• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.128-128
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• 1993

총 40종 생약의 hyaluronidase에 대한 저해 효과를 검색한 결과, 백지, 오수유, 향부자, 섬백리향, 배풍등, 황금, 황련, 진피, 소리쟁이, 반디나물 등 10종 생약에서 저해 활성을 관찰할 수 있었다. 또한 권백의 n-BuOH 분획에서 2개의 biflavonoid 성분인 amentoflavone, isocryptomerin을 분리하였으며, chloroform 분획에서 cryptomerin B를 분리하였다. 이들 화합물의 화학구조는 각종 기기분석 data를 이용하여 결정하였으며, 이들 화합물중 cryptomerin B는 Selaginellaceae에서 처음으로 분리 보고되는 화합물이다. 현재 이들 화합물의 생리 활성에 대하여 검사중이다.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.200.3-200.3
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• 2003

Reactive oxygen species(ROS) are produced at a high rate continuously as a by-product of aerobic metabolism. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. High level of hyaluronic acid with decreased molecular weight has been detected in patients with inflammatory diseases including rheumatoid arthritis. Hyaluronidase is an endohexosaminidase that initiates the degradation of hyaluronic acid with high molecular weight. Prunus persica Batsch var. davidiana Maximowicz has been known as a korean folk medicine for treatment of neuritis and rheumatism. (omitted)

• Journal of the korean veterinary medical association
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• v.5 no.2
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• pp.16-19
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• 1961

• Fisheries and Aquatic Sciences
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• v.23 no.3
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• pp.6.1-6.9
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• 2020

Background: Halophyte plant (HPs), a salt-resistant flora, has been reported to provide several health benefits, but the knowledge of its cosmeceutical potential is still ambiguous. Here, 70% ethanol extracts of 22 HPs collected from along the coast of South Korea were investigated for their potentials of antioxidant, anti-aging, and whitening properties for use as materials in novel cosmeceuticals. Methods: Antioxidant activities were determined by DPPH (1,1-diphenyl-2-pricrylhydrazyl) free radical and hydrogen peroxide scavenging assays, and skin aging-related enzyme activities (anti-elastase, anti-collagenase, anti-hyaluronidase, and anti-tyrosinase) were evaluated by using the spectrophotometric method. Results: Among the 22 HPs, we found that Ischaemum antephoroides f. coreana and Atriplex gmelinii extracts presented the strongest scavenging effects against DPPH free radical and hydrogen peroxide, respectively. Our finding additionally suggested that Salicornia europaea extract might provide a major source of anti-elastase and anti-hyaluronidase; meanwhile, Rosa rugosa extract showed the highest anti-collagenase effect. Furthermore, the highest tyrosinase inhibitory activity was possessed by Spartina anglica extract. Conclusion: These findings may suggest that halophyte plants showing biological activities may be potent inhibitors of tyrosinase, elastase, collagenase, and hyaluronidase and could be useful for application in cosmeceuticals.

• Korean Journal of Medicinal Crop Science
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• v.12 no.4
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• pp.289-294
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• 2004

Reactive oxygen species (ROS) are continuously produced at a high rate as a by-product of aerobic metabolism. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. High level of hyaluronic acid with decreased molecular weight has been detected in patients with inflammatory diseases including rheumatoid arthritis. In this study, we have conducted to investigate the antioxidant and hyaluronidase inhibitory activities of Prunus persica Batsch var. davidiana Maximowicz in order to screen the bioactive substances which can be developed as possible anti-inflammatory agents. Among the extracts of Prunus persica Batsch var. davidiana Maximowicz, EtOAc extract exhibited the strongest effect on antioxidant experiment such as DPPH, Ferric- Thiocyanate and Rancimat. Also, EtOAc extract showed a potent hyaluronidase inhibitory activity.

• Food Science and Biotechnology
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• v.18 no.1
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• pp.267-270
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• 2009

Naringenin is a bioactive flavanone containing antioxidative, anti-inflammatory, and anticarcinogenic properties. The inhibitory effects on hyaluronidase of naringenin and its novel derivatives were evaluated. Among these flavonoids at $200{\mu}M$ concentration, 7-O-butyl naringenin had the highest inhibitory effect on hyaluronidase with 44.84%. In addition, For naringenin at concentrations of 0, 150, and $190{\mu}M$, the apparent Michaelis constants ($_{app}K_m$) were calculated to be $0.60{\pm}0.02$, $0.43{\pm}0.02$, and $0.41{\pm}0.01\;mg/mL$ of substrate, respectively; for 7-O-butyl naringenin at 0, 20, and $30{\mu}M$ concentrations, those were $0.44{\pm}0.03$ and $0.27{\pm}0.03\;mg/mL$, respectively. The $V_{max}$ values at 150 and $190{\mu}M$ naringenin were $0.59{\pm}0.02$ and $0.56{\pm}0.01\;mg/mL/min$, respectively; and those at 20 and $30{\mu}M$ 7-O-butyl naringenin were $0.50{\pm}0.02$ and $0.33{\pm}0.02\;mg/mL/min$, respectively. However, the slopes of each inhibitory reaction were not significantly different. Therefore, naringenin and 7-O-butyl naringenin were shown to be uncompetitive inhibitors. These results demonstrate the potential use of 7-O-butyl naringenin as an anti-inflammatory substance.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.111-124
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• 2006

Purpose : This study was conducted to investigate the dose dependent effects of Panax Ginseng on the reproductive functions in mice. Methods : We used the 8-week-old mice, and administered 0.2ml extract solution of Panax Ginseng in the different concentration once a day for 60 days. The control group was administered 0.2ml normal saline in the same way and duration. We counted the total, motile and normal sperm number of the cauda epididymis and measured the activities of sperm hyaluronidase, peroxidase and catalase of the isolated testis tissues. And we observed histological changes of surgically isolated testis by histochemical methods. Results : All Panax Ginseng extract solution groups showed significantly dose dependent differences in the total number, the motility and normality of sperms compared with the control group, respectively. In the histological analysis of the testicular tissues, all Panax Ginseng extract solution groups showed the enlargement of testicular lobe diameter and apparent angiogenesis between seminiferous tubules. And the activity of typical sperm enzyme, hyaluronidase, was significantly increased in the Panax Ginseng extract solution groups compared to the control group. In the antioxidant activity analysis, the activities of peroxidase and catalase were significantly increased in the Panax Ginseng extract solution groups compared to the control group. Conclusion : This study shows that Panax Giseng has the beneficial effects on the concentration, morphology and motility of sperm, the activities of sperm hyaluronidase, testicular peroxidase and catalase. We can suggest that Panax Ginseng be useful for the treatment of male infertility.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.63-63
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• 2019

The thinning Green ball apple was extracted using water and ethanol and a phenolic concentration of thinning Green ball apple was $50-200{\mu}g/mL$. The water and ethanol extracts of thinning Green ball apple showed 94.69% and 92.24% 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and 100.30% and 99.16% 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity at phenolic concentration of $200{\mu}g/mL$, respectively. The water and ethanol extracts of thinning Green ball apple showed antioxidant protection factor of 1.76 antioxidant protection factor and 1.76 antioxidant protection factor, respectively. The water and ethanol extracts showed 101.46% and 99.64% anti-oxidative effect on thiobarbituric acid reactive substances at phenolic concentration of $200{\mu}g/mL$. Hence, the water and ethanol extracts of thinning Green ball apple can be considered a potential anti-oxidant. The water and ethanol extracts showed 33.28% and 32.14% hyaluronidase inhibition, respectively, at phenolic concentration of $150{\mu}g/mL$. The water and ethanol extracts showed 47.33% and 40.92% elastase inhibition and 46.19% and 65.58% collagenase inhibition at phenolic concentration of $200{\mu}g/mL$, respectively. About these experiments, thinning Green ball apple was found to exhibit anti-oxidation activity as well as hyaluronidase, elastase and collagenase inhibitory activities. Therefore, thinning Green ball apple can be considered a potential source for functional food.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.3
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• pp.275-285
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• 2022

In this study, we prepared two extracts using 1,3-butylene glycol (DBG) and ethanol (DET), with Diospyros kaki and determined the anti-oxidant, anti-aging, and whitening effects in vitro. Anti-oxidant activity was measured by DPPH and superoxide dismutase (SOD) method, and as a result, both DBG and DET extracts confirmed their anti-oxidant properties by exhibiting significant DPPH scavenging and SOD-like activities. For anti-aging activity, we measured elastase and hyaluronidase inhibition, and the inhibition of MMP-1 expression. Both DBG and DET significantly inhibited elastase and hyaluronidase activities dose-dependently, and MMP-1 expression was also reduced in both extracts. We also measured the whitening effects with tyrosinase activity and melanin production, but only DBG showed a decrease in tyrosinase. In summary, D. kaki extract has strong antioxidant, anti-aging, and whitening functions, and it is believed that it can be used as a cosmetic material in the future.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.232-232
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• 1994

인삼이 면역계 질환의 일종인 과민성 반응에 대한 치료약으로서의 가능성을 연구하기 위해서 in viva와 in vitra실험을 수행하였다. in vitra실험으로서는 hyaluronidase의 활성을 지표로 삼았으며, in viva실험에서는 mouse이각, rat등피부, 복강 비만세포, 그리고 guinea pig의 ileum에서 면역반응에 대한 아답타겐의 작용을 관찰하였다. in uitra 실험에서는 hyaluronidase와 histamine 분비를 항알레르기 작용의 지표로 사용하였다. hyaluronidase의 경우 그 활성은 Cacl2로 활성화 시키기 전이나 후에 모두 아답타겐(100, 50, 및 10 mg/ml)에 의해서 현저하게 억제되었다. 한편 rat 복강 비만 세포에서의 histamine유리 시험에서는 아답타겐(0.5, 1, 5mg/ml)이 histamine유리가 증가되었다. Guinea pig의 ileum 평활근에서는 아답타겐이 평활근 자극 작용없이 항히스타민 효과률 나타내었다. in uiua 실험의 첫 단계로 우리는 혈관 투과성에 미치는 아답타겐의 효과를 mouse 이각과 rat 등피부에서 관찰하였다. Mouse이각에서의 실험에서는 hoistamine, serotonin, 그리고 LTC4로 challenge하여 관찰한 결과 histamine과 serotonin의 경우, 혈관투과성 항진은 아답타겐(50-400mg/m1)에 의해서 유의성있게 억제되었으나, rat 등 피부에서는 mouse에서와는 달리 48-hr PCA가 증가 되었고, histamine에 의한 혈관투과성 항진은 아답타겐에 의해 영향을 받지 않았다. 반면에 compound 48/80과 serotonin에 의한 혈관투과성 항진은 mouse 의 경우에서처럼 아답타겐에 의해 억제되었다.