Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Enhancement of the Antioxidant and Anticancer Activities of Berberis koreana Bark by Using a Low Temperature and High-Pressure Extraction Process

저온고압 추출공정을 이용한 매자나무 수피의 항산화 및 항암활성 증진

  • Jin, Ling (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Chung, Eul-Kwon (Myung Jin Farm Coperation) ;
  • Chung, Ae-Ran (Myung Jin Farm Coperation) ;
  • Kim, Jin-Chul (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ahn, Ju-Hee (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Hyeon-Yong (Research Institute of Bioscience and Biotechnology, Kangwon National University)
  • 김영 (강원대학교 BT 특성화학부대학) ;
  • 하지혜 (강원대학교 BT 특성화학부대학) ;
  • 정명훈 (강원대학교 BT 특성화학부대학) ;
  • 정을권 (명진농장) ;
  • 정애란 (명진농장) ;
  • 김진철 (강원대학교 BT 특성화학부대학) ;
  • 안주희 (강원대학교 BT 특성화학부대학) ;
  • 이현용 (강원대학교 생명공학연구소)
  • Published : 2009.06.30
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Abstract

This study was conducted to characterize the enhanced antioxidant and anticancer activities of Berberis koreana bark following a low temperature and high pressure extraction process. The results indicate that the B. koreana bark extracted as described showed a 93% increase in DPPH radical scavenging activity. Inhibition activity of xanthine oxidase was highest by this extraction process. In addition the growth of human lung cancer cells (A549), human stomach cancer cells (AGS), human breast cancer cells (MCF-7) and human liver cancer cells (Hep3B) were inhibited by 70.8%, 86.2%, 84.3% and 62.5% respectively. These data indicate that this low temperature and high pressure extraction technique results in the efficient extraction of bioactive compounds from rigid plant materials. This process could also be combined with other techniques to improve extraction yields and identify new biologically active substances from relatively hard plants.

본 연구에서는 저온고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 저온고압 추출공정에 의한 매자나무 수피의 항산화활성과 항암활성의 증진을 확인하고자 연구를 수행 하였다. DPPH radical 소거 활성은 5분 저온고압 처리한 추출물이 93%로 가장 높은 활성을 나타내며 대조군인 ascorbic acid의 95%와 유사한 활성을 나타내었다. 저온고압 추출공정을 통한 매자나무 추출물이 일반 열수 추출물과 비교해 xanthine oxidase 저해활성이 높게 나타났으며 특히 5분 저온고압 처리한 추출물이 56.68%의 높은 저해 활성을 나타냈다. 따라서 저온고압 추출공정을 통해 매자나무 추출물의 항산화 활성의 증진이 이루어졌음을 알수 있다. 항암활성 측정 결과, 매자나무 수피 추출물의 높은 항암활성을 확인하였으며 5분 저온고압 처리한 추출물의 암세포 억제 활성이 가장 높게 나타남에 따라 저온고압 추출공정을 통해 항암활성의 증진이 가능함을 확인하였다. 세포독성 측정 결과 5분 저온고압 추출물이 정상 세포에 대한 독성이 가장 높은데 반해 15분 저온고압 처리한 추출물의 세포독성이 제일 낮은 것으로 미루어 저온고압 추출물의 세포독성이 저온고압 추출시간의 영향을 받는 것으로 사료된다. 따라서 매자나무 수피의 생체적용이 가능한 기능성 소재로서의 이용 가능성을 확인할 수 있으며 저온 고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 높은 경제적 가치를 부여해 줄 수 있을 것으로 사료된다.

Keywords

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