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http://dx.doi.org/10.5806/AST.2019.32.6.252

Characterization and screening of pyrrolizidine alkaloids by UPLC-MS/MS: Application to honey  

Ryu, Hoejin (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Oukhee (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Lee, Eunsoon (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Misun (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Jeong-gon (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Yun, Eunsun (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Hyunjeong (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Musang (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Publication Information
Analytical Science and Technology / v.32, no.6, 2019 , pp. 252-261 More about this Journal
Abstract
Pyrrolizidine alkaloids (PAs) are a group of secondary metabolites that are produced by plants all over the world as a defense mechanism against herbivores. To date, over 660 PAs have been identified from more than 6,000 plant species that have been reported to be widely present in plants belonging to Asteraceae, Boraginaceae, and Fabaceae. This study describes an analytical method based on UPLC-MS/MS for the quantitation of 7 pyrrolizidine alkaloids (Lycopsamine, Echimidine, Retrorsine, Retrorsine N-oxide, Senecionine, Heliotrine, and Trichodesmine) in honey, and was applied to 84 honey samples for validation. Quantitation was performed based on a matrix-matched calibration to compensate for the matrix effect on the electrospray ionization. Good linear calibrations were obtained for all 7 PAs in the spiked honey samples (2.575-202.14 ㎍/kg; R2 ≥ 0.998). The extraction recoveries for most of the PAs in the honey samples were in the range of 81 %-108 %. The analysis showed that 6 of the 84 honey samples were contaminated by the PAs with the mean total sum of PAs being 47.19 ㎍/kg, and the concentrations of the PAs were observed to be in the range of 1.76-202.1 ㎍/kg. The retronecine type compounds (Echimidine, Lycopsamine) were the most frequently found PAs in honey. These data provide useful information for the assessment of human risk posed by the consumption of honey contaminated PAs.
Keywords
Pyrrolizidine alkaloids (PAs); honey; UPLC-MS/MS; Lycopsamine; Echimidine;
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  • Reference
1 B. Kmellar, P. Fodor, L. Pareja, C. Ferrer, M. A. Martínez- Uroz, A. Valverde, and A. R. Fernandez-Alba, Journal of Chromatography A, 1215(1), 37-50 (2008).   DOI
2 B. Avula, S. Sagi, Y.-H. Wang, J. Zweigenbaum, M. Wang, and I. A. Khan, Food Chemistry, 178, 136-148 (2015).   DOI
3 Y. Zhou, N. Li, F. F.-K. Choi, C.-F. Qiao, J.-Z. Song, S.-L. Li, X. Liu, Z.-W. Cai, P. P. Fu, G. Lin, and H.-X. Xu, Analytica Chemical Acta, 681, 33-40 (2010).   DOI
4 L. Zhu, Z. Wang, L. Wong, Y. He, Z. Zhao, Y. Ye, P. P. Fu, and G. Lin, Food Control., 85(1), 484-494 (2018).   DOI
5 C. T. Griffin, S. M. Mitrovic, M. Danaher, and A. Furey, Food Additives & Contaminants: Part A, 32(2), 214-228 (2015).   DOI
6 P. P. J. Mulder, P. López, M. Castelari, D. Bodi, S. Ronczka, A. Preiss-Weigert, and A. These, Food Additives & Contaminants: Part A, 35(1), 118-133 (2018).   DOI
7 European Food Safety Authority (EFSA), The EFSA Journal, 447(1), 1-51 (2007).
8 European Food Safety Authority (EFSA), The EFSA Journal, 9(11), 1-146 (2011).
9 P. P. Fu, Q. Xia, M. W. Chou, and G. Lin, Journal of Food and Drug Analysis, 15(4), 400-415 (2007).
10 Y. Jiang, P. P. Fu, and G. Lin, Asian Journal of Pharmacodynamics and Pharmacokinetics, 6(3), 187-192 (2006).
11 M. Dreger, M. Stanislawska, A. Krajewska-Patan, S. Mielcarek, P.L. Mikolajczak, and W. Buchwald, Journal Herba Polonica, 55(4), 127-147 (2009).
12 L. A. Hoogenboom, P. P. Mulder, M. J. Zeilmaker, H. J. van den Top, G. J. Remmelink, E. F. Brandon, M. Klijnstra, G. A. Meijer, R. Schothorst, and H. P. Van Eqmond, Food Additive and Contaminants, 28(3), 359-372 (2011).   DOI
13 H. Wiedenfeld, Journal of Marmara University Institute of Health Sciences, 1(2), 79-87 (2011).
14 Centre for Food Safety assessment, https://www.cfs.gov.hk/english/programme/programme_rafs/files/Pyrrolizidine_Alkaloids_in_Food_e.pdf, Assessed 9 Aug 2019.
15 Dutch National Institute for Public Health and the Environment (RIVM), Advisory report on pyrrolizidine alkaloids in herbal preparations, 2015.
16 J. Rossmann, S. Schubert, R. Gurke, R. Oertel, and W. Kirch, Journal of Chromatography B, 969(1), 162-170 (2014).   DOI
17 A. Cappiello, G. Famiglini, P. Palma, and H. Trufelli, Journal of Liquid Chromatography & Related Technologies, 33(1), 1067-1081 (2010).   DOI