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Improvement of Immune Activities of Rhodiola sachalinensis A. Bor. by Serial Solvent Fractionization  

Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National Univ.)
Kim, Cheol-Hee (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jeong, Hyang-Suk (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jin, Ling (College of Bioscience and Biotechnology, Kangwon National Univ.)
Oh, Sung-Ho (College of Bioscience and Biotechnology, Kangwon National Univ.)
Kim, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National Univ.)
Ma, Choong-Je (College of Bioscience and Biotechnology, Kangwon National Univ.)
Nam, Jong-Hyun (Grami Co., Ltd.)
Hwang, Baik (Department of Biology, Chonnam National Univ.)
Choi, Geun-Pyo (Department of Food Processing & Bakery, Gangwon Provincial Univ.)
Park, Uk-Yeon (Department of Food Processing & Bakery, Gangwon Provincial Univ.)
Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.3, 2009 , pp. 210-216 More about this Journal
Abstract
This study was performed to compare the effects of immuno-modulating activities of Rhodiola sachalinensis A. Bor. fractionized by consecutive solvent separation. The Cytotoxicity of all fractionized extracts on human kidney cell (HEK293) was lower than crude extracts. Generally, the butanol and chloroform extracts showed less cytotoxicity on about 10.07% and 9.67% than the crude extracts. For human immune B and T cell growth, chloroform fraction showed the highest cell growth compared to the control. The secretion of cytokines (IL-6, $TNF-\alpha$) on human B and T cells were increased by adding chloroform extracts. Also, NK cell growth was significantly improved up to nearly 30% by adding the supernatant of B cell medium grown with the chloroform fraction. It was also found that chloroform fraction could yield higher nitric oxide production from macrophage than untreated control cells. Differentiation of HL-60 cells was increased up to 131.9% after treatment with chloroform fraction extracts, compared to the control. These results indicate that the chloroform fraction of R. sachalinensis have high immune activation activity than others fractions and the crude extracts, implying that this chloroform fractions could be used a new functional material.
Keywords
Rhodiola sachalinensis A. Bor.; Consecutive Solvent Fraction; Immune Activation Activity; Natural Killer (NK) Cell;
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Times Cited By KSCI : 5  (Citation Analysis)
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