• BMB Reports
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• 제38권4호
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• pp.414-419
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• 2005

The production of recombinant antibodies has been generally recognized as time-consuming and labor-intensive. The aim of our study is to construct mammalian expression vectors containing the cDNA encoding the human constant regions and murine variable regions to massively and cost-effectively produce full-length chimeric antibodies. Unique restriction sites flanking the Ig variable region were designed to allow for the replacement of variable regions generated by PCR. Western blot analysis of the chimeric antibodies revealed that the expressed products were of the predicted size, structure and specificity. The usefulness of the vectors was confirmed by construction of human-mouse chimeric antibody-HCAb which secretes murine antibody against the human colorectal cancer. Selected in medium containing gradually increasing methotrexate (MTX), clones with increased expression of the product gene can be efficiently generated. The secretion of recombinant chimeric antibody-HCAb yielded $30\;pg\;cell^{-1}\;day^{-1}$ at $10^{-6}\;M$ MTX. With this high-level expression from pools, the convenient and rapid production of over 100 milligram amounts per liter of recombinant antibodies may be achieved, which indicates the significant roles of pYR-GCEVH and pYR-GCEVL in the production of chimeric antibodies.

• 디지털융복합연구
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• 제10권7호
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• pp.193-200
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• 2012

본 연구는 2008년 환자조사 자료를 이용하여 위장질환과 충수염질환을 대상으로 지역간 입원의료이용의 변이정도와 양상을 파악하고, 변이 발생에 영향을 미치는 관련 요인을 분석한 후, 이를 기초로 변이를 최소화하여 의료 이용을 적정화 시킬 수 있는 방안을 모색하기 위해 수행하였다. Extremal Quotient(EQ)와 변이계수(CV) 지표를 사용하여 변이수준을 파악하였고, 다중회귀분석을 통해 입원의료이용의 차이에 영향을 미치는 주요 요인들을 분석하였다. 주요 연구결과는 다음과 같다. 첫째, 위장질환의 소규모 지역별 성 연령 표준화에 따른 입원율의 경우 EQ 15.1, CV 0.4의 변이를 보였으며, 특히 농어촌 지역에서 변이 10대 상위군이 높은 분포를 나타냈다. 또한, 인구만명당 병상수, 의사수, 특수의료장비수에 따라 입원의료이용량이 통계적으로 유의한 양의 관계를 보였다. 둘째, 충수염질환에서는 EQ 12.41, CV 0.4를 나타냈고, 회귀분석을 통해 입원의료이용의 차이에 영향을 미치는 요인을 분석한 결과 통계적으로 유의하지 않았다. 본 연구 결과의 정책적인 함의로는 급성질환자의 입원의료이용의 형평성을 제고할 수 있도록 지역별 병상의 적정한 공급 관리와 효율적인 자원배분 및 의료서비스의 질적 차이를 감소시킬 수 있는 다각적인 정책 방안이 추진되어야 함을 시사해 준다.

• 융합정보논문지
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• 제9권9호
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• pp.195-200
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• 2019

국민건강보험 청구자료는 급여 지급을 위한 자료로 질환에 대한 유병 및 발생자료는 아니므로 보건학적 연구 자료로 활용하기 위해서는 진료에피소드개념을 적용하여 재가공이 필요하다. 따라서 건강보험 청구자료의 보건학적 연구 자료로 활용하기 위한 시범적 진료에피소드 자료를 구축하는 것이 목적이다. 본 연구에서는 무진료기간을 퇴원한 당일 입원한 경우 월 단위 분리청구를 보정하기 위해 0으로 정의하였다. 서울지역에 한하여 호흡기계 질환(ICD10: J00-J99)및 심혈관계 질환(ICD10: I00-I99) 발생의 진료개시일부터 입원 이후 재입원까지 경향을 확인하였다. 무진료기간이 0일 일 때 자료 감소율은 건강보험청구자료의 월 단위 분리청구건으로 인한 것으로 판단된다. 또한 의료기간 종별에 따라 월 단위 분리청구 경향 차이가 있는 것으로 확인 하였다. 본 연구를 통해 구축된 시범적 진료에피소드자료는 기초적 역학정보를 산출하는 자료처리 기법으로 이용될 수 있을 것이다.

• 대한건축학회논문집:계획계
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• 제34권8호
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• pp.105-116
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• 2018

The architecture reflects geographical characteristics such as geographical environments and climate, it has the characteristics and cultural characteristics of the region that has been constructed. Hansen Village constructed in Period of Japanese Occupation at 'Sorok-Island' in Go-heung, Jeollanam-do was also based on this point. The village, which is a ward of Sorok-Island Province, consists of a village in which patients are self-sufficient in economic and social activities, unlike the modern hospital systems where patients and treatments are concentrated in a single room. Additionally, the facilities for patient acceptance were gradually expanded, with limited interaction with external. Sorok-island shows the expansion of the village center where the new village is formed with the increase of the number of the early adopters in the 1910s and the accommodation facility is built, and it gradually increases the necessary space by opening and repairing the existing facilities by the 1990s Change. In addition, the spatial composition of the village can be divided into three types : decentralized, centralized and single-shift ; it has the same causal relationship as the village's formation. The purpose of this study is identifying the uniqueness of Sorok-Island by analyzing the correlation between the formation of a village to accommodate Hansen Patients in Sorok-Island and the spatial Component Factor.

• 대한한의학방제학회지
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• 제30권4호
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• pp.339-349
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• 2022

Objectives : The purpose of this study is to report one case of Korean medical treatment of patient with developmental abnormality by cerebral palsy whose main symptoms are difference in left and right leg length, foot size and construction of right ankle. Methods : We did retrospective study for one developmental abnormality patient who had been treated by herbal medicine(Samul-tang, Yookmijihwang-tang hap Yijin-tang + Sungjang-hwan), acupuncture, and moxibustion for more than one year. The effects of treatment were evaluated by measurement of leg length, foot size and dorsiflexion ROM. Results : After treatment, length and foot size difference between both leg, ROM difference between both ankle were almost reduced. According to the ratio of the right to the left, leg length was improved from 0.91 to 0.98 and foot size was improved from 0.93 to 0.98. The difference in left and right ankle dorsiflexion ROM was improved from 55° to 27.5°. Conclusions : This clinical case study has demonstrated that Korean medicine may be an safe and effective option for treating developmental abnormality.

• Journal of Hospice and Palliative Care
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• 제24권4호
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• pp.226-234
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• 2021

Purpose: The accessibility of medical facilities for cancer patients affects both their comfort and survival. Patients in rural areas have a higher socioeconomic burden and are more vulnerable to emergency situations than urban dwellers. This study examined the feasibility and effectiveness of a cancer care model integrating a regional cancer center (RCC) and public health center (PHC). Methods: This study analyzed the construction of a safety care network for cancer patients that integrated an RCC and PHC. Two public health institutions (an RCC in Gyeongnam and a PHC in Geochang County) collaborated on the development of the community care model. The study lasted 13 months beginning in February 2019 to February 2020. Results: The RCC developed the protocol for evaluating and measuring 27 cancer-related symptoms, conducted education for PHC nurses, and administered case counseling. The staff at the PHC registered, evaluated, and routinely monitored patients through home visits. A smartphone application and regular video conferences were incorporated to facilitate mutual communication. In total, 177 patients (mean age: 70.9 years; men: 59%) were enrolled from February 2019 to February 2020. Patients' greatest unmet need was the presence of a nearby cancer treatment hospital (83%). In total, 28 (33%) and 44 (52%) participants answered that the care model was very helpful or helpful, respectively. Conclusion: We confirmed that a combined RCC-PHC program for cancer patients in rural areas is feasible and can bring satisfaction to patients as a safety care network. This program could mitigate health inequalities caused by accessibility issues.

• 가정간호학회지
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• 제30권1호
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• pp.48-58
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• 2023

Purpose: The purpose of this study was to construct a nomogram for predicting difficulty in peripheral intravenous cannulation (DPIVC) for adult inpatients. Methods: This study conducted a secondary analysis of data from the intravenous cannulation cohort by intravenous specialist nurses at a tertiary hospital in Seoul. Overall, 504 patients were included; of these, 166 (32.9%) patients with failed cannulation in the first intravenous cannulation attempt were included in the case group, while the remaining 338 patients were included in the control group. The nomogram was built with the identified risk factors using a multiple logistic regression analysis. The model performance was analyzed using the Hosmer-Lemeshow test, area under the curve (AUC), and calibration plot. Results: Five factors, including vein diameter, vein visibility, chronic kidney disease, diabetes, and chemotherapy, were risk factors of DPIVC. The nomogram showed good discrimination with an AUC of 0.81 (95% confidence interval: 0.80-0.82) by the sample data and 0.79 (95% confidence interval: 0.74-0.84) by bootstrapping validation. The Hosmer-Lemeshow goodness-of-fit test showed a p-value of 0.694, and the calibration curve of the nomogram showed high coherence between the predicted and actual probabilities of DPIVC. Conclusion: This nomogram can be used in clinical practice by nurses to predict DPIVC probability. Future studies are required, including those on factors possibly affecting intravenous cannulation.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제29권4호
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• pp.29-35
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• 2023

Purpose: The 2015 Middle East Respiratory Syndrome (MERS) outbreak and the recent COVID-19 pandemic have highlighted the lack of negative pressure isolation rooms and the fragility of the healthcare system. The need for healthcare facility transformation for respiratory infectious diseases has become more prominent due to COVID-19, and the purpose of this study is to provide a foundation for the rapid, economical, and safe construction of negative pressure isolation wards. Methods: This study analyzes the current status of hospitals that have been converted to negative pressure isolation rooms, and provides architectural plans and examples to provide a reference for bedroom change. Research data of this study have been obtained by analyzing the drawings of negative pressure isolation wards of nationally designated inpatient treatment beds and urgent isolation beds. In addition, the relevant literature of urgent isolation beds has been analyzed to derive bedroom change type. Result: In this study, a total of 21 isolation bed conversion methods have been presented. Implications: In order to change efficiently from a general ward to an isolation ward, it is necessary to consider the actual hospital's infectious disease transmission patterns and facility conditions.

• 대한치매학회지
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• 제23권1호
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• pp.44-53
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• 2024

Background and purpose: The anti-aging standard forest healing program (ASFHP), which uses forest therapy, was reported to be effective in improving psychological, physical, and cognitive functions. However, there are several challenges to directly visiting the forest. This study aimed to investigate the impact of multi-session ASFHP with forest visit on the mental and physical health of the older people with visits to forest facilities and compared them with those of the same program conducted indoors. Methods: Individuals aged over 70 years with concerns about cognitive decline were recruited at dementia relief centers and divided into control and experimental groups. A total of 33 people were administered ASFHP under the supervision of a forest therapy instructor. The control group stayed indoors, while the experimental group visited a forest healing center and repeated the program 20 weeks. Results: The multiple-session ASFHP positively affected cognitive impairment screening test (CIST) total scores (p=0.002), memory (p=0.014), Korean version of the Repeatable Battery for the Assessment of Neuropsychological Status total scores (p<0.001), immediate recall (p=0.001), visuospatial/construction (p<0.001), language (p<0.001), forest healing standard questionnaire total scores (p=0.002), and cognitive function (p=0.019), regardless of location. The forest visits during the ASFHP showed positive effects on orientation (p=0.035), delayed recall (p=0.042), emotional stability (p=0.032), physical activity (p=0.005), and health (p=0.022). The CIST scores of the memory domain were the strongest indicator of the multiple-session ASFHP effects. Conclusions: The 20-week multi-session ASFHP with forest visit showed effects on cognitive improvement and physical and emotional stability compared to indoor education.

• 한국조경학회지
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• 제47권5호
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• pp.14-27
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• 2019

본 연구는 5.18민주화운동 이후 사적지로 지정되었던 국군광주병원과 505보안부대 옛터를 대상으로 역사적 사실을 바탕으로 한 시대적 특성을 공간설계 개념으로 설정해 기념공원화 계획안을 제시하였다. 광주광역시는 5.18사적지인 국군광주병원과 505보안부대 옛터를 5.18기념사업의 일환으로 공원화하고자 도시공원 조성계획을 수립하고, 역사에 대한 기억의 공간으로만 보존해왔던 대상지를 5.18민주화운동의 역사적 가치를 함양함과 동시에 시민 삶에 활력을 주는 공원으로 단계적 조성계획을 추진하고 있다. 결과적으로 5.18사적지의 공동체적 가치가 현대적으로 계승될 수 있도록 사회적 합의를 통해 단계별 계획, 조성과정의 틀을 설정하고, 상처의 치유, 역사의 기억을 담아내는 계획을 하고자 함이다. 계획안에서 사적지의 공원화와 관련된 선행연구 및 계획사례 분석을 통해 공간 활용을 위한 문제 해결적 접근방법을 제시하였고, 대상지가 갖고 있는 장소적 가치를 탐색함과 동시에 공원의 계획개념을 유지하면서 역사적 사실에 대한 시대적 특성을 담아내는 공간 활용 전략과 세부내용을 서술하였다. 연구의 결과로 국군광주병원 사적지는 5.18민주화운동을 통해 남겨진 상처에 대한 치유의 문제를 지역사회가 주체적으로 해결할 수 있도록 과거의 역사적 기억과 치유의 공원으로 계획하되, 기존 자연환경을 활용한 치유의 숲 개념을 제시하였고, 505보안부대 옛터 사적지는 5.18기억공동체의 확장과 지속성을 강화하는 측면에서 미래 세대인 어린이와 청소년이 공감할 수 있는 역사 체험 배움의 공간개념으로 계획하였다. 사적지를 활용한 기념공원화 계획은 계획 단계에서 시민, 관련단체, 전문가를 아우르는 사회적 공감대의 형성이 선결요건으로 작용하며, 장소성 재현을 위한 구성원들의 사회적 합의가 공간활용 방향 설정에 중요한 계획적 요소로 작용할 수 있다. 본 연구를 통해 5.18사적지에 역사적 가치를 담는 기념공원으로 환원하는 데 기여하고, 과거 역사에 대한 상처와 기억을 치유하는 장소를 넘어 도시와 시민의 삶에 활력을 주며, 미래 세대와의 공유할 수 있는 방향성을 제시한 것에 대한 의의가 있다.