed 48-52% and 24-30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. Semiquantitive RT-PCR results revealed that the transcript of $P.$ $xuthus$ attacin gene was up-regulated at significant levels after injection with bacterial lipopolysaccharide (LPS). We sub-cloned cDNA fragment encoding mature $P.$ $xuthus$ attacin into the expression vector, highly expressed in $E.$ $coli$ BL21 cells, and its antibacterial activity was analyzed. Recombinant $P.$ $xuthus$ attacin evidenced considerably antibacterial activity against Gram-negative bacteria, $E.$ $coli$ ML 35 and $Klebsiella$ $pneumonia$.

• Korean Journal of Occupational Health Nursing
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• v.20 no.3
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• pp.250-260
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• 2011

Purpose: The purpose of this study was to describe the role experiences of occupational health nurse. The research question was "what are daily experiences in practice?" Methods: The data were collected through the focus-group interviews with nine occupational health nurses and analyzed by the grounded theory of Strauss and Corbin (1998). Results: The core category on occupational health nurse role experience was named as "struggling alone with the dual role". The causal conditions included 'unfamiliar area' and 'new work'. The contextual conditions included 'overload of work, 'tasks beyond OHN job' and 'disharmony with colleagues'. The intervening conditions were analyzed as supportive factors and discouraging factors. There were action/interaction strategies 'getting information about tasks', 'working as a professional', 'establishing a cooperative relationship', 'seeking help' by trying to regulate the phenomenon. Finally they succeed in 'establishing the identity' and 'feeling proud', but sometimes they could be 'suffering from low morale', or 'considering turnover'. Conclusion: Through these results, it can be explained that occupational health nurses have been exerting themselves to establish their role with various situation through the conflicted context. Based on the findings, we suggest developing the diverse continuing education programs to be tailored for OHN's needs and reinforcing the occupational health services under the legal and political support.

• Korean Journal of Social Welfare
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• v.69 no.2
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• pp.219-246
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• 2017

The purpose of this study is to develop a substantive theory that understand the burnout and secondary traumatic stress experience of social workers who worked in the trauma centers. This research was conducted by Grounded Theory. The result is as follow: In the initial coding, 159 units of mean, 47 subcategories, and 12 main categories. The central phenomenon of the initial coding was 'the wounded existence.' In the focused coding, the core category was 'seeking to recover professional identity as a social worker.' In the theoretical coding, the burnout and secondary traumatic stress experience of social workers who worked in the trauma centers were classified as four phases;'Commitment to the mission', 'Conflict', 'Stagnant' and 'Disruption'. Based on the findings, the preparation for the disaster social work practice, the perspective on "social healing" of trauma, and the necessity of the mandatory policy or intervention for social workers self-care were discussed.

• The Research Journal of the Costume Culture
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• v.20 no.1
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• pp.83-98
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• 2012

In 2009, two national universities were integrated into one and the following was newly named Gangneung- Wonju National University. Therefore, this university requires consolidation and promotion of UI(University Identity) as a college brand in order to secure and enhance its competitiveness. The purpose of this study was to design and develop campus-wear, ultimately, it can be used as a part of the Gangeung-Wonju University's UIP policy. A qualitative approach of searching==incorporating books, dissertations, and web-based information==was taken to investigate theoretical backgrounds. The campus-wears were collected from the selected universities in Korea and abroad for the distinct design development. The result of the research are as follows. The concept for the design planning was "wit, exciting & challenge for campus life". The developed UI had focused on expressing positive images of the University. Six items were chosen for the campus-wear design. The goal was to demonstrate practicality and fashion trend. The color arrangement primarily focused on the University's official color and pursued a bright, breeze look. The detailed techniques used for the actual production were printing, embroidery, and accessories. The UI products may not only instill the members of the university with the spirit and pride by strengthening the sense of belonging, but also create a synergistic effect in education by boosting the university promotion and by newly establishing standing as the integrated university.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.127-131
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• 2016

Unusual mushrooms were observed in strawberry and sweet pepper greenhouses in the Hwasun area of Korea in September 2015. The mushrooms usually grew on beds in the greenhouses, frequently along with vegetables. The identity of the mushrooms in the greenhouses and effects of the mushrooms on the growth and quality of vegetables were investigated. Mushroom specimens collected were identified based on their morphological and molecular characteristics. The mushroom specimens collected from the strawberry greenhouse were identified as Leucocoprinus birnbaumii and L. ianthinus, and those from the sweet pepper greenhouse as Gymnopilus lepidotus. These were the first observations of L. ianthinus and G. lepidotus in Korea. The results revealed that the mushrooms originated from coco peat media used for the beds in the greenhouses. The mushrooms did not affect the growth and quality of the two vegetables.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.411-418
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• 2015

Lonicera caerulea var. edulis is a rare species found in some alpine region of Korea. Genetic variation in L. caerulea var. edulis has been investigated by examining 161 individuals from six natural populations: Mt. Seorak 1, Mt. Seorak 2, Mt. Jeombong, Mt. Bangtae, Mt. Gyebang, Mt. Halla. The mean genetic diversity for all the six populations was 0.25 (S.I.). The highest genetic diversity was found in Mt. Seorak (S.I.=0.3158) and the lowest was in Mt. Gyebang (S.I.=0.1047). Comparatively low level of genetic diversity was observed (Ae=1.25, P= 64.6%, S.I.=0.25), which is a typical pattern for rare tree species. AMOVA showed exceptionally large proportion of genetic variations both for among populations (34.69%) and within populations (65.31%). Excluding Mt. Gyebang, the genetic variation among and within population was 18.71% and 81.29% respectively. The UPGMA dendrogram based on genetic distance is not suitable for geographic relationship. Genetic distance of Mt. Gyebang was most distant from the other populations. Excluding Mt. Gyebang, the genetic identities among the five populations were 0.95 to 0.97, which is very high similarity level of genetic identity. This low level of genetic variations and the lack of site in nature indicates that Lonicera caerulea var. edulis demanded a serious conservation.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2005.11a
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• pp.167-170
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• 2005

This study aims to develop a detachable 'Infill Components' applicable to open housing. Recently, the need for innovative housing methods is increasing because of the environmental preservation issues and the need for favorable housing stock resulting from the increased housing supply ratio. In order to maintain favorable housing stock, there has to has a to be a shift from typical plans and construction methods for mass production to those with some identity, which may satisfy various needs of dwellers. In this light, the Ramen structure has become popular owing to the growth of remodelling market, and construction companies tend to adopt flexible type multi-family housings to increase sales by appealing to their customers. However, there are few domestic studies on the Infill components for the change of structure. As a result, further studies may have to be based on the case study. The purpose of this research is to provide fundamentals for the development of infill components corresponding to the structural change, especially for the development of partition walls that can be easily moved by dwellers. By reviewing problem of construction, arrangement of the movable partition wall system and door system which has within wring in the first Experimental Open Housing in Korea at KICT(KOHP21), this research provides the fundamentals for developing a movable partition wall acceptable to the dwellers who may want to remodel the interior to meet the needs of themselves.

• Journal of the Korea Convergence Society
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• v.8 no.6
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• pp.227-235
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• 2017

The essence of architecture is space acquired as a result of architect's design act, but the object of the design is not the space and it is the physical elements to construct it. The masters of modern architecture have described the types of spaces that can be composed of these elements, but there is a difficulty in constructing the space because they does not mention how to construct it. From this point of view, if architecture is the techniques for human behavior and the art of building space, it is recognized that there is a need for concrete research on how to construct a space with ambiguous substance. Therefore, I conducted research on the extensibility of diagonal spaces through '4 Logements Studio' by Henri Ciriani, a representative architect of Neo Corbusian. The 'network for identity' method proposed by Ciriani was examined as a methode to realize spatial expansion and the furniture should be designed as an element of reading space and 3 optimal alternatives for 4 housing arrangements were obtained based on the Equivalence, Lighting, View, and Community life.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.312-317
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• 2002

Indigenous lactobacilli were isolated from vaginas of Korean women for possible use in ecological treatment of bacterial vaginosis. Vaginal swab samples were obtained from a gynecological clinic and streaked on Rogosa SL agar plates to select the most predominant lactobacilli in each sample. The preliminary identification of the isolates as lactobacilli was based on microscopic observation of Gram-positive rod-shaped cell morphology. The initial characterization was performed on 108 isolates in terms of their cell surface hydrophobicity (CSH), antimicrobial activity, and hydrogen peroxide (H₂O₂) production capability, and 10 isolates were then selected for further molecular identification. For a rapid procedure to identify lactobacilli, polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses of the l6S rRNA genes were applied. The 10 selected lactobacilli and 9 different reference strains of Lactobacillus spp. were characterized by PCR-RFLP where the amplified l6S rDNA was digested with 7 different restriction endonucleases prior to analysis. DNA sequencing of the 16S rRNA gene of one particular isolate, KLB 46, that had been identified as L. crispatus by the PCR-RFLP analysis, further confirmed its identity as L. crispatus.

• Journal of Navigation and Port Research
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• v.39 no.4
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• pp.329-334
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• 2015

Currently, image of floating architecture has been positively improved based on the normalization of a domestic representative floating building 'Some Sevit'. Features of the floating architecture are as follows: special experience (29.9%)> landmark (27.6%)> enjoyment of marine culture (21.5%)> center for tourism and regional development (16.0%)> eco-friendly space solving global warming (4.8%). Floating building has a distinctive image and at the same time offers a unique spatial experience to the public. Therefore, space design marketing of floating building is a communication process to exchange its spatial identity and image between the local government and the public, the corporation and customers. It is essential for the effective space design marketing that the spatial demands should be reflected into its program such as commercial, cultural and marina facilities. The unification of project leader and operator is also important. The transformed conditions would help the construction market to be active in the future.