• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.854-861
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• 2014

The diagnosis of Brucella abortus is mainly based on serological methods using antibody against LPS, which has diagnostic problems. Therefore, to solve this problem, we evaluated two proteins of B. abortus, Cu/Zn superoxide dismutase (SodC) and outer membrane proteins 2b porin (Omp2b). The genes were cloned and expressed in a pMAL system, and the recombinant proteins, rOmp2b and rSodC, were purified as fusion forms with maltose-binding protein. The identity of the proteins was confirmed by SDS-PAGE and Western blot analysis with sera of mice infected with B. abortus. Production of cytokines and nitric oxide (NO) was investigated in RAW 264.7 cells and mouse splenocytes after stimulation with the proteins. Moreover, cellular and humoral immune responses were investigated in BALB/c mice after immunization with the proteins. TNF-${\alpha}$, IL-6, and NO were significantly inducible in RAW 264.7 cells. Splenocytes of naive mice produced IFN-${\gamma}$ and IL-4 significantly by stimulation. Moreover, number of IgG, IFN-${\gamma}$, and IL-4 producing cells were increased in immunized mice with the two proteins. Production of IgG and IgM with rOmp2b was higher than those with rSodC in immunized mice. These results suggest that the two recombinant proteins of B. abortus may be potential LPS-free proteins for diagnosis.

• Journal of the Korean association of regional geographers
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• v.13 no.5
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• pp.495-508
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• 2007

This study pays attention to the organization of local-level farming group, which has been recognized as a solution to the problems of agriculture and rural area with their crisis deepened by the coming of FTA. More specifically, this study analyzed each farming household's understanding of the farming group organization of fruit nursery tree growing region, and their participational behavior types. As a result, it is revealed that the organization of farming group oriented to enhance its competence must aim to realize its economic benefits rather than to maintain the social relationship among the members of organization. Also it has to orient itself not to abstract causes but to more concrete and practical objects. In addition, the farming group must be organized based on the identity of growing farm product, and in respect of the reliability among the members, there must be a proper level of control that makes the internal control and the harmonious interaction among the members to be possible.

• The Journal of the Korea Contents Association
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• v.16 no.2
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• pp.399-409
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• 2016

Gyeongsangbuk-do moved its provincial office in October, 2015 and has been building a new town with a goal to construct a truly self-sufficient city with the population of 100,000 people before 2027. However, a new town tends to be dualized in terms of its administrative districts, so it is raising a lot of problems regarding from city construction up to operation. In order to solve those problems, this author has selected Si Gun Gu integration that was chosen as a major task to do in 2012 among the basic plans to revise the local administration system announced by the promotional committee to reorganize administrative districts as the range of this research and analyzed how it is going on in Andong-si and Yecheon-gun where a provincial office new town is being constructed. The main contents of this study regard the theoretical model to reform the local administration system, basic plans to revise the local administration system, and necessity and directions for reorganizing administrative districts in Andong-si and Yecheon-gun. Based on that, this author suggests integration of administrative districts as a way for a provincial office new town to establish its identity and grow as a sustainable, new growth focus city in Gyeongsangbuk-do.

• The Journal of the Korea Contents Association
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• v.17 no.8
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• pp.667-676
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• 2017

The purpose of this study was to examine the experience of practitioners who completed the counseling practice in addiction organizations and to suggest the direction of effective counseling practice for students in interdisciplinary program of addiction majors. For this purpose, we provided the counseling training to two addiction treatment institutions and conducted the interviews with 16 students who participated the counseling practice at the institute and then analyzed the data qualitatively. The results of the study showed that the counseling practitioners experienced fear and prejudice against addicts, prejudice against addicts, change of illness, severity and risk of addiction, career expectation in addiction area, and reality of addiction. Also, the significance of the counseling practice was to give an important lesson in establishing the attitude of self - reflection and the identity of addiction counselor. In the discussion, a systematic management plan of counseling practice in the addiction field was suggested based on the derived topic.

• The Journal of the Korea Contents Association
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• v.17 no.11
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• pp.90-101
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• 2017

This study will analyze that the meaning and the characteristic of Information design type advertising. This study research the advertisement and Information with the issue and explore Information design type advertising samples by doing an in-depth analysis with an expert group and an inexpert group. It attracts customers visualizing sensational information and data as information design technique. It can be classified in to Manual type ad, Identity type ad, Data visualizing type ad. The communication formula of it goes through the keywords: Attention, Curation, Study, and these Curation and Study are new steps which didn't exist before in consumer behavior model. Information used in it comes from common sense or storytelling made by imagination, but there is no example of using false information distorting truth. Not exaggeration and falsehood, interesting which based on confidence creates a bond of sympathy: period time.

• The Journal of the Korea Contents Association
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• v.20 no.5
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• pp.251-260
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• 2020

In , music becomes a passage of spaces in dreams and at the same time causes a problem of temporality between spaces with different time speeds. This paper aims to examine the temporality of the music through Bergson's concept of time. The music used in the film, 《Non, je ne regrette rien》, is divided into the original version, the slowed-down version, and the rearranged version with the slowed-down, and this study visually confirmed the characteristics and similarities through practical analysis. From the perspective of Bergson's perception and memory diagram, non-diegetic music of the actual(the rearranged version) in which diegetic music of the virtual(the slowed-down version) inherent, plays the role of film music and music signal simultaneously. Also, the original version and the slowed-down version are the relationship of durational identity with qualitative changes. We looked at the position in the inverted cone diagram and applied their relationship to the diagram. It is a great achievement of this study that we explored the temporality of music in the multi-layered structure of , based on Bergson's philosophy of coexisting with the present and the past.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.2
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• pp.651-657
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• 2010

Even though local governments are committed to the landscape management aiming to enhance the quality of the urban and natural landscape, the results are not satisfactory since the landscape plan is performed through the "routine" process, on which the planning system is based. Many local governments had spent much in making their landscape plans, but these plans were evaluated not so much "useful" in the practical sense. The objective of the study, which aims to systematically readjust the existing landscape management system of Korea, is to analyse the landscape plans of the few local governments, which are categorized in two level - upper and low local government. The analysis of landscape plan is performed in terms of four factors: The composed structure, the objects of the plan, the effectiveness of the landscapeindicators and guidelines and the institutional system.

• The Journal of the Korea Contents Association
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• v.14 no.12
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• pp.1069-1082
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• 2014

The study aims to gain information for developing educational contents to support LPSMB(low-performing students with multicultural backgrounds) through the survey on their personal characteristics, attitudes related to learning, and learning environment. We analyzed a survey of 2,091 students from 200 schools who were low-performing Korean students, LPSMBs, and normally performing students with multicultural backgrounds. Normally performing students with multicultural backgrounds showed statistically significant higher scores on most questionnaires than low-performing Korean students and LPSMBs. However, normally performing students with multicultural backgrounds and LPSMs showed statistically significant lower scores on identity and social relationships. LPSMBs showed lower scores on parents' culture than normally performing students with multicultural backgrounds. We suggested how to support LPSMBs' learning and learning environment based on the survey results.

• Journal of Korean Library and Information Science Society
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• v.48 no.4
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• pp.1-20
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• 2017

The most important identity of public library is local public goods that guarantee access and use of knowledge and information of local residents. To justify this, the library must create an optimal lighting environment. Particularly, since the perception rate of information through vision is over 80%, it is necessary to secure proper illuminance. This study measured the illuminance level of public libraries in Daegu. Based on the results, this study suggested a desirable illuminance level for each space and proposed plans to secure and maintain proper illuminance(prospective revision of the KS A 3011, setting the illuminance range for each space of the local government, replacing the existing lighting fixtures with LED, enhance supervision of lighting management, establishment of basic principles for proper lighting and detailed plans for each space of the library, investigation and reflection on users' complaints and satisfaction, etc.)

• Nutrition Research and Practice
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• v.1 no.4
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• pp.313-320
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• 2007

This study sought to compare intake levels of major dish items and food groups between normal and obese Korean children in order to understand obesity-related eating behaviors. The study was also conducted to apply the information on the differences in major dish items and food groups found in this study to development of a brief food frequency questionnaire (FFQ) for children's obesity-related eating behaviors. Finally, the developed FFQ was evaluated for reliability. A total of 290, 24-hour dietary recall data from 9-11 year-old normal or obese participants in the 2001 Korean National Health and Nutrition Examination Survey (KNHANES) were reviewed to identity dish items and food groups showing outstanding differences between the normal and obese groups. Based on the level of intake amount between the two groups, a total of 7 items, including ddeokbokggi, spaghetti, ham roast, pork cutlet, dairy products & ices, kimchi, and fruits, were selected to be included in the FFQ. The former 5 items were seen to be consumed more in the obese group, while the latter 2 items were so in the normal group. The questionnaire was formatted into a frequency response section of a seven-category option and reference period of the last 7 days. Test-retest reliability of the developed FFQ was examined by administering it to 153, 9-11 year-old children at a public elementary school in Seoul twice at a month interval. The level of reliability was found to be reasonably high. In conclusion, this study suggests that high consumption of several high-calorie dish items and low consumption of kimchi and fruits may be important eating behaviors in relation to obesity risk among 9-11 year-old children who need nutrition educators' attention. The current study also implies that a simple FFQ can be utilized to reliably assess 9-11 year-old children's diets.