Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

HSP27 MODULATION OF IMPLANT- ASSOCIATED METAL ION CYTOTOXICITY OF OSTEOBLASTIC CELLS

임프란트에 관련된 금속이온의 조골세포에 대한 세포독성에 미치는 Hsp27의 영향에 대한 실험적 연구

  • Yoon, Jung-Ho (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Ha, Dong-Jin (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Rim, Jae-Suk (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Kwon, Jong-Jin (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Jang, Hyon-Seok (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Lee, Eui-Seok (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Kim, Dae-Sung (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University)
  • 윤정호 (고려대학교 의과대학 치과 구강악안면외과학교실) ;
  • 하동진 (고려대학교 의과대학 치과 구강악안면외과학교실) ;
  • 임재석 (고려대학교 의과대학 치과 구강악안면외과학교실) ;
  • 권종진 (고려대학교 의과대학 치과 구강악안면외과학교실) ;
  • 장현석 (고려대학교 의과대학 치과 구강악안면외과학교실) ;
  • 이의석 (고려대학교 의과대학 치과 구강악안면외과학교실) ;
  • 김대성 (고려대학교 의과대학 치과 구강악안면외과학교실)
  • Published : 2006.03.31
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Abstract

Objectives: The extent of bone formation that occurs at the interface of metallic implants and bone is determined by the number and activity of osteoblastic cells. Stress proteins may be contributing determinants of cell viability in altered environments. Hsp27 is a small Mr hsp which is known as a molecular chaperone. Methods: To better understand how heat shock protein 27 contributes to endosseous implant - associated metal ions affects on osteoblastic cell viability, the effect of chromium and titanium ions were compared to effects of cadmium ions in the ROS17/2.8 osteoblastic cell line. Results: ROS17/2.8 osteoblastic cell line demonstrated ion - specific reductions in growth; reductions were significantly greater for cadmium than for chromium or titanium. Chromium impaired growth of cultures without altering cell viability measured using the MTT assay. A stable transformed cell line expressing additional hsp27(clone "A7") was resistant to the toxic effects of titanium and partially protected from cadmium toxicity. Conclusions: A role for hsp27 in protection of osteoblastic cells from metal ion toxicity is supported by the chromium - induced elevations in hsp27 abundance and the behavior of the A7 cell line in response to metal ions in culture. Similar biochemical responses to altered cellular environments may contribute to the fate of tissues adjacent to select metallic implants.

Keywords

References

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