• 한국균학회지
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• 제46권4호
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• pp.427-435
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• 2018

본 연구에서는 충남, 제주, 경남, 충북 등에서 새우난초(Calanthe discolor)와 은대난초(Cephalanthera longibracteata)의 뿌리를 채취하여 표면살균한 후 내생균을 순수분리 하였다. 분리된 내생균의 형태학 특징을 관찰하고 internaltranscribed spacer (ITS) rDNA영역의 염기서열을 ITS1F와 ITS4프라이머를 이용하여 분석하였다. 서로 다른 지역에서 채집한 새우난초와 은대난초이지만, 공통적으로 Leptodontidium orchidcola가 높은 상대수도와 빈도를 나타냈으며, 내생균류의 군집차이를 분석할 결과, 지역적 차이보다는 숙주에 의한 차이가 더 큰 영향을 미치는 것으로 나타났다.

• Mycobiology
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• 제39권4호
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• pp.249-256
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• 2011

Microorganisms are significantly affected when the ambient pH of their environment changes. They must therefore be able to sense and respond to these changes in order to survive. Previous investigators have studied various fungal species to define conserved pH-responsive signaling pathways. One of these pathways, known as the Pal/Rim pathway, is activated in response to alkaline pH signals, ultimately targeting the PacC/Rim101 transcription factor. Although the central signaling components are conserved among divergent filamentous and yeast-like fungi, there is some degree of signaling specificity between fungal species. This specificity exists primarily in the downstream transcriptional targets of this pathway, likely allowing differential adaptation to species-specific environmental niches. In this review, the role of the Pal/Rim pathway in fungal pH response is discussed. Also highlighted are functional differences present in this pathway among human fungal pathogens, differences that allow these specialized microorganisms to survive in the various micro-environments of the infected human host.

• 한국응용곤충학회지
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• 제53권1호
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• pp.39-49
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• 2014

한국산 무당벌레붙이과(Endomychidae Leach)를 분류학적으로 검토하였다. 한국산 무당벌레붙이과에는 3아과, 7속, 10종이 포함되어 있는데, 그 중 Endomychus gorhami (Lewis) Eumorphus quadriguttatus pulchripes Gerstaecker, Lycoperdina castaneipennis Gorham, Mycetina ancoriger Gorham 등 4종을 국내에서 처음으로 보고한다. 또한 Lycoperdina castaneipennis의 숙주버섯을 처음으로 보고한다.

• Mycobiology
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• 제45권4호
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• pp.312-317
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• 2017

Orchidaceous plants have symbiotic relationships with endophytic fungi, including mycorrhizal fungi, which play important roles in the seed germination and growth of the host plants. In this study, endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from three different sites in Korea were analyzed, and it was determined whether fungal communities were preferentially correlated with the sites. The fungal isolates were identified by sequence analysis of the internal transcribed spacer regions of rDNA. In total, 30 species of endophytic fungi, including two species of mycorrhizal fungi belonging to the genus Tulasnella, were identified. Leptodontidium orchidicola showed the highest frequency and was isolated from all root samples. Species diversity and richness were not significantly different among sites. However, the community structure of the endophytic fungi significantly differed among sites, suggesting that the site characteristics affected the community composition of the endophytic fungi colonizing the roots of C. longibracteata. Our findings will aid in developing methods involving the use of symbiotic fungi for orchid conservation and restoration in native habitats.

• Mycobiology
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• 제45권3호
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• pp.150-159
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• 2017

This study analyzed the distribution of endophytic fungi in 3 coastal environments with different climatic, geographical, and geological characteristics: the volcanic islands of Dokdo, the East Sea, and the West Sea of Korea. The isolated fungal endophytes were characterized and analyzed with respect to the characteristics of their host environments. For this purpose, we selected common native coastal halophyte communities from three regions. Molecular identification of the fungal endophytes showed clear differences among the sampling sites and halophyte host species. Isolates were also characterized by growth at specific salinities or pH gradients, with reference to previous geographical, geological, and climate studies. Unlike the East Sea or West Sea isolates, some Dokdo Islands isolates showed endurable traits with growth in high salinity, and many showed growth under extremely alkaline conditions. A smaller proportion of West Sea coast isolates tolerate compared to the East Sea or Dokdo Islands isolates. These results suggest that these unique fungal biota developed through a close interaction between the host halophyte and their environment, even within the same halophyte species. Therefore, this study proposes the application of specific fungal resources for restoring sand dunes and salt-damaged agricultural lands and industrialization of halophytic plants.

• Mycobiology
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• 제49권3호
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• pp.235-248
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• 2021

This study aimed to understand whether the geo-ecological segregation of native plant species affects the root-associated fungal community. Rhizoplane (RP) and rhizosphere (RS) fungal microbiota of Sedum takesimense native to three geographically segregated coastal regions (volcanic ocean islands) were analyzed using culture-independent methods: 568,507 quality sequences, 1399 operational taxonomic units, five phyla, and 181 genera were obtained. Across all regions, significant differences in the phyla distribution and ratio were confirmed. The Chao's richness value was greater for RS than for RP, and this variance coincided with the number of genera. In contrast, the dominance of specific genera in the RS (Simpson value) was lower than the RP at all sites. The taxonomic identity of most fungal species (95%) closely interacting with the common host plant was different. Meanwhile, a considerable number of RP only residing fungal genera were thought to have close interdependency on their host halophyte. Among these, Metarhizium was the sole genus common to all sites. These suggest that the relationship between potential symbiotic fungi and their host halophyte species evolved with a regional dependency, in the same halophyte species, and of the same natural habitat (volcanic islands); further, the fungal community differenced in distinct geographical regions. Importantly, geographical segregation should be accounted for in national culture collections, based on taxonomical uniqueness.

• 한국환경과학회지
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• 제23권8호
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• pp.1385-1394
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• 2014

Metcalfa pruinosa was considered to be a very harmful invasive species, due to its high species density in nature and wide range of its host plants. M. pruinosa was distributed in 28 sites among 143 sites. M. pruinosa has wide range and diverse host plants of 52 families 110 species including crop, fruits and forest trees. At present, the identified host plant of M. pruinosa are composed of 62 families and 145 species in total since their first appearance was reported. M. pruinosa was found in roadside 46.7%, followed by forests 33.3% and orchard 20%. Nymphs and adults cause damage to crop and orchard by sucking juice, outbreak of fungi through secretion of wax, and reduction of plant assimilation due to the nectar of nymphs. Also, it reduces the merchantable quality of fruits and thus causes economic damage. It is judged that M. pruinosa has been moved along major road via the traffic vehicles.

• 한국유기농업학회지
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• 제31권4호
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• pp.427-439
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• 2023

The silverleaf whitefly, Bemisia tabaci, is a major pest distributing worldwide damaging over 900 host plant species, and is highly resistant to chemical pesti- cides. Due to the high pesticide resistance of whitefly, there is a need for alternatives to chemical control. Entomopathogenic fungi are candidates for biological pesticide that can overcome the resistance problem of chemical pesticide. Therefore, in this study, we tested pathogenicity of the entomopathogenic fungi to select high insec- ticidal activity against whitefly. As a result, IPBL-C (Cordyceps fumosorosea) and IPBL-F (Metarhizium pinghaense) isolates showed high insecticidal activity against whitefly. Additionally, as a result of culturing the selected isolates on spent coffee grounds medium, the conidia of IPBL-F produced on coffee grounds medium showed five times higher heat stability after heat treatment at 45℃ for one hour than conidia produced on PDA medium.

• 한국균학회지
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• 제47권2호
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• pp.121-124
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• 2019

An endophytic fungus, Rhizosphaera pini strain NIE7426, was isolated from the sub-alpine coniferous tree Abies nephrolepis in Mt. Nochu of Gangwon Province. It was characterized by macroscopic and microscopic features, as well as the internal transcribed spacer (ITS) 1, 2 and 5.8S sequences. All morphological and molecular features support the first recognition of this taxon in Korea. In addition, this study adds A. nephrolepis as a host plant R. pini.

• 한국균학회지
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• 제39권1호
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• pp.1-6
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• 2011

본 연구에서는 수지상균근균(AMF)의 형태적 및 분자 생물학적 특징을 바탕으로 문안산에 분포하는 수지상균근균의 종 다양성을 분석하였다. AMF 시료는 생강나무(Lindera obtusiloba), 국수나무(Stephanandra incisa), 쪽동백(Styrax obassis), 노린재나무(Symplocos sawafutagi)의 뿌리와 뿌리 부근의 토양에서 채취하였으며, 수수(Sorghum bicolor)를 숙주식물로 사용하여 포자를 배양하였다. 추출된 포자들의 형태 형질과 염기서열을 분석한 결과 Acaulospora longula, A. mellea, Ambispora leptoticha, Gigaspora margarita, Paraglomus occultum 등 4속 5종이 동정되었으며, 숙주식물에 따른 수지상균근균의 분포는 수종에 따라서 다른 경향을 보였다. 생강나무에서는 A. longula가, 국수나무, 쪽동백나무, 노린재나무에서는 A. leptoticha가 우점종이었다.