• Journal of the Korea Organic Resources Recycling Association
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• v.5 no.1
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• pp.25-32
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• 1997

The effect of levulinic acid (LA) and biosynthetic precursors of ${\delta}$-aminolevulinic acid (ALA) on the production of extracellular ALA was examined for the cells of soil derived Rhodospirillum rubrum N-1 belonged to the genus Rhodospirillaceae. The extracellular yield of ALA was increased to 23 fold (45 mg/l) from the basal condition (Lascelles' medium without L-glutamate) by successive addition of LA at initial (10 mM) and mid-log stage (30 mM) of cell cultivation. In addition to initial/mid-log mutual supplementations of LA (10 mM/30 mM) and glutamate (30/30 mM), respectively, by means of alternative feeding 10 mM $C_4$-precursors at mid-log phase of culture the extracellular ALA content was reached to 75 mg/l (40 fold).

• Korean Journal of Environmental Agriculture
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• v.15 no.4
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• pp.488-493
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• 1996

This study was conducted to find out the residual aspect and the effect of quizalofop-ethyl on microorganisms used to control broad-leaf weeds at Yeongok soil series, the experiment field, Chonnam R.D.A. and Namwon soil series, the experiment field, Jeju R.D.A. More than 60 percent of quizalofop-ethyl treated in soil was degraded within 7 days. The degradation of quizalofop-ethyl in soil increased rapidly with incubation temperature. The half-life of quizalofop-ethyl in soil was 15 days(Yeongok series) and 16 days(Namwon series). The number of microorganisms in soil treated with quizalofop-ethyl decreased prominently with incubation time. But the number of Fusarium did not reduce in comparison with that of other soil microorganisms. Therefore, it was thought that the decomposition of quizalofop-ethyl in soil was affected by $Fusarium^2$.

• Korean Journal of Environmental Agriculture
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• v.16 no.1
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• pp.67-71
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• 1997

This experiment was conducted to test the allelopatic activity on germination and early seedling growth of weed species by barley and sorghum plants residues. The fresh barley extraction inhibited the germination and early seedling growth of weeds, Echinochloa crus-galli, Sataria viridis, and P. oleracea. As the extract concentration increased, the germination and early seedling growth of weeds was significantly inhibited. The water extraction of dried barley and sorghum residues also ehibited the strong inhibition effect on germination and Barley seedling growth of weeds. Digitaria sanguinalis, Siegesbechia pubescens, Sectaria viridis, P. oleracea, E. crus-galli. In the dried barley and sorghum residues mixture into the vermiculite, as the dried residue concentration ncreased, emergence percentage, length of shoot and root of weeds, D. saguinalis, S. viridis, S. pubescens, Ammaranthus lividus, and Solanum nigrum, was significantly inhibited. More than 10% concentration of dired residue caused 80% emergence percentage and growth inhibition. From this study, we conclude that barley and sorghym weeds. These results suggest that barley and sorghum has some possibility to control some weed species like natural herbicide.

• Korean Journal of Environmental Agriculture
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• v.4 no.2
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• pp.118-125
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• 1985

To establish an evaluation system of aquatic toxicity of chemicals at no-effect level, flow through and early life stage toxicity test were performed on a freshwater fish, the medaka (Oryzias latipes). The characteristics of medaka as a bioassay organism for the chronic toxicity test were discussed. Maximum acceptable toxicant concentration(MATC) of butachlor for the madaka in soft water was estimated using survival, growth, and reproduction as indicators of toxic effects. During a 3-month period, the fry of medaka were exposed to butachor concentrations ranging from 0.16 to 0.0l mg/liter and the DO concentration, temperature, and pH in the exposure chamber were measured to check the test condition. The highest concentration showed slight decrease of growth rate in medaka and reduced hatchability of spawning egg. Survival, growth, and reproductive success of adults in butachlor concentration of 0.04 and 0.01 mg/liter were not different from those of the control. The MATC was estimated to be between 0.04 and 0.16 mg/liter for medaka.

• Applied Biological Chemistry
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• v.41 no.1
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• pp.99-103
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• 1998

Triazolopyrimidine sulfonanilide (TP) derivative is one of excellent herbicide compounds. We have synthesized three classes of a new sulfonamide derivative (TPP) as Acetolactate synthase (ALS) inhibitors, in which the benzene ring in TP skeleton was converted to substituted pyrimidyl ring and examined their inhibitory activities on barley for ALS. $I_{50}$ values of the inhibitors ranged from 0.005 to 2 mM. Comparing the $I_{50}$ value of each class of TPP derivatives, the substituents in pyrimidine and triazolopyrimidine ring were found to affect the degree of ALS inhibition. TPP with substituted methyl group in pyrimidine ring showed higher inhibitory activity than that with methoxy group, while the substitution of the cyclopentano group in triazolopyrimidine ring gave very large inhibitory activity than that of methyl group. The present study established that variation of the electron density by substitution at heterocyclic ring is a very important factor for ALS inhibition, but showed no dependence on steric effect by substituents.

• Applied Biological Chemistry
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• v.38 no.4
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• pp.283-288
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• 1995

This study was carried out to investigate the toxic action of herbicide, paraquat(1,1'-dimethyl-4,4'-bipyridylium-dichloride), against microorganisms. The toxic effect of paraquat was observed mainly using Escherichia coli(KCTC 1039), as follows; Growth of aerobic microorganisms which comprise 4 strains of bacteria and 2 strains of yeast and 4 strains of mold was inhibited drastically in the presence of 1.0mM paraquat But the growth of anaerobic bacteria was not affected by the chemical. When actively growing cells of E. coli were exposed to the paraquat at the concentration higher than 1.0 mM, they rapidly lost their ability to form colony and clearly formed inhibitory zone by well test More than 50% of the cells were killed by 1.0 mM paraquat treatment, even at immediate addition of paraquat to the medium.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.40-46
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• 2013

For a case study of suspected paraquat intoxication, we developed a simple and rapid method of $^1H$ qNMR to determine the mili-molar amount of paraquat in postmortem blood samples. There were no interfering signals from endogenous compounds in the chemical shift of paraquat and diquat (internal standard). The amount of sample used ranged from 0.25 mM to 10.0 mM. Diquat, which has similar physicochemical properties with paraquat, was chosen as an internal standard. The NMR experimental conditions, relaxation delay time and CPMG spin-echo pulse sequence were optimized. The developed method was validated in terms of specificity, accuracy, precision, matrix effect, recovery, limit of detection (LOD), and low limit of quantification (LLOQ). The proposed qNMR method provided a simple and rapid assay for the identification and quantification of the quaternary ammonium herbicide, "paraquat" in postmortem blood samples. This method was tested by using the blood from the heart of a man who was intoxicated with paraquat. In this particular case, the level of paraquat was 1.07 mM in the blood. For the determination of quaternary ammonium herbicides, qNMR could also be used to provide a better understanding of the currently available techniques.

• Korean Journal of Weed Science
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• v.17 no.1
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• pp.80-93
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• 1997

Allelopathic compounds as naturally occurring herbicide have originally reported from local vegetation since B.C. 300. These compounds are known as secondary plant metabolites which released from plants into the environment often attract or repel, nourish or poison other organisms. In recent, many natural plant allelochemicals be used to attempt to biologically or ecologically control weed among worldwide weed scientists. Some allelochemicals have also used as fungicides, insecticides, and nematodicides, and were less than man-made agrochemicals to damage the global ecosystem. It makes efficient use of resources internal to the farm, relies on a minimum of purchased inputs. Some scientists selected for allelopathic activity when breeding weed-controlling cultivars of rice, sorghum, cucumber, surflower etc. Thus, this paper is focused on allelopathic compounds isolated from cultivated crop with the high potential of prospective herbicides. The most environmentally acceptable and sustainable approach to utilization of allelopathy for weed control is to develop plant cultivars with proven allelopathic characteristics. In rice accessions, there are 60 cultivars/lines which have known as allelopathic activity and some of these cultivars control weed more less 90% within certain radius of activity. These accessions are originated from 15 countries including Korea, Japan, USA, India, Philippines, Indonesia, Laos, Taiwan, Afghanistan, Mali, Pakistan, Colombia, Egypt, China, and Dom. Rep. From these cultivars, the most common allelopathic compounds identified in rice are p-Hydroxybenzoic, Vanillic, p-Coumaric, and Ferulic acids. In addition, allelopathic lines of the following crop have shown inhibition of weed growth : beet (Beta vulgaris), lupin(Lupinus spp.), com(Zea mays), Wheat(Triticum aestivum), oats(Avena spp.) peas(Pisum sativum), barley(Hordeum vulgare), rye(Secale cereale), and cucumber(Cucumis sativus). Thus, future allelopathy research must be designed its potentially phytotoxic propertices and the ecotoxic features of the allelochemicals from release to degradation ; its ecological sustainability, its allelopathic effect in early growth. stages, and selectivity properties in combination with chemical stages, and selectivity properties in combination with chemical concentrations. Also, research approach in allelopathy might be screened for highly allelopathic germplasm collection of crops, the idea being to ultimately transfer this agronomic character into improved cultivars by either conventional breeding or other genetic transfer techniques.

• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.827-841
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• 2019

The effect of genetically engineered rice Bt-9 on the diversity and abundance of plant-dwelling insects and spiders was tested under field conditions. Genetically engineered rice Bt-9, expressing mCry1Ac1 from Bacillus thuringiensis, confers resistance to rice leaf roller (Cnaphalocrocis medinalis) and provides tolerance to the herbicide glufosinate (PPT). The study compared Bt-9 and two non-GM reference varieties, Ilmi-byeo and Dongjin-byeo, at LMO isolated fields in Gunwi (Kyungpook National University) and Jeonju (National Institute Agricultural Sciences) in Southern Korea in 2016 - 2017. A total of 40,817 individuals from 62 families and 11 orders were collected from the two living modified organism (LMO) isolated fields. From the three types of rice fields, a total of 13,982, 14,105, and 12,730 individuals from the Bt-9, Ilmi-byeo and Dongjin-byeo were collected, respectively. Throughout the study, the analysis of variance indicated no significant differences (p < 0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects were similar. The data on insect species population densities were subjected to principal component analysis (PCA), which did not distinguish among the three varieties, Bt-9 and the non-GM, reference cultivars, during the cultivation years. However, the results of the PCA analysis were completely divided into four groups based on the yearly survey areas. Therefore, there was no evidence for a negative impact of Bt-9 on the above-ground insects and spiders.

• Journal of Plant Biotechnology
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• v.1 no.1
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• pp.46-55
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• 1999

The expressed sequence tags(ESTs) from immature seed of rice, Oryza sativa cv Milyang 23, were partially sequenced and analyzed by homology. As of 1998, the partial sequences of about 6,600 cDNA clones were analyzed from normal and normalized immature seed cDNA libraries. About 2,200 ESTs were putatively identified by BLASTX deduced amino acid sequence homology analysis. About 20% of them were putatively identified as storage proteins. Also the clones were highly homologous to genes involved particularly in starch biosynthesis, glycolysis, signal transduction and defenses. Compared to 35% of redundancy in the ESTs of normal cDNA library, that from the substracted library was 15%. The Korea Rice Genome Network is maintained to provide the updated information of sequences, their homologies and sequence alignments of ESTs. For the stable expression of transgene in rice, diverse vectors were developed for overexpression, targeting and gene dosage effect with transit peptides (Tp) and matrix attachment region (MAR) sequence from chicken lysozyme locus. The rice calli were transformed via Agrobacterium tumefaciens LBA4404(pSB1) with the triparental mating technique and selected by herbicide resistance. The green fluorescent protein(GFP) gene in expression vector under the control of rbcS promoter-Tp was overexpressed upto 10 % of the total soluble protein. In addition, the Tp-sGFP fusion protein was properly processed during translocation into chloroplast. The expression of sGFP in the presence of MAR sequences was analyzed with Northern and immunoblot analysis. All the lines in which sGFP transgene with MAR sequence, showed position independent and copy number-dependent expression, while the lines without MAR showed the varied level of expression with the integration site. Thus the MAR sequence significantly reduced the variation in transgene expression between independent transformants.