• Journal of the Korean Society of Safety
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• v.35 no.3
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• pp.105-115
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• 2020

This study compares the difference of fit factors (FF) and visual acuity according to masks and eyeglasses preferences for 54 participants. We the precautions and behaviors of discomfort when wearing masks of eyewear wearers. Contact lens discomfort and priority action of complaints was investigated Glasses fitting factors is Optical Center Height(OH), Vertex Distance(VD) and Pantoscopic Angle(PA). We measured those factors and expressed by the ratio of standard point and change point. Quantitative fit factor was measured by Portacount Pro+ 8038. Also, we selected to 6 exercises among 8 exercises OSHA QNFT (Quantitative Fit testing) protocol to measure the fit factors. The pass/ fail criterion of FF was set at 100. Visual acuity(VA) test chart is developed by Chunsuk Han was used, Descriptive statistics was performed. Descriptive statistics(SAS ver 9.2), it is used geometric means, Wilcoxon analysis(P=0.05) When wearing the mask preferentially, fit factor(FF) was high according to the step of glasses fitting parameter. on the other hand, when the glasses first choice, the visual acuity(VA) was high. there was no significant difference. In the case of fit factor (FF), mask first choice/ glasses first choice is OH (p=0.671/ p=0.332), VD (p=0.602/ p=0.571) and PA (p=0.549/ p=0.607). Visual acuity (VA), mask first choice/ glasses first choice is OH (p=0.753/ p=0.386), VD (p=0.815/ p=0.557) and PA (p=0.856/ p=0.562). The workers of workplace and office chose glasses but occupational health workers and students chose mask. In case of discomforts, it was suggested to remove the mask and tolerate discomforts. The main discomforts and usual action of lens were dryness, hyperemia, foreign body sensation, ophthalmodynia, decreased vision and glasses wearing. Therefore, it is necessary to develop a mask wearing method education program considering glasses fitting and develop a hybrid model that minimizes inconvenience when wearing glasses and a mask at the same time.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.121-121
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• 2003

The standard protocol for the production of transgenic mouse from ES-injected embryo has to process via chimera producing and several times breeding steps, In contrast, tetraploid-ES cell complementation method allows the immediate generation of targeted murine mutants from genetically modified ES cell clones. The advantage of this advanced technique is a simple and efficient without chimeric intermediates. Recently, this method has been significantly improved through the discovery that ES cells derived from hybrid strains support the development of viable ES mice more efficiently than inbred ES cells do. Therefore, the objective of this study was to generate transgenic mice overexpressing human resistin gene by using tetrapioid-ES cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR and cloned into pCR 2.1 TOPO T-vector and constructed in pCMV-Tag4C vector. Human resistin mammalian expression plasmid was transfected into D3-GL ES cells by lipofectamine 2000, and then after 8~10 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 $\mu$sec. (fusion rate : 93.5%) and cultured upto the blastocyst stage (development rate : 94.6%). The 15~20 previously G418-selected ES cells were injected into tetraploid blastocysts, and then transferred into the uterus of E2.5d pseudopregnant recipient mice. To investigate the gestation progress, two El9.5d fetus were recovered by Casarean section and one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, this finding demonstrates that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mouse for the rapid analysis of gene function in vivo.

• Journal of Biomedical Engineering Research
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• v.29 no.2
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• pp.139-145
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• 2008

Many recent studies suggest that the posterior dynamic stabilization(PDS) can be a more physiologically-relevant alternative to the rigid fixation for the patients suffering from low back pain. However, its biomechanical effects or clinically proven efficacies still remain unknown. In this study, we evaluated kinematic behaviors of the lower lumbar spine with the PDS system and then compared to those of the rigid fixation system using finite element (FE) analysis. A validated FE model of intact lumbar spine(L2-L5) was developed. The implanted model was then constructed after modification from the intact to simulate two kinds of pedicle screw systems (PDS and the rigid fixation). Hybrid protocol was used to flex, extend, laterally bend and axially rotate the FE model. Results showed that the PDS systems are more flexible than rigid fixation systems, yet not flexible enough to preserve motion. PDS system allowed $16.2{\sim}42.2%$ more intersegmental rotation than the rigid fixation at the implanted level. One the other hand, at the adjacent level it allowed more range of motion ($2.0%{\sim}8.3%$) than the rigid fixation. The center of rotation of the PDS model remained closer to that of the intact spine. These results suggest that the PDS system could be able to prevent excessive motion at the adjacent levels and restore the spinal kinematics.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37B no.11
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• pp.1004-1013
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• 2012

The legacy voice broadcast systems are used to broadcast the voice over an entire space or a specific zone. these broadcast systems generate unnecessary noise and waste of resources. In this paper, we propose a ubiquitous voice message broadcast system called uPaging, by combining the technique of location-awareness and the voice message delivery service in ubiquitous sensor network environment. In uPaging system, the wire/wireless hybrid network is used to implement the network system. Also, in order to actualize the location-awareness service, we use the Bidirectional Location ID-Exchange protocol was suggested by our previous research. the uPaging system can deliver the voice to a selected user or the location in which the user is present by this location awareness.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.9A
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• pp.837-843
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• 2006

IEEE802.15.3 and IEEE802.15.4 have defined the hybrid MAC protocols based on TDMA and CSMA where a multi-frame TDMA structure is employed so that multiple data frames can be transmitted within one timeslot to guarantee minimum delay bounds of isochroous traffic. However, TDMA has an intrinsic problem that cannot dynamically allocate optimal length of timeslot to each station. Therefore the idle timeslot can be produced by stations when each transmission queue is instantaneously empty during its timeslot, which would waste lots of timeslots especially in the multi-frame TDMA systems. In this paper, we propose a more flexible multiple-access scheme for the multi-frame TDMA system based on the concept of virtual slot which is accessible by every station with the highest priority for slot owner and lower priority for other stations. Finally, our simulation results from various environments show that proposed scheme can achieve magnitude improvement of total system throughput and average message delay by maximizing channel utilization.

• Journal of Communications and Networks
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• v.6 no.3
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• pp.269-279
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• 2004

A new online multi-layer integrated routing (MLIR) scheme that combines IP (electrical) layer routing with WDM (optical) layer routing is investigated. It is a highly efficient and cost-effective routing scheme viable for the next generation integrated optical Internet. A new simplified weighted graph model for the integrated optical Internet consisted of optical routers with multi-granularity optical-electrical hybrid switching capability is firstly proposed. Then, based on the proposed graph model, we develop an online integrated routing scheme called differentiated weighted fair algorithm (DWFA) employing adaptive admission control (routing) strategies with the motivation of service/bandwidth differentiation, which can jointly solve multi-layer routing problem by simply applying the minimal weighted path computation algorithm. The major objective of DWFA is fourfold: 1) Quality of service (QoS) routing for traffic requests with various priorities; 2) blocking fairness for traffic requests with various bandwidth granularities; 3) adaptive routing according to the policy parameters from service provider; 4) lower computational complexity. Simulation results show that DWFA performs better than traditional overlay routing schemes such as optical-first-routing (OFR) and electrical-first-routing (EFR), in terms of traffic blocking ratio, traffic blocking fairness, average traffic logical hop counts, and global network resource utilization. It has been proved that the DWFA is a simple, comprehensive, and practical scheme of integrated routing in optical Internet for service providers.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.5
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• pp.1303-1315
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• 2012

In the draft of the IEEE 802.11s standard, a tree topology is established by the proactive tree-building mode of the Hybrid Wireless Mesh Protocol (HWMP). It is used for cases in which the root station (e.g., gateway) is an end point of the majority of the data connections. In the tree topology, the root or central stations (e.g., parent stations) are connected to the other stations (e.g., leaves) that are one level lower than the central station. Such mesh stations are likely to suffer heavily from contention in bottleneck links when the network has a high traffic load. Moreover, the dependence of the network on such stations is a point of vulnerability. A failure of the central station (e.g., a crash or simply going into sleep mode to save energy) can cripple the whole network in the tree topology. This causes performance degradation for end-to-end transmissions. In a connected mesh topology where the stations having two or more radio links between them are connected in such a way that if a failure subsists in any of the links, the other link could provide the redundancy to the network. We propose a scheme to utilize this characteristic by organizing the network into concentric tiers around the root mesh station. The tier structure facilitates path recovery and congestion control. The resulting mode is referred to as Tier-based Proactive Path Selection Mode (TPPSM). The performance of TPPSM is compared with the proactive tree mode of HWMP. Simulation results show that TPPSM has better performance.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.11B
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• pp.1607-1617
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• 2010

In this paper we propose MA-CBRP, mesh adaptive algorithm for wireless mesh networks. MA-CBRP is a hybrid algorithm based on ad-hoc CBRP protocol. In MA-CBRP, the mesh router periodically sends the ANN message as like Hello-message in CBRP. ANN message allows to all clients periodically store a route towards the mesh-router and renewal information in their routing cache. While CBRP periodically reply Hello-message, MA-CBRP does not reply to achieve less overhead. After receiving ANN message, mesh client send JOIN message to mesh router when the route towards mesh router changed. at the same time Register the entry to mesh router, it can achieve to reduce overhead of control the route and shorten the time to find route. consequently, MA-CBRP shows 7% reduced overhead and shortened time to find route than CBRP with regardless of clients number.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.3
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• pp.335-343
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• 2018

Objective: Remarkable difference in cellular activity was found between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes in cell manipulation protocol. This study subsequently examined whether post-thaw subculture of early subpassaged ESC lines could further affect the activity of the ESCs. Methods: Fresh (as a control treatment) or cryopreserved F1 hybrid (B6CBAF1) early ESC lines (C57BL/6xCBA) of the 4 (P4) or the 19 passage (P19) were subcultured once, twice or six times under the same condition. The post-thaw survival of the ESCs was monitored after the post-treatment subculture and the ability of cell proliferation, reactive oxygen species (ROS) generation, apoptosis and mitochondrial ATP synthesis was subsequently examined. Results: Regardless of the subculture number, P19 ESCs showed better (p<0.05) doubling time and less ATP production than P4 ESCs and such difference was not influenced by fresh or cryopreservation. The difference between P4 and P19 ESC lines became decreased as the post-treatment subculture was increased and the six times subculture eliminated such difference. Similarly, transient but prominent difference in ROS production and apoptotic cell number was detected between P4 and P19 ESCs only at the 1st subculture after treatment, but no statistical differences between two ESC lines was detected in other observations. Conclusion: The results of this study suggest that post-thaw subculture of ESCs under the same environment is recommended for standardizing their cellular activity. The activity of cell proliferation ability and ATP synthesis can be used as parameters for quality control of ESCs.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.12
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• pp.2811-2817
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• 2010

In this paper we propose the QAC-MAC that supports Quality of Service(QoS) and saves energy resources of the sensor node, and hence prolonging the lifetime of the sensor network with multiple sink nodes. Generally, the nodes nearest to the sink node often experience heavy congestion since all data is forwarded toward the sink through those nodes. So this critically effects on the delay-constraint data traffics. QAC-MAC uses a hybrid mechanism that adapts scheduled scheme for medium access and scheduling and unscheduled scheme based on TDMA for no data collision transmission. Generally speaking, characteristics of the real-time traffic with higher priority tends to be bursty and has same destination. QAC-MAC adapts cross-layer concept to rearrange the data transmission order in each sensor node's queue, saves energy consumption by allowing few nodes in data transmission, and prolongs the network lifetime.