• Journal of Nutrition and Health
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• 제34권2호
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• pp.141-149
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• 2001

This study done to investigate the hypocholesterolemic effect of ow dietary fiber in a high cholesterol diet in aspect of cholesterol absorption and excretion using Sprague Dawley rats. After feeding diets containing 0.5% cholesterol and 5% tangerine pulp, sea tangle or prickly pear cactus for four weeks, we measured the levels of plasma and liver cholesterol and triglyceride, fecal neutral sterols and fecal radioactivity after ingestion 14(sup)C-cholesterol. We also examined platelet aggregation and histological change in liver tissues in association of hypercholesterolemia. The liver to body weight ratio was significantly(p<0.01) lower in rats fed prickly pear cactus than in other groups. The levels of plasma total cholesterol, LDL-cholesterol and triglyceride were decreased significantly(p<0.01) in the prickly pear cactus group compared with the control, while there was no difference in the liver levels of total cholesterol and triglyceride among groups. Fecal corprostanol and cholesterol were significantly(p<0.01) higher groups of control and prickly pear cactus compared to the other two groups. Radioisotope excretion after ingestion of 14(sup)C-cholesterol was higher in the control group than in tangerine pulp group or sea tangle group, with the highest in prickly pear group. Radioisotope excretion was the highest during the 2nd and 3rd days in all groups. Hematocrit and platelet aggregation were decreased in all fiber groups compared to the control, but not statistically different. Microscopic examination showed that cholesterol diet cause a fat accumulation in the liver and prickly pear cactus decreased the fat accumulation. Result indicates that prickly pear cactus has hypocholesterolemic effect by decreasing absorption and increasing excretion of cholesterol, thereby protective effect on fatty liver. Control group fed diet containing high cholesterol and low fiber seems to have a self control system in cholesterol absorption and excretion preventing hypercholesterolemia. (Korean J Nutrition 34(2) : 141-149, 2001)

• Journal of Nutrition and Health
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• 제32권5호
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• pp.552-560
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• 1999

In this study, we investigated the effects of lowering the fat and cholesterol in the diets of 26 Korean hypercholesteolemic men($\geq$240mg/dl). They consumed 2378kcal/day with 20.9% of the energy deriving from fat, and a cholesterol intake of 282mg(118mg/1000kcal). The experimental diet consisted of 2400kcal, the same as their usual diet, but the fat content was restricted to 15%, and the cholesterol level to below 100mg/1000kcal. The subjects kept to this diet for four weeks and were asked to maintain their usual life activities during the experimental period. The dietary intake and levels of plasma lipid, lipoprotein-cholesterol and apoprotein of the subjects were analyzed before, two weeks into, and after four weeks dietary intervention. After two of the dietary intervention, there were no significant changes of plasma total cholesterol or triglyceride levels but there was some changes of phospholipid level. However, after four weeks, the levels of plasma total cholesterol, triglyceride, and phospholipid had decreased significantly: 18.2%, 32.9%, and 11.9%, respectively. And the LDL-cholesterol and VLDL-cholesterol levels also showed a marked reduction of 18.1%, and 33.0% respectively without change of HDL-cholesterol level. There were no changes in the levels of Apo-A 1, Apo-B, or Lp(a). The changes of the plasma lipid levels were significantly associated with the changes in dietary fat intake but not the cholesterol intake. In conclusion, although the responses to the dietary intervention varied among the individual subjects, the lowering of dietary fat component from 21% to 15% of energy intake seems to be an effective way to reduce plasma cholesterol and triglyceride levels without decreasing HDL-cholesterol level. It was also found that the restriction of dietary cholesterol to below 100mg/1000kcal not seem to be effective for the hypercholesterolemic patient who already consuming below 300mg/day of cholesterol.

• Journal of Chest Surgery
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• 제41권6호
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• pp.687-694
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• 2008

배경: 대동맥 판막 경화증은 고령에서 흔히 나타나며, 대동맥 판막 협착증처럼 좌심실 유출로를 폐쇄하지는 않으나 심혈관질환으로의 사망 위험성이 50%나 증가되며, 심근경색증의 위험성이 높다. 그러나, 대동맥 판막경화증에서 ${\beta}_3$ integrin의 관련성은 잘 알려져 있지 않다. 대상 및 방법: 20마리의 뉴질랜드산 토끼들을 2군으로 나눈 후, 1군(10마리)에서는 정상식이를 시행하였고, 2군(10마리)에서는 1% 콜레스테롤식이를 시행하였다. 12주간 식이후 실험동물을 희생시켜 대동맥 판막 및 상행대동맥을 채취하였다. 각군의 토끼들의 혈장에서 총 콜레스테롤, 중성지방, 저밀도(LDL)-콜레스테롤, 고밀도(HDL)-콜레스테롤 수치를 측정하였으며, 대동맥 판막과 대동맥에서 HE 염색을 시행하였고, 대동맥 판막에서 근육섬유모세포, 대식세포에 대한 면역조직화학염색을 시행하였으며, ${\beta}_3$, integrin에 대한 정량적검사를 위하여 Real-time polymerase chain reaction (RT-PCR)을 시행하였다. 결과: 총 콜레스테롤($2148.3{\pm}1012.5\;mg/dL$ versus $53.7{\pm}31.8\;mg/dL$, p<0.05), 중성지방($240.4{\pm}218.3\;mg/dL$ versus $31.6{\pm}6.4\;mg/dL$, p<0.05), 저밀도-콜레스테롤 수치($2,065.3{\pm}960.9\;mg/dL$ versus $29.1{\pm}30.9\;mg/dL$, p<0.05)는 정상식이군과 비교해서 콜레스테롤 식이군에서 의미 있게 증가하였다. 대동맥판막의 면역 조직화학염색에서 근육섬유모세포와 대식세포는 콜레스테롤식이군에서 발현이 증가되었다. RT-PCR을 이용한 정량적 검사에서 ${\beta}_3$ integrin mRNA의 발현은 대동맥판막과 대동맥 모두 콜레스테롤 식이군에서 의미 있게 감소되어 있었다(p<0.05). 결론 : 콜레스테롤식이에 의한 고콜레스테롤혈증은 토끼의 대동맥 판막에서 대동맥 판막경화증을 유발하였다. 석회화 대동맥 판막의 초기 상태인대동맥 판막 경화증은 초기죽상경화병변과 유사한 과정을 보이며, 이는 ${\beta}_3$, integrin의 감소가 중요한 역할을 한다고 생각된다.

• 한국식품영양과학회지
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• 제15권1호
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• pp.75-81
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• 1986

w-3계 linolenic acid가 풍부한 들깨기름 및 EPA와 DHA가 함유된 말쥐치기름의 혈청 및 간장의 지질성분에 미치는 영향을 구명하기 위해 지방산조성이 다른 몇가지 유지를 고콜레스테롤 식이 흰쥐에 식이하므로서 혈청 및 간장중의 지질농도에 미치는 영향을 비교 실험하여 다음과 같은 결과를 얻었다. 1. 사료섭취량과 증체량은 전 시험군간에 있어 별다른 차이가 없었다. 2. 혈청의총 cholesterol농도는 soybean oil perilla oil및 file fish oil 식이군이 대조군에 비해 유의적으로 낮았다. HDL-cholesterol 농도는 file fish oil 식이군이 가장 높았으며, perilla oil 식이군도 약간의 상승효과가 있었다. 총 cholesterol에 대한 HDL-cholesterol의 비율은 file fish oil식이군이 가장 높았다. 3. 간장의 총 cholesterol농도는 전 시험군간에 유의적인 차이는 없으나 file fish oil 및 perilla oil 식이군에서 약간 낮은 경향이었다. 4. 혈청의 triglyceride농도는 soybean oil 및 perilla oil식이군이 대조군에 비해 유의적으로 낮았다. phospholipid의 농도는 file fish oil식이군이 가장 낮았으며, soybean oil 및 perilla oil식이군에서도 대조군에 비해 유의적으로 낮았다. 5. 간장중의 triglyceride및 phospholipid의 농도는 전시험군간에 유의적인 차이는 없으나, perilla oil식이군이 약간 낮은 경향이었다. 이상의 결과로 미루어 보아 perilla oil 및 file fish oil은 혈청의 총 cholesterol, 및 phospholipid의 농도저하 효과가 다소 있는 것으로 사려된다.

• 한국식품영양과학회지
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• 제37권10호
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• pp.1244-1250
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• 2008

본 연구는 양파 첨가 청국장 첨가 식이가 고 콜레스테롤 흰 쥐의 지질대사 및 항산화대사에 미치는 영향을 알아보기 위해 Sprague-Dawley 수컷 32마리를 대조군(C), 고 콜레스테롤군(HC), 고콜레스테롤＋양파 첨가 청국장군(HC-OC), 고콜레스테롤＋청국장군(HC-C)로 나누어 실험 식이를 8주간 공급하였다. 8주 후 혈액과 간, 변을 수집하여 혈장, 간 및 변의 지질농도, 혈장 TRAP, CD, 적혈구내 항산화효소활성, comet assay를 통한 DNA 손상을 측정하였다. 모든 군에서 식이섭취량은 유의적 차이가 없었고, HC군에서 체중이 증가하였으며, 그 중 HC-C군에서 체중과 식이효율이 유의적으로 높았다. 간 무게는 C보다 고콜레스테롤 급여 군들에서 유의적으로 증가하였고, 분변량은 고콜레스테롤 식이군들에서 낮았으며, 변의 조지방은 고콜레스테롤 식이 군들에서 증가하였다. 양파 첨가 청국장과 청국장 급여로 HC군보다 간의 총 콜레스테롤 함량은 유의적으로 낮아졌으며, 혈장 지질 저하효과는 나타나지 않았다. 양파 첨가 청국장과 청국장 섭취로 부분적 간 기능 개선 효과를 나타내었으며, 청국장 급여로 혈장 CD농도는 유의적으로 낮아졌다. 그리고 HC-OC군과 HC-C군의 tail intensity는 감소하여 DNA 산화적 손상에 대한 보호 효과를 나타내었다. 따라서 양파첨가 청국장과 청국장은 부분적으로 지질 개선효과와 항산화력이 있음을 확인할 수 있었다. 그러나 양파와 청국장은 각각이 이미 우수한 항산화력을 가지고 있는 기능성식품으로서 이 두 소재의 혼합에 의한 상승효과는 크게 나타나지 않았던 것으로 생각된다.

• Journal of Nutrition and Health
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• 제56권4호
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• pp.361-376
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• 2023

본 연구에서는 햄프씨드 오일이 고콜레스테롤혈증을 개선시키는 지 확인하기 위하여 Sprague-Dawley rat에 1.25% 콜레스테롤식이를 8주 동안 급여하였으며, 햄프씨드 오일을 두 농도 (저농도, 고농도)로 경구 투여한 후 혈액, 조직 분석을 통하여 지질개선 및 염증 완화 효과를 발휘하는지 분석하였다. 햄프씨드 오일 식이는AMPK/SREBP-2 경로를 경유하여 혈중 콜레스테롤의 합성을 효과적으로 억제하였으며, PI3IK/Akt/NF-κB의 경로를 유의하게 억제하여 항염증 효과를 발휘함을 알 수 있었다. 이러한 신호전달의 경로는 간 조직에서 지질축적의 감소와도 관련이 있는 것으로 보인다. 이상의 결과를 종합해보면 햄프씨드 오일은 고콜레스테롤혈증의 예방 및 치료를 위한 잠재적 후보소재로서 가능성이 있음을 시사한다.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1230-1235
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• 1998

For the investigation to the effects of H. cordata on prevention of hypercholesteromia, dietary hypercholesteromic rats were fed for 4 weeks with basial diet containing 5, 10, 15, 20% H. cordata powder, 5, 10 and 15% H. cordata juice. Total cholesterol and triglyceride contents of livers were significantly lower in experimental groups than in control group. Phospholipid contents in livers were not significant difference between control and experimental groups. Contents of free cholesterol in livers were ranged from 0.4$\pm$0.2 to 0.7$\pm$0.5mg/kg and cholesteryl ester contents were lower in H. cordata juice added groups than H. cordata powder added groups. Total cholesterol content in brains were not significant difference between cholesterol not added, H. cordata powder added at 15, 20% and its juice added group at 15%. The more powder and juice of H. cordata were added to basial diets, the lower contents of triglyceride and phospholipid were detected in brains. Contents of free cholesterol and cholesteryl ester in brains were not significance in all experimental groups. In kidneys, total cholesterol contents were not significant in control group and H. cordata powder of 5% was added group. Triglyceride, phospholipid and cholesteryl ester contents in kidney were not significant in all groups. Contents of free cholesterol in kidney were equally significant between cholesterol free and 15% H. cordata juice treated group.

• 환경생물
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• 제28권2호
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• pp.79-85
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• 2010

To examine the effect of Codium fragile on blood cholesterol and lipid metabolism, hyperlipidemia was induced in experimental animal rats through the administration of a hypercholesterolemic diet. Codium fragile powder was then administered to the rats for 5 weeks, after which, blood biochemical changes such as blood cholesterol, Aspartate Aminotransferase (AST: serum SGOT) and Alanine Aminotransferase (ALT: serum SGPT) enzyme activity, etc. were determined. And histological changes in liver cells were examined using an electron microscope. Codium fragile treatment resulted in a significant reduction of the levels of total cholesterol, blood triglyceride and low-density cholesterol (LDL. Chol) compared to the control rats. In contrast the expression levels of high-density cholesterol (HDL. chol.) were increased. The AST value of the Codium fragile administration group was significantly reduced and the blood ALT value of the Codium fragile group showed a significant decrease in comparison to the negative control group. In summary, this study demonstrated the beneficial possibilities of Codium fragile in improving the abnormality of lipid metabolism caused by liver cell damage and hyperlipidemia.

• 대한한방내과학회지
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• 제35권3호
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• pp.244-261
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• 2014

Objectives: This experiments were performed to determine the effects of the Jehasuogamibang (製何首烏加味方: JHGB) on antioxidationactivity and hyperlipidemia induced by a hypercholesterolemic diet in mice. Methods: After treatment with JHGB expert safety in cytotoxicity and toxicity of Human fibroblast cells and liver and kidney, effect on Reactive Oxygen Species, serum total cholesterol, LDL-cholesterol, triglyceride, glucose, HDL-cholesterol, lipid peroxid of liver tissue, significantly increased SOD and catalase. Results: 1. JHGB showed safety in cytotoxicity and toxicity of human fibroblast cells and liver and kidney. 2. JHGB showed significant inhibitory effect on reactive oxygen species. 3. JHGB significantly decreased serum total cholesterol, LDL-cholesterol, triglyceride, and glucose, and significantly increased serum HDL-cholesterol. 4. JHGB significantly decreased lipid peroxide of liver tissue and significantly increased SOD and catalase. Conclusions: These results suggest that Jehasuogamibang is effective in antioxidationactivity and dietary hyperlipidemia-induced mice.

• 대한가정학회지
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• 제30권2호
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• pp.61-71
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• 1992

This study was designed to evaluate the anti hypercholesterolemic effect in either soybean oil or lard containing hypercholesterol diet in rats. Rats were fed, ad libitum, for 5 weeks with diets containing 0, 5, 10% of L.edodes in either soybean oil or lard with 1% exogenous cholesterol. Forty-two male sprague-dawley rats weighing 71.6$\pm$8.1g were divided into 6 groups. The results of this study were summairzed as follows: 1. Weight gain, total feed intake, feed intake efficiency ratio, total calorie intake and calorie efficiency ratio during five weeks showed significant difference among groups. The lowest value was found in soybean oil ＋ L.edodes-free group and the highest value was found in lard ＋ L.edodes-free group. The weight of organs were significantly higher in lard groups than soybean oil groups. 2. The contents of serum triglyceride showed significant difference kinds of lipids. It tended to be lower soybean oil groups than lard groups. It was difference with dietary L.edodes levels in soybean oil groups. The contents of total serum cholesterol showed significant difference by kinds of lipids, it was lower soybean oil groups than lard groups. But it was not differ with dietary L.edodes levels in soybean oil groups. 3. The contents of total liver cholesterol showed no difference kinds of lipids. And it was lower in soybean oil groups than lard groups. This result showed that the soybean oil decreased the contents of total liver cholesterol.