• Korean Journal of Ecology and Environment
• /
• v.49 no.1
• /
• pp.22-30
• /
• 2016

Environmental changes exert harmful effects on organisms inhabiting coastal regions. These changes are also associated with reduced production in aquaculture farms. In this study, we investigated internal and external responses of two Bivalvia species (Crassostrea gigas and Mytilus galloprovincialis) in Gamak Bay under stressful environmental conditions in aquaculture farms. We investigated external responses such as weight, size, and environment exposure time, and analyzed the expression of the HSP70 gene. C. gigas HSP70 gene expression level was significantly high in the C3 aquaculture farm site, but the weight and size of C. gigas were high in the C2 aquaculture farm site. The response of C. gigas HSP70 mRNA was associated with the environmental exposure time in each aquaculture farm. Expression of M. galloprovincialis HSP70 gene was found to be significantly higher in the M2 aquaculture farm site than in the M1 site, whereas the weight of M. galloprovincialis was observed to be higher in the M1 site. The size and environmental exposure time of M. galloprovincialis were similar between M1 and M2 sites. In addition, HSP70 sequences of C. gigas and M. galloprovincialis showed high similarity with that of another marine species. According to our results, there were differences in internal responses following environmental stress in aquaculture farms, with respect to HSP70 gene expression. The results suggest that the HSP70 gene is a useful molecular indicator for monitoring stress responses in Bivalvia species in the field.

• Journal of Marine Life Science
• /
• v.7 no.1
• /
• pp.29-36
• /
• 2022

Sea cucumber, Aposticopus japonicus, is a major invertebrate species in the coastal regions of Korea. To evaluate the short-term stress levels according to the releasing methods, this study investigated the gene expression profiles of heat shock protein 90 (HSP90) by real-time quantitative polymerase chain reaction. When the juvenile sea cucumbers were packed in the vinyl bag with oxygen followed by transportation for 30 min or air-exposed for 1 h, the HSP90 gene expression levels in the experimental groups were significantly increased compared to those of the control groups (transported group, p=0.001; air-exposed group, p=0.032). The experimental group at 6 h post-release by seed-spreading method and at 2~6 h post-release by underwater hose-releasing method on board a fishing boat showed that the levels of HSP90 gene expression were not statistically significant but decreased slightly compared to the control group (seed-spreading group, p=0.069; hose-releasing group, p=0.093). On the other hand, the HSP90 gene expression showed an increasing pattern as the time passed (~6 h) after underwater release of juvenile sea cucumbers by divers (p=0.061). These results suggest that HSP90 gene expression can be used to investigate short-term stress response and effective releasing methods of juvenile sea cucumbers.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.52 no.2
• /
• pp.21-38
• /
• 2024

This study aims to establish an efficient street green area to improve the urban thermal environment and enhance pedestrian thermal comfort. Specifically, This study identified parameters applicable to green space planning and design, analyzed thermal environment mitigation mechanisms for each parameter, and, based on these findings, proposed methods for tree species selection and planting in green space planning and design. To achieve this, 61 papers were selected through a four-stage process from both domestic and foreign sources. The selected papers were analyzed, and the following main results were derived: In open street canyons with high stress levels due to low aspect ratios and high sky view factors(SVF), broadleaf trees with wide crown widths, low trunk heights, high leaf area index(LAI), and high crown heights were found effective in reducing heat, thereby increasing the amount and quality of shade. In contrast, in deep and narrow street canyons with relatively low heat stress due to high aspect ratios and low SVF, broad-leaved trees with narrow crown widths, high trunks, low crown heights, and low LAI were effective in reducing heat by enhancing ventilation. This study can serve as fundamental data for establishing standards for street green spaces to improve the thermal environment of street canyons and enhance thermal comfort of pedestrians. Additionally, it can be valuable when selecting the location and prioritizing street green spaces. Moreover, it is anticipated to be a foundational resource for creating guidelines for green space planning and design in response to climate change.

• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.5
• /
• pp.749-756
• /
• 2014

The present study was carried out to investigate the effects of dietary antioxidants on pro-inflammatory cytokines, heat shock protein (HSP) and antioxidant status in broiler chicks under summer conditions. A total of 162, 3-d-old broiler chicks were randomly assigned to a basal diet (CON) and the basal diet supplemented with vitamin C (200 mg/kg diet, VCD) or vitamin E (100 mg/kg, VED) until 35 day of age. All birds were exposed to summer diurnal heat stress at average daily fluctuations of temperature between $32^{\circ}C$ to $34^{\circ}C$ at day to $27^{\circ}C$ to $29^{\circ}C$ at night for the entire feeding periods. There was no significant difference in body weight, feed to gain ratio and the relative organ weight except the thymus in response to dietary vitamin C or E supplementation. However, the mRNA expression of interleukin (IL)-$1{\beta}$, IL-6, interferon (IFN)-${\gamma}$, Toll like receptor (TLR)-4 and HSP70 in the liver of birds fed diet containing vitamin C significantly (p<0.05) decreased compared with those in birds fed basal diet. Dietary vitamin E also showed a significant (p<0.05) decrease in the mRNA expression of IL-6 and HSP70 compared with a basal diet. Total antioxidant status (TAS) in serum of birds fed vitamin C supplemented diet was significantly (p<0.05) higher with than that in birds a basal diet. Lipid peroxidation in serum and liver resulted in a significant (p<0.05) decrease in response to dietary vitamin C or E supplementation. In conclusion, dietary supplementation with antioxidant vitamins, especially vitamin C resulted in a significant decrease in the mRNA expression of pro-inflammatory cytokines and HSP70, and higher antioxidant parameters than that of birds on the basal diet under summer conditions.

• Journal of Life Science
• /
• v.21 no.5
• /
• pp.664-670
• /
• 2011

Oxidative stress results in sustained release of heat shock protein 90 (HSP90) from vascular smooth muscle cells (VSMCs). We investigated whether extracellular HSP90 predisposed VSMCs to pro-inflammatory phenotype. Exposure of human aortic smooth muscle cells to HSP90 not only significantly enhanced CXCL10 secretion but also increased CXCL10 transcription. HSP90-mediated CXCL10 secretion was attenuated by OxPAPC, a TLR-2/4 inhibitor, and curcumin, a TLR-4 dimerization inhibitor. Inhibitors of diphenyleneiodium chloride and the Akt pathway also attenuated CXCL10 secretion in response to HSP90. The gene delivery of I${\kappa}$B using recombinant adenoviruses and treatment with resveratrol, which inhibit NF-${\kappa}$B activity, significantly attenuated HSP90-induced CXCL10 secretion from VSMCs. We propose that extracellular HSP90 contributes to an inflammatory reaction in the stressed vasculature by inducing CXCL10 expression of VSMCs, and that TLR-4, Akt, and NF-${\kappa}$B play active roles in the process.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.35 no.10
• /
• pp.1161-1170
• /
• 2011

This paper presents a solution to the inverse problem for the service boundary conditions of thermal-flow and structure analysis in a catalyst substrate. The exhaust-gas purification efficiency of a catalyst substrate is influenced by the shape parameter, catalyst ingredients and so on and is estimated by the thermal flow uniformity. The formulations of the inverse problem of obtaining the thermal-flow parameters (inlet temperature, velocity, heat of reaction, convective heat-transfer coefficient) and the direct problem of estimating from a given outlet temperature distribution are described. An experiment was designed and the response-surface optimization technique was used to solve the proposed inverse problem. The temperature distribution of the catalyst substrate was obtained by thermal-flow analysis for the predicted thermal-flow parameters. The thermal stress and durability assessments for the catalyst substrate were performed on the basis of this temperature distribution. The efficiency and accuracy of the inverse approach have been demonstrated through the achievement of good agreement between the thermal-flow response surface model and the results of experimental vehicle tests.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.15 no.1
• /
• pp.75-78
• /
• 2007

To define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause activated eggs were compared with differential expression using cDNA array. Among those clones, mRNA transcript from hsp20.8A, which was expressed at a high level in diapausing eggs that had been incubated at $25^{\circ}C$ for 30 days after oviposition, whereas, in the eggs exposed to $15^{\circ}C$ for 30 days, $5^{\circ}C$ for 60 days, the expression of mRNA decreased. On the other hand, the expression of mRNA during embryogenesis observed abundantly at 4 to 6 days after heat-HCl treatment and later at 9 to 10 days after just before hatching. This result was suggested for us that hsp20.8A was expressed in response to embryogenesis as well as physical stress.

• Proceedings of the KIEE Conference
• /
• 2009.07a
• /
• pp.2219_2220
• /
• 2009

The characteristics of the Superconducting Magnetic Energy Storage (SMES) system are faster response, longer life time, more economical, and environment friendly than other Uninterruptible Power Supply (UPS) using battery. Fast charge and discharge time of SMES system can provide powerful performance of improving power quality in the grid. In order to demonstrate the effectiveness of SMES, the authors make a 10kJ SMES system for connection with RTDS (Real Time Digital Simulator). Because the characteristics of superconducting magnet are very important in SMES system, the necessary items such as thermal characteristic, mechanical stress and protection circuit should be considered. In this paper, the authors experimented thermal characteristics of the 10kJ SMES system. The experiment was accomplished using a simulation coils made of aluminium. It has same dimension of the 10kJ class HTS SMES coil. The coil was cooled with GM (Gifford -McMahon) cryocooler through the OFHC (Oxgen Free High thermal Conductivity) conduction bar. The test results of cool down and heat loads characteristics of the simulation coils are described in detail.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.28 no.2
• /
• pp.127-135
• /
• 2006

Objectives: The extent of bone formation that occurs at the interface of metallic implants and bone is determined by the number and activity of osteoblastic cells. Stress proteins may be contributing determinants of cell viability in altered environments. Hsp27 is a small Mr hsp which is known as a molecular chaperone. Methods: To better understand how heat shock protein 27 contributes to endosseous implant - associated metal ions affects on osteoblastic cell viability, the effect of chromium and titanium ions were compared to effects of cadmium ions in the ROS17/2.8 osteoblastic cell line. Results: ROS17/2.8 osteoblastic cell line demonstrated ion - specific reductions in growth; reductions were significantly greater for cadmium than for chromium or titanium. Chromium impaired growth of cultures without altering cell viability measured using the MTT assay. A stable transformed cell line expressing additional hsp27(clone "A7") was resistant to the toxic effects of titanium and partially protected from cadmium toxicity. Conclusions: A role for hsp27 in protection of osteoblastic cells from metal ion toxicity is supported by the chromium - induced elevations in hsp27 abundance and the behavior of the A7 cell line in response to metal ions in culture. Similar biochemical responses to altered cellular environments may contribute to the fate of tissues adjacent to select metallic implants.

• KSBB Journal
• /
• v.16 no.6
• /
• pp.626-631
• /
• 2001

Lactobacilli have been considered to play important roles in the health of human vagina. They secrete inhibitory substances to prevent vaginal infection by pathogenic organisms. In a previous study, we have isolated several lactobacilli from Korean woman and one of them (KLB46) was selected and indentified as Lactobacillu crispatus which showed high antimicrobial activity. In this study. cold adaptation prior to subsequent stresses exposure was examined whether L. crispatus KLB46 maintain the viability better than the non-adapted calls under stresses. For pharmaceutical formulation, the lyophilization process is required where stresses such as freezing/thawing and dehydration are routinely applied. Formulated L. crispatus KLB46 can be used for ecological treatment of bacterial vaginosis. The response of cold-adapted cells to other environmental stresses such as acid, heat, ethanol, NaCl, and H$_2$O$_2$ was also examined. The results showed that cold-adapted cells maintained higher survival rate compared with the non-adapted cells (freezing-thawing. 3-folds; dehydration: 3-folds; acid, 3-folds; heat, 10-folds). However, we did net observe any positive effect of cold adaptation on other stresses such as ethanol, NaCl and H$_2$O$_2$. When chloramphenicol was added during cold adaptation, adaptation effect was abolished. This confirms that de novo protein synthesis is necessary during the adaptation process. Moreover, we have identified cold shock protein homolog that codes for a major cold shock protein by PCR amplification using degenerate primers.