• 한국식품과학회지
• /
• 제39권6호
• /
• pp.721-724
• /
• 2007

신선초의 건조분말을 사료에 5%수준으로 혼합하여 백서에 8주간 투여하였을 때, 총 cholesterol, LDL-cholesterol, VLDL-cholesterol의 농도를 낮추며, HDL-cholesterol의 농도는 높이는 효과가 있음이 관찰되었다. 신선초에 존재한다고 알려진 항산화성 flavonoid들 중 quercetin과 그 배당체의 하나인 isoquercitrin이 백서의 체내에서 측정된 반면, 다른 quercetin 배당체인 hyperoside는 측정되지 않았다. 이 결과는 장기간 신선초투여에 의해 신선초에 함유된 quercetin 또는 그 배당체가 실제로 체내로 흡수되어 약리작용을 발휘할 수 있을 가능성을 제시해 준다.

• 한국식생활문화학회지
• /
• 제34권1호
• /
• pp.75-83
• /
• 2019

This study was conducted to investigate the fermentation characteristics and anti-obesity effects of Cheonnyuncho (Oputia Humifusa) fruit fermented with Lactobacillus plantarum SRCM 100320 (FC). The pH gradually decreased from 4.77 to 3.63 at 72 hours during fermentation. Counts of lactic acid bacteria, total polyphenol and flavonoid contents and DPPH scavenging activity were the highest at 48 hours during fermentation. Evaluation of the composition of polyphenols and flavonoids of FC fermented at 48 hours by HPLC revealed hyperoside (quercetin 3-galactoside), luteolin and kaempferol were the major components. The hyperoside content of FC was decreased, while the luteolin and kaempferol contents of FC were increased compared to unfermented Cheonnyuncho (NFC). Evaluation of the anti-obesity effects of FC in 3T3L-1 cells revealed that the accumulation of triglyceride was inhibited by about 27.3% in cells treated with FC at $150{\mu}g/mL$ compared to NFC. These findings indicate FC has the potential for use as an anti-obesity material.

• 한국식품영양과학회지
• /
• 제26권2호
• /
• pp.236-241
• /
• 1997

일부 식용식물 추출물의 HMG-CoA reductase 저해 활성을 탐색한 결과 파, 마늘, 호박의 물 추출물에서 $10{\sim}14%$의 저해활성이 관찰되었고 그외 부추, 생강 등의 물 추출물도 약간의 저해활성을 보였다. Methanol 추출물의 경우는 참취, 마늘, 생강, 미나리, 신선초 등에서 $51{\sim}29%$의 강한 저해활성이 관찰되었고, 그 외 느타리, 호박, 양송이, 고추, 토란줄기, 부추, 당근 등에서도 $12{\sim}3%$의 저해활성이 나타났다. 저해활성이 비교적 높은 신선초의 methanol 추출물을 극성의 차이에 따라 chloroform, ethyl acetate, n-butanol, 물층의 순서로 분획하여 각 분획별로 HMG-CoA reductase 저해활성을 측정한 결과 ethyl acetate 분획에서 가장 높은 저해 활성이 관찰되었으며 n-butanol, chloroform, 물층에서도 비교적 높은 저해 활성이 보였다. 신선초의 methanol 추출물 분획 중 HMG-CoA reductase 저해 활성이 가장 높게 나타난 ethyl acetate 분획을 silica gel column chromatography를 이용하여 hyperoside와 luteolin $7-O-{\beta}-D-glucoside$를 순수 분리하여 HMG-CoA reductase 저해활성을 측정한 결과 luteolin-7-O-glucoside는 $30{\mu}M$ 농도에서 65.5%의 강한 저해활성을 보였고 hyperoside는 14.8%의 비교적 약한 저해활성을 나타내었다.

• 생약학회지
• /
• 제25권3호
• /
• pp.199-203
• /
• 1994

Chemical examination of the leaves of Betula platyphylla var. latifolia has led to the isolation and characterization of five flavonoid glycosides including two C-glucosyl flavonoids. The structures of these compounds were elucidated as myricetin $3-O-{\alpha}-_L-rhamnoside$ (myricitrin), $quercetin-3-O-{\beta}-_D-glucopyranoside$ (isoquercitrin), $quercetin-3-O-{\beta}-_D-glucopyranoside$ (hyperoside), $nalingenin-6-C-{\beta}-_D-glucopyranoside$ (hemiphloin) and $aromadendrin-6-C-{\beta}-_D-glucopyranosidre(6-C-glucosyldihydrokaempferol)$ on the basis of physico-chemical and spectroscopic evidences.

• Natural Product Sciences
• /
• 제7권1호
• /
• pp.23-26
• /
• 2001

Five flavonoids and one aromatic acid were isolated from the leaves of Zanthoxylum piperitum. The structures of compounds were elucidated as quercetin, afzelin, quercitrin, hyperoside, hesperidin and protocatechuic acid on the basis of spectral evidence.

• 생약학회지
• /
• 제39권2호
• /
• pp.146-149
• /
• 2008

From the leaves of Angelica keiskei (Umbelliferae), luteolin, protocatechuic acid, guaijaverin, hyperoside and cynaroside were isolated and characterized by spectral data. Luteolin and protocatechuic acid showed potent DPPH radical scavenging activity.

• Natural Product Sciences
• /
• 제17권4호
• /
• pp.342-349
• /
• 2011

This study was performed to determine the composition of phenolic substances contained in the leaves of Cirsium setidens (Compositae), validate the high-performance liquid chromatography (HPLC) method, and determine the in vivo sedative effect of the main component pectolinarin. Six phenolic compounds isolated from C. setidens were spectroscopically identified as chlorogenic acid (1), hyperoside (2), 3,4-di-O-caffeoylquinic acid (3), caffeic acid methyl ester (4), linarin (5), and pectolinarin (6) and then used as standard compounds for HPLC analysis. HPLC proved to be precise, accurate, and sensitive for the simultaneous analysis of the phenolic substances. In particular, six compounds showed good regression ($R^2$ > 0.999) within test ranges and recovery was in the range of 95.4 - 104.8%. The content of pectolinarin was considerably higher (156.48 mg/g) than those of other phenolic substances including the other flavone glycoside, linarin (18.99 mg/g). The contents of other phenolic substances, in order, were chlorogenic acid (8.41 mg/g), 3,4-di-O-caffeoylquinic acid (5.74 mg/g), hyperoside (4.33 mg/g), and caffeic acid methyl ester (0.51 mg/g). Oral administration with compound 6 (10 and 20 mg/kg) enhanced the sleeping time induced by pentobarbital in mice, indicating that it has a sedative effect.

• 임산에너지
• /
• 24권2호
• /
• pp.16-23
• /
• 2005

삼지구엽초의 각 부분(잎, 줄기, 뿌리)을 MeOH로 추출한 후 농축하고 핵산, 클로로포름, 부탄올, 에틸아세테이트 및 수용성으로 분획하여 동결건조하였다. 각 추출물은 메탄을 수용액 및 에탄올-핵산 혼합액을 사용하여 Sephadex LH-20 칼럼크로마토그래피로 화합물을 분리 한 후 TBA 및 6% 초산에 전개하는 셀룰로오스 박층 크로마토그래피로 단리물질을 확인하였다. 단리물질의 화학적 구조는 $^1H-NMR,\;^{13}C-NMR$ 및 질량 분석으로 결정하였으며 단일화합물로 정제된 물질은 caffeic acid를 비롯하여 flavonoid와 그 배당체 화합물인 icariin, caffeic acid, hyperoside, Ikarisoside A, (+)-catechin이었다. 또한, 각 부분의 클로로포름 분획을 GC/MS (Libraries search)를 이용하여 정성분석을 실시하였다.

• Toxicological Research
• /
• 제33권4호
• /
• pp.283-290
• /
• 2017

The host immune system is the first line of host defense, consisting mainly of innate and adaptive immunity. Immunity must be maintained, orchestrated, and harmonized, since overactivation of immune responses can lead to inflammation and autoimmune diseases, while immune deficiency can lead to infectious diseases. We investigated the regulation of innate and adaptive immune cell activation by Artemisia capillaris and its components (ursolic acid, hyperoside, scopoletin, and scopolin). Macrophage phagocytic activity was determined using fluorescently labeled Escherichia coli, as an indicator of innate immune activation. Concanavalin A (ConA)- and lipopolysaccharide (LPS)-induced splenocyte proliferation was analyzed as surrogate markers for cellular and humoral adaptive immunity, respectively. Neither A. capillaris water extract (WAC) nor ethanol extract (EAC) greatly inhibited macrophage phagocytic activity. In contrast, WAC suppressed ConA- and LPS-induced proliferation of primary mouse splenocytes in a dose-dependent manner. Similarly, EAC inhibited ConA- and LPS-induced splenocyte proliferation. Oral administration of WAC in mice decreased ConA- and LPS-induced splenocyte proliferation, while that of EAC suppressed LPS-induced splenocyte proliferation. Repeated administration of WAC in mice inhibited ConA- and LPS-induced splenocyte proliferation. Ursolic acid, scopoletin, and scopolin reduced ConA- and LPS-induced primary mouse splenocyte proliferation, while hyperoside did not show such activity. These results indicate that A. capillaris and its components, ursolic acid, scopoletin, and scopolin, suppress ConA- and LPS-induced adaptive immune cell activation. The results suggest that A. capillaris is useful as a regulator of adaptive immunity for diseases involving excessive immune response activation.

• 한국식품영양과학회지
• /
• 제25권4호
• /
• pp.588-592
• /
• 1996

미나리의 MeOH추출물에서 분획한 ethyl acetate 가용부를 silica gel column chromatography를 이용하여 isorhamnetin, hyperoside 및 persicarin의 플라보노이드 화합물을 분리하였으며, in vitro에서 Salmonella typhimurin TA 100균주를 이용하여 항돌연변이 활성을 관찰하였다. 이들 화합물 중 isorhamnetin은 0.5mg/plate에서 33%, 1.0mg/plate에서는 59%, persicarin은 0.5mg/plate에서 40%, 1.0mg/plate에서 47%의 돌연변이 저해효과를 나타내었다. 이와 유사한 플라보노이드 화합물로서 엉겅퀴 지상부에서 분리한 acacetin 7-rutinoside, cirsimaritin 4＇-glucoside 및 hispidulin 7-neohesperidoside에 대해서도 1mg/plate 농도에서 acacetin 7-rutinoside와 cirsimaritin 4＇-glucoside가 40% 및 49%의 돌연변이 억제활성이 관찰되었다.