• Title/Summary/Keyword: hydrocarbon degrading bacteria

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혼합 균주의 유류 분해 특성에 관한 호흡율 연구

  • 목지예;류두현;유병수;유지선;박준석;안병구
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2002.04a
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    • pp.323-326
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    • 2002
  • Oxygen uptake characteristics of soil microcosm added by hydrocarbon degrading bacteria screened from polluted site in Korea was studied. The degradation of TPH was enhanced by additon of nononionic surfactants. The amount of oxygen consumed was decreased at higher concentration. The degradation rate of hydrocarbon was decreased by increasing the hydrcarbon concentration.

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Biodegradation of Phenanthrene by Psychrotrophic Bacteria from Lake Baikal

  • AHN TAE-SEOK;LEE GEON-HYOUNG;SONG HONG-GYU
    • Journal of Microbiology and Biotechnology
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    • v.15 no.5
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    • pp.1135-1139
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    • 2005
  • Psychrotrophic phenanthrene-degrading bacteria were identified in the sediment samples collected from Lake Baikal, Russia. Among 70 phenanthrene-degrading isolates, the seven that had the highest phenanthrene-degradation rates were identified by 16S rDNA sequencing. Isolate P25, identified as the Gram-positive rod-shaped organism Rhodococcus erythropolis, had the highest growth and degradation rate at $15^{\circ}C$. It could remove $26.0\%$ of 100 mg $1^{-1}$ phenanthrene in 20 days at $15^{\circ}C$, and degradation was less at $5^{\circ}C\;and\;25^{\circ}C$. The addition of surfactants to enhance degradation was tested. Brij 30 and Triton X-100 inhibited degradation at all surfactant concentrations tested, but Tween 80 stimulated phenanthrene degradation, especially at low concentrations. When $20{\times}$ CMC (critical micelle concentration) of Tween 80 was added, $38.0\%$ of 100 mg $1^{-1}$ phenanthrene was degraded in 12 days at $15^{\circ}C$. This psychrotrophic phenanthrene-degrading bacterium is a candidate for use in bioremediation of polycyclic hydrocarbon contamination in low temperature environments.

경기만에서 석유분해세균의 분포 및 석유분해능

  • 이정래;황열순;이기승;이건형;김상종
    • Korean Journal of Microbiology
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    • v.30 no.3
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    • pp.187-192
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    • 1992
  • The spatial and temporal distribution of petroleum-degrading bacteria(PDB) was studied at six sampling sites in Kyeonggi Bay of the Yellow Sea fiom March 1990 to October 1991. In addition, petroleum-degrading potcntial of natural ~iiai-ineb acterial population was studied at different culturc contlitions. During the period o f stutly. thc heterotrophic bacterial number and PDB number were n1e;rsured in the range of 7 000-108.400 CFU/nil. 0-2.800 MPN1100 mi. respectively. The spatial tlistribution of PDB wa\ highly affected by presence of petroleum hydrocarbon. In laboratory cxperirncnt. petrolcu~n biodegradation wac enhanced hy addition of yeast cxtracl. cell free cxtr:~ct. anti rnixctl culture of PI)B.

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Biopile의 현장적용을 위한 유류오염토양의 생분해율 평가

  • Yoon, Jeong-Gi;Roh, Hoe-Jeong;Kim, Hyeok;Kim, Jong-Ha;Park, Jong-Gyeom;Lee, Min-Hyo;Jeong, Il-Rok;Koh, Seong-Hwan;Choi, Sang-Il
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.363-367
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    • 2003
  • Batch experiments were performed to determine optimum conditions for biopile. The batch experiments results showed that 12.5 to 17.9% of moisture content was effective to biodegradation of petroleum hydrocarbon regardless of soil texture. Total heterotrophic bacteria populations in the inoculum-treated soil were greater than of the control and nutrient-amended soil in the early stage, but the populations in the inoculum and nutrient-amended soil were not different significantly from those in the latter stage regardless of soil texture. The same trend was observed for petroleum hydrocarbon degrading bacteria populations. The results of the biodegradation capacity experiments showed that there was a decline in the TPH concentrations during the experiments and no significant difference on the biodegradation was observed by treatment in silt soil. Changes of n-C17/pristane and n-C18/phytane ratios in all treated soil were significantly more than those of control. This is a strong indication of biodegradation. The TPH removal rate was calculated at 60% in all treated soil.

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Identification and Characterization of Diesel Degrading Bacteria Isolated from Soil Artificially Contaminated with Diesel Oil (인공오염토양에서 분리한 디젤분해세균의 동정 및 특성)

  • Lee, Su-Jin;Song, In-Geun;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.3
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    • pp.148-156
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    • 2006
  • Potential hydrocarbon degrading bacteria were screened from the site artificially polluted with 20,000 ppm of diesel. Among the isolates, two strains, SJD2 and SJD4, showed higher activities to degrade diesel on the Bushnell-Hass broth medium containing 2% of diesel. 16S rDNA sequence analysis revealed that SJD2 and SJD4 were Bacillus fusifomis and B. cereus, respectively. Both strains were found to grow in a wide range of temperature between $20^{\circ}C-55^{\circ}C$, with the best at $30^{\circ}C-37^{\circ}C$. This is the first report, as far as we know, that B. fusifomis is capable of degrading diesel. We hope that a new isolate, B. fusifomis, will efficiently conduct bioremediation at the contaminated sites with petroleum hydrocarbons.

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Distribution and Biodegradation of Crude oil-Degrading Bacteria in P'ohang Coastal Area (포항근해 원유분해세균의 분포 및 원유분해능)

  • 이창호;권기석;서현호;김희식;오희목;윤병대
    • Journal of Korea Soil Environment Society
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    • v.1 no.2
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    • pp.35-42
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    • 1996
  • Seawater samples were collected from P'ohang coastal area during April 1995 - January 1996. The distribution of total heterotrophic bacteria and crude oil-degrading bacteria (CDB) were studied. In addition, biodegradation of crude oil was investigated through mono and mixed culture. The heterotrophic bacterial distribution was in the range of 4.1 $\times$ $10^4$- 1.2 $\times$ $10^5$ CFU/$m\ell$, respectively. The percent of crude oil-degrading bacteria against total heterotrophic bacteria was 0.05-0.54% which was lower than other marine samples reported. Therefore it could be suggested that the distribution of crude oil-degrading bacteria in the seawater of P'ohang coastal area was highly affected by presence of petroleum hydrocarbon. Taxonomical characteristics of 26 isolates were investigated. The results of identification were showed 7 genera which were Acinetobacter spp., Bacillus spp., Citrobacter spp., Micrococcus spp., Moraxella spp., Rhodococcus spp., and Serratia spp. Appearance of Enterobacteriaceae indicated that the seawater was polluted with wastewater. Also genus of Bacillus had predominant in CDB on P'ohang coastal area. In flask culture, biodegradation of crude oil was enhanced by addition of mixed culture of CDB.

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Characterization of Aromatic Hydrocarbon Degrading Bacteria Isolated from Pine Litter (솔잎 퇴적물에서 추출한 방향족 탄화수소물질 분해 박테리아의 동정)

  • Song, Yoon-Jae
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.333-339
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    • 2009
  • Using a novel pine needle agar, fifteen bacterial species were isolated from pine litter. These bacteria were able to degrade aromatic hydrocarbons derived from lignin and utilize the ortho-cleavage of the $\beta$-ketoadipate pathway to degrade protocatechuate or catechol. A different utilization array of aromatic hydrocarbons by these bacteria was also determined. This study provides the information on bacterial species living in pine litter and suggests that these bacteria have metabolic abilities to utilize aromatic hydrocarbons derived from lignin biodegradation.

Characterization of Diesel Oil-Degrading Bacteria (디젤유 분해균주의 특성 및 토양배양)

  • 안민정;한윤전;임현섭;최기현;권오범;정병철
    • Korean Journal of Microbiology
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    • v.39 no.2
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    • pp.108-113
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    • 2003
  • Diesel oil-degrading bacterial strains were isolated from diesel oil contaminated soil and called HS series (HS1, HS2 and HS3). These strains were identified as Acinetobacter sp. (HS1) and Pseudomonas sp. (HS2 and HS3) based on Biolog test, cellular fatty acid composition, and 16S rDNA sequence analysis. These strains were coltivated in liquid minimal media containing 2% diesel oil, and diesel oil-degrading activity was measured. As result, all strains degraded over 70% of total diesel oil. But PAH (polycyclic aromatic hydrocarbon)- and pris- tane-degrading rate of these strain was below 20% of total PAH and pristane. The HS 1 strain showed highest hydrophobicity and low emulsifying activity among the experimental strains and high diesel oil-degrading activity. From the above-mentioned result, microcosm experiment was performed with the HS1 strain. The HS1 strain showed a degrading activity of over 80% of total diesel oil in microcosm test. And microbial activity was correlated to diesel oil-degrading activity. Therefore, it is suggested that the HS1 strains could be effectively used for the bioremediation for diesel oil.