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Effects of Nitric Oxide Scavenger and Inhibitor on the Development of Bovine IVM/IVF Embryos  

Jang H. Y. (College of Animal Resources Science, Kangwon National University)
Kim J. T. (College of Animal Resources Science, Kangwon National University)
Park C. K. (College of Animal Resources Science, Kangwon National University)
Cheong H. T. (College of Animal Resources Science, Kangwon National University)
Kim C. I. (College of Animal Resources Science, Kangwon National University)
Yang B. K. (College of Animal Resources Science, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.28, no.3, 2004 , pp. 161-166 More about this Journal
Abstract
This study was designed to evaluate the effects of nitric oxide scavenger (hemoglobin) and inhibitor (L-nitro-L-arginine methyl ester; L-NAME) with or without cumulus cell on the development of bovine IVM/IVF embryos. When CR/sub 1aa/ medium were supplemented with different dosage (lug/m, 5ug/m and 10ug/ml) of hemoglobin at 48hrs for in vitro culture, the proportion of embryos developing beyond morulae stage in 0, 1ug/ml and 5ug/ml with or without cumulus cell were 23.8%, 33.3 % and 26.8%, and 39.5%, 54.8% and 48.8%, respectively. There was a significantly difference the developmental rate of 1ug/ml hemoglobin intact cumulus cells to any other groups (P<0.05). On the other hand, when added to hemoglobin at 96 hrs, 1 ug/ml hemoglobin with cumulus cell group was significantly increased the percentage of developing into morulae and blastocysts to any other groups (P<0.05), and similar trend that of added at 48hrs. The overall means of the percentage of developing into morulae and blstocysts in 1ug/ml hemoglobin group was significantly increased than those of any other groups (P<0.05) and cumulus co-culture with hemoglobin was increased the in vitro developing rate of IVM/IVF embryos. In CR/sub 1aa/ medium treated with L-NAME 0, 10, 50 and 100mM, the developmental rate of morula plus blastocysts were 55.6%, 64.9%, 58.8% and 66.7%, respectively. The L-NAME did not affect the developmental rate and the cell numbers of blastocysts in all treated groups. These results indicate that hemoglobin and cumulus co-culture can increase the proportion of embryos that developed into morulae and blastocysts, but cell numbers of blastocysts were not affect in all groups.
Keywords
Bovine IVM/IVF embryos; Hemoglobin; L-NAME; CR/sub 1aa/; Nitric oxide;
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