• KSBB Journal
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• 제24권1호
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• pp.41-46
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• 2009

식물성 오일은 다양한 생물제품 생산에 적합한 저가의 원료이다. 식물성 오일의 주성분인 oleic acid와 linoleic acid와 같은 불포화 지방산의 함량은 콩기름의 경우 각각 22%와 55%, 옥수수기름의 경우 26%와 60%, 유채기름의 경우 61%와 21%에 달한다. Hydroxy나 keto group으로 치환된 불포화 지방산들은 다른 지방산에 비해 높은 점도와 반응성 등의 특별한 화학적 특성 때문에 가소제, 계면활성제, 윤활유, 세제 등에 사용되는 유용한 산업 화합물이다. 본 연구에서는 Flavobacterium sp. strain DS5 (NRRL B-14859)를 oleic acid로부터 중간생성물로서 10-hydroxystearic acid (10-HSA)를 거쳐 10-ketostearic acid (10-KSA)로의 전환에 이용하였다. 두 개의 생물전환 생성물인 10-KSA와 10-HSA는 GC, GC-MS, $^1H-NMR$, $^{13}C-NMR$을 이용하여 정량 및 정성분석을 수행하였다. 플라스크 배양에서 10-KSA와 10-HSA의 최대 생산량은 각각 3.4 g/L와 0.5 g/L 이었다. 10-KSA 생산을 위한 최적 glucose 농도 yeast extract 농도, oleic acid 첨가시간, 첨가부피는 각각 20 g/L 이하, 5 g/L 이상, 접종 후 18 h, 0.3 ml/50 ml 이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권3호
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• pp.312-317
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• 2010

Complete oxidation of fatty acid in the liver of ketotic cows was investigated. Serum non-esterified fatty acid (NEFA), beta-hydroxybutyric acid (BHBA) and glucose concentrations were measured using biochemical techniques. Carnitine palmitoyl transferase II (CPT II), 3-hydroxy acyl-CoA dehydrogenase (HAD) and oxaloacetic acid (OAA) concentrations in the liver were detected by ELISA. Serum glucose was lower in ketotic cows than controls (p<0.05). Serum BHBA and NEFA concentrations were higher in ketotic cows than controls (p<0.05). OAA, CPT II, and HAD contents in the liver of ketotic cows were lower than in controls (p<0.05). There were negative correlations between serum NEFA concentration and OAA, CPT II and HAD, but no correlation between serum BHBA concentration and capacity for complete oxidation of fatty acid. Overall, the capacity for complete fatty acid oxidation in the liver of ketotic cows might have been decreased. High serum NEFA concentrations may be unfavorable factors for the pathway of complete oxidation of fatty acid in the liver.

• 대한본초학회지
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• 제35권5호
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• pp.23-31
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• 2020

Objectives : This study was conducted to evaluate the anti-hyperlipidemia effect of Scutellariae Radix, Aucklandiae Radix and Bupleuri Radix(SAB). Methods : FL83B cells were mouse liver hepatocytes, and we used this cell line. FL83B cells were treated with 0.5 mM oleic acid(OA) for 24 h, SAB extract was treated. After OA treatment, intracellular triglyceride (TG) and free fatty acid contents were measured with AdiopoRed™ assay and Free Fatty Acid Quantitation assay kit, respectively. Further, we evaluated several lipogenesis and metabolic markers such as sterol regulatory element-binding transcription factor-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), 3-hydroxy3-methyl-glutaryl CoA reductase (HMGCR), hormone-sensitive lipase (HSL), carnitine palmitoyltransferase (CPT-1), peroxisome proliferator activated receptor alpha (PPARα), and cluster of differentiation (CD36) using RT-PCR and Western-blot analysis. Results : OA markedly increased intracellular TG and free fatty acid, which plays a key role in reducing hepatic lipid accumulation, in FL83B cells. These increases were alleviated by SAB extract. The mRNA and protein expression of Fatty acid(FA) oxidation factors (CPT-1, PPARα), lipolysis factor(HSL), FA transporter(CD36), cholesterol synthesis factors (HMGCoA) and Lipodenesis (SREBP-1c, FAS, and ACC-1) were significantly increased by treatment of SAB extract in the OA-induced fatty liver cell model. Conclusions : In summary, the treat of SAB extract showed a significant reduction of the influx of fatty acids into hepatocytes, promoted the oxidation of fatty acids, and regulated fat synthesis-related factors, thereby regulating the accumulation of TG and free fatty acids.

• 한국응용과학기술학회지
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• 제11권1호
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• pp.53-60
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• 1994

The Synthesis of azobenzene containing long chain fatty acid and poly vinyl alcohol by esterification reaction($C_{n}-Azo-PVA$) was optimized, starting from P-(P'-hydroxy phenyl azo)-benzoic acid and the product of reaction containing azobenzene chromophores was investigated by ultraviolet spectrophotometery in toluene solvent at room temperature. In addition, UV absorption spectra of Langmmuir Blodggett (LB) film deposited on quartz plate have been measured and the structure of these compounds were ascertained by means of Ultraviolet and FT-IR. Recrystallization of reaction product in the solvent results the experimental yield obtained about 22.27% P-(P'-octadecyloxy phenyl azo)-benzoic acid-poly vinyl alcohol. Long chain azobenzene derivative-poly vinyl alcohols are induced phtoisomerization by u, v, and visible light irradiation. The LB film of azobenzene containing long chain fatty acids($C_{18}-Azo-PVA$) are possible of being applied to functional molecular devices such as photomemory and light switching.

• KSBB Journal
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• 제17권3호
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• pp.276-282
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• 2002

Steinernematidae와 Heterorhabditidae속에 속하는 7종의 곤충병원성 선충으로부터 매우 강한 살충성을 갖는 공생박테리아를 분리하여 그 종별 특성을 비교하였다. 분리된 공생박테리아의 종별 배양특성, 살충특성, 단백질 분해 효소의 활성 및 지방산 함량 등이 조사되었다. XR-PC 및 XR-MK의 성장 및 살충성이 가장 우수한 것으로 나타났으며 대수증식기 초기에 살충성이 가장 높은 반면에, 시간이 지남에 따라 살충성도 점차 감소되었다. 이 살충성은 protease 역가와 직접 관련이 없었으나 XR-DR의 경우 다른 종에 비해 배양 3일째 약 4.5배의 최대 활성을 보였다. 그러나 HE-HY의 경우 균체의 성장에 비례하여 protease 역가도 계속 증가하였다. 지방간 함량의 경우 특히 공생박테리아의 종별로 12:0, 14:0, 16:1 cia 5, 17:0 cyclo에서 지방산 함량의 차이를 크게 나타내었으며 hydroxy와 branch 지방산이 전체 지방간의 약 2-15%가지 변화하는 것으로 나타났다.

• 한국식품위생안전성학회지
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• 제13권2호
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• pp.149-154
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• 1998

Vibrio cholerae non-O1 49균주와 V. Vibrio cholerae non-O1, V. mimicus, V. vulnificus와 V. parahaemolyticus의 균체 지방산(fatty acid methyl ester; FAME)을 gas liquid chromatogrphy로 분석하였다. 이들 분석자료를 통계학적으로 처리하여 Vibrio 종과 V. cholerae의 혈청형별 유연성을 비교 검토하였다. 검출된 지방산은 모두 41종이었고 분포량이 많은 것은 16:0, 16:1 cis 9, 18:1 trans 9/16/cis 11과 15:0 iso 2 OH/16:1 cis 9였다. 검출된 지방산 중에서 35종은 V. cholerae를 동정하는데 주요한 인자로 작용되었다 지방산분포를 UPGMA(비가중수리분석)으로 dendrogram을 작성한 결과 V. cholerae non-O!은 V. cholerae O1보다 V. choleare non-O1 중에서 O2, O5, O8, O10, O14, O27, O37, O39, O45와 O69의 총 10종류 혈청을 대상으로 지방산 조성에 의한 유사성을 검토한 결과 유사도가 92% 이상 수준에서 7개의 아종을 형성하여 혈청형과 지방산 조성간에는 유의할 만한 상관관계가 있었다. 따라서 V. cholerae non-O1의 동정 및 역학적인 조사시 지방산 분석은 유용하게 활용될수 있음을 알 수 있다.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1217-1226
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• 2004

A psychrotrophic bacterial strain, Pseudomonas fluorescens BM07, synthesized unsaturated fatty acids (UFA) from fructose in response to lowering of growth temperature, and incorporated them into both polyhydroxyalkanoic acid (PHA) and membrane lipid. The blocking of PHA synthesis by adding 5 mM 2-bromooctanoic acid to the growth medium, containing 70 mM fructose, was found to be a useful means to profile the composition of membrane lipid by gas chromatography. As the growth temperature changed from 35 to $50^{\circ}C$, the total content of two UFA, 3-hydroxy-cis-5­dodecenoic acid ($C_{12:1}$) and 3-hydroxy-cis-7-tetradecenoic acid ($C_{14:1}$), in PHA increased from 31 to 44 $mol\%$. The growth at lower temperatures also led to an increase in the level of two major UFA, palmitoleic acid (C16:1 cis9) and cis-vaccenic acid (C18:1 cis11), in membrane lipid. A fraction of these membrane-lipid UFA was converted to their corresponding cyclopropane fatty acids (CFA). The CFA conversion was a function of culture time, exhibiting biphasic increase before and after entering the stationary phase. However, pH changes in growth media had no effect on the CFA conversion, which is contrary to the case of E. coli reported. The cells grown at $30^{\circ}C$ responded to a cold shock (lowering the medium temperature down to $10^{\circ}C$) by increasing the level of C16:1 cis9 and C 18: I cis II up to that of $10^{\circ}C$-grown control cells and concomitantly decreasing the relative level of cis-9,10­methylenehexadecanoic acid (the CFA converted from C16:1 cis9) from 14 to 8 $mol\%$, whereas the 10-grown cells exhibited little change in the lipid composition when exposed to a warmer environment of $30^{\circ}C$ for 12 h. Based on this one- way response, we suggest that this psychrotrophic strain responds more efficiently and sensitively to a cold shock than to a hot shock. It is also suggested that BM07 strain is a good producer of two unsaturated 3-hydroxyacids, $C_{12:1}\;and\;C_{141:1}$.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.307-308
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• 2005

Bioconverted hydroxy fatty acid from ${\gamma}-linolenic$ acid showed antibacterial activity against Gram-positive bacteria such as Bacillus subtilis (ATCC 6633), Listeria monocytogenes (ATCC 19166), Staphylococcus aureus (ATCC 6538) and S. aureus (KCTC 1916) and one Gram-negative bacteria, Pseudomonas aeruginosa (KCTC 2004) with MIC ranging from 250 to $750\;{\mu}g/ml$ against five of eleven bacteria tested.

• 한국식품과학회지
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• 제49권1호
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• pp.28-34
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• 2017

본 연구를 통하여 P. aeruginosa PR3를 이용하여 DOD를 생산하기 위해 저가의 기질로서 필리핀 너트유가 효과적으로 사용될 수 있음을 확인하였으며 배지에 첨가되는 여러 영양인자들의 영향을 조사하여 DOD 생산성을 크게 향상시킬 가능성이 있음도 확인하였다. 따라서 DOD 생산에 이용되는 올레산을 식물성오일로부터 별도의 생산과정을 거쳐 생산하지 않고 식물성오일자체를 직접 기질로 사용함으로서 PR3 균주를 이용하여 고부가가치의 DOD를 효율적으로 생산할 수 있다는 것을 확인하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.497-499
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• 2005

Bioconverted hydroxy fatty acid, docosahexaenoic (bDHA) obtained from the microbial conversion by Pseudomonas aeruginosa PR3 was evaluated for its in vivo anti-fungal activity. bDHA showed great potential of anti-fungal activity against phytopathogenic fungi tested in this study. bDHA at the concentration of 500 ${\mu}g/ml.$ showed remarkable anti-fungal activity against all the fungus tested.