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Stability and Isolation of Monacolin K from Red Yeast Rice (홍국 유래 Monacolin K의 안정성 및 분리)

  • 최무영;곽은정;임성일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.6
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    • pp.1022-1027
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    • 2004
  • The monacolin K content was determined to investigate the stability of monacolin K from red yeast rice after heating (20∼8$0^{\circ}C$), adjusting the pH (2∼8) by adding 3 N HCl or 3 N NaOH, adding the organic acid (6.0% acetic acid, 0.6% citric acid, 1.5% lactic acid) to pH 4.0 and adding the water (0∼80%). And the monacolin K was isolated from red yeast rice by conducting open column chromatography using neutral aluminum oxide. As a result, the stability of monacolin K decreased with increasing the temperature. The stability for pH was in the order of the unadjusted pH (pH 5.9)>8>4 and pH 2>10. The stability for organic acid was high in the order of lactic acid>citric acid>acetic acid, and the stability of monacolin K under acid was different according to the acid type. The degradation rate of monacolin K increased with increasing the water content. Moreover monacolin K was able to isolate from red and pink pigments as well as the other noncoloric compounds in red yeast rice. The yield of monacolin K was found to be 70%.

Analysis of Salinity Impacts on Agricultural and Urban Water Users

  • Michelsen, Ari;Sheng, Zhuping;McGuckin, Thomas;Creel, Bobby;Lacewell, Ron
    • Proceedings of the Korea Water Resources Association Conference
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    • 2011.05a
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    • pp.13-13
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    • 2011
  • The Rio Grande Compact Commission, in collaboration with local water management entities, water users and universities established a three state Rio Grande The Rio Grande Compact Commission, in collaboration with local water management entities, water users and universities established a three state Rio Grande Salinity Management Program. The objectives of the Rio Grande Project Salinity Management Program are to reduce salinity concentrations, loading, and salinity impacts in the Rio Grande basin for the 270 mile river reach from San Acacia, New Mexico to Fort Quitman, Texasto increase usable water supplies for agricultural, urban, and environmental purposes. The focus of this first phase of the program is the development of baseline salinity and hydrologic information and a preliminary assessment of the economic impacts of salinity. An assessment of the economic impacts of salinity in this region was conducted by scientists at Texas A&M University's AgriLife Research Center at El Paso and New Mexico State University. Economic damages attributable to high salinity of Rio Grandewater were estimated for residential, agricultural, municipal, and industrial uses. The major impact issues addressed were: who is being affected the types of economic impacts the magnitude of economic damages overall and by user category and identification of threshold-effect levels for different types of water use. Salinity concentrations in this 270 mile reach of the river typically range from 480 ppm to 1,200 ppm, but can exceed 3,000 ppm in the lower section of this reach. Economic impacts include reductions in agricultural yields, reduced water appliance life, equipment replacement costs, and increased water supply costs. This preliminary economic assessment indicates annual damages of $10.5 million from increased water salinity. Under current water uses, municipal and industrial uses account for 75% of the total estimated impacts. However, agricultural impacts are based on current crop pattern yield reductions and, salinity leaching requirements and do not account for the impacts of reduced revenue from having to grow salinity tolerant, lower value crops. Actual damages are anticipated to be significantly higher with the inclusion of these additional agricultural impacts plus the future impacts from the growing population in the region. A more comprehensive economic analysis is planned for the second phase of this program. Results of the economic analysis are being used to determine the feasiblity of salinity control alternatives and what salinity reduction control measures will be pursued.

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Improvement of Immune Activities of Rhodiola sachalinensis A. Bor. by Serial Solvent Fractionization (홍경천의 면역활성이 증진된 용매별 분획 추출물)

  • Ha, Ji-Hye;Kim, Cheol-Hee;Jeong, Hyang-Suk;Jin, Ling;Oh, Sung-Ho;Kim, Seung-Seop;Jeong, Myoung-Hoon;Ma, Choong-Je;Nam, Jong-Hyun;Hwang, Baik;Choi, Geun-Pyo;Park, Uk-Yeon;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.3
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    • pp.210-216
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    • 2009
  • This study was performed to compare the effects of immuno-modulating activities of Rhodiola sachalinensis A. Bor. fractionized by consecutive solvent separation. The Cytotoxicity of all fractionized extracts on human kidney cell (HEK293) was lower than crude extracts. Generally, the butanol and chloroform extracts showed less cytotoxicity on about 10.07% and 9.67% than the crude extracts. For human immune B and T cell growth, chloroform fraction showed the highest cell growth compared to the control. The secretion of cytokines (IL-6, $TNF-\alpha$) on human B and T cells were increased by adding chloroform extracts. Also, NK cell growth was significantly improved up to nearly 30% by adding the supernatant of B cell medium grown with the chloroform fraction. It was also found that chloroform fraction could yield higher nitric oxide production from macrophage than untreated control cells. Differentiation of HL-60 cells was increased up to 131.9% after treatment with chloroform fraction extracts, compared to the control. These results indicate that the chloroform fraction of R. sachalinensis have high immune activation activity than others fractions and the crude extracts, implying that this chloroform fractions could be used a new functional material.

A New Early Maturity, High Grain Quality and Cold Tolerance Rice Cultivar "Boseog" (벼 조생 고품질 내냉성 "보석")

  • Nam, Jeong Kwon;Kim, Ki Young;Choung, Jin Il;Shin, Mun Sik;Kim, Bo Kyeong;Ha, Ki Yong;Ko, Jong Cheol;Kang, Hyeon Jung;Shin, Woon Chul;Mo, Young Jun;Ko, Jae Kwon
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.564-567
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    • 2009
  • "Boseog" is a new japonica rice cultivar developed from the three way cross of Kinuhikari//HR19621AC6/Sobibyeo and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2008. This cultivar has about 110 days of growth duration from transplanting to harvesting in northern plain, northern and southern mid-mountainous area of Korea. This cultivar is about 72 cm in culm length and tolerant to lodging. In the reaction to biotic and abiotic stresses, it shows resistance to leaf blast, but susceptibility to bacterial blight, virus diseases and insect pests. The milled rice of "Boseog" exhibits translucent, relatively clear non-glutinous endosperm and midium short grain. It has similar amylose content(18.0%), lower protein content(6.3%), and similar palatability of cooked rice compared to Odaebyeo. The milled rice yield of this variety is about 5.03 MT/ha in local adaptability test. "Boseog" would be adaptable for ordinary transplanting in northern plain, mid-mountainous and southern mountainous area of Korea.

Breeding of New, Orange Mini/Medium Type, High Yielding Cut Flower Gerbera hybrida 'Sweety' (오렌지색 중.소륜 절화용 다수성 거베라 신품종 '스위티' 육성)

  • Chung, Yong Mo;Hwang, Ju Chean;Chin, Young Don;Kim, Su Kyung;Kwon, Oh Chang
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.2
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    • pp.124-127
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    • 2008
  • A new orange-colored, cut flower gerbera (Gerbera hybrida Hort.) cultivar 'Sweety' was developed from a cross between 'Grandeur' and 'Songsongee', followed by selections of seedlings and lines at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 1999 to 2004. Three time evaluations were conducted from 2003 for the detailed characteristics of the new cultivar. 'Sweety' has semi-double type mini- or medium-sized flowers in good harmony with orange(RHS, 38-A) ray florets and a green center. It has good, stable flower shape and strong peduncle, and its vase life was 10.4 days. The average flower yield was about 51.0 stems per plant/year in greenhouse trails during 2003 and 2004. This cultivar was registered to the Korea Seed and Variety Service for commercialization in 2006. Year-round production of this cultivar is possible in the greenhouse condition in Korean climate.

A Study on the Repair of Fatigue Damage at Large Cast Iron structure using Cold Joint Method (냉간체결방법을 이용한 대형 주철 구조물의 피로손상수리방법에 대한 연구)

  • Lee, Sung-Riong;Lee, Dong Jun;Cho, Seok Swoo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.10
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    • pp.140-148
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    • 2019
  • Large cast iron structures are used in casings and pipes in shipsand chemical plants. Broken parts in the casings and pipescan result in failures even when stresses are below the yield strength of the part's materials. Fatigue failure of a large cast iron structure is inevitable due to the design constraints and low reliability of the material strength. A small structure can be repaired by welding, but a large structure cannot because it cannot be preheated slowly and uniformly. This study shows that a large structure can be repaired by a cold joint method using a crack repair screw. Large cast iron structures were manufactured by GC 300, and their design stress is below 3.5 MPa. The tensile strength on notched specimens repaired by crack repair screws was 8.2 MPa. Therefore, the safety factors of structures repaired by crack repair screws have a value above 2.3 and are considered to be high values.

Preparation and Characterization of Nylon 6,5 Copolymers from ε-Caprolactam and 2-Piperidone (ε-Caprolactam과 2-Piperidone으로부터 나일론 6,5 공중합체 제조 및 특성평가)

  • Kim, Hye Young;Goh, Jin-Seon;Ryu, Mi Hee;Kim, Dae Su;Song, Bong-Keun;Lee, Seung Hwan;Park, Si-Jae;Jegal, Jonggeon
    • Polymer(Korea)
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    • v.38 no.1
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    • pp.31-37
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    • 2014
  • To prepare biomass based nylon 6,5 copolymers, ${\varepsilon}$-caprolactam and 2-piperidone, the monomers of nylon 6,5 copolymers, were synthesized respectively from lysine and 5-aminovaleric acid which were produced from glucose by the fermentation process. The copolymers were then polymerized by the anionic ring opening polymerization of them at $40^{\circ}C$, using potassium tert-butoxide as a catalyst and acetyl-2-caprolactam and carbon dioxide as initiators. The prepared copolymers were characterized with various analytical methods: their viscosity molecular weight ($M_{\eta}$) was as high as 30000 g/mol and polymerization yield was over 50%, and it was found that they were semi-crystalline polymers having melting point at $165^{\circ}C$ which was much lower than its thermal degradation point, $250^{\circ}C$. These polymers were expected to have good thermal processability and application fields.

SHRIMP U-Pb Zircon Geochronology of the Guryong Group in Odesan Area, East Gyeonggi Massif, Korea: A new identification of Late Paleozoic Strata and Its Tectonic Implication (경기육괴 동부 오대산 지역의 구룡층군에 대한 SHRIMP U-Pb 저어콘 연대측정: 새로운 후기 고생대층의 인지와 지체구조적 의의)

  • Cho, Deung-Lyong
    • The Journal of the Petrological Society of Korea
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    • v.23 no.3
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    • pp.197-208
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    • 2014
  • Zircon separated from a biotite schist of the Guryong Group in Odesan area, eastern part of the Gyeonggi Massif in Korea were analysed for SHRIMP U-Pb ages. CL images display composite core-rim structures of the zircon, indicating an in-situ overgrowth of zircon through a high-grade metamorphism. The metamorphic zircon rims give a weighted mean age of $247{\pm}6Ma$. While the detrital zircon cores have zoning patterns and Th/U ratios indicative of a magmatic origin. Among 53 analyses from the cores, 46 data yield near concordant ages which are concentrated at $378{\pm}10Ma$ (n=9), $420{\pm}4Ma$ (n=6) and $1845{\pm}9Ma$ (n=18) with sporadic Neoproterozoic ($687{\pm}9Ma$) to late Archean ($2519{\pm}20Ma$) ages. The age data constraint sedimentation age of protolith of the Guryong Group, so far unknown, as late Paleozoic. The Guryong Group of this study is the first late Paleozoic strata reported from eastern Gyeonggi Massif, and its maximum depositional age (ca 378 Ma) is identical with those of the late Paleozoic strata in the southwestern Ogcheon Belt. The Triassic metamorphic age and abundant middle Paleozoic provenance (361~425 Ma) of the Guryong Group are similar with those reported from the Triassic collisional belt in central China. Thus this study indicates that the Odesan area would be an possible eastward extension of the Triassic collisional belt in central China.

Isolation and Identification of Antimicrobial Active Substances from Rhodiola sachlinensis (홍경천(Rhodiola sachlinensis)에서 항균성 물질의 분리 및 동정)

  • 심창주;이규희;정재홍;이상덕;김영호;오만진
    • Food Science and Preservation
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    • v.11 no.1
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    • pp.63-70
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    • 2004
  • The antimicrobial substances from Rhodiola sachalinensis were extncted, isolated and identified. The highest level of antimicrobial activity and its yield were obtained in methanol extract. The minimum inhibition concentrations of the methanol extract were 500 $\mu\textrm{g}$mL on agar plate and 100 $\mu\textrm{g}$mL in broth media for four gram positive and four gram negative microbials. The methanol extract was fractionated by n-hexane, chloroform, ethyl ether, ethyl acetate, and butanol, orderly. The separate was developed on the TLC plate with different solvent system ratio of chloroform and methanol. Nine substances were isolated from chloroform and methanol mixture(9:1, v/v). Among them, three isolates showed antimicrobial activity. Three substances separated by HPLC were identified by GC/MS(EI) spectrum and $^1$H, /sup13/C-NMR spectrum. They were gallic acid, (-)-epicatechin and kaempferol. The antimicrobial activities of each substances were shown gallic acid, (-)-epicatechin, kaempferol orderly.

Effects of Temperature and Photoperiod on the Growth of Tatary Buckwheat(Fagopyrum tataricum) (온도 및 일장처리가 쓴 메밀(Fagopyrum tataricum)의 생육에 미치는 영향)

  • 박병재;장광진;박종인;박철호
    • Korean Journal of Plant Resources
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    • v.17 no.3
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    • pp.352-357
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    • 2004
  • This study was carried out to investigate the effects of temperature and photoperiod on the growth of Fagopyrum tataricum. It showed a tendency to promote the germination rate and plant height of tartary buckwheat under high temperature. Plant height and number of leaves were promoted in the order of 25 > 20 > 15 > 3$0^{\circ}C$ under the different temperature after 30 days of the planting. In the 10, 12, 14hr photoperiods at 2$0^{\circ}C$, plant height, number of leaves and dry weight were increased as much as photoperiod became long except by 16hr. Rutin contents was not regular both common and tartary buckwheat under the photoperiods. The rutin content in leaves was higher than stem. Compared to yield of tartary and common buckwheat, plant height, number of leaves, number of branch and stem diameter of tartary buckwheat increased more than common buckwheat. Especially, number of leaves was increased about 2.5 times more than common buckwheat. And there was a difference in about 2.1 times in grain weight and about 5.4 times in number of grains. Rutin content was 1469.8mg/l00g in grain of tartary buckwheat. It was about 60 times higher than 22mg/100g in grain of common buckwheat.