• Toxicological Research
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• 제6권1호
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• pp.109-119
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• 1990

Ro09-0198, a cyclic peptide isolated from culture filtrates of Streptoverticillium griseoverticillatum, induced lysis of erythrocytes. Ro-09-0198-induced hemolysis was temperature-dependent and the sensitivity of hemolysis differed greatly among animal species. Preincubation of the peptide with phosphatidylethanolamine reduced the hemolytic activity, whereas other phospholipids present in erythrocytes in nature had no effect. A study of the structural requirements on phosphatidylethanolamine necessary for interaction with the peptide indicates that Ro09-0198 recognizes strictly a particular chemical structure of phosphatidylethanolamine: dialkylphosphoethanolamine as well as 1-acylglycerophosphoethanolamine showed the same inhibitory effct on hemolysis induced by Ro09-0198 as diacylphosphatidyl-ethanolamine, whereas phosphoethanolamine gave no inhibitory effect. Neither phosphatidyl-N-monomethylethanolamine nor alkylphosphopropanolamine had an inhibitory effect. Proton resonances of the peptide were observed in dimethyl sulfoxide solution in the presence of 1-dodecanoyl-sn-glycerophosphoethanolamine. This peptide caused permeability increase and aggregation of liposomes containing phosphatidylethanolamine. A glycerol backbone and a primary amino group of phosphatidylethanolamine are necessary for interaction with Ro09-0198 to cause membrane damage. Ro09-0198 induced a selective permeability change on liposomes. Glucose and umbelliferyl phosphate were effluxed significantly, but sucrose was only slightly permeable and inulin could not be released. Platelet aggregation and serotonin release simultaneously induced by Ro09-0198. Addition of peptide to rat platelet, loaded with the fluorescent $Ca^{++}$ chelator quin-2, caused immediate rise in cytosolic free $Ca^{++}$ to liposomal membrane containing phosphatidylethanolamine was observed dose dependently.

• Childhood Kidney Diseases
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• 제6권1호
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• pp.120-122
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• 2002

IgA신병증으로 말기 신부전에 도달한 B형 혈액형의 9세 남아가 O형 혈액형의 아버지로부터 신이식을 시행받았다. 환아는 16개월 간 복막 투석 중이었으며 이전에 수혈받은 병력은 없었다. 이식 9일째 혈관내 용혈이 발견되었으며 항 B 항체 형성이 확인되었다. 저자들은 공여자의 신장과 함께 이동한 림프구가 일으킨 자가 면역 용혈성 빈혈로 진단하고 사이클로스포린 투여를 중단하였다. 용혈성 빈혈은 회복되었으나 이식 18일째 이식 신 기능 저하가 발견되어 이식 신생검을 시행하여 급성 거부를 확인하고 스테로이드 충격 요법을 시행하였다. 저자들은 ABO 부적합 신이식 후 발생한 용혈성 빈혈 1례를 겅험하여 보고하는 바이다.

• Journal of Chest Surgery
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• 제25권12호
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• pp.1556-1562
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• 1992

Forty two consecutive patients who had had valve replacement with St. Jude Medical prosthesis were studied on a view point of intravascular hemolysis. Patients were consisted of 14 mitral valve replacement, and 7 aortic valve replacement, and 21 double, mitral and aortic, valve replacement. Serum LDH, indirect bilirubin, GOT, hemoglobin levels and ret-iculocyte count were pursued in postopeative 1st day, 3rd day, 7th day, 14th day and 21th day. Postoperatively, all patients were not detected paravalvular leakage on the ech-ocardiographical study. The patients with double valve replacement revealed higher levels of LDH on postopeative 14th day[P<0 05] than those with single valve replacement. Among the patients with single valve replacement, the patients with aortic valve replacement revealed slightly higher levels of entire postopeative data, but considered insignificant. There was correlation between the severity of hemolysis and the size of replaced aortic valve. In the postoperative LDH levels, the patients with small sized-aortic valve[less than 21mm in diameter] replacement revealed higher levels of postoperative 3rd day, 7th day and 14th day than those with large size[more than 23mm in diameter]. The patients with high level LDH of greater than 800 WU /L on postoperative 7th day were 61.9%[26 of 42]. The high LDH frequency of DVR was 71.4%[15 of 21], MVR 50.0%[7 of 14] and AVR, 57.1%[4 of 7]. The level of LDH declined gradualiy thereafter through postoperative 3 weeks. In conclusion, intravascular hemolysis after prosthetic valve replacement was dependent on position of valve replacement and size of valve. And this study supports the conventional valve selection and usage in our hospital. The patients with subclinical hemolysis after valve replacement should be placed on a close observation.

• 한국식품위생안전성학회지
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• 제10권1호
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• pp.29-32
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• 1995

Inclusion complex of omeprazol with $\beta$-Cyclodextrin and Hydroxypropyl-$\beta$-Cyclodextrin were prepared by coprecipitation and freeze-drying method respectively. Effects of these inclusion complex on RBCs were monitored with a spectrophotometer by the method of Kahan et al. and the mutagenic activity based on the Ames plate incorporation test in the presence and absence of liver microsomal enzyme(S9 fraction) using Salmonella typhimurium TA98 and TA100. The RBCs hemolysis and mutagenic activity of these complex were not detected.

• 대한약리학회지
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• 제4권1호
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• pp.9-16
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• 1968

The author studied the hemolytic action of phenothiazine derivatives such as promazine, prochlorper azine, perphenazine and thiethylperazine on rabbit erythrocytes, and obtained the following results: 1. Promazine, prochlorperazine, perphenazine and thiethylperazine caused hemolysis in vitro in the following order: Thiethylperazine>perphenazine>prochlorperazine>promazine. 2. Cholesterol inhibited the hemolytic action of prochlorperazine, perphenazine and thiethylperazine, but had no effect on promazine hemolysis. 3. Dextrose, albumin and blood plasma protected erythrocytes against promazine, prochlorperazine, perphenazine and thiethylperazine. 4. The intravenous injection of promazine, prochlorperazine, perphenazine and thiethylperazine caused hemolysis in the same order as they did in vitro.

• International Journal of Fluid Machinery and Systems
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• 제7권4호
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• pp.142-150
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• 2014

In the cardiopulmonary support system with an ECMO (extracorporeal membrane oxygenation), a higher pump head is demanded for a blood pump. In order to realize a blood pump with higher pump head, higher anti-hemolysis and thrombosis performances, a study on the development of unprecedented multistage blood pump was conducted. In consideration of the application of the blood pump for pediatric patients, a miniature two-stage centrifugal blood pump with the impeller's diameter of 40mm was designed and the performance was examined in experiments and computations. Some useful knowledge for a design of the blood pump with higher anti-hemolysis and thrombosis performances was obtained.

• Journal of Ginseng Research
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• 제33권4호
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• pp.305-310
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• 2009

Phenanthrene, one of the polycyclic aromatic hydrocarbons, has been known to be toxic to the environment. In this investigation, the protective effect of red ginseng on phenanthrene-induced oxidative DNA damage was evaluated using Comet assay in A549 cells. Red ginseng's cytoprotective effect on phenanthrene-induced hemolysis was also investigated. This study's findings show that oxidative DNA damage and hemolysis were significantly prevented by red ginseng treatment. Notably, it was found that pulverizing red ginseng into ultra-fine particles even enhanced its protective effects against DNA damage and hemolysis. The results suggest that particle size reduction seems to effectively enhance red ginseng's pharmacological efficacies.

• Archives of Pharmacal Research
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• 제18권6호
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• pp.402-409
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• 1995

The effects of calcium channel blockers (CAB's) verapamil, diltiazem and nicardipine, on erythrocyte ghost membranes have been studied. Using the fluorospectroscopic method, it was observed that the fluidity of the inner layer of ghost membranes was increased with an increase of drug concentrations but did not any changes in the fluidity of the outer layer. These drugs showed protectuve effect against hypotonic hemolysis of erythrocytes. Thus, the expansion of surface area in response to corpuscular volume of erythrocytes in the presence of CAB's is seemed to play an important role in protecting hypotonic hemolysis of erythrocytes.

• Macromolecular Research
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• 제11권1호
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• pp.19-24
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• 2003

Polymeric gene delivery system attracts profound attention as it shows less toxicity, versatility, and reasonable gene expression efficiency. Terplex system, a synthetic biopolymeric gene delivery system consisting of stearyl poly-L-lysine (stearyl-PLL) and low density lipoprotein (LDL) was evaluated for its body distribution of gene expression of exogenously administered pDNA after tail-vein injection in mice. Kidney and spleen are two major organs with highest gene expression, whereas liver and heart showed marginal gene expression among the organs examined. Hemolytic effect of the terplex system was evaluated using human red blood cells, where terplex system did not cause significant hemolysis at the concentrations above the experimental ranges, although unmodified PLL or stearyl-PLL without LDL did. Serum stability of terplex system against enzymatic degradation was also significantly enhanced, presumably due to the steric stabilization from the polymers. Based on these findings and along with its high in vitro transfection efficiency, terplex system could serve as a safe and efficient polymeric gene delivery system with many applications for the in vivo gene therapy.

• 한국동물위생학회지
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• 제28권2호
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• pp.113-119
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• 2005

We surveyed the distribution of Listeria spp in intestinal contents of slaughtered cattle from Daegu between March and October 2003. Fourteen Listeria spp were isolated from a total of 100 samples. Two samples contained only L innocua and other six samples contained both L monocytogenes and L innocua. Of the 99 samples positive to esculin reaction in Fraser broth, Listeria spp were isolated only from $8\%$ of the samples. Three selective plating medium were examined for detection of Listeria species including Enhanced hemolysis agar, Oxford agar and Palcam agar, It was found that Enhanced hemolysis agar was more effective than Oxford agar and Palcam agar, and that L monocytogenes needed 48 hour growth to give positive reaction.