• Korean journal of applied entomology
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• v.63 no.1
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• pp.13-23
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• 2024

Vespa velutina nigrithorax du Buysson, 1905 is the invaded species in Korea since 2003. Since its importance as the honey bee pest, beekeepers use insecticides to kill the adult and immature hornets. However, its legality and effectiveness has not been confirmed. This study investigated the susceptibility of insecticides commonly used to control hornets by beekeepers in Korea. Eight insecticides were tested on adult worker and larvae by topical or oral treatment. Adults showed more than 70% mortalities from Clothianidin, Dinotefuran, and Carbosulfan treatment within 30 minutes. Bifenthrin and Cartap hydrochloride showed relatively low toxicity. The median lethal dose (LD₅₀) for Clothianidin, Dinotefuran, and Carbosulfan was 0.29, 0.65, and 2.21 ㎍/bee, respectively. In larval feeding test where 5^th instar larvae were fed 3 times every 24 hours, the mortality began after second treatments. After 3rd treatments (72 h), all insecticides showed mortality more than 70%. The LD₅₀ values of Clothianidin, Dinotefuran, and Carbosulfan to V. velutina were approximately 10 to 100 times higher than those to honey bee, Apis mellifera. This study provides the basic information of those chemical toxicities to Vespa hornet and honey bees.

• Analytical Science and Technology
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• v.32 no.6
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• pp.252-261
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• 2019

Pyrrolizidine alkaloids (PAs) are a group of secondary metabolites that are produced by plants all over the world as a defense mechanism against herbivores. To date, over 660 PAs have been identified from more than 6,000 plant species that have been reported to be widely present in plants belonging to Asteraceae, Boraginaceae, and Fabaceae. This study describes an analytical method based on UPLC-MS/MS for the quantitation of 7 pyrrolizidine alkaloids (Lycopsamine, Echimidine, Retrorsine, Retrorsine N-oxide, Senecionine, Heliotrine, and Trichodesmine) in honey, and was applied to 84 honey samples for validation. Quantitation was performed based on a matrix-matched calibration to compensate for the matrix effect on the electrospray ionization. Good linear calibrations were obtained for all 7 PAs in the spiked honey samples (2.575-202.14 ㎍/kg; R² ≥ 0.998). The extraction recoveries for most of the PAs in the honey samples were in the range of 81 %-108 %. The analysis showed that 6 of the 84 honey samples were contaminated by the PAs with the mean total sum of PAs being 47.19 ㎍/kg, and the concentrations of the PAs were observed to be in the range of 1.76-202.1 ㎍/kg. The retronecine type compounds (Echimidine, Lycopsamine) were the most frequently found PAs in honey. These data provide useful information for the assessment of human risk posed by the consumption of honey contaminated PAs.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.291-294
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• 2008

Cymbidium 'Honey Girl' was developed in 2005 by the National Horticultural Research Institute, Rural Development Administration. This cultivar came from cross between Cym. 'Yakoto' and Cym. 'Marian Simplicity' in 1995. Fifty seven seedlings were obtained after planting and acclimatization in green house. In 1999, a line was selected by its performance such as flower color, leaf shape, flower stalk, and vigorous growth which was named 'Wongyo F1-13'. The line code '9521928'($V_2$) had uniformity and excellent characteristics. The selected line was named as 'Honey Girl' after second characteristics test. This cultivar had white color ('WN 155B') and medium sized leaves and flowers (average leaf length is about 57 cm and width of flower is about 7.7cm) with many flowers and erect flower stalks. Blooming begins from the end of November to December under the normal culture conditions.

• Korean journal of applied entomology
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• v.57 no.3
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• pp.233-233
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• 2018

• Journal of Life Science
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• v.28 no.12
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• pp.1489-1500
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• 2018

Honey is made from flower nectar by honey bees. In this study, 120 honeys from various flowers and across eight different provinces in Korea were collected and their components, antioxidants, and hemolytic activities against red blood cell were evaluated. Our results show that total polyphenol (TP) varied widely across the samples, with chestnut honey showing the highest TP ($77.1{\pm}8.4mg/100g$), protein content ($25.9{\pm}0.9mg/100g$), and absorbance at 400 nm ($A_{400}$ : $0.156{\pm}0.036$). In contrast, the acacia honey and sugar honey had a TP of 9.5~30 mg, 12~15 mg/100g of, and the lowest $A_{400}$ of $0.06{\pm}0.02$. High amounts of total flavonoid were quantified in the jujube and chestnut honeys at $8.73{\pm}7.31$ and $8.39{\pm}3.02mg/100g$, respectively. No samples demonstrated hemolytic activity up to 1 mg/ml. Antioxidant activities determined by DPPH, ABTS, and nitrite scavenging placed the chestnut honey highest, followed by jujube, styrax, multi-floral, citrus, acacia and sugar honey. Analysis of parameter correlations indicated that the components and bioactivity of the honey are dependent on the origin of the flower rather than on bee-farming regions. A positive correlation between TP content and antioxidant activity was identified. The correlation coefficients between $A_{400}$ and the TP, ABTS scavenging, and reducing power values were 0.804, 0.772 and 0.741, respectively. We therefore suggest that $A_{400}$ could be used as a noble, economic and simple factor for honey quality evaluation. Our results can potentially be used to develop functional honey for the food and pharmaceutical industries.

• Journal of Apiculture
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• v.33 no.4
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• pp.297-301
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• 2018

Hawthorn is widely distributed in Korea and has been used as herbal medicine for treating various cardiovascular disease, arteriosclerosis and hypertension in Korea. In order to select superior honey tree plant from Korea, the free sugar and organic acid in hawthorn fruits, including five Korean clones and four Chinese cultivars, were evaluated. We also compared these hawthorn fruits of five clones (selected from different area of Korea) with Chinese hawthorn cultivars. Glucose, galactose, fructose and sucrose were the major sugar components of hawthorn. In this study, we observed that sucrose, glucose and fructose content. The highest sucrose content of hawthorn fruit was 188.12g/100g in Daegeumseong cultivar. The sweetness index, which plays important role of taste, was also calculated from the content of sucrose, glucose and fructose. The contribution of each carbohydrate was calculated, based on the fact that fructose is 2.30 and sucrose 1.35 times sweeter than glucose. The highest sweetness of hawthorn fruit was 579.52 in Pocheon clone. Main organic acid detected in hawthorn fruit were citric acid, malic acid and shikimic acid. The highest citric acid and malic acid content in hawthorn fruit were 157.50g/100g (Pocheon 3) and 34.12g/100g (Daegeumseong), respectively. The results of this study would be helpful for using food and functional food products, due to the beneficial effects of free sugar and organic acid for human health such as antioxidants and anticarcinogenic properties.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.340-343
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• 2008

A new pale yellow gerbera(Gerbera hybrida Hort.) cultivar 'Honey Cream' was developed from a cross between 'Princessa' and 'Picasso', followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) in 2006. Three times evaluations were conducted from 2003 for the detailed characteristics of the new cultivar. 'Honey Cream' has semi-double type large flowers in good harmony with pale yellow(RHS, 11-D) ray floret and green center. It has good, stable flower shape and strong peduncle, and its vase life was 12.3 days. The average flower yield of 'Honey Cream' was about 48.5 stems per plant/year in greenhouse trails during 2003 and 2006. This cultivar was registered to the Korea Seed and Variety Service(KSVS) for commercialization in 2007. Year-round production of this cultivar is possible in the greenhouse condition in Korean climate.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.1
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• pp.1-12
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• 2022

Tilia amurensis is an important honey plant. As T. amurensis mainly distributes mountainous area with various elevations in Korea, accurate prediction of blooming time at the different elevation would benefit forest beekeepers. In this study, we measured time-dependent blooming progress of T. amurensis in Mt. Gariwang area ranging from 500-1500m. Additionally we collected blooming data from web and published literatures and estimated the variation of blooming time relative to the geographic locations. Flowers began to bloom from July 6 to July 22 with full blooming on July 14 in location where elevation is 638m in Mt. G ariwang area in 2021. Based on these databases, a growing degree day (G DD) model was developed for prediction of T. amurensis blooming progress using average daily temperatures. Using the starting date of G DD accumulation of January 1 and base temperature of 5 ℃, blooming period ranging from 10% to 90% of cumulative blooming rate was estimated as 860-1198 degree days (DD). This corresponded to the beginning to the end of July in Mt. Gariwaning area in 2021. This model could explain the phenological variations of T. amurensis flower blooming possibly affected by elevation within geographic area, latitude or year relative to the climate change, and aid forest beekeepers for better timing of nectar foraging by honey bees.

• Korean Journal of Food Science and Technology
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• v.44 no.4
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• pp.401-410
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• 2012

This study examines the authenticity discrimination of the circulated honey by using stable isotope ratio methods. In the case of domestic honey, the range of ${\delta}^{13}C$ for the samples labeled as pure honey was about -27- -21‰ at the $C_3$ origin, and the range of that for artificial honey was over -19‰ at the $C_4$ origin. The range of ${\delta}^{13}C$ for all imported honey was over -27- -23‰ originating from the $C_3$ plant. According to the nectar-source, ${\delta}^2H$ and ${\delta}^{18}O$ for domestic honey were significantly different for 6 and 5 groups, respectively. However, we could not explain the detailed relationship as well as the geographical feature of ${\delta}^2H$ and ${\delta}^{18}O$. The difference for ${\delta}^2H$ and ${\delta}^{18}O$ in the wide range of latitude, such as between Australia and Canada, was more or less shown. However, it was difficult to find out the trends of ${\delta}^2H$ and ${\delta}^{18}O$ for imported honey versus the geographical information in the similar latitudinal country.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.1
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• pp.29-38
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• 2012

This study was carried out to compare the agronomic characteristics, forage yield, and feed value of nine sorghum ${\times}$ sudangrass hybrids at paddy field cultivation. The nine recommended sorghum ${\times}$ sudangrass hybrids used in this study were P877F, Cow pow, Turbo gold, Maxi graze, Jumbo, G7, Sweet home, Honey chew BMR and Green star. Plant height, leaf number, stem diameter, and stem hardness were higher in Jumbo than other varieties (P<0.01). Sugar content (brix) was higher in order of P877F > Sweet home > maxi graze > Green star > Cow pow > G7 (P<0.01). Fresh (86,199kg/ha) and dry matter yield (16,206 kg/ha) of Jumbo were higher than other varieties (P<0.01). Crude protein was the highest in Maxi graze (6.5%), but crude fat was the highest in Honey chew BMR as 2.1% (P<0.01). NDF and ADF of Jumbo and G7 were higher than other varieties (P<0.01). TDN was higher in order of Maxi graze > Sweet home > P877F > Honey chew BMR > Green star, but no significant differences were found among the varieties. Minerals were the highest in Cow pow (15,020.5 mg/kg), and Sweet home (6,222.6 mg/kg) was the lowest as compared to other varieties (P<0.01). Total amino acids were higher in order of Maxi graze > Sweet home > Turbo gold > Honey chew BMR > Jumbo (P<0.01). Crude protein yield and crude fat yield were the highest in Turbo gold (814.6 kg/ha) and Honey chew BMR (309.8kg/ha), respectively (P<0.01). Mineral yield was the highest in Cow pow as 207.6 kg/ha (P<0.01). Amino acid yield (592.2 kg/ha) and TDN yield (10,194 kg/ha) were the highest in Sweet home. Based on the above results, sorghum ${\times}$ sudangrass hybrid varieties should be selected on the basis of the purpose of use. Because, each varieties showed various growth characteristics and nutrition yield (protein, fat, mineral, amino acid, TDN).