• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1243-1252
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• 2019

The purpose of this study is to investigate the optimum conditions of biomass pretreatment with potassium hydroxide (KOH) for efficient utilization of cellulose, hemicellulose and lignin from Miscanthus. The optimization of variables was performed by response surface methodology (RSM). The variation ranges of the parameters for the RSM were potassium hydroxide 0.2~0.8 M, reaction temperature 110~190℃ and reaction time 10~90 min. The optimum conditions of alkali pretreatment from Miscanthus were determined as follows: concentration of KOH 0.47 M, reaction temperature 134℃ and reaction time 65 min. At the optimum conditions, the yield of cellulose from the solid fraction after pretreatment was predicted to be 95% by model prediction. Finally, 66.1 ± 1.1% of cellulose were obtained by verification experiment under the optimum conditions. The order contents of solid extraction were hemicellulose 26.4 ± 0.4%, lignin 3.7 ± 0.1% and ash 0.5 ± 0.04%. The yield of ethanol concentration of 96% was obtained using separated saccharification and fermentation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.4
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• pp.336-342
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• 1988

An experiment was carried out to know the effects of paclobutrazol application time on the lodging related traits and yield of a rice variety. Seomjinbyeo, at two nitrogen leveles. Paclobutrazol (3kg 10a of 0.6% G) was applied 43, 33, 23, and 13 days before heading (DBH) and lodging related traits were observed 10, 20, 30, and 40 days after heading (DAH). Earlier applications of paclobutrazol reduced the length of lower internodes and later applications reduced the upper internodes. Culm length tended to decrease as time of paclobutrazol application delayed. Although lodging was not occurred in all plots. lodging index of paclobutrazol treated plots was lower than that of control and increased with maturity, Fresh weight of shoot increased up to 30 DAH and then decreased. Both fresh weight and breaking strength did not show consistent tendency with time of paclobutrazol application and observation. Direct effect of shoot fresh weight contributing to lodging index decreased with maturity. but that of breaking strength and culm length was similar during the ripening stages. Direct effect contributing to lodging index was greater in the order of breaking strength culm length. and fresh weight 40 DAH. Starch content of culm base decreased up to 20 DAH and then increased up to 40 DAH. Cellulose content increased up to 20 DAH, but hemicellulose and lignin did not change significantly during the ripening stages. Starch, cellulose, hemicellulose, and lignin contents of culm base did not correlated with breaking strength and time of paclobutrazol application did not show consistent results. Paclobutrazol applied 13 DBH did not reduce yield of rice. but the earlier applications reduced yield due to a reduced number of spikelets per panicle.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1994.07a
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• pp.23-24
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• 1994

본 연구는 성숙과의 저장중에 세포벽분해효소가 세포벽을 분해해서 물성의 변화를 유발함으로 일어나는 과실의 연과가 품질과 저장성의 저하 뿐만 아니라 영양적, 경제적 손실을 초래한다는 점을 고려하여 대추의 성숙중에 일어나는 연화현상을 연구하고자 성숙에 따른 경도, 세포벽분해효소의 활성, 세포벽 다당류 pectin질, 비섬유성 중성당 및 조직의 변화를 조사하였다. 경도는 대추의 숙성에 따라 감소하였고, polygalacturonase와 $\beta$-galactosidase의 활성은 각각 변색기와 완숙기에 나타난 이후 급격히 증가하였다. 세포벽 다당류인 pectin질과 알칼리 지용성 hemicellulose는 완숙기가지 증가햐였으나 cellulose는 완숙기에 산가용성 hemicellulose와 cellulose를 제외한 세포벽 다당류의 함량은 다소 감소하였다. 대추의 세포벽 비섬유성 중성당으로 rhamnose, arabinose, xylose, mannose, galactose, glucose가 동정되었고, 성숙동안에 pectin질에서는 arabinose, mannose, galactose와 총 비섬유성 중성당의 함량이 감소하였고, 산가용성 hemicellulose에서는 xylose와 mannose가 뚜렸하게 증가하였으나 중성당은 변화없었으며, 알칼리 가용성 hemicellulose에서는 성숙에 따른 변화가 거의 없었다. pectin질의 경우 수용성 pectin, EDTA 용해성 pectin 및 총 pectin은 성숙중에 증가하는 경향이있으나 불용성 pectin은 감소하는 경향이였으며 과숙기에는 불용성 psctin EDTA용해성 pectin 및 총 pectin의 함량은 모두 현저히 감소하였다. 대추의 성숙중 조직에서는 pectin질로 구성된 중충의 붕괴현상이 뚜렸하게 나타났다.발이 절실히 필요한 실정이다. 이러한 배경으로 본 강연에서는 효소적갈변 저해제의 개발과 그들의 식품가공에의 적용 현환 및 화장품, 의약품으로의 응용에 대해 설명하고자 한다.L주에 비해 S주는 수정후 용과가 더 심하다. 9) 화분관의 행동은 수정력과 완전히 일치된다. 즉 L-selfing, $L{\times}L$, S-selfing, $S{\times}S$등의 부적법 수분에서는 화분관은 화주의 미중에서 정지되지만 $L{\times}S$, $S{\times}L$,에서는 수분 약 40-50분 후이면 화분관은 자방까지 도달된다. 10) S주는 웅본으로 오인되어 있지만 인위적법수분을 하면 수정력이나 화분관의 행동은 L주에서와 동일하다. 11) S화분은 완전하지만 L화분은 약 70%가 내용공허한 Adortive pollen 이다. 12) L화분중 나머지 30%도 S화분에 비해서 염색도가 낮은것이 많고 S화분 같이 농염되는 것은 극히 소수이다. 13) 본장물은 분화가 고도로 진행된 전형적인 이형예작물이여 마치 Dimorphism 에서 Dioecious 에로 이행되는 수가 있다는 것을 표시하는 증거가 되는 것 같다. 다소 높은 산소농도 3%~5% 이산화탄소 농도 5~8%에서 저장하는 것이 효과적일 것으로 판단되었다.철쭉군목으로 대표되나 군단이 하의 군목들은 다소 차이를 보이는 것으로 나타났다. 중간상인이론의 수정이 필요하다고 본다.가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([

• Food Science and Preservation
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• v.15 no.4
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• pp.532-541
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• 2008

A central composite design was applied to investigate the effects of the independent variables of NaOH concentration(X1) and extraction time(X2) on dependent variables such as Yield(Y1), Xyl/Ara ratio(Y2), uronic acid(Y3), $\beta$-glucan(Y4) and total sugars(Y5) of hemicelluloses separated from rice bran. The Coefficients of determination(R2) in various models ranged from 0.8626 to 0.9319. Yield increased with increased NaOH concentration and extraction time. The optimum extraction conditions were NaOH concentration at 2.45M and extraction time of 24.2 h. Predicted values at the optimized conditions were acceptable, compared with experimental values. The structural characteristics of an optimum hemicellulose extract were explored. As a result, it showed that the surfaces of hemicellulose had a highly irregular reticulated structure. And also it was both small and large molecular particle in the hemicelluloses. Their average molecular weights were in the ranges $235{\sim}240$ kDa and $8.0{\sim}9.4kDa$, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.16 no.3
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• pp.49-54
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• 1987

Procedures for Dietary Fiber(DF) determination were applied to some common vegetables. The samples selected in this study were: Radish(Leaf), Chinese cabbage, Spinach, Shepherd's purse, Red pepper(Leaf), Perilla(Leaf), Soybean(Leaf) and Cabbage. DF was analyzed by the method of detergent fractionation. Values for NDF, ADF, Lignin, Hemicellulose, Cellulose were obtained. NDF for most samples was $12.9{\sim}27.4%$, except Soybean(Leaf) (41.9%), ADP was $7.7{\sim}16.9%$. Lignin was around $1.0{\sim}2.1%$; Red pepper (Leaf) (7.6%), Perillar(Leaf) (5.7%), Soybean(Leaf) (4.2%) were exceptions. $Hemicellulose(NDF{\sim}ADF)$ was about $2.9{\sim}12.0%$, except Soybean(Leaf) (25.0%). $Cellulose(ADF{\sim}Lignin)$ was $6.3{\sim}13.0%$. This paper describes two properties of the fiber of commonly eaten vegetables; Water-Holding Capacity(WHC) and Density. The capacity of the ADP to hold water was estimated. The WHC measurements differed from 6.6g per g of ADP for Red pepper(Leaf) to 10.4g per g of ADP for Radish(Leaf). Radish(Leaf), Soybean(Leaf) had the greatest WHC whereas Red pepper(Leaf), Perillar(Leaf) had the least. Two types of density determinations are shown. Direct (non-packed) values largely correspond with bulk(packed) density.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.3
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• pp.176-179
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• 1985

Digestion trials were conducted to evaluate intake and digestibility of grass harvested at different dates. Silage was prepared from first-growth forage (predominantly timothy) which cutting dates; 4 June (vegetative, called early-cut) and 30 June (heading, called late-cut). The silage was fed to wethers at restricted and ad libitum levels of intake. The crude protein and crude fat of silage decreased with advancing maturity, while contents of fibre increased. DM intake of early-cut and late-cut silage were $99g/W_{kg}^{0.75}\;and\;75g/W_{kg}^{0.75}$, respectively. In digestibility and TDN, significant differences (p<0.01) between early-cut and late-cut silage were found. There was no significant difference in DM digestibility of early-cut silage between restricted and ad libitum feeding, but DM digestibility was decreased markedly in ad libitum feeding that out of account in fecal condition. Digestibility of all composition in early-cut silage, except for hemicellulose, was not observed with increasing levels of intake. Digestibility of crude protein, NDF and hemicellulose in late-cut silage were decreased (p<0.05) in ad libitum when compared with restricted feeding. Results of this study suggest that digestibility of early and late forages harvested as silage varies with levels of intake. Thus, relationship levels of intake and digestibility must be considered cautiously.

• Journal of the Korean Wood Science and Technology
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• v.30 no.4
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• pp.8-16
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• 2002

This study was performed to produce the high reactive lignin zero substrates from autohydrolyzed wood resources. In chemical compositions of used raw-materials, there were significant differences between two species, Japanese larch (Larix leptolepis) and oak (Quercus mongolica) woods. Japanese larch contained 25 to 3.5 times higher amounts of extractives than oak wood, which is mainly derived from high content of arabinogalactan in Japanese larch wood. Oak wood has 5% lower lignin content and 3% higher holocellulose and pentosans than larch wood. Concerned to changes in wood components during autohydrolysis pretreatment at 22 kg/cm² steaming pressure for 5~60 min, glucose content was constant during pretreatment, while hemicellulose and lignin were abruptly changed. Hemicellulose fraction was decreased significantly and lignin contents increased because of its condensation reaction with hemicellulose degradation products. The pH of hydrolyzates during pretreatment was decreased, reached upto pH 3 and since then leveled off. In the case of oak wood, same tendency was observed as in Japanese larch. Autohydrolysis followed by sodium chlorite and sulfite or bisulfite pretreatment was very effective in delignification of the substrates. In particular, two-stage delignification of autohydrolyzed woods with alkali and O₂-alkali resulted in very low lignin content substrates, such as 0~0.2% lignin substrate.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.247-247
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• 2005

• Journal of Ginseng Research
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• v.7 no.2
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• pp.169-171
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• 1983

The structural components of cell wall in various parts of Korean ginseng were analysed. Pectin was abundant in the cortex and epidermis of root, and leaf. Hemicellulose in root was more than in other parts of ginseng. Cellulose content of stem and seed coat was much higher compared to other parts and lignin content was highest in seed coat.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.3
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• pp.383-388
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• 1993

Three experiments were conducted to investigate the effect of cellulose addition on high (Exp. I) and low (Exp. II and III) dry matter barley straw silages. In Exp. I : 1 kg barley straw + 16 g glucose + 600 g water + 0 g as control (E0G), + 2 g (E2G), + 4 g (E4G), + 6 g (E6G), and + 8 g (E8G) of cellulose as treatments were ensiled. In Exp. II and III, 10 g glucose was and was not added, respectively, into 2 kg barley straw + 0 g (E8W, E8T) of cellulose as treatments. Samples were stored for 10 (Exp. I) and 7 (Exp. II and III) months at $21^{\circ}C$. The effect of cellulose addition on the fermentation and breakdown of the polysaccharides component in the silos at ensiling occurred more markedly at low dry matter silages rather than at the high ones. All cellulose treated silages were well preserved (pH below 5 in Exp. I and below 4 in Exp. II and III), while lactic acid and ethanol concentration increased. The fibrous fraction (ADF, NDF, crude fiber, hemicellulose, and cellulose) significantly (p<0.01) decreased (except hemicellulose content in Exp. I) compared with corresponding untreated silages. In vitro dry matter digestibility values (IVDMD) were similar for all silages. The present study showed that cellulose addition improved the potential nutritional and fermentation quality of barley straw silage.