• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4285-4289
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• 2015

Purpose: Blood hemoglobin levels are known to influence response to radiotherapy. This retrospective analysis compared the effect of hemoglobin levels upon response to radiation among patients treated with radiation alone (by accelerated hyperfractionated radiotherapy) versus those treated with concurrent cisplatin chemoradiotherapy. Materials and Methods: Among patients treated for locally advanced carcinoma of the cervix (LACC) during 2009-10, a total of 60 fulfilled the eligibility criteria. In this time frame, external beam radiotherapy was delivered with either concurrent chemoradiotherapy (CRT, n=31) (45Gy over 25 fractions, with weekly cisplatin at 40mg/m2), or with accelerated hyperfractionated radiotherapy (AHRT, n=29) (20Gy over 10 daily fractions over the first two weeks, followed by 30Gy over 20 fractions over the next two weeks, with two fractions of 1.5Gy per day, without the use of chemotherapy). Mean weekly hemoglobin (MWH) levels of all patients were calculated as the arithmetic means of weekly recorded blood hemoglobin levels. As per MWH, patients in both of the AHRT or the CRT groups were classified into two subgroups-those with MWH between 10-10.9g/dL, or with MWH>11g/dL. Complete response (CR) to external beam RT phase (prior to brachytherapy) was declared after clinical examinations and computed tomography. The CR rate was noted for both MWH sub-groups within each of the AHRT and CRT groups. Results: Within the AHRT group, patients with MWH>11g/dL had a much better CR rate in comparison to those with MWH:10-10.9g/dL (80% vs. 21.1%) which was statistically significant (p 0.0045). Within the CRT group, there was no significant difference in the outcomes within the MWH>11g/dL and MWH:10-10.9g/dL sub-groups (CR rates of 80% vs. 61.9%, p=0.4285). Conclusions: The importance of maintaining a minimum hemoglobin level of 11g/dL during RT is much greater for patients treated with RT alone, than for patients treated with concurrent chemoradiotherapy. Enhanced haemoglobin levels during RT may to an extent negate the ill-effects that may otherwise arise due to non-use of concurrent chemotherapy.

• Journal of Chest Surgery
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• 제25권7호
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• pp.693-701
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• 1992

Extracorporeal cardiopulmonary bypass[CPB] has been associated with a wide variety of hematologic derangements, including a transient deformation and hemolysis of red blood cells[RBCs], which is supposed to be due to mechanical trauma and/or metabolic alterations. Since membrane integrity is, in part, maintained by energy requiring process, inadequate function of erythrocyte glycolytic pathway, which is inevitalble during CPB, may cause depletion of high energy phosphate pool and result in hemolysis. The authors performed an investigation to assess whether administration of Fructose-l, 6-diphsphate [FDP], which has been known to enhance intracellular glycolytic activities, could counteract erythrocyte hemolytic events caused by CPB. Sixty pateints with cyanotic congenital heart diseases, who underwent open heart surgery under CPB longer than 60 minutes, were randomly divided into two groups depending on whether use of FDP[Group FDP] or not[Group Control]. The age, sex, CPB time, preoperative hemoglobin level, disease entities were all similar[Table 1], and membrane type oxygenators were used in all patients. In Group, FDP, a dose of 250mg/kg body weight of FDP was administered by intravenous dripping every 12 hours from the morning of the operation to postoperative 48 hours, To demonstrate the degree and pattern of hemolysis of erythrocyte, reticulocyte count, indirect /direct bilirubin, haptoglobin, plasma hemoglobin, lactate dehydrogenase were measured every 12 hours from the time of cessation of CPB to 48 hours and RBC morphologic study, osmotic fragility test were done every 24 hours. All parameters revealed less hemolytic in group FDP [Fig. 1~5], though the differences between two groups were not significant, except plasma hemoglobin, lactate dehydrogenase changes. A pattern of sequential changes of plasma hemoglobin, lactate deh-ydrogenase showed the highest level at the time of CPB stop and abrupt decrease in following 24 hours in both groups, and statistically significant differences were demonstrated in group FDP at least for the first 12 hours postoperatively[p<0.05]. The authors conclude that they can expect the benificial effect of FDP on the maintenance of membrane stability of RBC probably by energy enhancement during the shock status of CPB, but FDP could not completely prevent the damaging effect on RBC by cardiopulmonary bypass

• 한국환경보건학회지
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• 제30권4호
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• pp.301-307
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• 2004

1,3-butadiene(DB) is a well-established rodent carcinogen, and a probable carcinogen to humans. This study was investigated the formation of hemoglobin adduct in ICR female mice inhaled with BD for 3 weeks (5 hr/day, 5 days/week). Body weights of mice were significantly low from onward day 4 or 9 of exposure comparing the control. Hemoglobin adducts formed by DB exposure were (N-2-hydroxy-3-butenyl) valine (HB Val adduct) and (N-2,3,4-trihydroxy-butyl)valine(THB Val adduct). The levels of HB Val adducts were 1.8, 3.7 and 6.2 pmol/mg globin for the 500 ppm BD inhalation group, and 5.7, 7.4 and 16.0 pmol/mg globin for the 1000 ppm BD inhalation group, when observed on the $1^{st},\;2^{nd},\;and\;3^{rd}$ week after inhalation exposure, respectively. The levels of THBVal adducts were 32.0, 42.0 and 55.0 pmol/mg globin for the 500 ppm DB inhalation group, and 67.8, 72.7 and 83.5 pmol/mg globin for the 1000 ppm BD inhalation group, when observed on the $1^{st},\;2^{nd},\;and\;3^{rd}$ week after inhalation exposure, respectively. Ratios of THBVal and HBVal adducts were higher at earlier exposure period and lower concentration. Ratios on the $1^{st},\;2^{nd},\;and\;3^{rd}$ week were 17.8, 11.4 and 8.87 for the 500 ppm BD inhalation group, and 11.9, 9.8 and 5.2 for the 1000 ppm BD inhalation group, respectively. In conclusion, THB Val and HB Val adducts were the important hemoglobin adducts in ICR female mice inhaled with BD, and the latter was more appropriate biomarker than the other for biological monitoring of BD exposure.

• 대한임상검사과학회지
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• 제54권1호
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• pp.1-8
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• 2022

Erythrocyte sedimentation rate (ESR) evaluation is a useful tool for monitoring disease activity in various inflammatory and non-inflammatory conditions. ESR is known to be influenced by a multitude of confounding factors. The present study aimed to assess the possible determinants of the ESR and its relationship with various risk factors involved in ischemic stroke. ESR and other hematological and biochemical parameters were investigated in 163 ischemic stroke patients (107 males and 56 females) selected based on imaging techniques including magnetic resonance imaging (MRI) or computed tomography (CT) scans. Statistical analysis was performed using the SPSS 16.0 software. Linear regression analysis showed a significant inverse relationship of hemoglobin (Hb) and hematocrit or packed cell volume (PCV) (P<0.001 for females; P<0.01 for males) with the ESR. It was observed that the red blood cell (RBC) count was not strongly correlated with the ESR (P<0.05 for both males and females). It was also observed that sex significantly affected the variables determining the ESR levels, whereas age had no effect. Gender differences were also observed with respect to Hb, RBC, PCV, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and ESR. The possible determinants of higher ESR levels in ischemic stroke may be sex, Hb, hematocrit, and RBC count, but the role of other clinical and laboratory parameters cannot be underestimated.

• Journal of Acupuncture Research
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• 제21권1호
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• pp.99-110
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• 2004

Objective: The purpose of this study was to examine the different effects on labor pain, length of delivery time, and hemoglobin for primipara women treated by San-Yin-Jiao(SP-6) acupressure of 15 minutes or 30 minutes. Methods: The design of this study was a randomized clinical trial with two experimental groups and one control group, the participants included primiparas who underwent vaginal delivery. Data were collected using a structured questionnaire consisting of general characteristics with a subjective labor pain scale with VA.S, and length of delivery time and hemoglobin were collected in the medical record. Data was analyzed using descriptive statistics, x2- test, t-test, and ANCOVA. Results: There was statistically significant lower labor pain in the group of 30 minutes SP6 acupressure than the control group(t=7.733, p=.007), and there was statistically significant short length of delivery time in the group of 15 minutes SP6 acupressure than the control group(t=-2.230, p=.030). Conclusions: Even though we could not found a consistent effect with the SP6 acupressure, It was found that there was effect on shortening length of delivery time by 15 minutes of SP6 acupressure or lessening labor pain by 30 minutes of SP6 acupressure without risk of blood loss. It is necessary to replicate the study with a larger number of participants to find a consistent result.

• 대한한의학회지
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• 제37권2호
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• pp.104-109
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• 2016

Objectives: A 74-year-old male patient with unresectable advanced gastric cancer (clinical initial stage T3N+, Borrmann type III) admitted due to gastric bleeding at tumor site. On first admission day, hemoglobin level was 5.7g/dl and performance status was grade 3 according to Eastern Cooperative Oncology Group Performance Status(ECOG-PS). After performing red blood cell transfusion as an emergency treatment, hemoglobin level was increased up to 9.5g/dl. However, bleeding of oozing site was continued. For hemostasis, decoction of notoginseng radix (30g/day) was administered since day 7 after admission. The dose was elevated to 40g/day after hemoglobin level was decreased to 6.5g/dl on day 11. Since then, melena was stopped and hemoglobin level was maintained over 9.1g/dl. This case shows the hemostasis effect of decoction of notoginseng radix on gastric bleeding in unresectable advanced gastric cancer.

• 대한수의학회지
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• 제34권3호
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• pp.487-492
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• 1994

The study was conducted to investigate the phenotypes of blood proteins, hemoglobin and albumin, of 104 German shepherds in Dag-gu, Yae-chum Kim-hae and Kwang-ju area by the starch gel electrophoresis. The results obtained were summarized as follows: 1. The hemoglobin phenotypes were observed three(HbBB, HbBb and Hbbb). Fast migrating bands were same and slow migrating bands were divided by heavy(HbBB), light(HbBb) and non-stained(Hbbb). The frequencies of appearance in HbBB, HbBb and Hbbb were 17.31%, 51.92% and 30.77%, respectively. 2. The hemoglobin phenotypes were controlled by two allelic genes, $Hb^b$ and $Hb^B$. The gene frequencies were calculated at 0.567 in $Hb^B$ and 0.433 in $Hb^A$. 3. The albumin phenotypes were observed three(AlbFF, AlbSS and and AlbFS), which were divided by fast migrating band(AlbFF), slow migrating band(AlbSS) and mixed migrating band(AlbFS). The frequencies of appearance in AlbFF, AlbFS and AlbSS were 5.77%, 28.85% and 65.38%, respectively. 4. The albumin phenotypes were controlled by two alleic genes, $Alb^F$ and $Alb^S$. The gene frequencies were calculated at 0.202 in $Alb^F$ and 0.798 in $Alb^S$.

• Reproductive and Developmental Biology
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• 제28권3호
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• pp.161-166
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• 2004

체외수정 후 체외배양48시간에 난구세포의 제거 유ㆍ무에 따라 CR/sub 1aa/ 배양액에 homoglobin을 0, 1, 5 ㎍/㎖를 첨가한 구의 상실배기 이상 체외발육성 적은 난구세포를 제거한 구에서 23.8%, 33.3% 및 26.8% 였으며, 난구세포를 제거하지 않은 구에서는 각각 39.5%, 54.8% 및 48.8%로서 난구세포를 제거하지 않은 구에 Hb를 1 ㎍/㎖를 첨가한 구가 여타구보다 통계적으로 높은 결과를 얻었다(P<0.05). 체외수정 후 체외배양 96시간 후 난구세포를 제거 유ㆍ무에 따라 Hb를 0, 1, 5 ㎍/㎖를 첨가하였을 때, 상실배기 이상 체외발육성적은 난구세포를 제거한 구에서 각각 28.6%, 46.2% 및 39.1%였으며, 난구세포를 제거하지 않은 구에서는 각각 33.9%, 67.2% 및 46.0%로서, 난구세포를 제거하지 않은 구에 Hb를 1 ㎍/㎖를 첨가구가 여타구에 비해 통계적으로 유의하게 높게 나타났다(P<0.05). CR/sub 1aa/ 배양액에 L-NAME를 0, 10, 50 및 100 mM을 첨가한 구에서 상실배 이상 발육된 체외발육성적은 각각 55.6%, 64.9%, 58.8% 및 66.7%로써 L-NAME 첨가구가 무첨가구에 비해 커다란 차이가 나타나지 않았다(P>0.05). 모든 처리구에 배반포까지 발육된 체외수정란의 세포수에는 커다란 차이가 인정되지 않았다.

• Applied Biological Chemistry
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• 제44권3호
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• pp.143-147
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• 2001

Hemoglobin(Hb) 가수분해도(degree of hydrolysis, DH)를 간편하고 신속하게 측정하고자 보편적으로 이용되는 TNBS법(trinitrobenzene sulfonic acid method)에 의한 가수분해도와 osmometer를 이용한 방법의 상관관계를 효소반응 조건에 따라 조사하였다. 다양한 ph 범위$(pH\;7.5{\sim}10.0)$에서 DH에 대한 두 방법의 상관계수($R^2$값)는 $0.974{\sim}0.991$로 매우 높은 값을 보였으며, 각각 측정한 DH 사이의 비율($DH_{OSM}/DH_{TNBS}$은 $1.438{\sim}1.656$의 범위에 있었다. Hb을 가수분해하여 heme-iron을 얻기 위한 특정 반응조건인 pH 7.5, $50^{\circ}C$에서 Esperase와 Fla-vourzyme을 각각 사용한 경우와 Esperase와 Flavourzyme을 혼용한 경우에서도 두 방법간의 DH 비율은 효소의 종류 및 처리방법에 무관하게 1.658을 나타내어 Esperase만을 사용한 경우와 매우 유사하였고 두 방법간의 상관계수는 0.982이었다. 따라서, 두 방법간의 상관관계식으로부터 osmometer를 이용한 Hb의 가수분해도를 간편하고 신속하게 측정할 수 있었다.

• Journal of Dental Anesthesia and Pain Medicine
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• 제21권5호
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• pp.471-474
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• 2021

Methemoglobinemia is rare. It is classified into two types: congenital methemoglobinemia and acquired methemoglobinemia. Methemoglobin is incapable of binding oxygen, leading to complications such as cyanosis, dyspnea, headache, and heart failure. In the present case, a 35-year-old man with congenital methemoglobinemia underwent general anesthesia for thyroidectomy. The patient was diagnosed with hemoglobin M at 7 years of age. Ventilation was performed with FiO₂ 1.0. Arterial blood gas analysis showed that the pH was 7.4, PaO₂ 439 mmHg, PaCO₂ 40.5 mmHg, oxyhemoglobin level of 83.2%, and methemoglobin level of 15.5%. The patient had a stable course, although cyanosis was observed during surgery.