• Molecules and Cells
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• v.23 no.3
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• pp.357-362
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• 2007

There is an increasing demand for high throughput (HTP) methods for gene analysis on a genome-wide scale. However, the current repertoire of HTP detection methodologies allows only a limited range of cellular phenotypes to be studied. We have constructed two HTP-optimized expression vectors generated from the red fluorescent reporter protein (RFP) gene. These vectors produce RFP-tagged target proteins in a multiple expression system using gateway cloning technology (GCT). The RFP tag was fused with the cloned genes, thereby allowing us localize the expressed proteins in mammalian cells. The effectiveness of the vectors was evaluated using an HTP-screening system. Sixty representative human C2 domains were tagged with RFP and overexpressed in HiB5 neuronal progenitor cells, and we studied in detail two C2 domains that promoted the neuronal differentiation of HiB5 cells. Our results show that the two vectors developed in this study are useful for functional gene analysis using an HTP-screening system on a genome-wide scale.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.3
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• pp.22-30
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• 2010

This paper proposes a combination system of Adaptive Modulation and Coding (AMC) and Multiple Input Multiple Output (MIMO), which improves the throughput and has a better reliability. In addition, the system includes Precoding, Antenna Subset Selection and MIMO Mode Selection scheme. Finally, we make a performance analysis of the proposed system. The principal environmental parameters for the simulation experiment consist of a frequency non-selective rayleigh fading channel and a Spreading Factor (SF) of 16. Other parameters may be included in order to fulfill the requirements of the HSDP A Standard. The proposed system has a higher throughput and more reliability than the conventional system, which does not include MIMO Mode Selection scheme, Precoding or Antenna Subset Selection. According to the simulation results, the proposed system reaches the maximum throughput at 8dB, presentlng an improvement of 6dB and twice higher throughput, respect to the conventional system. Specifically, at the point of -6dB, the conventional system reaches 2.5Mbps, while the proposed system reaches 6.4Mbps at the same SNR. Also, at the point of 2dB, each system reaches 7.5Mbps (conventional system) and 15.3Mbps (proposed system), with near twice the difference. According to the results exposed above, we can conclude that the system proposed in this paper has, as the greatest contribution, the improvement of the throughput, especially, the average throughput.

• The KIPS Transactions:PartC
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• v.8C no.6
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• pp.793-805
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• 2001

In the recent priority system, high-priority packet will be served first and low-priority packet will be served when there isn\`t any high-priority packet in the system. By the way, even high-priority packet can be blocked by HOL (Head of Line) contention in the input queueing System. Therefore, the whole switching performance can be improved by serving low-priority packet even though high-priority packet is blocked. In this paper, we study the performance of preemptive priority in an input queueing switch for high speed switch system. The analysis of this switching system is taken into account of the influence of priority scheduling and the window scheme for head-of-line contention. We derive queue length distribution, delay and maximum throughput for the switching system based on these control schemes. Because of the service dependencies between inputs, an exact analysis of this switching system is intractable. Consequently, we provide an approximate analysis based on some independence assumption and the flow conservation rule. We use an equivalent queueing system to estimate the service capability seen by each input. In case of the preemptive priority policy without considering a window scheme, we extend the approximation technique used by Chen and Guerin [1] to obtain more accurate results. Moreover, we also propose newly a window scheme that is appropriate for the preemptive priority switching system in view of implementation and operation. It can improve the total system throughput and delay performance of low priority packets. We also analyze this window scheme using an equivalent queueing system and compare the performance results with that without the window scheme. Numerical results are compared with simulations.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.4
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• pp.1292-1306
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• 2014

This paper proposes a space-polarization division multiple access (SPDMA) system that has limited feedback channels. The system simultaneously serves data streams to multiple mobile users through dual-polarized antenna arrays, by using pre-determined sets of precoding vectors that are orthogonal in both space and polarization domains. To this end, a codebook whose elements are sets of the precoding vectors is systematically designed based on the discrete Fourier transform (DFT) matrix and considering the power imbalance of polarized channels. Throughput of the SPDMA system is evaluated and compared to that of space division multiple access (SDMA) system, according to the various parameters including cross polarization discrimination (XPD). The results show that the throughput of SPDMA system outperforms that of SDMA in the environments of high XPD with many mobile users.

• Biomolecules & Therapeutics
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• v.22 no.4
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• pp.355-362
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• 2014

We have developed a fully automated high throughput drug screening (HTDS) system based on the microfluidic cell culture array to perform combinational chemotherapy. This system has 64 individually addressable cell culture chambers where the sequential combinatorial concentrations of two different drugs can be generated by two microfluidic diffusive mixers. Each diffusive mixer has two integrated micropumps connected to the media and the drug reservoirs respectively for generating the desired combination without the need for any extra equipment to perfuse the solution such as syringe pumps. The cell array is periodically exposed to the drug combination with the programmed LabVIEW system during a couple of days without extra handling after seeding the cells into the microfluidic device and also, this device does not require the continuous generation of solutions compared to the previous systems. Therefore, the total amount of drug being consumed per experiment is less than a few hundred micro liters in each reservoir. The utility of this system is demonstrated through investigating the viability of the prostate cancer PC3 cell line with the combinational treatments of curcumin and tumor necrosis factor-alpha related apoptosis inducing ligand (TRAIL). Our results suggest that the system can be used for screening and optimizing drug combination with a small amount of reagent for combinatorial chemotherapy against cancer cells.

• Nuclear Engineering and Technology
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• v.51 no.3
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• pp.908-914
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• 2019

Fusion power shutdown system (FPSS) is a safety system to stop plasma in case of accidents or incidents. The gas injection system for the FPSS presented in this work is designed to research the flow development in a closed system. As the efficiency of the system is a crucial property, plenty of experiments are executed to get optimum parameters. In this system, the flow is driven by the pressure difference between a gas storage tank and a vacuum vessel with a source pressure. The idea is based on a constant volume system without extra source gases to guarantee rapid response and high throughput. Among them, valves and gas species are studied because their properties could influence the velocity of the fluid field. Then source pressures and volumes are emphasized to investigate the volume flow rate of the injection. The source pressure has a considerable effect on the injected volume. From the data, proper parameters are extracted to achieve the best performance of the FPSS. Finally, experimental results are used as a quantitative benchmark for simulations which can add our understanding of the inner gas flow in the pipeline. In generally, there is a good consistency and the obtained correlations will be applied in further study and design for the FPSS.

• Proceedings of the IEEK Conference
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• 2000.11b
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• pp.144-147
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• 2000

Data security is an important issue in today's computer networks. In order to construct a safe infra in the open communication network, a cryptography is necessarily applied to several communication application fields like a high-speed networking system supporting real-time operation. A cryptography which has already realized by a software is designed by using a hardware to improve a throughput. In this paper, we design hardware architecture of IDEA by using a single iterative round method to improve a encryption throughput. In addition, we intend to develop a hardware design methodology that a specific cryptography operate with high-speed. The hardware model is described in VHDL and synthesized by the Samsung KG 80 Library in the Synopsys development software tool. With a system clock frequency 20MHz, this hardware permits a data conversion rate of more than 116 Mbit/s.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2008.10a
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• pp.894-897
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• 2008

This paper analyzes the throughput performance of a selective repeat (SR)/automatic repeat request (ARQ) scheme to transmit packet data in burst home network channel. To combat the high degree of error caused by transmission of home network data a robust error control scheme is a necessity. Basically, error control schemes can be divided in two categories: ARQ schemes and forward error correction (FEC) schemes. ARQ schemes are often used for reliable data transmission. The performance of packet transmission using SR-ARQ schemes for bursty channels is analyzed and simple analytical expressions of its throughput are presented. Theoretic analysis and numerical results indicate that a small number of packet sizes can get good performance in bursty home network channel.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.16 no.3
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• pp.175-180
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• 2003

As the integrated circuit device shrinks to the deep submicron regime, the ion implantation process with high ion dose has been attracted beyond the conventional ion implantation technology. In particular, for the case of boron ion implantation with low energy and high dose, the stabilization and throughput of semiconductor chip manufacturing are decreasing because of trouble due to the machine conditions and beam turning of ion implanter system. In this paper, we focused to the improved characteristics of processing conditions of ion implantation equipment through the modified deceleration mode. Thus, our modified recipe with low energy and high ion dose can be directly apply in the semiconductor manufacturing process without any degradation of stability and throughput.

• Korean Journal of Agricultural Science
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• v.45 no.3
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• pp.317-323
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• 2018

The demand for crop production is increasingly becoming steeper due to the rapid population growth. As a result, breeding cycles should be faster than ever before. However, the current breeding methods cannot meet this requirement because traditional phenotyping methods lag far behind even though genotyping methods have been drastically developed with the advent of next-generation sequencing technology over a short period of time. Consequently, phenotyping has become a bottleneck in large-scale genomics-based plant breeding studies. Recently, however, phenomics, a new discipline involving the characterization of a full set of phenotypes in a given species, has emerged as an alternative technology to come up with exponentially increasing genomic data in plant breeding programs. There are many advantages for using new technologies in phenomics. Yet, the necessity of diverse man power and huge funding for cutting-edge equipment prevent many researchers who are interested in this area from adopting this new technique in their research programs. Currently, only a limited number of groups mostly in developed countries have initiated phenomic studies using high throughput methods. In this short article, we describe the strategies to compete with those advanced groups using limited resources in developing countries, followed by a brief introduction of high throughput phenotyping.