• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.4
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• pp.491-496
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• 2012

The purpose of this study is to investigate the modulatory effect of wogonin on hydrogen peroxide production in human blastoma SH-SY5Y cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Wogonin significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 10, 25, and 50 ${\mu}M$ in SH-SY5Y (P < 0.05) in dose dependent manner. These results suggest that wogonin has neuroprotective property related with its inhibition of hydrogen peroxide production in PIC-induced neuronal cells.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.09a
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• pp.191-194
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• 2004

The concentration of hydrogen peroxide in soil and effluent was measured to understand the relationship between the profile of hydrogen peroxide and the removal efficiency of phenanthrene in an Electrokinetic-Fenton process. Electrokinetic phenomena were observed in two different conditions for 1, 2, 4, and 7 days; 3.5% and 10% hydrogen peroxide. The concentration of hydrogen peroxide in soil was high near the anode and decreased towards the cathode due to the direction of electroosmosis. The hydrogen peroxide concentration in effluent increased with time, but the value was lower than the initial. The removal efficiency of phenanthrene at 10% hydrogen peroxide was higher than the case at 3.5%. The removal efficiency after 7 days was high(> 97%) in both cases.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.03a
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• pp.131-136
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• 2004

The ongoing developmental studies on the application of hydrogen peroxide for propulsion are briefly reviewed. A detailed design-study of a laboratory scale facility of a hydrogen peroxide mono-propellant engine of 100-N thrust is presented. For the preparation of concentrated hydrogen peroxide, a distillation facility has been realized. Results of water analogy tests are presented. Initial firings using the concentrated hydrogen peroxide were not successful. Low environmental temperature, low contact area of the catalyst pack, and contamination in the hydrogen peroxide were considered to be the reasons. Addressing the first two points resulted in successful firing of the rocket engine.

• Korean Journal of Acupuncture
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• v.28 no.3
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• pp.33-41
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• 2011

Objectives : The purpose of this study is to investigate effects of Scutellariae Radix water extract on hydrogen peroxide production in LPS-induced RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in LPS-induced RAW 264.7 were measured by dihydrorhodamine 123 assay after 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increased hydrogen peroxide production in LPS-induced RAW 264.7 cells for 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). But SC did not represent any significant effect on hydrogen peroxide production in LPS-induced RAW 264.7 cells. Conclusions : These results suggest that Scutellariae Radix, especially produced from South Korea, has the immune-enhancing property related with its increasement of bacteriocidal hydrogen peroxide production in LPS-induced macrophages.

• Korean Journal of Human Ecology
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• v.14 no.3
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• pp.433-439
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• 2005

In this study, we investigated the morphological and physical changes of hair after bleaching treatments with different concentration of hydrogen peroxide in bleaching agent (3, 6, 9, or 12%) and various treatment time (10, 30, or 60 minutes). Tresses of virgin black hair were bleached using a commercial ammonium persulfate-hydrogen peroxide solution. The tensile strength of virgin hair treated with a bleaching agent depended on the concentration of hydrogen peroxide and the treatment time. As the concentration of hydrogen peroxide in bleaching agent went high and the treatment time increased, the tensile strength of the hair increased but the extension degree decreased. The virgin hairs which were not treated by bleaching agent showed morphologically healthy cuticle, whereas the bleached hairs showed swelled and damaged cuticle patterns. The swelling of the hair and the breaks of the cuticles increased positively by the concentration of hydrogen peroxide and the treatment time. These results suggest that exposure to more concentrated hydrogen peroxide with other bleaching components may lead to more severe hair damage.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.36 no.1
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• pp.61-66
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• 2004

Bleached chemithermomechanical pulp (BCTMP) was produced from CTMP of Betula maximowicziana Regel by two staged ozone-hydrogen peroxide bleaching in order to improve the optical properties of high yield pulp. This pulp was used for the evaluation of optical properties improvement, chemical characteristics of lignin in fiber and the relationship between lignin and optical properties in fiber cell wall. Hydrogen peroxide treatment improved the brightness, but the post color number (PC No.). There was little improvement on optical properties by ozone treatment, but this could be improved more by using two staged ozone-hydrogen peroxide bleaching. The hydrogen peroxide treatment did not make any change on chemical characteristics of lignin in cell wall, but by ozone treatment, it was found that the non-aromatic conjugated structure was existed in the surface of cell wall, but this could be removed by hydrogen peroxide treatment in two staged ozone-hydrogen peroxide treatment. Therefore, the optical properties was significantly improved, due to the removal of non-aromatic conjugated structure.

• The Journal of Internal Korean Medicine
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• v.22 no.4
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• pp.557-565
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• 2001

Objectives : This study was carried out to examine toxic effect of Sintongchukeo-tang on cultured mouse spinal sensory neurons inhibited by neurotoxicity induced by hydrogen peroxide. Methods : MTT assay, NR assay, LDH and neurofilament assay were performed after spinal sensory neurons were preincubater with various concentrations of Sintongchukeo-tang water extract before treatment of cells with hydrogen peroxide. Results : Hydrogen peroxide induced ceil degeneration such as the decrease of cell viability was measured by MTT and NR assay in the cultured mouse spinal sensory neurons. Sintongchukeo-tang water extract was effective in the decrease of LDH activities of neurons produced by hydrogen peroxide. Sintongchukeo-tang water extract was effective in the increase of amount of neurofilaments damaged by hydrogen peroxide. Conclusions : From the above results, it is suggested that hydrogen peroxide induces the inhibition of cell viability in cultured mouse spinal sensory neurons and Sintongchukeo-tang water extract was effective in cultured neurons damaged by hydrogen peroxide.

• The Korea Journal of Herbology
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• v.38 no.6
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• pp.45-52
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• 2023

Objectives : Effects of baicalein (BA) on oxidative stress in RAW 264.7 mouse macrophages stimulated with peptidoglycan (PG) and lipopolysaccharide (LPS) were investigated. Methods : RAW 264.7 co-stimulated with LPS and PG were incubated with BA at concentrations of 25 and 50 µM. Incubation time was 18 h, 20 h, 22 h, 24 h, and 26 h. After incubation, the production of hydrogen peroxide in RAW 264.7 was measured with dihydrorhodamine 123 assay. Additionally, RAW 264.7 stimulated with PG were incubated with BA at concentrations of 25 and 50 µM for 24 h. After incubation, NO production was evaluated by griess reagent assay. Results : BA significantly inhibited hydrogen peroxide productions (p <0.05). In details, production of hydrogen peroxide in 'LPS and PG'-stimulated RAW 264.7 treated for 18 h with BA at concentrations of 25 and 50 µM was 91.27% and 89.22% of the control group treated with LPS and PG only, respectively; the production of hydrogen peroxide for 20 h was 92.19% and 90.58%, respectively; production of hydrogen peroxide for 22 h was 91.69% and 89.89%, respectively; production of hydrogen peroxide for 24 h was 92.4% and 90.19%, respectively; production of hydrogen peroxide for 26 h was 91.7% and 89.04%, respectively. Additionally, BA at the concentration of 50 and 100 µM significantly inhibited NO production in PG-induced RAW 264.7 (p <0.05). Conclusions : BA might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in 'LPS and PG'-stimulated RAW 264.7 macrophages.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1495-1499
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• 2008

The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium (WAAF) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with gallic acid, EtOH, LPS, and acetaminophen. WAAF (0${\sim}$400 ug/mL) was treated with gallic acid, EtOH, LPS, acetaminophen. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. WAAF showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, LPS, and acetaminophen in Raw 264.7 Cells. WAAF could be supposed to have the immunological activity related with macrophage's oxidative burst.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.256-260
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• 2004

Melanin was decolorized by lignin peroxidase from Phanerochaete chrysosporium. This decolorization reaction showed a Michaelis-Mentens type relationship between the decolorization rate and concentration of two substrates: melanin and hydrogen peroxide. Kinetic constants of the decolorization reaction were 0.1 OD$\sub$475//min ($V_{max}$) and 99.7 mg/L ($K_{m}$) for melanin and 0.08 OD$\sub$475//min ($V_{max}$) and 504.9 ${\mu}$M ($K_{m}$) for hydrogen peroxide, respectively. Depletion of hydrogen peroxide interrupted the decolorization reaction, indicating the essential requirement of hydrogen peroxide. Pulsewise feeding of hydrogen peroxide continued the decolorizing reaction catalyzed by lignin peroxidase. These results indicate that enzymatic decolorization of melanin has applications in the development of new cosmetic whitening agents.