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Improvement of Immune Activities of Rhodiola sachalinensis A. Bor. by Serial Solvent Fractionization  

Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National Univ.)
Kim, Cheol-Hee (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jeong, Hyang-Suk (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jin, Ling (College of Bioscience and Biotechnology, Kangwon National Univ.)
Oh, Sung-Ho (College of Bioscience and Biotechnology, Kangwon National Univ.)
Kim, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National Univ.)
Ma, Choong-Je (College of Bioscience and Biotechnology, Kangwon National Univ.)
Nam, Jong-Hyun (Grami Co., Ltd.)
Hwang, Baik (Department of Biology, Chonnam National Univ.)
Choi, Geun-Pyo (Department of Food Processing & Bakery, Gangwon Provincial Univ.)
Park, Uk-Yeon (Department of Food Processing & Bakery, Gangwon Provincial Univ.)
Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.3, 2009 , pp. 210-216 More about this Journal
Abstract
This study was performed to compare the effects of immuno-modulating activities of Rhodiola sachalinensis A. Bor. fractionized by consecutive solvent separation. The Cytotoxicity of all fractionized extracts on human kidney cell (HEK293) was lower than crude extracts. Generally, the butanol and chloroform extracts showed less cytotoxicity on about 10.07% and 9.67% than the crude extracts. For human immune B and T cell growth, chloroform fraction showed the highest cell growth compared to the control. The secretion of cytokines (IL-6, $TNF-\alpha$) on human B and T cells were increased by adding chloroform extracts. Also, NK cell growth was significantly improved up to nearly 30% by adding the supernatant of B cell medium grown with the chloroform fraction. It was also found that chloroform fraction could yield higher nitric oxide production from macrophage than untreated control cells. Differentiation of HL-60 cells was increased up to 131.9% after treatment with chloroform fraction extracts, compared to the control. These results indicate that the chloroform fraction of R. sachalinensis have high immune activation activity than others fractions and the crude extracts, implying that this chloroform fractions could be used a new functional material.
Keywords
Rhodiola sachalinensis A. Bor.; Consecutive Solvent Fraction; Immune Activation Activity; Natural Killer (NK) Cell;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Abo T, Kawamura T and Watanabe H (2005), Immunologic states of autoimmnu diseases, Immunologic Research, 33:23-34   DOI   PUBMED   ScienceOn
2 Cui CB, Lee DS and Ham SS. (2003), Antioxidative, actimutagenic and cytotoxic effects of Rhodiola sachalinensis extracts, Journal of Korean Society of Food Science and Nutrition, 32:211-216   DOI
3 Doll R and Peto R. (1983), The cause of cancer : quantitative estimates of avoidable risks of cancer in the United States todays, Journal of the National Cancer Institute, 66:191-308
4 Edmund KW and Wolf PS. (1985), Induction of fibrinolytic activity in Hela cells by phorbol myristate acetate, Korean Society for Applied Biological Chemistry, 260:6354-6360
5 Ha JH, Han JG, Jeong HS, Oh SH, Kwon MC, Choi YB, Ko JR and Lee HY. (2008), Enhancement of immune activities of natural water-soluble sulforaphane by nano encapsulation process, Korean Journal of Medicinal Crop Science, 16:402-408
6 Han BH, Park MH, Cho JY, Park JS, Yoo ES and Bail KU(1998), Effect of ginsenosides from Panax ginseng on TNF-$\alpha$ production and T cell proliferation, Yakhak Hoeji, 42:296-301   과학기술학회마을
7 Kim CH, Kim HS, Kwon MC, Bae GJ, Ahn JH, Lee HJ, Kang HY and Lee HY, (2006b), Increase effect of anticancer activities on Rhodiola sachalinensis A. Bor, by the change of extraction process, Korean Journal of Medicinal Crop Science, 14:317-321   과학기술학회마을
8 Yueran Z, Rui S, You L, Chunyi G and Zhigang T. (2003), Expression of leptin receptors and response to leptin stimulation of human natural killer cell lines, Biochemical and Biophysical Research Communications, 300:247-252   DOI   ScienceOn
9 Yen Y and Cuernsey DL (1986), Increased c-myc RNA levels associated with the precommitment state during HL-60 myeloid differentiation, Cancer Research 46:4156-4161   PUBMED
10 Lishmanov YB, Maineskulova LA, Uskina EV and Maslov LN(1999), Opiatergic mechanisms of cardioprotective and antiarrhythmic effects of adaptation, Bulletin of Experimental Biology and Medicine, 127:151-154   DOI
11 Kwon MC, Han JG, Qadir SA, Ahn JH, Lee DH and Lee HY(2008), Enhancement of immuno-poteniation of Cichorium endivia L, by ultrasonification extraction process, Korean Journal of Medicinal Crop Science, 16:9-15
12 Kim JY, Seong NS and Lee YJ. (2006a). A study on the effects of Rhodiola rosea root on the cancers. Korean Journal of Herbology. 21:79-87
13 Ding AH, Nathan CF and Stuhr DJ. (1998), Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages, Journal of Immunology,141:2407-2412
14 Ryu SH, Kim SY, Jung HS, Sohn NW and Sohn YJ. (2008), Effects of Rhodiola Rosea on anti-fatigue and hypothalarnic IEGs expressions of forced swimming rats, Korea Journal of Herbology, 23:9-19
15 Cecilia KL. (2002), IL-2 receptor signaling through the shbadapter protein in T and NK cells, Biochemical and Biophysical Research Communications, 296:929-936   DOI   PUBMED   ScienceOn
16 Park HU, Yoon JH, Kim JY, Jeong CH, Park CK, Song WS and Seo KI (2005), Biological activity of the fractions extracted from Rhodiola dumulosa, Korean Society of Food Preservation 12:596-500
17 Kim CH, Kwon MC, Han JG, Ha JH, Jeong HS, Choi GP, Park UY, Nam JH, Hwang B and Lee HY, (2008), Immune activities of Rhodiola sachalinensis A. Bor. extracts isolated with various extraction process, Korean Journal of Medicinal Crop Science, 16:383-389
18 Bang OS, Lee YS and Kang SS. (1993), Apossible role of fibronectin on the differentiation of monocyte to macrophage, Korean Journal of Zoology, 36:514-521
19 Kwon MC, Han JG, Jeong HS, Qadir SA, Choi YB, Ko JR and Lee HY. (2008), Enhancement of immune activities of opuntia ficus-india L, var. Miller by ultrasonification extraction process, Korean Journal of Medicinal Crop Science, 16:1-8
20 Afanas' ev SA, Krylatov AV, Lasukosa TV and Lishmanov YB(1996), Participation of inducible stress proteins in the cardioprotective effects of Rhodiolae rosea, Biocheminstry(Moscow), 61:1258-1263