• Journal of the Korean Wood Science and Technology
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• 제36권1호
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• pp.30-35
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• 2008

동해안 지역에서 산불로 고사된 소나무의 5년에 걸친 경시적 재질 변화를 역학성능을 중심으로 조사하였다. 고사에 따른 재질열화로 가장 크게 영향 받는 역학성능은 충격 휨 흡수에너지였다. 따라서 고사 초기의 재질변화를 예측하는 지표로 활용이 가능할 것으로 밝혀졌다. 고사 경과에 따라 변재의 재질열화가 심재에 비해 두드러지게 진행되었다. 열화의 정도는 충격 휨 흡수에너지가 가장 컸으며 압축강도가 가장 작았다. 고사 이후의 외관 변화와 역학성능의 열화진행 정도로 볼 때 산불피해 소나무의 유효한 활용을 위한 적정 벌채 시기는 산불발생 1년 이내가 바람직한 것으로 밝혀졌다.

• Journal of the Korean Wood Science and Technology
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• 제36권6호
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• pp.130-137
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• 2008

본 연구에서는 느티나무의 에탄올 추출액에서 실리카겔 칼럼크로마토그래피법을 이용하여 15개의 탄소로 이루어진 나프탈렌 구조의 세스퀴테르펜인 카달렌 화화물을 단리하였다. 단리한 카달렌 화합물은 HPLC, EI-MS와 $^1H$, $^{13}C-NMR$로 화학 구조를 규명하였다. 느티나무에서 단리한 카달렌 화합물의 분자량은 244 (m/z)으로 측정되었으며, 나프탈렌 구조의 1번 위치에 메틸기($CH_3$), 4번 위치에 isopropyl기가 부착되어 있으며, 7번 위치에 수산기(OH)와 3번 위치에 메톡실기($OCH_3$)가 치환된 7-hydroxy-3-methoxycadalene 구조를 이루고 있었다. 수율은 기건상태 느티나무 중량의 약 0.03% 정도로 나타났다. 카달렌은 느티나무의 잎과 수피에서는 전혀 발견되지 않았고, 목부 부위를 연륜별로 나누어 분석한 결과에 의하면 7-hydroxy-3-methoxycadalene은 변재부위에는 전혀 존재하지 않고 심재부에서만 발견되었다.

• 생약학회지
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• 제41권1호
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• pp.21-25
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• 2010

The MeOH extract of Rhus verniciflua heartwood inhibited lipid accumulation in 3T3-L1 adipocytes. Chromatographic methods including of silica gel, RP-18 and high-pressure liquid chromatography isolated sulfuretin and fisetin from the extract as active constituents. The isolated compounds, especially sulfuretin, strongly inhibited lipid accumulation in adipocytes. The treatment of sulfuretin and fisetin led to decreased expression of peroxisome proliferator-activated receptor-gamma ($PPAR{\gamma}$), as an important transcription factor in fat cell differentiation, which was equal to the decrease in the quercetin positive control. The presence of a hydroxyl group (C-5) in quercetin compared to fisetin, and the presence of C-2 double bonds in fisetin compared with fustin increased the inhibitory effect of lipid accumulation.

• Journal of the Korean Wood Science and Technology
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• 제35권2호
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• pp.29-38
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• 2007

This study was undertaken to investigate the variation of equilibrium moisture content (EMC) in transverse direction and three different directional (longitudinal, radial, and tangential) linear movements, and diffusion coefficients within a tree disc of Korean red pine (pinus densiflora). The EMC gradually increased in heartwood from pith. Therefore, the chemical components might differ even in heartwood and the radial variation in EMC might have a close relationship with the cellulose content within a cross section. The specific gravity increases gradually from pith and the porosity has not direct influence on the variation of EMC within a tree disk. Both the radial and tangential diffusion coefficients exhibited clear trend of increase from pith. The EMC change (${\Delta}EMC$) and tangential diffusion coefficient were close to be axisymmetrical but others were deviated from axisymmetry. The diffusion coefficient decreases with decreasing an activation energy and specific gravity, The diffusion coefficient increased with increasing ${\Delta}EMC$ and hygroscopicity of wood might be inversely proportional to the activation energy, The fJEMC may depend on the chemical constituents of cellulose, hemicellulose and lignin. As the number of sorption sites and sorption capacity of wood increase, therefore, it might be assumed that the hygroscopicity of wood increases while activation energy decreases. Modeling physico-mechanical behavior of wood, the variations should be considered to improve the accuracy.

• 치위생과학회지
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• 제7권1호
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• pp.31-35
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• 2007

Caesalpinia sappan L. (Leguminosae) is an oriental medicinal herb distributed in China and Taiwan, and its heartwood has been traditionally used as an analgesic, a therapy for thrombosis or tumor. This study was to investigate the proliferation and inhibition effects of Caesalpinia sappan extracts against human epithelial cell and cancer cell lines. The methanol extract of dried C. sappan heartwood was evaporated (KS-6), and then sequentially extracted by hexane (KS-01), chloroform (KS-02), ethyl acetate (KS-03), n-butanol (KS-04), and water (KS-05). After 48 hr of exposure, these fractions at a concentration of $50{\mu}g/ml$ significantly increased, and reduced cell proliferation in both human normal epithelial and cancer cell lines. The ethyl acetate fraction (KS-03) among organic solvent fractions was 120.2% of the most proliferation activity ($50{\mu}g/ml$) against HaCaT human epithelial cell. However, fractions from chloroform, butanolic and methanolic extract had 7.2, 28.7 and 20.8% of antiproliferative effect on HaCaT cell, respectively. In cell proliferation effects of C. sappan extract on HeLa, SiHa and C33A human cervical cancer cells, chloroform fraction (KS-2) was the most antiproliferative activity, its antiproliferative rate (dosage, $50{\mu}g/ml$) relative to control was 25.8, 12.2 and 17.4% for SiHa, HeLa and C33A, respectively. The results indicated that the six extract fractions could induce cell cycle stimulate or arrest in some way. Finally, further investigation is needed to assess the molecular mechanisms mediated anticancer activities of this plant.

• Natural Product Sciences
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• 제18권1호
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• pp.47-51
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• 2012

Among 38 Vietnamese medicinal plant extracts investigated for their ${\alpha}$-glucosidase inhibitory activity, 35 extracts showed $IC_{50}$ values below $250{\mu}g/mL$. The MeOH extracts of the heartwood of Oroxylum indicum, the seeds of Caesalpinia sappan, and the fruits of Xanthium strumarium exhibited strong ${\alpha}$-glucosidase inhibitory activity with $IC_{50}$ values less than $50{\mu}g/mL$. Fractionation of the MeOH extract of the heartwood of O. indicum led to the isolation of oroxylin A (1), oroxyloside (2), hispidulin (3), apigenin (4), ficusal (5), balanophonin (6), 2- (1-hydroxymethylethyl)-4H,9H-naphtho[2,3-b]furan-4,9-dione (7), salicylic acid (8), p-hydroxybenzoic acid (9), protocatechuic acid (10), isovanillin (11), and ${\beta}$-hydroxypropiovanillon (12). Compounds 1 - 3, 5, 6, 8, 10, and 12 showed more potent activities, with $IC_{50}$ values ranging from 2.13 to $133.51{\mu}M$, than a positive control acabose ($IC_{50}$, $241.85{\mu}M$). The kinetic study indicated that oroxyloside (2) displayed mixed-type inhibition with inhibition constant (Ki) was $3.56{\mu}M$.

• Applied Biological Chemistry
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• 제43권1호
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• pp.67-71
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• 2000

주요 목재해충인 흰개미에 대하여 천연산 편백이 가지는 살충활성의 크기를 정량적으로 취급하고, 수종간 및 서로 다른 함침재료 간에 발생되는 활성의 차이를 termiticidal activity value(TAV, 살충활성치)로 정의하여 해석했다. 그 결과, 흰개미에 대한 TAV는 편백>육박나무>대만편백>후피향나무의 순이었으며, 특히 편백의 활성은 다른 3 수종보다 $5{\sim}9$배 강력했다. 또 편백의 median survival dosage(MSD, 반수치사량)는 108.8 mg이었다. 편백에 의한 목분시험과 여과지 함침시험 어느 경우에도 흰개미 살충활성은 첨가 시료중량의 대수치와 그 median survival time(MST, 반수치사일수)의 대수치와의 사이에 반비례 관계가 성립했다. 또한 목분과 여과지에 대한 함침재료간 활성차는 메탄올추출물에서 작은 반면 중성부에서 3.21배로 매우 컸다. 그러나 중성부의 살충력은 원목분의 $17{\sim}53%$로서 실험 조작과정에서 $47{\sim}83%$의 활성손실이 확인되었다.

• Journal of the Korean Wood Science and Technology
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• 제36권4호
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• pp.84-92
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• 2008

강원도 양양 지역 산불 피해 소나무 목재의 피해 정도에 따른 해부 및 물리적 특성을 조사하였다. 피해 정도에 따라 수피의 탄화 깊이가 깊어지는 경향이 관찰되었으나 목질부까지 탄화가 진행되지는 않았으며 수지의 삼출정도에 차이를 보였다. 산불 피해목 변재의 생재함수율은 건전재보다 다소 낮게 나타났으나 심재의 경우에는 건전재보다 피해목이 다소 높게 나타났다. 생재밀도는 피해재 심재가 건전재 심재보다 다소 높게 나타났으며, 변재에서는 차이를 보이지 않았다. 형성층대를 관찰한 결과, 산불 피해를 강하게 입은 경우 형성층의 활동이 일어나지 않았고, 비교적 피해를 약하게 입은 경우에는 형성층의 활동이 관찰되었으나 세포분열 속도는 건전재보다 느렸다. 산불 피해를 강하게 입은 목재에서는 에피델리움세포와 방사조직의 세포벽이 짙게 염색되고 세포내에 과립형태의 물질이 관찰되었으며, 최외각 연륜 부위의 결정화도는 산불 피해재가 건전재보다 다소 높게 나타났다.

• 한국약용작물학회지
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• 제26권3호
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• pp.233-239
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• 2018

Background: Inflammation plays an important role in various diseases, including ulcerative colitis, Behcet's disease, and rheumatoid arthritis. In this study, we investigated the anti-inflammatory effects of Morus alba L. extracts obtained using different extraction methods (water extraction or high temperature extraction) on RAW264.7 cells. Methods and Results: Extracts from the central part (including the heartwood, sapwood, cambiun, and phloem) and bark (including the periderm and cortex) of Morus alba L. were obtained using either water or high temperature extraction. The following extract were obtained: MA1, water extract from the central part of Morus alba L., MA2, high temperature extract from the central part of Morus alba L., MA3, water extract from the bark of Morus alba L., and MA4, high temperature extract from the bark of Morus alba L. None of these extracts was observed to be cytotoxic to RAW264.7 cells. The MA2 extract reduced the production of LPS-induced NO (nitric oxide), $PGE_2$ (prostaglandin $E_2$), $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$ production in LPS-stimulated RAW264.7 cells. Conclusions: These results indicated that the inflammatory response was moderated by MA2. Treatment with MA2 could be used as a natural medicine for treating diseases involving inflammation. However, further experiments are required to determine how the high temperature extraction method alters the active ingredients in the extract and influences the anti-inflammatory effects of Morus alba L..

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1591-1596
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• 2006

Acaricidal effects of materials derived from Caesalpinia sappan heartwoods against Dermatophagoides farinae and D. pteronyssinus were assessed and compared with those evidenced by commercial benzyl benzoate and DEET. The observed responses varied according to dosage and mite species. The $LD_{50}$ values of the methanol extracts derived from C. sappan heartwoods were 6.13 and $5.44{\mu}g/cm^3$ against D. farinae and D. pteronyssinus, respectively. Furthermore, the ethyl acetate fraction derived from the methanol extract was approximately 8.71 more toxic than DEET against D. farinae, and 4.73 times more toxic against D. pteronyssinus. The biologically active constituent from the ethyl acetate fraction of C. sappan heartwood extract was purified via silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by $GC-MS,\;^1H-NMR,\;^{13}C-NMR,\;^1H-^{13}C\;COSY-NMR$, and DEPT-NMR spectroscopy, and identified as juglone (5-hydroxy-l,4-naphthoquinone). Based on the $LD_{50}$ values of juglone and its derivatives, the most toxic compound against D. farinae was juglone ($0.076{\mu}g/cm^3$), followed by benzyl benzoate ($9.143{\mu}g/cm^3$) and 2methyl-l,4-naphthoquinone ($40.0{\mu}g/cm^3$). These results indicate that the acaricidal activity of C. sappan heartwoods is likely to be the result of the effects of juglone. Additionally, juglone treatment was shown to effect a change in the color of the cuticles of house dust mites, from colorless-transparent to dark brownish-black. Accordingly, as a naturally occurring acaricidal agent, C. sappan heartwood-derived juglone should prove to be quite 'useful as a potential control agent, lead compound, and house dust mite indicator.