• Title/Summary/Keyword: high cell density

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Stack Performances of Proton Exchange Membrane Fuel Cell

  • Kho, Young-Tai;Cho, Won-Ihl;Park, Yong-Woo-
    • Proceedings of the Korea Society for Energy Engineering kosee Conference
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    • 1994.11a
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    • pp.14-16
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    • 1994
  • The development of proton exchange membrane fuel cells(PEMFCs) with high energy efficiencies and high power densities is gaining momentum because their performance characteristics are attractive for terrestrial(power sources for electrical vehicles, stand-by power), space and underwater application[1]. Fuel cells are capable of running on non-petroleum fuels such as methanol, natural gas or hydrogen and also have major impact on improving air quality. They virtually eliminate particulates, NO$_{x}$, SO$_{x}$, and significant reduce hydrocarbons and carbon monoxide. Especially, fuel cell-battery hybrid power sources appear to be well suited to overcome both the so-called battery problem(low energy density) and the fuel cell problem(low power density)[2].[2].

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Calcium Ion Effect on the Sugar-H+ -Cotransport System in Chlorella vulgaris (Chlorella vulgaris의 당류 능동수송계에 미치는 칼슘 이온의 영향)

  • 조봉희
    • Journal of Plant Biology
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    • v.36 no.4
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    • pp.321-326
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    • 1993
  • Sugar uptake is accompanied with H+-substrate-symport generally. Both H+/sugar-and H+/K+ stoichiometries during the sugar-uptake have been reported to be exactly 1 : 1. This paper reports that the stoichiometries were enhanced dramatically by the addition of CaCl2 into the medium and by the high cell density of 200 $\mu$L pc/mL. The concentration of free Ca2+ ions in the cells increased significantly with cell density. It is suggested that the free Ca2+ ions are responsible for the change of stoichiometry of sugar transport system by regulation of H+ ion level of biomembrane.

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Design optimization of GaN diode with p-GaN multi-well structure for high-efficiency betavoltaic cell

  • Yoon, Young Jun;Lee, Jae Sang;Kang, In Man;Lee, Jung-Hee;Kim, Dong-Seok
    • Nuclear Engineering and Technology
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    • v.53 no.4
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    • pp.1284-1288
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    • 2021
  • In this work, we propose and design a GaN-based diode with a p-doped GaN (p-GaN) multi-well structure for high efficiency betavoltaic (BV) cells. The short-circuit current density (JSC) and opencircuit voltage (VOC) of the devices were investigated with variations of parameters such as the doping concentration, height, width of the p-GaN well region, well-to-well gap, and number of well regions. The JSC of the device was significantly improved by a wider depletion area, which was obtained by applying the multi-well structure. The optimized device achieved a higher output power density by 8.6% than that of the conventional diode due to the enhancement of JSC. The proposed device structure showed a high potential for a high efficiency BV cell candidate.

Protective Effect of Bcl-2 in NS0 Myeloma Cell Culture is Greater in More Stressful Environments

  • Tey, B.T.;Al-Rubeai, M.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.564-570
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    • 2005
  • In the present study, the protective effects of Bcl-2 over-expression in a suspension culture (without any adaptation) and spent medium (low nutrient and high toxic metabolite conditions) were investigated. In the suspension culture without prior adaptation, the viability of the control cell line fall to 0% by day 7, whereas the Bcl-2 cell line had a viability of 65%. The difference in the viability and viable cell density between the Bcl-2 and control cell lines was more apparent in the suspension culture than the static culture, and became even more apparent on day 6. Fluorescence microscopic counting revealed that the major mechanism of cell death in the control cell line in both the static and suspension cultures was apoptosis. For the Bcl-2 cell lines, necrosis was the major mode of cell death in the static culture, but apoptosis became equally important in the suspension culture. When the NS0 6A1 cell line was cultured in spent medium taken from a 14 day batch culture, the control cell line almost completely lost its viability by day 5, whereas, the Bcl-2 still had a viability of 73%. The viable cell density and viability of the Bcl-2 cell line cultivated in fresh medium were 2.2 and 2.7 fold higher, respectively, than those of the control cultures. However, the viable cell density and viability of the Bcl-2 cultivated in the spent medium were 8.7 and 7.8 fold higher, respectively, than those of the control cultures. Most of the dead cells in the control cell line were apoptotic; whereas, the major cell death mechanisms in the Bcl-2 cell line were necrotic.

MONITORING THE BAY OF BENGAL AS A BALLAST WATER EXCHANGEABLE SEA USING MODIS/AQUA

  • Kozai, Katsutoshi;Ishida, Hiroshi;Okamoto, Ken;Fukuyo, Yasuyo
    • Proceedings of the KSRS Conference
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    • v.1
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    • pp.483-486
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    • 2006
  • The study describes the monitoring of the Bay of Bengal as a ballast water exchangeable sea using MODIS/Aqua-derived diffuse attenuation coefficient (K(490)) synchronized with in situ ballast water sampling and analysis along the LNG carrier's route between Japan and Qatar from 2002 to 2005. Based on the relationship between K(490) and corresponding in situ plankton cell densities, the Bay of Bengal is recognized as a ballast water exchangeable sea to meet the regulation of ballast water performance standard of International Maritime Organization (IMO). Furthermore the Bay of Bengal with more than 200m depth and more than 200 nautical mile distance from shore is extracted based on the regulation of ballast water exchange area of IMO. However, an anomalously high K(490) area is found off the coast of Sri Lanka during the northeast monsoon in 2005, which corresponds higher cell densities than the criterion set by the regulation of IMO. The phenomenon of high cell density in the Bay of Bengal seems to be related with the phytoplankton bloom during the northeast monsoon. Seasonal and annual variability of phytoplankton bloom will be investigated to establish an early routing system for avoiding the high cell density area in advance.

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Metabolic Analysis of Poly(3-Hydroxybutyrate) Production by Recombinant Escherichia coli

  • WONG, HENG HO;RICHARD J. VAN WEGEN;JONG-IL CHOI;SANG YUP LEE
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.593-603
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    • 1999
  • Poly(3-hydroxybutyrate) (PHB) production by fermentation was examined under both restricted- and ample-oxygen supply conditions in a single fed-batch fermentation. Recombinant Escherichia coli transformed with the PHB production plasmid pSYLl07 was grown to reach high cell density (227 g/l dry cell weight) with a high PHB content (78% of dry cell weight), using a glucose-based minimal medium. A simple flux model containing 12 fluxes was developed and applied to the fermentation data. A superior closure (95%) of the carbon mass balance was achieved. When the data were put into use, the results demonstrated a surprisingly large excretion of formate and lactate. Even though periods of severe oxygen limitation coincided with rapid acetate and lactate excretion, PHB productivity and carbon utilization efficiency were not significantly impaired. These results are very positive in reducing oxygen demand in an industrial PHA fermentation without sacrificing its PHA productivity, thereby reducing overall production costs.

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High-level Production of Recombinant Human IFN-$\alpha2a$ with Co-expression of $tRNA^{Arg(AFF/AGA)}$ in High-cell-density Cultures of Escherichia coli

  • Shin, Chul-Soo;Hong, Min-Seon;Shin, Hang-Chel;Lee, Jeewon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.4
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    • pp.301-305
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    • 2001
  • The co-expression of the arg U gene in a double-vector expression system of recombi-nant Escherichia coli BL22(DE3)[pET-IEN2a+pAC-argU] significantly enhanced the production level of reconminant human interferon -$\alpha$2a(rhIFN-$\alpha$2a) in high cell density cultures, compared to a recombinant E. coli culture containing only the single expression vector, pET-IEN2a. The dry cell mass concentration increased to almost 100 g/L, and more than 4 g/L of rhIFN-$\alpha$2a was accumu-lated in the culture broth. Evidently, the synthesis of rhIFN-$\alpha$2a was strongly dependent on the pre-induction growtih rate and more efficient at a higher specific growth rate. The additional sup-ply of tRN $A^{Arg(AGG/AGA)}$ enhanced the expression level of the rhIFN-$\alpha$2a gene in the early stage of the post-induction phase, yet thereafter the specific production rate of rhIFN-$\alpha$2a rapidly de-creased due to severe segregational instability of plasmid vector pET-IEN2a. It would appear that the plasmid instability with only occurred to pET-IEN2a in the double vector system, was re-lated to the effect of translational stress due to the over expression of rhIFN-$\alpha$2a.

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Experimental Investigation of the Excited Xe Atoms Density in Ultra-high-resolution PDPs

  • Ishii, Keiji;Hirano, Yoshikuni;Murakami, Yukio
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.61-64
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    • 2008
  • We investigated experimentally the influence of the generation efficiency for excited Xe atoms on ultra-fine horizontal cell pitch AC PDPs. In the case of a 0.1 mm cell pitch, the sustain voltage increased; however, the generation efficiency was equal to or higher than for the conventional 0.22 mm cell pitch.

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Planar fuel cell design integrated with methanol reformer by using a high temperature membrane (고온형 멤브레인을 사용한 메탄올 개질 연료전지의 개질기 일체형 평판 설계)

  • Kim, Sung-Han;Jang, Jae-Hyuk;Gil, Jae-Hyoung;Lee, Hong-Ryul;Cha, Hye-Yeon;Ku, Bo-Sung;Jung, Chang-Ryul;Kundu, Arunaha;Miesse, Craig;Oh, Yong-Soo
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.11a
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    • pp.467-470
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    • 2006
  • For a mobile application such as cellular phone, micro fuel cells should be extremely compact and thin. RHFC can be an alternative solution because RHFC gives higher power density than DMFC and does not need ahydrogen storage vessel In this paper, RHFC using methanol fuel is made as a novel planar design without a PROX. Both reformer and cell are made closely in a same plate to share the heater of reformer with the cell. The PBI membrane is used in the cell. The reason is that high temperature of reformer can cause a performance drop when perfluorosulfonic acid membrane such as Nafion is used such a high temperature operation also guarantees the higher CO tolerance to MEA catalyst. The cell is designed as an air-breathing type which the cathode of the cell is opened to the air. The commercial Cu/ZnO/Al2O3 steam reformer catalyst is packed in reformer channel. The active area of MEA is $11.9cm^2$ and the peak power density was $27.5mW/cm^2$.

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Performance Characteristics of Li-ion Battery and Supercapacitor Hybrid Cell for High Power / Low Temperature Discharge (고출력/저온 방전을 위한 리튬전지와 슈퍼캐패시터 하이브리드 셀의 방전 거동 특성 연구)

  • Jang, Woojin;Hong, Seung-Chul;Hong, Jung-Pyo;Hwang, Taeseon;Oh, Joon-Suk;Ko, Sungyeon;Lee, Gaeun;Ahn, Kyunyoung;Kim, Hyunsoo;Suhr, Jonghwan;Nam, Jae-Do
    • Transactions of the Korean Society of Automotive Engineers
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    • v.21 no.6
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    • pp.49-57
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    • 2013
  • In this study, we fabricated a parallelly connected Li-ion battery/supercapacitor hybrid cell to combine the advantageous characteristics of Li-ion battery and supercapacitor, high energy density and high power density, respectively, and investigated its discharging characteristics over a wide temperature range from -40 to $25^{\circ}C$. At the initial state of discharging of the hybrid cell, the power was mostly provided by the supercapacitor and then the portion of the Li-ion battery was gradually increased. By installing a switching system into the hybrid cell, which controls the discharging sequence of Li-ion battery and supercapacitor, the maximum power was improved by 40% compared with non switching system. In addition at low temperatures, the power and discharging time of the hybrid cell were significantly enhanced compared to a battery-alone system. The hybrid cell is expected to be applied in electric vehicles and small domestic appliances that require high power at initial discharging state.