• BMB Reports
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• 제53권10호
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• pp.539-544
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• 2020

Skin aging appears to be the result of overlapping intrinsic (including genetic and hormonal factors) and extrinsic (external environment including chronic light exposure, chemicals, and toxins) processes. These factors cause decreases in the synthesis of collagen type I and elastin in fibroblasts and increases in the melanin in melanocytes. Collagen Type I is the most abundant type of collagen and is a major structural protein in human body tissues. In previous studies, many products containing collagen derived from land and marine animals as well as other sources have been used for a wide range of purposes in cosmetics and food. However, to our knowledge, the effects of human collagen-derived peptides on improvements in skin condition have not been investigated. Here we isolate and identify the domain of a human COL1A2-derived protein which promotes fibroblast cell proliferation and collagen type I synthesis. This human COL 1A2-derived peptide enhances wound healing and elastin production. Finally, the human collagen alpha-2 type I-derived peptide (SMM) ameliorates collagen type I synthesis, cell proliferation, cell migration, and elastin synthesis, supporting a significant anti-wrinkle effect. Collectively, these results demonstrate that human collagen alpha-2 type I-derived peptides is practically accessible in both cosmetics and food, with the goal of improving skin condition.

• 셀메드
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• 제10권2호
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• pp.11.1-11.14
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• 2020

Nigella sativa commonly known as Black seed, Black cumin or Kalonji (Family Ranunculaceae) is a widely used for its miraculous healing power. Use of N. sativa seeds and oil has splendid historical past in diverse traditional systems of medicine and food. In Tibb-e-Nabwi (Prophetic Medicine), it is considered as one of the greatest forms of healing medicine. Phytochemically; it contains fixed oil, protein, alkaloids saponin and essential oil. Therapeutic properties of this plant are due to the presence of thymoquinone which is one of major active component and has different beneficial properties. In Unani System of Medicine the diseases are treated with nontoxic herbal drugs. As per Unani classical literature N. sativa perform various pharmacological actions like carminative, anti-inflammatory, analgesic, diuretic, emmenagogue, galactagogue, expectorant etc. Ample of phytochemical, pharmacological and clinical researches has been executed on N. sativa., which may include antidiabetic, anticancer, immunomodulator, analgesic, antimicrobial, anti-inflammatory, bronchodilator, hepato-protective, renal protective, gastro-protective, antioxidant properties, etc. This review is an effort to summarize the literature on scientific researches of pharmacognostical characteristics, chemical composition and pharmacological activities of the kalonji seeds

• 한국환경생태학회지
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• 제26권3호
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• pp.463-471
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• 2012

본 연구는 산림기반형 한방치유 관광상품에 대한 수요자 욕구와 선호를 파악하여 향후 보다 표적화되고 차별화된 산림치유 관련 정책 수립과 한방치유 관련 프로그램의 개발을 위한 기초자료 제공을 목적으로 실시되었다. 서울에 거주하는 30대 이상 성인남녀 400명을 조사대상으로 성과 연령별, 거주지역별로 비례할당 표본추출법을 사용, 인터넷조사(CAWI)를 실시하여 총 317부가 최종 분석에 이용되었다. 산림기반형 한방치유 관광상품에 대한 이미지는 '산림욕, 숲속 걷기, 나무'가 다수(61.5%)를 차지했고, 선호 의견과 이용 의향은 각각 72.9%와 67.5%로 긍정적인 경향이 높았다. 단지 조성에 적합한 지역으로는 수도권이 53.6%, 강원도가 38.8%로 나타났다. 이용목적은 '스트레스 해소와 재충전', '산림휴양활동', '건강 유지 및 체력 증진'의 순이었다. 선호 치유요법은 '트래킹, 산책 등 걷기요법'이 가장 많았으며 '피트니스, 기체조 등 운동요법'이 그 뒤를 이었다. 상품선택 시 주요 고려 요인은 '이용 비용의 적정성', '음식과 이용 식자재의 적절성', '의료진 및 직원들의 친절성' 등으로 나타났으나 이용편의성과 의료서비스의 질, 관광활동 관련 요인 등 전 요인에 걸쳐 높은 점수를 기록하여 상품에 대한 수요자의 높은 기대수준을 보여주고 있다. 또한 인구통계학적 세분시장 별로 이용관련 요인들에 대한 선호에 차이점을 보이고 있어 관련 상품의 기획과 운영 시 차별화 되고 세분화된 수요자 욕구를 반영하는 것이 필요할 것으로 보인다. 그러나 본 연구는 상이한 수요자 선호를 파악함에 있어 인구통계학적 세분 시장만을 규명함으로써 가장 기초적인 단계에 머무른 한계점을 지니고 있어 후속연구에서는 다차원적이고 복합적인 수요자 욕구에 대한 보다 정교화된 규명이 요망된다.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.26-35
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• 2017

The hydrolysis of proteins constitutes an invaluable tool, granting access to a variety of peptide fragments with potentially interesting biological properties. Therefore, a hemoglobin (Hb) hydrolysate of Crocodylus siamensis was generated by digestion under acidic conditions. The antibacterial and antioxidant activities of the Hb hydrolysate were assessed in comparison with intact Hb. A disc diffusion assay revealed that the Hb hydrolysate exhibited antibacterial activity against eight strains of gram-positive bacteria and showed a higher efficacy than intact Hb. Moreover, the antioxidant activity of intact Hb and its hydrolysate was evaluated using ABTS and DPPH radical scavenging assays. The Hb hydrolysate exhibited free radical scavenging rates of 6-32%, whereas intact Hb showed a slightly higher activity. In addition, non-toxicity to human erythrocytes was observed after treatment with quantities of Hb hydrolysate up to $10{\mu}g$. Moreover, active fragmented Hb (P3) was obtained after purifying the Hb hydrolysate by reversed-phase HPLC. Scanning electron microscopy demonstrated the induction of bacterial cell membrane abnormalities after exposure to P3. Antibacterial and antioxidant activities play crucial roles for supporting the wound healing activity. Consequently, an in vivo mice excisional skin wound healing assay was carried out to investigate the effects of intact Hb treatment on wound healing in more detail. The results clearly demonstrate that intact Hb is capable of promoting 75% wound closure within 6 days. These findings imply that intact Hb of C. siamensis and its acid hydrolysate may serve as valuable precursors for food supplementary products benefitting human health.

• 한국조리학회지
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• 제22권7호
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• pp.11-21
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• 2016

본 연구의 목적은 음식관광 체험유형이 상품구매의도에 어떠한 영향을 미치는지를 알아보고, 또한 상품구매의도가 방문만족에 어떠한 효과가 있는지를 검증을 하는데 있다. 이를 위하여 대구음식관광박람회를 방문한 관광객들을 대상으로 설문조사를 실시하고, SPSS v. 20.0 통계패키지 프로그램을 활용하여 분석하였다. 연구 결과, 첫째, 음식관광 체험유형 하위변수 중 미식추구형, 지식추구형, 힐링추구형은 상품구매의도에 대하여 유의한 정(+)의 영향관계가 있는 것으로 분석되었고, 관계추구형은 종속변수인 상품구매의도에 대하여 아무런 영향관계가 없는 것으로 분석되었다. 둘째, 상품구매의도는 방문만족에 대하여 유의한 정(+)의 영향관계가 있는 것으로 분석되었다. 이는 음식관광객들은 음식관광 체험을 하면서 미식이라는 기본적 부분과 지식과 힐링 부분을 신경쓰면서 음식 관광을 한다. 또한 음식관광객들은 관계추구형에 있어서는 긍정적인 반응을 나타내지 못했다. 이는 음식관광객들을 위해 지역을 방문 시 프로그램이나 제도 등을 갖추어진 프로그램으로 계속해서 개발하고 개선해서 재방문을 할 수 있도록 노력하여야 할 것으로 판단된다.

• 한국식품영양과학회지
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• 제43권3호
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• pp.374-380
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• 2014

본 연구에서는 야관문 추출물의 마우스 대식세포에 대한 항염증 활성과 창상유발 동물실험 모델을 통한 창상치유 효과를 조사하였다. RAW264.7 세포에서 야관문 추출물은 0.2 mg/mL 이하 농도에서 세포생존에 영향을 주지 않았으며, 염증반응이 활성화된 대식세포에 대해 농도 의존적으로 유의적인 NO 생성 감소를 나타내었다. 창상유발 동물실험 모델에서 야관문 추출물을 함유한 화장품 조성물의 창상치유효과에 대해 육안적으로 관찰한 결과, SCO군과 CCO군보다 야관문 추출물을 함유한 SSP군에서 약 20~30% 빠른 상처면적 감소 효과를 나타내었으며, 반흔 크기 역시 약 12% 작게 형성되었다. 또한 SSP군 조직의 외피와 진피 재생회복속도가 빨라진 것을 Masson's trichrome 염색을 통해 확인할 수 있었으며, VEGF 및 TGF-${\beta}1$ 유전자 발현이 SCO군과 비교 시 각각 감소 및 증가하였다. 이러한 결과는 야관문 추출물이 항염증 및 교원질 생성 유도를 통한 조직재생 활성에 기여하여 창상치유 속도를 가속화하고 반흔 면적을 감소시킬 수 있는 피부 창상치유와 관련한 코스메슈티컬 소재로써 산업적 활용이 가능함을 보여준다.

• Journal of Periodontal and Implant Science
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• 제27권3호
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• pp.443-457
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• 1997

The purpose of this study was to evaluate the effects of ethanolic extracts of Scutellaria Radix on the alveolar bone formation in the extract socket of rat. Thirty-six Sprague-dawley rats were used in this study. Mean body weight of rat was $130{\pm}5g$. Experimental animal were administered 0.4% ,${\beta}-aminopropionitril$(Sigma, USA) with the solid commercial food for 5 days. All the maxillary 1st molar of the rats were extracted by using of the tissue forcep under the general anesthesia with Pentobarbital sodium(Tokyo Chemical Co, Japan) injection into intraperitoneal space. All the extracted rats were divided into two group, experimental group which were feeded the solid food mixed ethanolic extracts of Scutellaria Radix, and control group which were feeded same food without reagent. At 1, 3, 5, 7, 9 and 14th days after tooth extraction, rats in both groups were serially sacrificed respectively. All the specimen were treated as usual method and prepared Hematoxylin-eosin stain for the light microscopic observation. The results were as follows : 1. Bone formation of extracted socket starts from the area on remained periodontal ligament than other area. 2. In the case of administration of the extracted Scutellaria Radix showed rapid healing process of connective tissue than non-administrated group. 3. In the case of administration of the extracted Scutellaria Radixshowed rapid osteogenesis than non-administrated With above results, it was concluded that ethanolic extracts of Scutellaria Radix may play a favorable role on the healing process of exatraction socket after extraction in rats. It was suggested that further study to evaluate the different concentration and administration method of ethanolic extracts of the Scutellaria Radix into same experimental model.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 B
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• pp.1187-1189
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• 2003

With development of Circuit technology, accuracy of dielectric or induction healing apparatus was developed. This study aims at realization of food insecticidal apparatus using dielectric heating. Equipment to measure gram's permittivity or vermin's permittivity and expectation for heating temperature by computer simulation is necessary for construction of the system. And the effect of extermination of vermin for food was confirmed by experiment using the system.

• 한국식생활문화학회지
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• 제14권3호
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• pp.225-231
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• 1999

Perception and knowledge on the diet among 651 women living in Inchon were surveyed by questionnaires in March through May, 1998. Respondents consisted of middle school girls(12.3%), high school girls(16.4%), and women in twenties(16.4%), thirties(18.9%), forties(15.7%) and fifties and over(20.3%). Ratio of high school graduates was the highest(37.0%) among respondents, and there was a tendency of less education with ages. Seventy-six percent of women practised the diet for the self-satisfaction of a nice figure, 20% for healing diseases, and 3.4% for attracting boy friends' or husbands' attention. The purpose of the diet was significantly different with ages(p<0.01). The diet methods which the respondents perceived effective and wanted to practise were exercise and food therapy. Average score of the knowledge on the diet was 8.27 out of 10, and significantly different with ages and education(p<0.05). Twenties showed the highest score and high school girls, thirties, middle school girls, forties and fifties and over were followed in decreasing order. Also the score was in decreasing order of graduates of college, high, elementary and middle school.

• Preventive Nutrition and Food Science
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• 제8권1호
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• pp.46-53
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• 2003

The current study investigated the effect of Korean safflower (Carthamus tinctorius L.) seed powder and its water and ethanol extracts on bone metabolism during recovery from rib-fracture induced by surgical operation in rats. 10-week-old male Sprague-Dawley rats weighing about 320 g were divided into 9 groups after arrival: 10d control (AIN 76 semi-purified diet), 10d safflower seed powder (10d SS-powder), 10d safflower seed ethanol extract (10d SS-EtOH), 10d safflower seed water extract (10d SS-$H_2O$), 20d control (AIN-76 semi-purified diet), 20d safflower heed powder (20d SS-powder), 20d safflower seed ethanol extract (20d SS-EtOH), 20d safflower seed water extract (20d SS-$H_2O$), and 20d sham-operation (20d sham), The dietary level for all the supplements was 5% based on the raw material weight. The rats were fed the experimental diets for 10 days before the rib fracture operation and for a further 10 or 20 days after the operation. A number 9 rib was fractured surgically and a sham-operation also performed. The rats were then sacrificed on the l0th or 20th day after the operation. The body weight initially decreased after the operation in all the rib-fractured groups, then gradually recovered. The concentrations of plasma osteocalcin were higher in the control group than in all the safflower-supplemented groups 10 and 20 days after the rib-fracture (p < 0.05). The bone-specific ALP (alkaline phosphatase) activity was significantly higher in the SS-EtOH group than in the other groups 20 days after the rib-fracture (p < 0.05). The level of urinary DPD (deoxypridinoline) was significantly higher in the SS-EtOH and SS-$H_2O$ groups than in the other groups 10 days after the rib-fracture. When comparing the PTH (parathyroid hormone) and calcitonin levels, the SS-$H_2O$ group exhibited the highest PTH level among the groups 10 and 20 days after the rib-fracture. Thus, it was concluded that the bone turnover during the fracture-healing period was more rapid in the rats supplemented with safflower seed powder or its fractions than in the control rats. Furthermore, the SS-$H_2O$ fraction was identified as the most effective in stimulating bone remodeling, as bone resorption and bone formation were both significantly increased during fracture healing when compared to the control group.