• Title/Summary/Keyword: hydroxy radical scavenging

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Quality Characteristics of New Reddish Brown Color Rice Variety "Superhongmi" (슈퍼홍미 벼품종의 품질특성)

  • Ryu, Su-Noh
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.436-441
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    • 2015
  • 'Superhongmi' is a new rice variety with reddish brown color developed from a crossing a high cyanidin 3-glucoside (C3G) content beeeds including Heugjinju and Suwon 425 and a big grain size breed such as Daeribbyeo. This variety heads on Sep. 5 and has 86.7 cm culm length. The fertility ratio of Superhongmi was about 81.7% and weight of 1,000-grain was about 26.8 g. The 1,000 grain weight of Superhongmi was heavier than that of Superjami. The total essential amino acid content of Superhongmi was lower than those of others. However, Superhongmi was high in tyrosine and arginine. Total polyphenol content of Superhongmi was 2 times higher than that of Heugjinju and flavonoid content was 8 times higher than that of Dongjinbyeo. In addition, hydroxy radical scavenging activity of Superhongmi was significantly higher than that of Dongjinbyeo. However, significant differences were not found in the newly breed colored rice. These results suggest that Superhongmi variety has very high value as a source of various functional food as well as stable food.

Effects of Chilbokyeum(七福飮) on the Biochemical Changes in Brain Tissue of Senile Rats (칠복음(七福飮)이 노화(老化) 백서(白鼠) 뇌조직(腦組織)의 생화학적(生化學的) 변화(變化)에 미치는 영향(影響))

  • Son Jeong-Seok;Lyu Young-Su
    • Journal of Oriental Neuropsychiatry
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    • v.8 no.2
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    • pp.25-37
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    • 1997
  • The present experiment was designed to examine catecholamines, 5-hydroxytryptamine, amino acids. malondialdehyde(MDA) and free radical scavenging activity, by administering Chilbokyeum extracts of a variety of concentration to senile brain rats. The results were summarized as followings ; 1. Chilbokyeum significantly increased noradrenalin in the hippocampus and hypothalamus of the brain tissue of senile rats, ad even though Chilbokyeum increased noradrenalin also in other brain tissue, there was no significance. 2. Chilbokyeum had no effects on dopamine changes in all brain tissue of senile rats.3. Chilbokyeum significantly increased 5-hydroxy-tryptamine in cerebellum, but decreased in other brain tissue.4. Chilbokyeum increased amino in the brain tissue of senile rats. 5. Chilbokyeum significantly decreased MDA and free radical in the brain tissue of senile rats. According to the above results, Chilbokyeum is assumed to improve brain function by reaction by reacting on biochemical of the senile brain, and that Chilbokyeum can be used to treat regressive brain disease carrying symptoms of psychoactive disorders.

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Antioxidant, Anticancer and Immune Activation of Anthocyanin Fraction from Rubus coreanus Miquel fruits (Bokbunja) (복분자 안토시아닌 분획의 항산화, 항암 및 면역증진 효과)

  • Choung, Myoung-Gun;Lim, Jung-Dae
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.4
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    • pp.259-269
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    • 2012
  • This study was performed to determined the antioxidant activities, anticancer and immuno-activities of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Anthocyanin fraction extracted from Bokbunja revealed the presence of three anthocyanin components, which were tentatively identified as cyanidin 3-O-sambubioside, cyanidin 3-O-xylosylrutinoside and cyanidin 3-O-rutinoside using RP-HPLC/DAD/MS. The anthocyanin fraction from Bokbunja always showed reducing power and high scavenging activities against DPPH, hydroxy radical (OH) and superoxide anion radical ($O_2{^-}$) similar to general synthetic antioxidant and polyphenol compounds from plant origin. Anthocyanin fraction from Bokbunja showed high inhibition on proliferation of LNCaP and A549 cells and did not inhibit the proliferation of other cancer cells. Immuno-activities of Anthocyanin fraction from Bokbunja were investigated, it showed high promotion of human B and T cells growth about 50% and secretion of IL-6 and TNF-${\alpha}$ by treatment after 6 days. Over all, the result of the study suggest that anthocyanin fraction from Bokbunja displays antioxidant activity comparable to that general synthetic antioxidant, also, anthocyanin fraction from Bokbunja are expected to be good candidate for development into source of anticaner and immuno-activator agent in food industry.

Antioxidant Activities of Acidic Ethanol Extract and the Anthocyanin Rich Fraction from Aronia melanocarpa (아로니아 추출물 및 아로니아 안토시아닌분획의 항산화 활성 효과)

  • Lim, Jung-Dae;Cha, Hwan-Soo;Choung, Myoung-Gun;Choi, Ri-Na;Choi, Duck-Joo;Youn, Aye-Ree
    • Korean journal of food and cookery science
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    • v.30 no.5
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    • pp.573-578
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    • 2014
  • The objective of this study was to investigate the antioxidant activities and anthocyanin profiles in the anthocyanin rich fraction (ARFAM) of Aronia melanocarpa, which are considered functional substances and are available as food coloring agents in Korea. Anthocyanins were identified by reversed-phase C18 column chromatography and HPLC-DAD-ESI/MS analysis. The antioxidative activity of the acidic ethanol extract (AME) and the anthocyanin-rich fraction (ARFAM) was determined by scavenging of the diphenylpicrylhydrazyl (DPPH) radical, the hydroxy radical, and the superoxide anion in addition to reducing power using a commercial antioxidant as a reference.

The anti-oxidant effects of Ligusticum chuanxiong, Cnidium officinale and their mixture with Angelica gigas (토천궁(土川芎)과 일천궁(日川芎) 및 당귀배합방(當歸配合方)의 항산화(抗酸化) 효과(效果)에 관(關)한 연구(硏究))

  • Park, Yong-Ki
    • The Korea Journal of Herbology
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    • v.22 no.4
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    • pp.101-108
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    • 2007
  • Objectives : To compare the anti-oxidant effects of Ligusticum chuanxiang and Cnidium officinale extracts and their mixture with Angelica gigas, we investigated the anti-oxidative activities using rat liver tissues. Methods : We investigated the anti-oxidative activities by analysis of lipid peroxidation, xanthine oxidase activity, aldehyde oxidase activity, hydroxyl radical scavenging activity, superoxide dismutase activity and catalase activity in rat liver tissues. Results : Both Ligusticum chuanxing and Cnidium offieinale inhibited the lipid peroxidation compared to the control, there is no significant differences between two groups. Cnidium officinale showed strong inhibitory effects on xanthine oxidase and aldehyde oxidase activities compared with that of Ligusticum chuanxing. Ligusticum chuanxing and Cnidium officinale were scavenged the hydroxy radicals, and increased SOD and catalase activities. These results suggested that Ligusticum chuanxing and Cnidium officinale were showed antioxidant activity, especially Cnidium officinale showed higher activity than Ligusticum chuanxing. Conclusions: These results suggest that Ligusticum chuanxing could be replaced by Cmdium afficinale in clinical trial.

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Preparation and Availability Analysis of Vegetable Glycoprotein (식물성 당단백질의 제조 및 유효성 분석)

  • Lee, Mi-Jin;Jeong, Noh-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.26 no.3
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    • pp.248-262
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    • 2009
  • This study is on the feasibility of use of glycoprotein in various areas such as cosmetics and food etc. by extracting, isolating and refining glycoprotein from carrots, red ginseng extract residue, sesame and pine needles using protease(pepsin) and by analyzing general characteristics and measuring various bioactivities. The results of analysis of nutritional composition showed protein contents of glycoprotein. In the analysis of constitutive amino acids, the ratio of contents of hydroxy proline and glycine, the characteristics of glycoproteins appeared similar and the contents of glutamic acid and aspartic acid appeared higher. As a result of measurement contents of total polyphenol and flavonoid, it showed that glycoprotein had more contents generally, and the effect of bioactivity of glycoprotein appeared higher although different kinds of glycoprotein showed a little DPPH radical and nitrite scavenging ability, total antioxidant capacity by ABTS, ACE inhibitory.

Study on Cosmeceutical Acitivies of Natural Material (천연소재의 화장품약리활성에 관한 연구)

  • Kim, Young-Hun;Cho, Woo-A;Lee, Jin-Tae
    • Journal of the Korean Society of Fashion and Beauty
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    • v.5 no.3
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    • pp.99-104
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    • 2007
  • The most extensively used synthetic antioxidants are butylated hydroxyanisole (BHA), butylated hydroxy toluene (BHT) and tert-butylhydroquinone (TBHQ). However BHT and BRA have been suspented of being responsible for liver damage andcar cinogenesis. Therefore, the importance of the search and exploitation of natural antioxidant, especially of plant origin, has greatly increased in recent years. Plant contain a wide variety of chemicals that have potent biological effects. As a result, there has been a growing interest in the use of herbs as a source of therapeutic drugs. The aim of this study was to assess the antioxidant and cosmeceutical of natural materials. The antioxidant and cosmeceutical activity of natural materials were investigated by hydroxyl radical scavenging, superoxide dismutase (SOD) -like, xanthine oxidase inhibition, tyrosinase inhibition, anti-microbial and astringent.

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Antioxidative Activity of Jeolpyeon Containing Lotus (Nelumbo nucifera Gaertn) Seed Powder (연자육 분말 첨가 절편의 항산화 활성)

  • Jeong, Se-Hyun;Kim, Sun-Im;Sim, Ki-Hyeun
    • Journal of the Korean Society of Food Culture
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    • v.27 no.5
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    • pp.505-511
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    • 2012
  • This study was conducted to investigate the antioxidant activity of Jeolpyeon containing lotus seed powder. Original Jeolpyeon and Jeolpyeon with BHT (butyl hydroxy toluene) werecompared. Antioxidant activities were measured based ontotal polyphenolic contents, total flavonoid contents, DPPH radicalscavenging activity, reducing power, and SOD-like activity. Total polyphenolic and total flavonoid contents of Jeolpyeon containing lotus seed powder were estimated at $37.81{\pm}0.45$ mg GAE/g and $26.35{\pm}0.13$ mg QE/g respectively. DPPH radical scavenging activity increased upon addition of lotus seed powder. Further, reducing power was higher in Jeolpyeon containing lotus seed powder than original Jeolpyeon or Jeolpyeon with BHT. The antioxidative effect of Jeolpyeon significantly increased uponaddition of lotus seed powder.

The Antioxidant Effect of Hot Water Extract from the Dried Radish (Raphanus sativus L.) with Pressurized Roasting (가압볶음 무말랭이 열수 추출물의 항산화 효과)

  • Song, Yeong-Bok;Choi, Jeong-Sun;Lee, Ji-Eun;Noh, Jeong-Sook;Kim, Mi-Jeong;Cho, Eun-Ju;Song, Yeong-Ok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.8
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    • pp.1179-1186
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    • 2010
  • The antiradical property of hot water extract from dried radish (DR) or dried radish roasted with pressure (DRRP) was investigated in vitro and in LLC-PK1 cell system. The contents of total free amino acid and reducing sugar in DR were decreased by 72.86% and 3.17%, respectively, after pressurized roasting. In vitro test, $IC_{50}$ for DR and DRRP for DPPH radical scavenging activity were 646.70 and $135.45\;{\mu}g/mL$, 896.10 and $566.98\;{\mu}g/mL$ for superoxide anion radical, and 722.26 and $531.84\;{\mu}g/mL$ for hydroxy radical, respectively. The radical scavenging effects of DRRP was significantly greater than those for DR (p<0.001). These radical scavenging effects of DR and DRRP were confirmed in LLC-$PK_1$ at which oxidative stresses were induced by superoxide, nitric oxide and peroxynitrite generated in the treatment of pyrogallol, SNP, and SIN-1, respectively. Cell viability was increased in the presence of DR or DRRP, dose dependently (p<0.05), and TBARS formation was decreased. The protective effects of DRRP against oxidative damage in LLC-$PK_1$ were greater than those of DR at the same concentration tested (p<0.05). This superior antiradical activity of DRRP might be due to the products produced during the pressurized roasting in addition to the antioxidative compounds originally present in the radish. 5-hydroxyl methyl furfural (5-HMF) known as an intermediate product of the maillard reaction was detected in DRRP (0.57 mg/g), but not from DR. In conclusion, daily consumption of DRRP may prevent oxidative damage by retarding oxidative stress.

Physicochemical Properties and Antioxidant Activities of Maillard Reaction Products from Defatted Hydrolyzed Soybean Protein with Various Sugars (탈지대두단백 산 가수 분해물과 당의 반응에 의하여 생산된 Maillard Reaction Products의 이화학적 특성 및 항산화성)

  • Kim, Yoon-Sook;Moon, Ji-Hye;Kim, Myung-Hee;Choi, Hee-Don;Park, Yong-Kon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.1
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    • pp.62-69
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    • 2009
  • Maillard reaction products (MRPs) were produced from aqueous solution of various sugars with defatted hydrolyzed soybean protein (DFHSP) with different temperatures and pressures. Physicochemical properties of MRPs were investigated; also, DPPH and hydroxyl radical scavenging activity and sensory properties were evaluated. MRPs from ribose and DFHSP had the highest reactivity with larger pH reduce, higher browning index increase and higher antioxidant activity than other MRPs from other sugars. The antioxidant activities were increased with increasing temperatures and pressures of reaction. The highest antioxidant activity and sensory preference were obtained from MRPs with ribose at $140^{\circ}C$ with 2.8 kg/$cm^2$ for 30 mins.