• Title/Summary/Keyword: hybrid media

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Intergeneric Transfer of Isolated Nuclei from Lentinus edodes into Protoplasts of Pleurotus florida (사철느타리버섯 원형질체내에 표고 핵의 전이)

  • Yoo, Young-Bok;Shin, Pyung-Gyun
    • The Korean Journal of Mycology
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    • v.24 no.2 s.77
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    • pp.111-126
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    • 1996
  • Transfer of the isolated nuclei from Lentinus edodes into protoplasts of Pleurotus florida was induced with polyethlene glycol (PEG) and $CaCl_2$. The intergeneric transfer products were classified into nuclear hybrid, heterokaryon or synkaryon, and reconstituted cell. These progenies except nuclear hybrids formed mature fruiting bodies on sawdust rice bran medium. Formation of fruit bodies was influenced by several factors such as light, temperature, nutrition and physic state of the culture media. Most of fruiting body characters were similar to those of P. florida in synkaryon and L. edodes in reconstituted cell, respectively. All these basidiocarps had clamp connections though initial heterokaryon colonies were lacking. Isozyme patterns of intergeneric progenies were quite different from those of parents. DNA polymorphisms of transfer products were also compared by random amplified polymorphic DNAs (RAPD) analysis based on polymerase chain reaction. The RAPD patterns were different from those of donor and recipient. DNA fingerprints ranged in size from 0.25 to 4.0 Kb. On the basis of RAPD, the transfer products were classified into five groups. Two synkaryon were analysed with distribution of progenies and segregation of genetic markers by random spore analyses. The genetic markers were segregated into wild type and riboflavine requiring auxotrophs.

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A Comparison Study of RNN, CNN, and GAN Models in Sequential Recommendation (순차적 추천에서의 RNN, CNN 및 GAN 모델 비교 연구)

  • Yoon, Ji Hyung;Chung, Jaewon;Jang, Beakcheol
    • Journal of Internet Computing and Services
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    • v.23 no.4
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    • pp.21-33
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    • 2022
  • Recently, the recommender system has been widely used in various fields such as movies, music, online shopping, and social media, and in the meantime, the recommender model has been developed from correlation analysis through the Apriori model, which can be said to be the first-generation model in the recommender system field. In 2005, many models have been proposed, including deep learning-based models, which are receiving a lot of attention within the recommender model. The recommender model can be classified into a collaborative filtering method, a content-based method, and a hybrid method that uses these two methods integrally. However, these basic methods are gradually losing their status as methodologies in the field as they fail to adapt to internal and external changing factors such as the rapidly changing user-item interaction and the development of big data. On the other hand, the importance of deep learning methodologies in recommender systems is increasing because of its advantages such as nonlinear transformation, representation learning, sequence modeling, and flexibility. In this paper, among deep learning methodologies, RNN, CNN, and GAN-based models suitable for sequential modeling that can accurately and flexibly analyze user-item interactions are classified, compared, and analyzed.

A Study on the Display Considerations of RDA Resource Type (RDA 자원유형 디스플레이를 위한 고려사항에 관한 연구)

  • Lee, Mihwa
    • Journal of the Korean Society for information Management
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    • v.33 no.1
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    • pp.33-52
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    • 2016
  • This study was to find the display considerations of RDA resource type - content type, media type, and carrier type. The Literature review, the case study, and the survey were used as the research method. 5 display strategies were suggested in this study. First, content and carrier types were better displayed than all 3 types of RDA resources. Second, two kinds of algorithm should be considered for RDA resource icon display. One was the combination of the terms of carrier types plus content types icon. The other was the combination of carrier types icon and content types icon in which the terms reflecting types must be included. Third, the subfield of 33x must be used for the paired display of content type and carrier type of multi-types resources. Fourth, in brief display, resource type icon was better positioned on the left and upper side and in detailed display, resource types were better located in description area. Fifth, 'format' was used as display indication phrase. This study would contribute to the design for the resource display by suggesting the practical display considerations of RDA resource type.

$M^2$ MAC: MAC protocol for Real Time Robot Control System based on Underwater Acoustic Communication ($M^2$ MAC(Message Merging): 수중음파통신 기반의 실시간 로봇 제어 시스템을 위한 MAC 프로토콜)

  • Kim, Yung-Pyo;Park, Soo-Hyun
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.48 no.6
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    • pp.88-96
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    • 2011
  • Underwater acoustic communication is applicable in various areas, such as ocean data collection, undersea exploration and development, tactical surveillance, etc. Thus, robot control system construction used for underwater-robot like AUV or ROV is essential in these areas. In this paper, we propose the Message Merging MAC($M^2$-MAC) protocol, which is suitable for real time robot control system, considering energy efficiency in important parts of underwater acoustic sensor network constitution. In this proposed MAC protocol, gateway node receives the data from robot nodes according to the time slots that were allotted previously. And messages delivered from base-station are generated to one MAC frame by buffering process. Finally, generated MAC frames are broadcasted to all robot nodes in the cluster. Our suggested MAC protocol can also be hybrid MAC protocol, which is successful blend of contention based and contention-free based protocol through relevant procedure with Maintenance&Sleep (M&S) period, when new nodes join and leave as an orphan. We propose mathematical analysis model concerned about End-to-End delay and energy consumption, which is important factor in constructing real-time robot control system. We also verify the excellence of performance according to comparison of existing MAC protocols with our scheme.

Selection of Agaricus bitorquis hybrid strains based on RAPD analysis (RAPD분석을 통한 여름양송이(Agaricus bitorquis)의 교잡주 선발)

  • Oh, Youn-Lee;Jang, Kab-Yeul;Kong, Won-Sik;Shin, Pyung-Gyun;Kim, Eun-Sun;Oh, Min ji;Choi, In-Geol
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.243-249
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    • 2015
  • Agaricus bitorquis is an edible white mushroom of the genus that is cultivated at high temperature($25{\pm}1^{\circ}C$) unlike A. bisporus is cultivate at $16{\pm}2^{\circ}C$. Unlike Agaricus bisporus, an edible white A. bitorquis mushroom is cultivated at high temperature ($25{\pm}1^{\circ}C$). Most farmers cultivate this mushroom for a long cultivation period in Korea. For this reason, we made heterokayons to develop a new cultivar that generate fruitbodies for short cultivation period. Over one hundred SSIs(single spores isolates) were collected from selected A. bitorquis ASI1151 and ASI1349 strains. Seventy-three SSIs were germinated on CDA(compost dextrose agar) media after 20 days (minimum) or 83 days (maximum) incubation under different media condition. The mycelial growth rate of germinated SSIs was different. 9 homokaryons in ASI 1151 and 11 homokaryons in ASI 1349 from SSIs were selected by OPN-02 primer in RAPD analaysis. Also this primer was used to select heterokaryon that cross among each homokaryon with compatible locus. Therefore 44 compatible matings were confirmed of 99 crossed lines.

Modeling of Elastodynamic Problems in Finite Solid Media (유한 고체내 탄성동역학 문제의 모델링)

  • Cho, Youn-Ho
    • Journal of the Korean Society for Nondestructive Testing
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    • v.20 no.2
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    • pp.138-149
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    • 2000
  • Various modeling techniques for ultrasonic wave propagation and scattering problems in finite solid media are presented. Elastodynamic boundary value problems in inhomogeneous multi-layered plate-like structures are set up for modal analysis of guided wave propagation and numerically solved to obtain dispersion curves which show propagation characteristics of guided waves. As a powerful modeling tool to overcome such numerical difficulties in wave scattering problems as the geometrical complexity and mode conversion, the Boundary Element Method(BEM) is introduced and is combined with the normal mode expansion technique to develop the hybrid BEM, an efficient technique for modeling multi mode conversion of guided wave scattering problems. Time dependent wave forms are obtained through the inverse Fourier transformation of the numerical solutions in the frequency domain. 3D BEM program development is underway to model more practical ultrasonic wave signals. Some encouraging numerical results have recently been obtained in comparison with the analytical solutions for wave propagation in a bar subjected to time harmonic longitudinal excitation. It is expected that the presented modeling techniques for elastic wave propagation and scattering can be applied to establish quantitative nondestructive evaluation techniques in various ways.

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The Reinterpretation of Comic-Animation by Content Users - The Reproductions in Korean Cosplay Culture (콘텐츠 유저에 의한 만화-애니메이션의 재해석에 관한 연구 - 한국 코스프레 문화에서의 재생산)

  • Yoon, Wn-Ho
    • Cartoon and Animation Studies
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    • s.41
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    • pp.487-510
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    • 2015
  • In recently, thanks to the development of computer and internet, and reinforcement of digital media literacy of ordinary people, many users of comics and animations recombine contents into various ways, including UCCs. In a glance, it seems these outputs violates the copyrights of contents. But in reality, they helps the growth of the ecosystem of contents market, with increase of contents users. The reason of these reproductions inclines the characteristics of animation media. Animation, which are constituted by plastic symbols, auditory symbols, and narrative symbols, and Comic, which supports stories of Animations, affects as 'Imaginary signifier'. These signifiers helps users of contents to dismantle and reassemble stories. These aspects are seen in doujin culture, animation soundtrack fandom, and voice actor fandom. But In this article, we will concentrate into cosplay culture. Cosplay culture shows more active appearances than another fandoms. In especially, cosplay stage and daily cafe show more active reproductions of characters and communications. However, they are done by youth fans, which has not enough financial abilities: therefore, events are not sustainable and discontinued. And they have another limits, like lack of opportunity of performance. In the reality of Korean society, which needs of community making by community member, and hybrid culture, comics and animation productions in Korea are in need of access positively and encourage reproductions of active receivers of animations.

AutoML Machine Learning-Based for Detecting Qshing Attacks Malicious URL Classification Technology Research and Service Implementation (큐싱 공격 탐지를 위한 AutoML 머신러닝 기반 악성 URL 분류 기술 연구 및 서비스 구현)

  • Dong-Young Kim;Gi-Seong Hwang
    • Smart Media Journal
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    • v.13 no.6
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    • pp.9-15
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    • 2024
  • In recent trends, there has been an increase in 'Qshing' attacks, a hybrid form of phishing that exploits fake QR (Quick Response) codes impersonating government agencies to steal personal and financial information. Particularly, this attack method is characterized by its stealthiness, as victims can be redirected to phishing pages or led to download malicious software simply by scanning a QR code, making it difficult for them to realize they have been targeted. In this paper, we have developed a classification technique utilizing machine learning algorithms to identify the maliciousness of URLs embedded in QR codes, and we have explored ways to integrate this with existing QR code readers. To this end, we constructed a dataset from 128,587 malicious URLs and 428,102 benign URLs, extracting 35 different features such as protocol and parameters, and used AutoML to identify the optimal algorithm and hyperparameters, achieving an accuracy of approximately 87.37%. Following this, we designed the integration of the trained classification model with existing QR code readers to implement a service capable of countering Qshing attacks. In conclusion, our findings confirm that deriving an optimized algorithm for classifying malicious URLs in QR codes and integrating it with existing QR code readers presents a viable solution to combat Qshing attacks.

Differential Expression of a Chimeric nos-npt II Gene in 9 Years Old Hybrid Poplars (Populus koreana x P. nigra)

  • Noh, Eun Woon;Lee, Jae Soon;Choi, Young Im;Lee, Hyo Shin;Bae, Eun Kyung;Lee, Ji Hee
    • Journal of Plant Biotechnology
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    • v.6 no.1
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    • pp.15-19
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    • 2004
  • The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

Hardware Architecture for PC-based MPEG-4 Video CODEC (PC 기반 MPEG-4 비디오 코덱 구현을 위한 하드웨어 아키텍쳐)

  • 곽진석;임영권;박상규;김진웅
    • Journal of Broadcast Engineering
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    • v.2 no.2
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    • pp.86-93
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    • 1997
  • Fast growth of multimedia applications requires new functions for video data processing. such as obj;cted-based video representation and manipulation. which are not supported by 11PEG-l and 11PEG-2. To support these requirements. 11PEG-4 video coding allows users to manipulate every video object easily by decomposing a scene into several video objects and coding each of them independently. However. the large amount of computations and flexible structure of 11PEG-4 video CODEC make it difficult to be implemented by either the general purpose DSP or a dedicated VLSI. In this paper, we propose a hardware architecture using a hybrid of a high performance programmable DSP and an application specific IC to implement a flexible 11PEG-4 video codec requiring the large amount of computations. The application specific IC has the functions of motion estimation and compensation.

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