• Journal of Microbiology and Biotechnology
• /
• 제19권5호
• /
• pp.474-481
• /
• 2009

A novel microorganism, designated as LG12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. An electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16S rRNA homology showed that the LG12 strain belongs to Rhodococcus erythropolis. R. erythropolis LG12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). In addition, R. erythropolis LG12 exhibited the highest acrylic acid-degrading activity among the tested microorganisms, including R. rhodochrous, R. equi, R. rubber, Candida rugosa, and Bacillus cereus. The effect of the culture conditions of R. erythropo/is LG12 on the production of 3-hydroxypropionic acid (3HP) from acrylic acid was also examined. To enhance the production of 3HP, acrylic acid-assimilating activity was induced by adding 1 mM acrylic acid to the culture medium when the cell density reached an $OD_{600}$ of 5. Further cultivation of R. erythropo/is LG 12 with 40 g/l of acrylic acid resulted in the production of 17.5 g/l of 3HP with a molar conversion yield of 44% and productivity of 0.22 g/l/h at $30^{\circ}C$ after 72 h.

• Journal of Microbiology and Biotechnology
• /
• 제12권6호
• /
• pp.962-971
• /
• 2002

The current study proposes a simple monodimensional model to estimate the linear growth rate of photosynthetic microorganisms in flat-plate photobioreactors (FPPBRs) during batch cultivation. As a model microorganism, Chlorella kessleri was cultivated photoautotrophically in FPPBRs using light-emitting diodes (LEDs) as the light sources to provide unidirectional irradiation in the photobioreactors. Various conditions were simulated by adjusting both the intensity of the light and the height of the culture. The validity of the proposed model was examined by comparing the linear growth rates measured with the predicted ones obtained from the proposed model. Accordingly, the value of $\frac{K\cdot\mu m}{\alpha\cdot L}log(I_0\cdot{I_s}^{\varepsilon 1)\cdot {I_c}^{-\varepsilon})$ was proposed as an approximate index for strategies to obtain the maximal lightn yield under light-limiting conditions for high-density algal cultures and as a control parameter to improve the photosynthetic productivity and efficiency.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.233-236
• /
• 2000

Bacillus thuringiensis (bt)는 생물학적 살충제 시장에서 가장 널리 사용되는 미생물 살충제이다. Bt의 고농도로 세포배양을 하면 생물반응기에서 원하는 생산물의 농도를 이룰 수 있을 뿐만 아니라 생산물의 생성속도도 이룰 수 있다. 이런 목적 하에서 우리는 세라믹 막 모듈을 반응기 내부에 장착한 새로운 형태의 생물반응기를 개발하였다. 이 새로운 형태의 생물반응기에서 포도당 제한 상태하의 운전을 통해서 bt의 세포와 포자 수율을 현저하게 증가시킬 수 있었다. 최대로 $1.2\;{\times}\;10^{10}\;CFU/ml$의 포자 수율을 달성할 수 있었다. 세라믹 막 모듈 생물반응기에서 포도당 제한 상태 하에서 bt의 성장특성을 연구하였다. 포도당 제한 상태에서는 세포의 성장은 선형 (linear) 성장특성을 보였는데 이것은 간단한 비구조적 수학적 모델의 결과와 잘 일치하였다.

• 한국의류산업학회지
• /
• 제11권5호
• /
• pp.818-826
• /
• 2009

Dyeability of microbial violacein produced from Chromobacterum violaceum CV107 on to multifiber fabrics has been studied. The bluish-purple colourants were produced by cultivation of Chromobacterum violaceum using LB liquid medium for 2 days. The colourant was extracted with 80% acetone and identified as violacein by LC/MS analysis. The violacein could be dyed on not only natural fibers such as Cotton, Silk and Wool but also synthetic fibers such as Diacetate, Triacetate, Creslan 61 and Nylon 66. Maximum K/S values were shown at 540-580 nm according to different fiber with color appearance of purple or blue. An optimum pH and temperature under dyeing condition were 10 and $70^{\circ}C$, respectively. Any mordants were not improved colour density and quality on various fabrics. From this studies, pigments produced microbe have a high potentials for natural dyeing on fabrics. Finally, development of new colourants from microbe has made a possible change for new dyeing field in respects of eco-friend and repeatability of natural dyeing for apparels.

• 한국유기농업학회지
• /
• 제19권spc호
• /
• pp.218-221
• /
• 2011

To improve the soil properties of physical and microbial community rice bran and wood charcoal were applied in the continuously cultivated plastic film house soil. Soil physical properties were improved by application of rice bran and charcoal compared to chemical fertilizer application (control) by 8~14% in bulk density and 5~9% in soil porosity. Changes in the biological ratio indexes of fatty acids in the soils were detected depending on the inputted materials. Especially in application of rice bran including mixture with charcoal, much more fungi and less bacteria were detected and the ratio of fungi to bacteria was increased, suggesting the more organic carbon metabolically active in these treatments. The high ratio of aerobe to anaerobe suggested the better aerobic conditions were in the soil inputted wood charcoal. From these results, it is important and possible to select some materials for the organic pepper cultivation, which may improve the poor condition soil.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2022년도 추계학술대회
• /
• pp.115-115
• /
• 2022

The Food and Agriculture Organization (FAO) has been estimating the potential of insects as human food and animal feed for convincing food security since 2010. On account of this reason, Tenebrio molitor larvae have been gaining considerable attention as an alternative protein source for various foods. However, consumers do not prefer them because of their disgusting appearance. Therefore, it is necessary to develop a method that improve their appearance as to lead to T. molitor larvae consumption. In this study, T. molitor larvae which is prepared for optimally sampling conditions (shade drying, 30 min boiling after hot air drying, 30 min steaming after hot air drying), were cultured with Cordyceps militaris mycelia, and measured growth and density of mycelial. Also analyzed the cordycepin and adenosine content compared with commercial C. militaris. As a result, T. molitor larvae cultivated with C. militaris mycelia showed the highest cordycepin content (13.75 mg/g) was observed in optimal sampling conditions (shade drying). Therefore, we report that the methods which T. molitor larvae cultivated with C. militaris mycelia not only improve their appearance but also increase cordycepin content, which can be contributed to lead T. molitor larvae consumption.

• 한국유기농업학회지
• /
• 제21권3호
• /
• pp.363-372
• /
• 2013

가지 담배가루이 친환경 방제기술을 개발하고자, 난황유 등 8종의 천연물질을 가지 정식 후 7일 간격으로 살포하면서 생육을 조사한 결과 님제처리에서 가장 양호한 경향이었다. 담배가루이 성충과 약충의 발생은 디노테퓨란약제처리에서 가장 적었고, 친환경자재 중에서는 님제 처리에서 가장 적었다. 수량은 디노테퓨란 약제처리에서 가장 많았고, 친환경 자재에서는 님제, 고추씨유 등이 많았으며, 고삼제 은행과육 등에서 적었다. 님제 처리의 경우 무처리에 비하여 69% 많았고, 디노테퓨란약제에 비해 52% 낮은 수량을 나타내었다.

• Journal of Plant Biotechnology
• /
• 제49권1호
• /
• pp.61-73
• /
• 2022

In this study, we developed a reliable and efficient Agrobacterium-mediated genetic transformation system by applying sonication and vacuum infiltration to six chickpea cultivars (ICCV2, ICCV10, ICCV92944, ICCV37, JAKI9218, and JG11) using embryo axis explants. Wounded explants were precultured for 3 days in shoot induction medium (SIM) before sonication and vacuum infiltration with an Agrobacterium suspension and co-cultivated for 3 days in co-cultivation medium containing 100 µM/l of acetosyringone and 200 mg/l of L-cysteine. Responsive explants with putatively transformed shoots were selected using a gradual increase in kanamycin from 25 mg/l to 100 mg/l in selection medium to eliminate escapes. Results showed optimal transformation efficiency at a bacterial density of 1.0, an optical density at 600 nm wavelength (OD₆₀₀), and an infection duration of 30 min. The presence and stable integration of the β-glucuronidase (gusA) gene into the chickpea genome were confirmed using GUS histochemical assay and polymerase chain reaction. A high transformation efficiency was achieved among the different factors tested using embryo axis explants of cv. JAKI 9218. Of the six chickpea cultivars tested, JAKI9218 showed the highest transformation efficiency of 8.6%, followed by JG11 (7.2%), ICCV92944 (6.8%), ICCV37 (5.4%), ICCV2 (4.8%), and ICCV10 (4.6%). These findings showed that the Agrobacterium-mediated genetic transformation system will help transfer novel candidate genes into chickpea.

• 한국작물학회지
• /
• 제59권3호
• /
• pp.375-384
• /
• 2014

양질의 사료작물 소재로서 국내 재배기술에 대한 재배기준이 설정되어 있지 않은 케나프를 2012~2013년 2년 동안 시험처리를 통하여 파종법을 구명한 결과 케나프의 파종방법은 기계화 파종(옥수수 파종기 사용)이 가능한 점파가 수량 증가에 적합하였는데 파종거리를 $20{\times}20cm$로 할 경우 관행 $20{\times}30cm$ 처리에 비해 수량이 32% 증가한 13,641 kg/10a가 가능하였다. 케나프의 최대수량 확보를 위한 적정 파종시기는 5월 1일 이었는데 5월 1일 파종의 경우 생체수량 13,423 kg/10a로 5월 30일 파종 대비 30%가 증수되었고 조단백질 함량도 12.7%로 5월 30일 대비 16.5% 증가하였다. 제초제는 Fluazipof-p-butyl 사용이 가장 효율적이었는데 제초율이 97% 이었고 약해는 5% 이하로 안정적이었다. 케나프의 전북지역 적응성을 임실, 고창, 순창 등 3개 지역에서 확인한 결과 모두 안정적으로 재배가 가능함이 확인되었는데 3개 지역 평균 수량이 12,400 kg/10a로 옥수수에 비해 약 1.7 배 정도 수확하는 것이 가능하였다.

• 한국작물학회지
• /
• 제54권1호
• /
• pp.104-118
• /
• 2009

This study was carried out to classify Korean soybean varieties base on maturity group (MG) and to find character relationships according to planting date for high quality soybean seed production adapted to early season cultivation environment of Miryang. Results of maturity grouping of Korean soybean varieties showed that Keunol (3 cultivars), belonged to Group 0; Seonnok and Danmi in Group II, Shinrok in Group III, Seonyu (17 cultivars), in Group IV, Taekwang (44 cultivars) in Group V, Daewon (25 cultivars) in Group VI, and Kwangdu and Keumdu in Group VII. Agronomic characteristics of 100 soybean varieties were compared based on MG, cultivation year and seeding date. Soybean varieties belonging to the MG $VI{\sim}VII$ showed longer days to flowering and growth period, high lodging density and higher yield. Seed quality analysis revealed that as maturity was delayed, seed weight becomes heavier while seed cracks become abundant. In addition, occurrence of purple seed and phomopsis were higher in MG $0{\sim}III$. Protein content was higher in MG $0{\sim}III$, and isoflavone content was higher as maturity was delayed. On the other hand, lipid content was generally similar across MGs. Correlation analysis of major agronomic characters showed positive relationships between days to flowering and growth days, seed weight and lodging in MG $IV{\sim}V$, seed crack and growth days in MG $0{\sim}III$, seed crack and days to flowering in MG $IV{\sim}V$ and MG $VI{\sim}VII$, seed crack and lodging in MG $IV{\sim}V$ and MG $VI{\sim}VII$, seed crack and seed weight in MG $IV{\sim}V$ and MG $VI{\sim}VII$, purple seed and growth days in MG $IV{\sim}V$, purple seed and seed weight in MG $VI{\sim}VII$, phomopsis and growth days in MG $IV{\sim}V$ and MG $VI{\sim}VII$, and phomopsis and purple seed in MG $IV{\sim}V$ and MG $VI{\sim}VII$. In contrast, a negative relationship was observed between seed weight and lodging in MG $0{\sim}III$. Correlating yield and major characters revealed negative relationships between days to flowering and growth days in MG $0{\sim}III$ and MG $IV{\sim}V$, whereas positive relationships were obtained on MG $VI{\sim}VII$ seeded on April 30. Lodging, seed weight and seed crack were all negatively correlated with yield in the MG $IV{\sim}V$ and MG $VI{\sim}VII$. Soybean cultivars identified as adaptable to early season planting for production of high quality soy curd and fermented soybean paste were Seonyu, Kwangdu, and Soho while those suited for the manufacture of soybean sprouts were Sobaeknanul, Kwangan, Sowon, and Bosuk. Geomjeong 2 chosen as best for mixing with rice.