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http://dx.doi.org/10.3746/jkfn.2014.43.3.374

Wound Healing Effects of Lespedeza cuneata Extract  

Jung, Hee Kyoung (BioHealth Convergence Center, Daegu Technopark)
Kim, Kil-Soo (College of Veterinary Medicine, Kyungpook National University)
Jeong, Yoo Seok (BioHealth Convergence Center, Daegu Technopark)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.3, 2014 , pp. 374-380 More about this Journal
Abstract
In this study, the anti-inflammatory effects of Lespedeza cuneata extract on macrophages and wound-healing in wound-induced animal experiments were investigated. In an anti-inflammatory test, 0.1 mg/mL of Lespedeza cuneata extract did not affect growth of RAW 264.7 cells, and Lespedeza cuneata extract suppressed nitric oxide (NO) generation from inflammation-induced macrophages in a concentration-dependent manner. Wounds on the skin of rats were treated with vehicle containing Lespedeza cuneata extract (SSP), vehicle (SCO), and commercial ointment (CCO). The wound and scar sizes in the SSP group were significantly reduced in comparison to the SCO and CCO groups (P<0.05). The epidermis and dermis of the SSP group also recovered faster than the SCO group based on Masson's trichrome staining. The gene expression levels of vascular endothelial growth factor (VEGF) decreased and transforming growth factor-beta 1 (TGF-${\beta}1$) increased in wound tissue from the SSP group compared to that from the SCO group. These results show that Lespedeza cuneata extract accelerates wound-healing through anti-inflammatory activity and induction of collagen regeneration as well as reduces the scar area surrounding wounds. Accordingly, Lespedeza cuneata extract could be useful as a cosmeceutical in the cosmetic industry.
Keywords
Lespedeza cuneata; cosmeceuticals; anti-inflammation; wound healing; wound repair;
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Times Cited By KSCI : 15  (Citation Analysis)
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1 Park JM, Lee SP, Son ES. 2002. An analysis on the regulations, technology, and market of cosmeceuticals. Kor J Technol Innovat Soc 5: 293-306.
2 Lee HH. 2007. A prospect on development of functional foods in the cosmetics industry. Kor J Aesthet Cosmetol 5: 83-90.
3 Ding JL, Lim IJ, Lee HD, Cha WS. 2006. Analysis of minerals, amino acids and vitamin of Lespedeza cuneata. Korean J Biotechnol Bioeng 21: 414-417.   과학기술학회마을
4 Waldron DR, Zimmerman-Pope N. 2003. Superficial skin wounds. In Textbook of Small Animal Surgery. Elsevier Health Science, Philadelphia, PA, USA. p 259-273.
5 Kim SJ, Kim DW. 2007. Antioxidative activity of hot water and ethanol extracts of Lespedeza cuneta seeds. Korean J Food Preserv 14: 332-335.
6 Kim HJ, Kim KS, Kim DI. 2012. Inhibitory effects of Lespedeza cuneata ethanol extract on ultraviolet-induced photo aging. J Korean Soc Food Sci Nutr 41: 1540-1545.   과학기술학회마을   DOI   ScienceOn
7 Choi KM, Lee CW, Lee MY. 2008. Effect of ore minerals in the healing of full-thickness skin injury model of rat. J Environ Sci 17: 809-816.   과학기술학회마을   DOI
8 Lee AY. 2005. Allergic contact dermatitis due to sodium fusidate, an ingredient of fucidin ointment. Medical Postgraduates 5: 261-263.
9 Han DO, Kim GH, Choi YB, Shim IS, Lee HJ, Lee YG, Kim JH, Chang GT, Hahm DH. 2005. Healing effects of Astragali radix extracts on experimental open wounds in rats. Korean J Oriental Physiology & Pathology 19: 92-97.   과학기술학회마을
10 Park ST, Kim JW, Jeong SH, Seo YM. 2012. The effect of extract from several herbs grown naturally in namwon province wound treatment. J Korean Biol Nurs Sci 14: 122-128.   과학기술학회마을   DOI
11 Song JH. 2006. Simple evaluation of the wound healing activity of a crude extract of root bark of Ulmus parvifolia. J Wonkwang Medi Sci 20: 313-318.
12 Mohideen S, Ilavarasan R, Hemalatha S, Anitha N, Sasikala E. 2003. Wound healing and diuretic activities of Canthium parviflorum lam. Nat Prod Sci 9: 102-104.
13 Kim DH, Yi HS, Yun HJ, Cha CM, Park SD. 2010. Anti-inflammatory effect of methanol extract of Keum-Ryung-Ja-San in mouse macrophages. Kor J Herbology 25: 89-98.   과학기술학회마을
14 Kim HH, Park GH, Park KS, Lee JY, An BJ. 2010. Anti-oxidant and anti-inflammation activity of fractions from Aster glehni Fr. Schm. Kor J Microbiol Biotechnol 38: 434-441.   과학기술학회마을
15 Kim JM, Kim DJ, Kim TH, Kim HS, Choe M. 2010. Anti-oxidative and anti-inflammatory effect of water extract from Perillae semen in RBL-2H3 cells. Korean J Nutr 43: 367-373.   과학기술학회마을   DOI   ScienceOn
16 Saeavanan BC, Sreekumar C, Bansal GC, Ray D, Rao JR, Mishra AK. 2003. A rapid MTT colorimetric assay to assess the proliferative index of two Indian strains of Theileria annulata. Vet Parasitol 113: 211-216.   DOI   ScienceOn
17 Hayashi Y, Tsuji S, Tsuji M, Nishida T, Ishii S, Iilima H, Nakamura T, Eguchi H, Miyoshi E, Hayashi N, Kawano S. 2008. Topical implantation of mesenchymal stem cells has beneficial effects on healing of experimental colitis in rats. J Pharmacol Exp Ther 326: 523-531.   DOI   ScienceOn
18 Knighton DR, Hunt TK, Thakral KK, Goodcon WH. 1982. Role of platelets and fibrin in the healing sequence. Ann Surg 196: 379-387.   DOI   ScienceOn
19 Hunt TK, Knighton DR, Thakral KK, Goodson WH 3rd, Andrews WS. 1984. Studies on inflammation and wound healing: angiogenesis and collagen synthesis stimulated in vivo by resident and activated wound macrophages. Surgery 96: 48-54.
20 Min DH, Kim DK, Lim JP, Yang JH. 2005. Transdermal drug delivery & therapeutic effect of the preparations of Lithospermi radix and Gardeniae fructus extracts on the burn & wound healing. J Kor Pharm Sci 35: 255-263.   과학기술학회마을   DOI   ScienceOn
21 Numata A, Hokimoto K, Yamaguchi H. 1980. C-Glycosylflavones in Lespedeza cuneata. Chem Pharm Bull 23: 964-965.
22 Thippeswamy T, Mckay JS, Quinn JP, Morris R. 2006. Nitric oxide, a biological double-faced janus-is this good or bad. Histol Histopathol 21: 445-458.
23 MacMicking J, Xie QW, Nathan C. 1997. Nitric oxide and macrophage function. Annu Rev Immunol 15: 323-350.   DOI   ScienceOn
24 Yoon JH, Moon HB. 1994. The effect of macrophages on the wound healing delayed by immunosuppression. J Korean Surg Soc 46: 10-22.
25 Schaffer MR, Tantry U, Gross SS, Wasserkrug HL, Barbul A. 1996. Nitric oxide regulates wound healing. J Surg Res 63: 237-240.   DOI   ScienceOn
26 Han SM, Lee KG, Yeo JH, Kweon HY, Woo SO, Baek HJ, Park KK. 2006. The effects of Bombyx mori silk protein for the expression of epithelial growth factor in the wound healing process of the hairless mouse. Korean J Seric Sci 48: 56-60.   과학기술학회마을
27 Kim JS, Han SH, Jung BK, Park SJ, Kim HW, Chae H, Kwon YK, Kim BJ. 2010. Effects of Korean herbal bathing candidates on allergic contact or wound induced dermatitis mice model. Korean J Oriental Physiology & Pathology 24: 481-489.   과학기술학회마을
28 Martin P. 1997. Wound Healing-aiming for perfect skin regeneration. Science 276: 75-81.   DOI   ScienceOn
29 Diegelmann RF, Evans MC. 2004. Wound healing: An overview of acute, fibrotic and delayed healing. Front Biosci 9: 283-289.   DOI
30 Muller MJ, Hollyoak MA, Moaveni Z, Brown TLH, Herndon DN, Heggers JP. 2003. Retardation of wound healing by silver sulfadiazine is reversed by Aloe vera and nystatin. Burns 29: 834-836.   DOI   ScienceOn
31 Macri L, Silverstein D, Clark RAF. 2007. Growth factor binding to the pericellular matrix and its importance in tissue engineering. Adv Drug Delivery Rev 59: 1366-1381.   DOI
32 Lee HJ, Lim GN, Park MA, Park SN. 2011. Antibacterial and antioxidative activity of Lespedeza cuneata G. Don. Korean J Microbiol Biotechnol 39: 63-69.   과학기술학회마을
33 Kwon DJ, Kim JK, Ham YH, Bae YS. 2007. Flavone glycosides from the aerial parts of Lespedeza cuneata G. Don. J Korean Soc Appl Biol Chem 50: 344-347.   과학기술학회마을
34 Cornelissen LH. 2004. Which molecules of the initial phase of wound healing may be used as markers for early detection of skin damage. http://www.mate.tue.nl/mate/pdfs/4703.pdf.
35 Hong JH, Kim HS, Kim HR, Park MK, Yoon CH, Lee SH, Kim HY, Park SH. 2005. Elevated serum levels of vascular endothelial growth factor in Behcet's syndrome. J Korean Rheum Assoc 12: 189-196.
36 Crouvezier S, Powell B, Keir D, Yaqoob P. 2001. The effects of phenolic components of tea on the production of pro- and anti-inflammatory cytokines by human leukocytes in vitro. Cytokine 13: 280-286.   DOI   ScienceOn
37 Lee JH, Byun JA, Yeo SW, Kim JK, Park YS. 2011. Role of interleukin-10 on mouse otitis media model using lipopolysaccharide. Korean J Otorhinolaryngol-Head Neck Surg 43-47.
38 Eun JS. 2011. Effect of Lespedeza cuneata G. Don on the activity of murine immune cells. Korean J Oriental Physiology & Pathology 25: 837-842.   과학기술학회마을
39 Kapoor M, Howard R, Hall I, Appleton I. 2004. Effects of epicatechin gallate on wound healing and scar formation in a full thickness incisional wound healing model in rats. Am J Pathol 165: 299-307.   DOI   ScienceOn
40 Rolfe KJ, Richardson J, Vigor C, Irvine LM, Grobbelaar AO, Linge C. 2007. A role for TGF-${\beta}1$-induced cellular responses during wound healing of the non-scarring early human fetus. J Invest Dermatol 127: 2656-2667.   DOI   ScienceOn
41 Shah M, Revis D, Herrick S, Baillie R, Thorgenirson S, Ferguson M, Roberts A. 1999. Role of elevated plasma transforming growth factor-${\beta}1$ levels in wound healing. Am J Pathol 154: 1115-1124.   DOI   ScienceOn
42 Kader KN, Coyle CH. 2007. Reactive oxygen and nitrogen species: implications for cardiovascular device engineering. J Biomed Mater Res Part B 83B: 138-144.   DOI   ScienceOn