한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제36권4호
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  • Pages.1243-1252
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  • 2019
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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고온 압출식 반응시스템을 이용한 억새 바이오매스의 KOH 전처리조건 최적화

Optimization of KOH pretreatment conditions from Miscanthus using high temperature and extrusion system

  • 차영록 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 박성민 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 문윤호 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 김광수 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 이지은 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 권다은 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 강용구 (농촌진흥청 국립식량과학원 바이오에너지작물연구소)
  • Cha, Young-Lok (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Park, Sung-Min (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Moon, Youn-Ho (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Kwang-Soo (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Lee, Ji-Eun (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Kwon, Da-Eun (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Kang, Yong-Gu (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA)
  • 투고 : 2019.12.16
  • 심사 : 2019.12.26
  • 발행 : 2019.12.30
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초록

억새와 같은 초본계 바이오매스로부터 cellulose, hemicellulose, lignin 등 주요성분을 추출하기 위해서는 알칼리 전처리가 효율적이며, 본 연구에서는 수산화칼륨(KOH)을 이용한 전처리 조건을 최적화하였다. 전처리 변수의 최적화는 반응표면분석법(RSM)을 적용하였다. RSM의 변수는 3개였으며, 변수범위는 각각 KOH 0.2~0.8M, 반응온도 110~190℃ 및 반응시간 10~90min 이었다. 억새의 알칼리 전처리를 위한 최적조건은 KOH 농도 0.47M, 반응온도 134℃ 및 반응시간 65min 이었다. 최적 전처리 조건에 따라 전처리를 수행한 후 고형물의 cellulose 함량은 66.1±1.1% 이었으며, hemicellulose 및 lignin 함량은 각각 26.4±0.4%, 3.7±0.1% 이었다. RMS 모델식에 따라 계산된 예측값은 실제값 대비 95% 범위 내에서 유효하였다. 최종적으로 전처리물을 동시당화발효를 통해 검증한 결과 에탄올 생산 수율은 96% 이었다.

The purpose of this study is to investigate the optimum conditions of biomass pretreatment with potassium hydroxide (KOH) for efficient utilization of cellulose, hemicellulose and lignin from Miscanthus. The optimization of variables was performed by response surface methodology (RSM). The variation ranges of the parameters for the RSM were potassium hydroxide 0.2~0.8 M, reaction temperature 110~190℃ and reaction time 10~90 min. The optimum conditions of alkali pretreatment from Miscanthus were determined as follows: concentration of KOH 0.47 M, reaction temperature 134℃ and reaction time 65 min. At the optimum conditions, the yield of cellulose from the solid fraction after pretreatment was predicted to be 95% by model prediction. Finally, 66.1 ± 1.1% of cellulose were obtained by verification experiment under the optimum conditions. The order contents of solid extraction were hemicellulose 26.4 ± 0.4%, lignin 3.7 ± 0.1% and ash 0.5 ± 0.04%. The yield of ethanol concentration of 96% was obtained using separated saccharification and fermentation.

키워드

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