Browse > Article
http://dx.doi.org/10.14480/JM.2015.13.3.243

Selection of Agaricus bitorquis hybrid strains based on RAPD analysis  

Oh, Youn-Lee (Department of Biotechnology College of Life Sciences and Biotechnology Korea University)
Jang, Kab-Yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Shin, Pyung-Gyun (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Kim, Eun-Sun (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Oh, Min ji (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Choi, In-Geol (Department of Biotechnology College of Life Sciences and Biotechnology Korea University)
Publication Information
Journal of Mushroom / v.13, no.3, 2015 , pp. 243-249 More about this Journal
Abstract
Agaricus bitorquis is an edible white mushroom of the genus that is cultivated at high temperature($25{\pm}1^{\circ}C$) unlike A. bisporus is cultivate at $16{\pm}2^{\circ}C$. Unlike Agaricus bisporus, an edible white A. bitorquis mushroom is cultivated at high temperature ($25{\pm}1^{\circ}C$). Most farmers cultivate this mushroom for a long cultivation period in Korea. For this reason, we made heterokayons to develop a new cultivar that generate fruitbodies for short cultivation period. Over one hundred SSIs(single spores isolates) were collected from selected A. bitorquis ASI1151 and ASI1349 strains. Seventy-three SSIs were germinated on CDA(compost dextrose agar) media after 20 days (minimum) or 83 days (maximum) incubation under different media condition. The mycelial growth rate of germinated SSIs was different. 9 homokaryons in ASI 1151 and 11 homokaryons in ASI 1349 from SSIs were selected by OPN-02 primer in RAPD analaysis. Also this primer was used to select heterokaryon that cross among each homokaryon with compatible locus. Therefore 44 compatible matings were confirmed of 99 crossed lines.
Keywords
Agaricus bisporus; Brown cultivar; Morphological and cultivational characteristics;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fritsche G. 1978. Cultivated fungi in Agaricales. The biology and cultivation of edible mushroom. p240-243, ed. Chang ST and Hayes WA. Academic Press.
2 Hintz W, Anderson JB, Horgen PA. 1988. Nuclear Migration and Mitochondrial Inheritance in the Mushroom Agaricus bitorquis. Genetics. 119(1): 35-41.
3 Hurtado S & Ramstedt M. 2002. AFLP comparison of distant Melampsora epitea (willow rust) populations. Mycol Res. 106(12): 1400-1407.   DOI   ScienceOn
4 Jzsef G, David MG, Daniel JR. 2004. Molecular evolution of Agaricus species based on ITS and LSU rDNA sequences. Mycological Progress. 3(2): 157-176.   DOI
5 Pongam P, Osborn TC & Williams PH. 1999. Assessment of Genetic Variation Among Leptosphaeria maculans Isolates Using Pathogenicity Data and AFLP Analysis. Plant Disease. 83(2): 149-154.   DOI   ScienceOn
6 RAPER CA. 1976. Sexuality and Life-cycle of the Edible, Wild Agaricus bitorquis. Microbiology. 95(1): 54-66.
7 Special crop production in 2014. p60-117, ed. Ministry of Agriculture, Food and Rural Affair Press.
8 Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, frijters A, Pot J, Peleman J, Kuiper M & Zabeau. 1995. AFLP: a new technique for DNA fingerprinting. M, Nucl Acids Res. 23(21): 4407-4414.   DOI
9 Yadav MC, Sharma RK, Singh SK, Mohapatra T. 2006. Molecular Differentiation of Sexually Incompatible Strains of Agaricus bitorquis Using RAPD and AFLP Markers. J. Plant Biochemistry & Biotechnology. 15: 109-116.   DOI
10 Zeng W, Song SY, Wang ZS & Su WJ. 1999. RAPD analysis of two genetic families of Agaricus bisporus. Mycosystema. 27(2): 132-134.
11 Castle AJ, Horgen PA and Anderson JB. 1987. Restriction fragment length polymorphisms in the mushrooms Agaricus brunnescens and Agaricus bitorquis. Appl Environ Microbiol. 53(4): 816-822.
12 Dickhardt R. 1985. Homokaryotization of Agaricus bitorquis (Quel.) Sacc. and Agaricus bisporus (Lange) Imb. Theor Appl Genet. 70: 52-56.
13 Elliott TJ. 1978. Comparative Sexuality in Agaricus Species. Microbiology. 107: 113-122.