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Molecular Characterization of Small Heat Shock Protein(hsp20.8A) from the Silkworm, Bombyx mori  

Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Go, Hyun-Jeong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Goo, Tae-Won (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Seong, Su-Il (Department of Life Science, College of Natural Science, University of Suwon)
Yun, Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim, Seong-Ryul (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Park, Kwan-Ho (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim, Ik-Soo (College of Agriculture & Life science, Chonnam National University)
Jeon, Jae-Pil (Korea National Institute of Health)
Kang, Seok-Woo (Department of Life Science, College of Natural Science, University of Suwon)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.15, no.1, 2007 , pp. 75-78 More about this Journal
Abstract
To define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause activated eggs were compared with differential expression using cDNA array. Among those clones, mRNA transcript from hsp20.8A, which was expressed at a high level in diapausing eggs that had been incubated at $25^{\circ}C$ for 30 days after oviposition, whereas, in the eggs exposed to $15^{\circ}C$ for 30 days, $5^{\circ}C$ for 60 days, the expression of mRNA decreased. On the other hand, the expression of mRNA during embryogenesis observed abundantly at 4 to 6 days after heat-HCl treatment and later at 9 to 10 days after just before hatching. This result was suggested for us that hsp20.8A was expressed in response to embryogenesis as well as physical stress.
Keywords
cDNA array; Hsp 20.8A; Embryogenesis; Bombyx mori;
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