• Mycobiology
• /
• 제49권5호
• /
• pp.461-468
• /
• 2021

The genus Laccaria (Hydnangiaceae, Agaricales) plays an important role in forest ecosystems as an ectomycorrhizal fungus, contributing to nutrient cycles through symbiosis with many types of trees. Though understanding Laccaria diversity and distribution patterns, as well as its association with host plants, is fundamental to constructing a balanced plant diversity and conducting effective forest management, previous studies have not been effective in accurately investigating, as they relied heavily on specimen collection alone. To investigate the true diversity and distribution pattern of Laccaria species and determine their host types, we used four different approaches: specimen-based analysis, open database search (ODS), NGS analysis, and species-specific PCR (SSP). As a result, 14 Laccaria species have been confirmed in Korea. Results regarding the species distribution pattern were different between specimen-based analysis and SSP. However, when both were integrated, the exact distribution pattern of each Laccaria species was determined. In addition, the SSP revealed that many Laccaria species have a wide range of host types. This study shows that using these four different approaches is useful in determining the diversity, distribution, and host of ECM fungi. Furthermore, results obtained for Laccaria will serve as a baseline to help understand the role of ECM fungi in forest management in response to climate change.

• 공업화학
• /
• 제9권3호
• /
• pp.342-347
• /
• 1998

경량성의 전도성복합수지는 전도성고분자인 폴리피롤과 폴리티오펜이 다공성의 가교폴리스티렌의 내부에 분산되어 전도성네트워크를 형성시킴으로 제조된다. 높은 다공성과 친유성의 특성을 갖는 호스트고분자는 고농축에멀션중합방법을 이용하여 합성하고 전도성복합수지는 이 호스트고분자를 이용하여 이단계 함침방법으로 제조한다. $FeCl_3$을 개시제와 혼입체로 사용하여 제조한 폴리피롤복합수지의 전기전도도는 0.82 S/cm이며, $FeCl_3$-아세토니트릴을 개시제로 사용하여 제조한 폴리티오펜복합수지는 전기전도도가 6.05 S/cm에 이른다. 전도성복합수지의 전기전도도는 사용된 산화제 용매와 초기산화제와 단량체 몰비율에 크게 영향을 받는다. 전자현미경조사에 의하면 전도성고분자가 다공성 호스트고분자 내부벽에 필림을 형성한다. 폴리피롤소재복합수지와 폴리티오펜소재복합수지의 전기차단효율은 2.0 GHz에서 각각 15.2dB와 22.5dB였다. 20K-300K의온도범위에서 저항의 온도의존성에 대한 실험결과에 의하면 전도성복합수지는 반도체특성을 보이고 전기전도기구는 전형적인 VRH모델을 따른다.

• Journal of Communications and Networks
• /
• 제7권3호
• /
• pp.325-336
• /
• 2005

Mobile ad hoc networks (MANET) are composed of moving wireless hosts which, within range of each other, form wireless networks. For communication to occur between hosts that are not within each other's range, routes involving intermediate nodes must be established; however, since the hosts may be in motion, a host that was part of a route may move away from its upstream and downstream partners, thus breaking the route. In this paper, we propose anticipated route maintenance (ARM) protocol with two extensions to route discovery based routing scheme: Extend the route when nodes on a link move apart from each other and they have common neighbor that can be 'inserted' in the path, and shrink route when a node discovers that one of its neighbor which is not the next hop is also on the same route several hops later on. By utilizing only local geographic information (now a part of some route finding algorithms), a host can anticipate its neighbor's departure and, if other hosts are available, choose a host to bridge the gap, keeping the path connected. We present a distributed algorithm that anticipates route failure and performs preventative route maintenance using location information to increase a route lifespan. The benefits are that this reduces the need to find new routes (which is very expensive) and prevents interruptions in service. As the density of nodes increases, the chance to successfully utilize our route maintenance approach increases, and so does the savings. We have compared the performance of two protocols, pure dynamic source routing (DSR) protocol and DSR with ARM. The simulation results show how ARM improves the functionality of DSR by preventing the links in the route from breaking. Packets delivery ratio could be increased using ARM and achieved approximately $\100%$ improvement. The simulations clarify also how ARM shows a noticeable improvement in dropped packets and links stability over DSR, even though there is more traffic and channel overhead in ARM.

• The Plant Pathology Journal
• /
• 제28권1호
• /
• pp.81-86
• /
• 2012

We developed a reassortant RNA virus vector derived from $Cucumber$ $mosaic$ $virus$ (CMV), which has advantages of very wide host range and can efficiently induce gene silencing in a few model plants. Certain CMV isolates, however, show limited host ranges presumably because they naturally co-evolved with their own hosts. We used a reassortant comprised of two strains of CMV, Y-CMV and Gn-CMV, to broaden the host range and to develop a virus vector for virus-induced gene silencing (VIGS). Gn-CMV could infect chili pepper and tomato more efficiently than Y-CMV. Gn-CMV RNA1, 3 and Y-CMV RNA2-A1 vector were newly reconstructed, and the transcript mixture of RNA1 and 3 genomes of Gn-CMV and RNA2 genome of Y-CMV RNA2 containing portions of the endogenous phytoene desaturase (PDS) gene (CMV2A1::PDSs) was inoculated onto chili pepper (cv. Chung-yang), tomato (cvs. Bloody butcher, Tigerella, Silvery fir tree, and Czech bush) and $Nicotiana$ $benthamiana$. All the tested plants infected by the reassortant CMV vector showed typical photo-bleaching phenotypes and reduced expression levels of $PDS$ mRNA. These results suggest that the reassortant CMV vector would be a useful tool for the rapid induction of the RNA silencing of endogenous genes in chili pepper and tomato plants.

• 한국응용곤충학회지
• /
• 제41권4호
• /
• pp.255-261
• /
• 2002

토마토녹응애(개칭)에 대해 주사전자현미경 관찰, 국내 분포, 기주식물, 온도에 따른 발육상, 약제 방제법에 관하여 연구하였다. 토마토녹응애는 2쌍의 다리를 가진 길이 $134.9\mu\textrm{m}$의 미소동물로 feathered claw는 4줄이었다. 피해증상으로, 피해엽은 적갈색으로 변하고 줄기에는 털이 없어지고 광택이 나거나 표면이 그물형으로 갈라지며, 새순은 시들거나 위축된다. 과일은 과경 부근에 그을음이나 녹이 긴 것 같이 되고, 그물형으로 갈라진다. 충남의 부여와 유성, 강원도 평창, 경북의 칠곡, 구룡포의 유리온실에서 발견되었으며, 한국에서 기주로는 메꽃, 까마중, 가지, 꽈리, 나팔꽃, 페투니아, 감자, 고추, 담배, 토마토 등이 확인되었다. 이 응애는 $15-30^{\circ}C$에서 발육이 가능하였고, 한 세대는 6-11일로 높은 온도일수록 발육기간이 짧아지는 경향이었으며, 암컷이 수컷보다 수명이 길었는데, 25-28$^{\circ}C$에서 12일로 가장 수명이 길었다. 살비제 중에서 fenazaquin과 cypermethrin의 효과가 가장 좋았다.

• The Plant Pathology Journal
• /
• 제38권4호
• /
• pp.383-394
• /
• 2022

In Japan, the P1 protein (S-type) encoded by leek yellow stripe virus (LYSV) isolates detected in Honshu and southward is shorter than the P1 (N-type) of LYSV isolates from garlic grown in Hokkaido due to a large deletion in the N-terminal half. In garlic fields in Hokkaido, two types of LYSV isolate with N- and S-type P1s are sometimes found in mixed infections. In this study, we confirmed that N- and S-type P1 sequences were present in the same plant and that they belong to different evolutionary phylogenetic groups. To investigate how LYSV with S-type P1 (LYSV-S) could have invaded LYSV with N-type P1 (LYSV-N)-infected garlic, we examined wild Allium spp. plants in Hokkaido and found that LYSV was almost undetectable. On the other hand, in Honshu, LYSV-S was detected at a high frequency in Allium spp. other than garlic, suggesting that the LYSV-S can infect a wider host range of Allium spp. compared to LYSV-N. Because P1 proteins of potyviruses have been reported to promote RNA silencing suppressor (RSS) activity of HC-Pro proteins, we analyzed whether the same was true for P1 of LYSV. In onion, contrary to expectation, the P1 protein itself had RSS activity. Moreover, the RSS activity of S-type P1 was considerably stronger than that of N-type P1, suggesting that LYSV P1 may be able to enhance its RSS activity when the deletion is in the N-terminal half and that acquiring S-type P1 may have enabled LYSV to expand its host range.

• Journal of Animal Science and Technology
• /
• 제65권3호
• /
• pp.652-663
• /
• 2023

The rumen fluids contain a wide range of bacteria, protozoa, fungi, and viruses. The various ruminal microorganisms in the rumen provide nutrients by fermenting the forage they eat. During metabolic processes, microorganisms present in the rumen release diverse vesicles during the fermentation process. Therefore, in this study, we confirmed the function of rumen extracellular vesicles (EVs) and their interaction with the host. We confirmed the structure of the rumen EVs by transmission electron microscope (TEM) and the size of the particles using nanoparticle tracking analysis (NTA). Rumen EVs range in size from 100 nm to 400 nm and are composed of microvesicles, microparticles, and ectosomes. Using the Caenorhabditis elegans smart animal model, we verified the interaction between the host and rumen EVs. Exposure of C. elegans to rumen EVs did not significantly enhance longevity, whereas exposure to the pathogenic bacteria Escherichia coli O157:H7 and Staphylococcus aureus significantly increased lifespan. Furthermore, transcriptome analysis showed gene expression alterations in C. elegans exposed to rumen EVs, with significant changes in the metabolic pathway, fatty acid degradation, and biosynthesis of cofactors. Our study describes the effect of rumen EV interactions with the host and provides novel insights for discovering biotherapeutic agents in the animal industry.

• Journal of Microbiology and Biotechnology
• /
• 제1권4호
• /
• pp.240-245
• /
• 1991

We have constructed a promoter-probe vector pKU20 using pKT230, a derivative of broad-host-range plsmid RSF1010, as a base. The pKU20 contains structural gene for aminoglycoside phos-photransferase (aph), without promoter, and a multiple cloning site upstream the aph. Using this vector, a 412base pairs (bp) PstI fragment showing strong promoter activity both in Escherichia coli LE392 and Pseudomonas putida KCTC1644 has been cloned from Pseudomonas fluorescens chromosomal DNA on the basis of streptomycin resistance. The nucleotide sequence of the 412 bp fragment has been determined and the putative - 35 and -10 region was observed. Insecticidal protein gene of Bacillus thuringiensis subsp. kurstaki HD-73 inserted on downstream of the promoterlike DNA fragment was efficiently expressed in E. coli and P. putida. The toxin protein was efficiently synthesized in an insoluble form in both strains.

• 식물병연구
• /
• 제17권2호
• /
• pp.232-234
• /
• 2011

During survey of postharvest diseases of vegetables in the middle region of Korea in 2003, 2004 and 2005, new disease symptoms showing watery rot and soft rot were observed. In this study, the disease causal agents were identified as Geotrichum candidum, and their host range and pathogenicity were investigated. G. candidum isolated had wide host range and strong pathogenicity against carrot, cucumber, tomato and pumpkin. The disease occurrence on several vegetables that G. candidum can be a serious threat to stable production of fresh vegetable.

• 식물병연구
• /
• 제19권2호
• /
• pp.121-123
• /
• 2013

사과, 호박, 고추에서 분리된 탄저병균들(Colletotrichum gloeosporioides, C. orbiculare, C. acutatum)이 복분자딸기에 병원성을 나타냈으며. 특히 사과에서 분리된 탄저병균은 무상처 접종에서도 병원성을 나타냈다. 복분자 딸기에서 분리한 탄저병균(Colletotrichum gloeosporioides, C. coccodes, C. acutatum)은 사과와 딸기에서는 dropping 접종에 의해 병원성을 보였으나 고추와 토마토에서는 낮은 병원성을 나타내었다.