• 한국정밀공학회지
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• 제31권11호
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• pp.1031-1040
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• 2014

In this paper, a new condition diagnosis algorithm for the lens injection molding process using various features extracted from cavity pressure, nozzle pressure and screw position signals is developed with the aid of probability neural network (PNN) method. A new feature extraction method is developed for identifying five (5), seven (7) and two (2) critical features from cavity pressure, nozzle pressure and screw position signals, respectively. The node energies extracted from cavity and nozzle pressure signals are also considered based on wavelet packet decomposition (WPD). The PNN method is introduced to build the condition diagnosis model by considering the extracted features and node energies. A series of the lens injection molding experiments are conducted to validate the model, and it is demonstrated that the proposed condition diagnosis model is useful with high diagnosis accuracy.

• 한국식품과학회지
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• 제41권3호
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• pp.284-291
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• 2009

본 연구에서는 저온고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 저온고압 추출공정에 의한 매자나무 수피의 항산화활성과 항암활성의 증진을 확인하고자 연구를 수행 하였다. DPPH radical 소거 활성은 5분 저온고압 처리한 추출물이 93%로 가장 높은 활성을 나타내며 대조군인 ascorbic acid의 95%와 유사한 활성을 나타내었다. 저온고압 추출공정을 통한 매자나무 추출물이 일반 열수 추출물과 비교해 xanthine oxidase 저해활성이 높게 나타났으며 특히 5분 저온고압 처리한 추출물이 56.68%의 높은 저해 활성을 나타냈다. 따라서 저온고압 추출공정을 통해 매자나무 추출물의 항산화 활성의 증진이 이루어졌음을 알수 있다. 항암활성 측정 결과, 매자나무 수피 추출물의 높은 항암활성을 확인하였으며 5분 저온고압 처리한 추출물의 암세포 억제 활성이 가장 높게 나타남에 따라 저온고압 추출공정을 통해 항암활성의 증진이 가능함을 확인하였다. 세포독성 측정 결과 5분 저온고압 추출물이 정상 세포에 대한 독성이 가장 높은데 반해 15분 저온고압 처리한 추출물의 세포독성이 제일 낮은 것으로 미루어 저온고압 추출물의 세포독성이 저온고압 추출시간의 영향을 받는 것으로 사료된다. 따라서 매자나무 수피의 생체적용이 가능한 기능성 소재로서의 이용 가능성을 확인할 수 있으며 저온 고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 높은 경제적 가치를 부여해 줄 수 있을 것으로 사료된다.

• 한국약용작물학회지
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• 제21권1호
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• pp.54-60
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• 2013

According to previous reports, antioxidant activities of Codonopsis lanceolata could be increased by a steaming process. This study was performed to improve its antioxidant activity and skin whitening activities of C. lanceolata by high pressure and stepwise steaming complex process. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%, and for phenol and flavonoid contents, complex processed C. lanceolata contained higher than those from conventional extraction process or steaming process alone. The Cytotoxicity of all C. lanceolata extracts also showed low cytotoxicity against human fibroblast cell (CCD-986sk) as 4.49 ~ 10.40%. In whitening activity, high inhibition of tyrosinase activity was estimated as 25.08% by adding the extracts from complex process. We found that whitening and antioxidant activity of complex processed C. lancolata extract was higher than those obtained from conventional extraction and a steaming process because various kinds of antioxidant compounds could be easily released by combined process, compared to one of each process.

• Bulletin of the Korean Chemical Society
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• 제19권12호
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• pp.1351-1355
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• 1998

A simple apparatus for adding a modifying solvent to supercritical CO2 extractant was described. Small, fixed volumes (typically 100 μL) of liquid modifying solvents were delivered during the extraction process by use of an in-line high pressure loop injector and an air pump. Without disconnecting the extraction cell from the supercritical fluid extraction system, the modifying solvent was repeatedly delivered. The solvent modification device was optimized during the extraction of carbaryl and bis(acetylacetonato) copper(Ⅱ). Extraction recoveries from spiked filter paper and soil samples ranged between 22% and 109%, depending on the analyte and matrix components. The addition of polar modifying solvents were necessary to improve the extractability of the nonpolar CO2.

• 한국약용작물학회지
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• 제18권4호
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• pp.248-254
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• 2010

The objectives of this study were to compare the antioxidant activities by high pressure extraction of Codonopsis lanceolata from different cultivation areas; Hoeng-sung, Jeju island, and China. Total phenolic acid contents of Hoeng-sung, Jeju, China were estimated as 732.11, 640.25, and 584.85 mg QUR/100 g DW, respectively. The flavonoids contents of Hoeng-sung, Jeju, China were measured as 80.37, 76.46, and 74.55 mg QUR/100g DW, respectively. Generally, contents of phenolic acid and flavonoids, HPE was higher than conventional extraction process. Hoeng-sung Codonopsis lanceolata showed 64.33% of DPPH radical scavenging activity (EDA, %) in 3.2 mg/$m{\ell}$ of Hoeng-sung Codonopsis lanceolata. The reducing power of Hoeng-sung cultivation area Codonopsis lanceolata also showed the high activity as 3.15. In generally, antioxidant activities of Codonopsis lanceolata were increased by high pressure extraction process. Based on these results, higher contents of flavonoids and total polyphenols were found extracted by high pressure extraction of Codonopsis lanceolata grow in Hoeng-sung area than others.

• 한국약용작물학회지
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• 제17권6호
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• pp.397-406
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• 2009

The low quality fresh ginseng was extracted by water at $80^{\circ}C$ and 240 bar for 20 min (HPE, High pressure extraction process). The cytotoxicity on human normal kidney cell (HEK293) and human normal lung cell (HEL299) of the extracts from HPE showed 28.43% and 21.78% lower than that from conventional water extraction at $100^{\circ}C$ in adding the maximum concentration of $1.0\;mg/m{\ell}$. The human breast carcinoma cell and lung adenocarcinoma cell growth were inhibited up to about 86%, in adding $1.0\;mg/m{\ell}$ of extracts from HPE. This values were 9-12% higher than those from conventional water extraction. On in vivo experiment using ICR mice, the variation of body weight of mice group treated fresh ginseng extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The extracts from HPE was showed longer survival times as 35.65% than that of the control group, and showed the highest tumor inhibition activities compared with other group, which were 70.64% on Sarcoma-180 solid tumor cells. On the high performance liquid chromatogram (HPLC), amount of ginsenoside-$Rg_2$, $Rg_3$, $Rh_1$ and $Rh_2$ on fresh ginseng were increased up to 43-183% by HPE, compared with conventional water extracts. These data indicate that HPE definitely plays an important role in effectively extracting ginsenoside, which could result in improving anticancer activities. It can be concluded that low quality fresh ginseng associated with this process has more biologically compound and better anticancer activities than that from normal extraction process.

• Natural Product Sciences
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• 제28권2호
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• pp.89-92
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• 2022

In Asia, the roots of Paeonia lactiflora and Astragalus membranaceus have been used as therapeutic agents for thousands of years. Once the medicinal plants are harvested, they are dried and their ingredients are extracted by heat-mediated reflux extraction. However, the condensed structure of organic products (especially roots) limits the extraction of bioactive components. In this study, we assessed the effect of the puffing method (using high temperature and pressure) before the extraction process in relation to the profile and antioxidant capacity of active ingredients. We demonstrated that the additional puffing process before extraction methods improves the yield of polyphenol concentrations and antioxidant activities from the roots of P. lactiflora and A. membranaceus.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.282-288
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• 2009

The present paper describes the effects of pressure and post-mortem aging treatments on in situ proteasome activity in rabbit and bovine skeletal muscles. Synthetic peptide hydrolyzing activity of rabbit proteasomes remained in the muscle after exposure to pressures up to 100 MPa. However, when a pressure of 400 MPa or more was applied, proteasomes were markedly inactivated. The extraction of proteasomes from excessively pressurized muscle appeared to be difficult. Proteasomes in aged muscle remained relatively stable throughout the aging process, with activity after 168 h (7 days) being 35%, 48%, 53% and 31% of the 0 h post-mortem LLVY, LSTR, AAF and LLE total hydrolyzing activities, respectively. The synthetic peptide hydrolyzing activities of bovine muscle proteasomes were similar to those of rabbit skeletal muscle proteasomes. The results suggest that synthetic peptide hydrolyzing activity remains in muscle exposed to relatively low pressures. Furthermore, it is known that high-pressure treatment induces fragmentation of myofibrils, modification of actin-myosin interaction and activation of intramuscular proteinases, cathepsins and calpains. Thus, proteasomes are probably involved in the tenderization process in combination with other intramuscular proteinases under high-pressure conditions. Our findings confirmed that proteasomes play a role in meat tenderization induced by high-pressure treatment or aging.

• 한국식품저장유통학회지
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• 제22권5호
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• pp.714-720
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• 2015

본 연구는 산업 폐기물인 비지를 활용하여 부가 가치가 높은 가공식품을 개발하기 위한 일환으로 수행되었다. 즉 대두 가공 부산물인 비지를 활용하여 죽류, 음료류 및 유동식 등의 제품으로 개발하기 위해, 초고압균질 가공 기술을 활용하여 초미세 비지 현탁액을 제조하고 추출수율, 영양성분 및 항산화성을 확인하였다. 비지 분산액을 homogenizer를 이용하여 1,0000 rpm에서 5분 분쇄한 후 1,000bar, 1,500bar 및 2,000bar의 압력을 가해 고압균질 가공 처리하였다. 고압균질 가공처리한 모든 실험구가 대조군에 비하여 추출수율이 유의적으로 증가하였다(p<0.05). 초고압균질 처리 후 총당, 환원당 및 가용성 식이섬유의 함량은 유의적으로 증가하였으며, 불용성 식이섬유는 유의적으로 감소하였다(p<0.05). 단백질 함량과 유리아미노산 함량은 대조군보다 높은 경향을 나타내었다. 총 폴리페놀 함량은 처리 압력이 높아질수록 유의적으로 증가하였으며, ABTS 라디칼 소거능도 유의적으로 증대되었다(p<0.05). 초고압균질 가공 기술은 비지의 영양성분과 항산화성을 증가시키는 매우 효과적인 가공방법이며, 비지의 영양성분을 효율적으로 활용하여 가공식품을 제조할 수 있음을 확인하였다.

• 한국약용작물학회지
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• 제15권6호
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• pp.411-416
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• 2007

본고에서는 초고압 추출 공정을 통한 홍경천의 독성 저감 및 항암활성 효과를 알아보기 위하여 실험을 실시하였는데 그 결과는 아래와 같다. 인간의 정상 신장세포인 HEK293을 이용한 세포 독성은 초고압으로 15분간 처리한 것에서는 1.0 $mg/m{\ell}$의 농도에서 최고 20.4%의 세포독성을 보였으며, 초고압으로 5분간 처리한 것은 22.5%로 초고압 시간이 길어질수록 세포독성이 낮아지는 것을 확인했다. 일반 추출물은 25.3%로 초고압 처리한 군에 비해 높은 독성을 나타내어 초고압 처리 시 독성이 저감되는 것을 확인하였다. 초고압 처리 군은 또한 HEL299를 이용한 세포독성 실험에서도 1.0 $mg/m{\ell}$의 농도에서 일반 추출물인 WE보다 5% 정도 낮은 독성을 나타냈다. 항암활성 측정은 A549, AGS, MCF-7, Hep3B의 암세포를 실험에 사용하여 실시하였다. 모든 암세포에서 1.0 $mg/m{\ell}$의 농도에서 75% 이상의 높은 억제활성을 보였고, 또한 암세포의 생육활성에 대한 정상 세포의 세포독성의 비로 나타낸 선택적 사멸도는 0.6 $mg/m{\ell}$에서 가장 높은 수치를 보였는데 모두 4이상으로 나타났으며, 15분 동안 초고압 추출을 한 것이 가장 높게 나타났다. 본 실험 결과를 통하여 초고압으로 인해 기존의 추출 방법으로는 추출되지 않았던 유용생리활성 물질들이 초고압으로 인해 조직과 세포막 파괴로 인해용출량 증가로 인해 수율이 증가하였으며, 홍경천의 독성 물질이 파괴되는 것으로 판단된다. 단순한 침전 추출법에 의한 추출방법보다는 초고압 추출에 의한 추출로 식물체에 함유되어 있는 성분 추출의 수율을 배가 시킬 수 있으며, 홍경천 자체 성분에 약간의 변화를 가할 수 있을 것으로 생각된다. 홍경천 성분의 변화는 초고압 추출을 통하여 에너지 수준이 제한된 수소결합, 전기적 결합, 반데르발스결합, 수소결합과 같은 약한 결합들이 분리됨으로써 성분의 변화가 있을 것으로 보인다.