• Title/Summary/Keyword: harvesting system

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THE MOST APPROPRIATE ANTIMITOTIC TREATMENT OF ARA-C IN SCHWANN CELL-ENRICHED CULTURE FROM DORSAL ROOT GANGLIA OF NEW BORN RAT (신생 백서 척수후근절의 슈반세포 배양을 위한 Ara-C 분열억제제의 최적 효과에 대한 연구)

  • Kim, Soung-Min;Lee, Jong-Ho;Ahn, Kang-Min;Kim, Nam-Yeol;Sung, Mi-Ae;Hwang, Soon-Jeong;Kim, Ji-Hyuck;Jahng, Jeong-Won
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.30 no.2
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    • pp.100-107
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    • 2004
  • Schwann cell, one of important components of peripheral nervous system, interact with neurons to mutually support the growth and replication of embryonal nerves and to maintain the different functions of adult nerves. The Ara-C, known as an antimitotic agent, have been used to have high effectiveness in eliminating fibroblasts during Schwann cell culture period. This enrichment effect is also known to be cummulative with each successive pulse of Ara-C applied and is due to a progressive loss of fibroblasts. But the cytotoxicity by Ara-C is also cummulative and noticeable over the period. To determine the most effective application time and interval of Ara-C in the Schwann cell culture, we observed the Schwann cell purity and density with the Ara-C treatment in plain and three-dimensional culture from dorsal root ganglion of new born rat. By culturing dispersed dorsal root ganglia, we can repeatedly generate homogenous Schwann cells, and cellular morphology and cell count with mean percentages were evaluated in the plain culture dishes and in the immunostainings of S-100 and GFAP in the three-dimensional culture. The Ara-C treated cultures showed a higher Schwann cell percentage ($31.0%{\pm}8.09%$ in P4 group to $65.5%{\pm}24.08%$ in P2 group), compared with that obtained in the abscence of Ara-C ($17.6%{\pm}6.03%$) in the plain culture after 2 weeks. And in the three-dimensional culture, S-100 positive cells increased to $56.22%{\pm}0.67%$ and GFAP positive cells to $66.46%{\pm}1.83%$ in G2 group (p<0.05), higher yield than other groups with Ara-C application. Therefore, we concluded that the Ara-C treatment is effective for the proliferation of Schwann cells contrast to the fibroblasts in vitro culture, and the first application after 24 hours from cell harvesting and subsequent 2 pulse treatment (P2 group in plain culture and G2 group in three-dimensional culture) was more effective than other application protocols.

The Effect of Plant Coverage on the Constructed Wetlands Performance and Development and Management of Macrophyte Communities (식생피도가 인공습지의 질소 및 인 처리효율에 미치는 영향과 습지식물의 조성 및 관리)

  • Ham, Jong-Hwa;Kim, Hyung-Chul;Koo, Won-Seok;Shin, Hyun-Bhum;Yun, Chun-Gyeong
    • Korean Journal of Ecology and Environment
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    • v.38 no.3 s.113
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    • pp.393-402
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    • 2005
  • The field scale experiment was performed to examine the effect of plant coverage on the constructed wetland performance and recommend the optimum development and management of macrophyte communities. Four sets (each set of 0.88 ha) of wetland (0.8 ha) and pond (0.08 ha) systems were used. Water flowing into the Seokmoon estuarine reservoir from the Dangjin stream was pumped into wetland system. Water depth was maintained at 0.3 ${\sim}$ 0.5 m and hydraulic retention time was managed to about 2 ${\sim}$ 5 days; emergent plants were allowed to grow in the wetlands. After three growing seasons of the construction of wetlands, plant coverage was about 90%, even with no plantation, from bare soil surfaces at the initial stage. During the start up period of constructed wetlands, lower water levels should be maintained to avoid flooding newly plants, if wetland plants are to be started from germinating seeds. Effluent T-N concentration in low plant coverage wetland was higher in winter than high plant coverage wetland, whereas no T-P effluent concentration and removal efficiency difference was observed within 15% plant coverage. Dead vegetation affected nitrogen removal during winter because it is a source of organic carbon which is an essential parameter in denitrification. Biomass harvesting is not a realistic management option for most constructed wetland systems because it could only slightly increase the removal rate and provide a minor nitrogen removal pathway due to lack of organic carbon.

Application of GIS to the Universal Soil Loss Equation for Quantifying Rainfall Erosion in Forest Watersheds (산림유역의 토양유실량(土壤流失量) 예측을 위한 지리정보(地理情報)시스템의 범용토양유실식(汎用土壤流失式)(USLE)에의 적용)

  • Lee, Kyu Sung
    • Journal of Korean Society of Forest Science
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    • v.83 no.3
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    • pp.322-330
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    • 1994
  • The Universal Soil Loss Equation (USLE) has been widely used to predict long-term soil loss by incorporating several erosion factors, such as rainfall, soil, topography, and vegetation. This study is aimed to introduce the LISLE within geographic information system(GIS) environment. The Kwangneung Experimental Forest located in Kyongki Province was selected for the study area. Initially, twelve years of hourly rainfall records that were collected from 1982 to 1993 were processed to obtain the rainfall factor(R) value for the LISLE calculation. Soil survey map and topographic map of the study area were digitized and subsequent input values(K, L, S factors) were derived. The cover type and management factor (C) values were obtained from the classification of Landsat Thematic Mapper(CM) satellite imagery. All these input values were geographically registered over a common map coordinate with $25{\times}25m^2$ ground resolution. The USLE was calculated for every grid location by selecting necessary input values from the digital base maps. Once the LISLE was calculated, the resultant soil loss values(A) were represented by both numerical values and map format. Using GIS to run the LISLE, it is possible to pent out the exact locations where soil loss potential is high. In addition, this approach can be a very effective tool to monitor possible soil loss hazard under the situations of forest changes, such as conversion of forest lands to other uses, forest road construction, timber harvesting, and forest damages caused by fire, insect, and diseases.

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Reviewing Classification Scheme of Timber Assets for the National Accounting in Korea (국부통계 산출을 위한 입목자산 분류기준의 정립)

  • Chong, Se Kyung;Kim, Young-Hwan
    • Journal of Korean Society of Forest Science
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    • v.96 no.6
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    • pp.724-729
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    • 2007
  • Due to the low productivity in timber harvesting, economic values of forest resources have been rarely validated in Korea. Since the Korea Forest Service provided a statistic of the timber assets for the survey of the 4th National Accounting (1998), no more statistics or measurements have been provided. Further, it becomes an issue that there is no clear standard to classify timber assets into produced or non-produced assets. In this research, we intended to provide a reasonable standard that enables one to classify timber assets either in produced or non-produced assets. For this purpose, articles in the '1993 System of National Accounts (SNA)', which presented definitions and scopes of biological assets, were scanned. The articles related to timber assets in the Korean version of SNA were compared with those in the original version of SNA that were proposed by UN. The results show that the Korean version of SNA seems to have much emphasis on natural occurrence or plantation for the classification standard, while its original version rather focus on the existence of direct control, responsibility, and management by institutional units. According to the original version of SNA, naturally occurring assets, which were basically classified into non-produced assets, were treated as being cultivated and classified into the produced asset when they (or their growth) were under the direct control, responsibility, and management of institutional units. Therefore, a guidance was presented to renew the Korean version of SNA and to innovate the classification standards for timber assets.

Studies on the Behaviour of Radionuclides in the Soil-Plant System;1) On the Uptake of Cesium-137 by Soybean (토양(土壤)-식물계(植物界)에 대(對)한 방사성핵종(放射性核種)의 거동(擧動)에 관(關)한 연구(硏究);I. 대두작물(大豆作物)에 의(依)한 Cs-137의 흡수이행(吸收移行))

  • Ryu, Joon;Kim, Jae-Sung;Lee, Young-Il
    • Korean Journal of Environmental Agriculture
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    • v.2 no.1
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    • pp.30-34
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    • 1983
  • The present study was carried out to determine the effect of a radionuclide, cesium-137, in soybean, which is an element released usually from nuclear facilities. Soybean plants were grown on the pots treated with cesium-137 $0.5{\sim}60{\mu}Ci/1kg$ soil and the uptake, translocation and accumulation of the radiocesium in the plant parts were measured at different growth stage. The results are summarized as follows: 1) Visual toxic symptoms on the plants due to treatment of radioactive cesium were not observed up to $60{\mu}Ci/10Kg$ soil in a pot. 2) The uptake of cesium-137 in soybean plant was increased with increment of concentration applied, while the uptake of potassium was proportionally decreased, indicating to have an ion antagonistic relationship between them. 3) The absolute amounts of cesium-137 in the plants were gradually increased by the pod setting stage, but rather reduced at harvesting stage. The accumulation occurred more in the leaves and stems than the soybean seeds. 4) The rate of uptake was ranged from 0.069 to 0.005 with proportional decrease by increasing concentration applied and the rate of Cs-137 translocation from plants to seeds was averaged 38.6% in soybean plant. The concentration coefficient was 0.04 in the soybean seeds from the pots treated with $20{\mu}Ci$ of cesium-137 and decreased with increment of cesium-137 applied.

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Resistance to Airflow of Grain as Affected by Grain Moisture Content (곡물(穀物)의 함수율(含水率) 변화(變化)에 따른 송풍저항(送風抵抗)에 관(關)한 연구(硏究))

  • Kim, M.S.;Kim, S.R.
    • Journal of Biosystems Engineering
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    • v.11 no.2
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    • pp.55-65
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    • 1986
  • The resistance to the passage of airflow through various agricultural products is an important consideration in the design of an aeration or drying system. The amount of resistance to airflow varied widely from one kind of grain to another, and depended upon airflow rate, surface texture and shape of the particles, the size and configuration of voids, and foreign and fine material in the grain bed. The airflow rate was the major factor considered on this kind of study in the early stages. But these days, the studies on the resistance to airflow of grain affected by grain moisture content and foreign and fine material have been widely carried out. However the foreign an fine material in the grain bed could not be a major factor on the study in Korea because there were only a few grain process procedure after harvesting it. The objectives of this study were to investigate the effect of moisture content and airflow rate on airflow resistance to grain, and to develop a model to predict the static pressure drop across the grain bed as a function of moisture content and airflow rate. The rough rice varieties, Akibare, Milyang 15 (Japonica types), Samkwang, Backyang (Indica types)and covered barley variety, Olbori, which were harvested in 1985 were used in the experiment after cleaning them. Resistances to airflow of grain were investigated at four levels of moisture content (13-25%, wb.) for ten different airflow rates($0.01-0.15m^3/sm^2$). The results of this study are summarized as follows; 1. Theaverage bulk densities were $585.3kg/m^3$ for rough rice and $691.6kg/m^3$ for barley at the loose fill, and were $648.8kg/m^3$ for rough rice and $758.2kg/m^3$ for barley at the packed fill. The pressure drops at the packed fill beds were approximately 1.4 to 1.8 times higher than those at the loose fill beds. 2. The pressure drops across grain beds deceased with the increase of moisture content and increased with airflow rate. The decreasing rates of pressure drop across grain beds according to the moisture contents at the lower airflow rates were higher than those at the higher airflow rates, and the increasing rates of pressure drop according to the airflow rates at the lower moisture contents were higher those at higher moisture contents. 3. The pressure drop across barley bed were much higher than rough rice beds and the pressure drops across Japonica type rough rice beds were a little higher than Indica type. 4. The mathematical models to predict the pressure drop across grain beds as a function of moisture content and airflow rate were developed from these experiments.

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The Analysis of effectiveness of the rainfall harvesting system by using infiltration-storage in the catchment area (유역 침투-저류방식을 이용한 빗물이용시설의 효과 분석)

  • 최계운;정기일;윤용진;현지환
    • Proceedings of the Korea Water Resources Association Conference
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    • 2004.05b
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    • pp.1213-1218
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    • 2004
  • 인류의 발전을 주도하였던 수자원은 현대에 이르러 음용수, 공업용수, 농업용수 등의 사용목적을 가지고 활용되는 한편 하천, 호수 등 자연환경과 어우러져 인간의 삶을 윤택하게 해주는 기능을 동반하여 다양한 형태로 활용되기에 이르렀다. 그러나, 수요 증가에 따른 개발증가로 수자원 부존량을 점차적으로 저하시키고 있고, 산업의 발전에 부가되는 환경 오염 등의 영향으로 현재 활용이 가능한 수자원의 양은 점차 줄어들고 있는 추세이다. 직접유출량이 발생하는 포장 면적이 증가할수록 침투가 가능한 면적이 줄어들어, 강우시 발생하는 다량의 수자원을 활용하지 못하고 하천으로 방류하게 되므로 점차 수자원 부족 현상이 가시화되고 있는 현 시점에서는 적극적인 활용 대책의 마련이 반드시 필요한 실정이다. 이와 같은 이유로 미국, 일본, 유럽 등 선진국에서는 빗물을 받아 활용할 수 있는 빗물이용시설에 대한 연구와 실제 적용을 통해 수자원의 효율적 활용을 유도하고 있으면, 국내의 경우 1990년대 중반부터 유출저감시설 연구 및 시범지역 적용을 시작으로 현재 빗물이용시설을 포함하는 연구가 활발하게 진행되고 있다. 빗물이용시설은 활용 면적에 따라서 건물의 옥상면을 통하여 유출되는 빗물을 저류하여 활용하는 방식과, 일정 면적에 내리는 빗물을 침투-저류하여 활용하는 두 가지의 방식으로 크게 구분 할 수 있다. 본 연구에서는 두 가지 방법 중, 유역 침투-저류 방식을 이용한 빗물이용시설에서의 강우강도에 따른 유출량 및 저류량 변화를 분석하고 아울러 침투-저류 방식을 활용하는 경우, 수질 변화를 검토하는 실험을 통하여 유역 저류-침투방식을 활용한 빗물이용시설의 적용성 여부를 확인하고자 한다.적법의 불확실도 $5\%\~10\%$에 비교하여 $5\%$ 내외의 정확도 수준을 지니고 있어 정확도 향상을 기대할 수 있다. 경제적인 측면에서도 매년 측정사업에 소요되는 비용과 비교하였을 때 장기적으로는 경제적인 방법이 될 수 있다. 하천수 등의 상호 관계 분석을 통해 장기간의 유역 물순환체계 변화를 분석할 수 있었다.골풀과, 닭의장풀과가 각 1종씩으로, 조사지점( I )보다 좀 더 많은 종이 분포하는 것으로 조사되었다. 또한 어류는 조사지점( I )에서 3회에 걸쳐 총 396개체가 채집되어 3목 8과 21종이었다. 이 중 한국 고유종은 11종이었고, 외래 어종은 검정우럭과 2종이 조사되었으며, Zacco platypus(피라미), Zacco temmincki(갈겨니), Acheilongnathus koreanus(칼납자루), Odontobutis platycephala(동사리), Coreoleuciscus splendidus(쉬리) 순으로 분포하고 있었고, Acheilognathus signifer(묵납자루)는 댐 건설 전에는 많이 분포하였으나 현장조사에서 서식을 확인 할 수 언어 개체수의 큰 감소내지 멸종된 것으로 추정되었다.에서 동시에 시행하였다. 수술 후 1년 내 시행한 심초음파에서 모든 환아에서 단지 경등도 이하의 승모판 폐쇄 부전 소견을 보였다. 수술 후 조기 사망은 없었으며, 합병증으로는 유미흉이 한 명에서 있었다. 술 후 10개월째 허혈성 확장성 심근증이 호전되지 않아 Dor 술식을 시행한 후 사망한 예를 제외한 나머지 6명은 특이 증상 없이 정상 생활 중이다 결론: 좌관상동맥 페동맥이상 기시증은 드물기는 하나

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Quali-Quantitative Analysis of Flavonoids for Mulberry Leaf and Fruit of 'Suhyang'

  • Ju, Wan-Taek;Kwon, O-Chul;Lee, Min-Ki;Kim, Hyun-Bok;Sung, Gyoo-Byung;Kim, Yong-Soon
    • Korean Journal of Environmental Agriculture
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    • v.36 no.4
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    • pp.249-255
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    • 2017
  • BACKGROUND: Globally, mulberry (Morus sp.) is exploited for feeding leaf to silkworms in order to obtain silk fiber or for animal feedstock production. Also, mulberry fruit is known to a by-product that was produced from mulberry tree after harvesting leaves for silkworm rearing, as a yield and consumption of mulberry fruit was increased, it has been fixing to a newincome crop. Mulberry leaves and fruits are used for the health benefits of human beings. Mulberry contains various bioactive components, such as alkaloids and flavonoids. Mulberry flavonoids are an important part of the diet because of their effects on human nutrition. The flavonoids in mulberry leaf and fruit of 'Suhyang'(Morus alba L.) were determined. METHODS AND RESULTS: Flavonoids for mulberry leaf and fruit of 'Suhyang' were analysed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS)technique. An UPLC-DAD-QTOF/MS system was used, and identification of mulberry leaves constituents was carried out on the basis of the complementary information obtained from LC spectra, MS ions, and MS/MS fragments. The mulberry leaf (16 flavonoids) and fruit (9 flavonoids) were isolated and analyzed from Suhyang using UPLC-DAD-QTOF/MS chromatogram. To the best of our knowledge, Quercetin 3-O-(6"-O-malonyl) glucoside and quercetin 3-O-rutinoside (rutin) was detected on the highest content in leaf and fruit, respectively and further research will be devoted to evaluate their biological activity. CONCLUSION: Obtaining information about the concentration of functional materials in mulberry leaves could contribute to the development and promotion of processed, functional products and offer possible industrial use of 'Suhyang', holding promises to enhance the overall profitability of sericulture.

Effect of Cultural Environments and Nitrogen Fertilization Levels on the Anthocyanin Accumulation of Purple-fleshed Potato (Solanum tuberosum L.) Variety Jasim (재배환경 및 질소시비량이 자주색 감자 '자심'의 안토시아닌 색소 축적에 미치는 영향)

  • Jeong Jin-Ceol;Chang Dong-Chil;Yoon Young-Ho;Park Chun-Su;Kim Sung-Yeol
    • Journal of Bio-Environment Control
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    • v.15 no.2
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    • pp.204-210
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    • 2006
  • The purple color in flesh of 'Jasim' potato (Solanum tuberosum L.) tubers is due to an accumulation of anthocyanin. The experiment was conducted to determine what is the key factor among cultural environments that affect the accumulation of anthocyanin in 'Jasim' potato tubers, and the effect of nitrogen fertilization levels on that. Through analysis of anthocyanin content of purple-fleshed tubers produced under the different cultural environments of three localities and two planting dates, we determined that lower temperature during 30days before harvesting resulted in higher anthocyanin accumulation of 'Jasim' potato tubers. In the experiment of different level of nitrogen fertilization, 'Jasim' potato tubers produced in highland (Daegualryeong) showed higher content of anthocyanin in the treatment of recommended level than in treatment of lower or higher level as compared with the recommended level. However, significant difference of anthocyanin content among different nitrogen fertilization levels was not observed in lowland (Gangnung). These results suggest that the selection of locality or cropping system and the optimal fertilization management are very important factors for production of higher pigmented tubers in purple-fleshed potato.

The most appropriate antimitotic treatment of Ara-C in Schwann cell-enriched culture from dorsal root ganglia of new born rat

  • Kim, Soung-Min;Jahng, Jeong-Won;Lee, Jong-Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.32 no.1
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    • pp.42-51
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    • 2006
  • Schwann cell, one of important components of peripheral nervous system, interact with neurons to mutually support the growth and replication of embryonal nerves and to maintain the different functions of adult nerves. The Ara-C, known as an antimitotic agent, have been used to have high effectiveness in eliminating fibroblasts during Schwann cell culture period. This enrichment effect is also known to be cummulative with each successive pulse of Ara-C applied and is due to a progressive loss of fibroblasts. But the cytotoxicity by Ara-C is also cummulative and noticeable over the period. To determine the most effective application time and interval of Ara-C in the Schwann cell culture, we observed the Schwann cell purity and density with the Ara-C treatment in plain and three-dimensional culture from dorsal root ganglion of new born rat. By culturing dispersed dorsal root ganglia, we can repeatedly generate homogenous Schwann cells, and cellular morphology and cell count with mean percentages were evaluated in the plain culture dishes and in the immunostainings of S-100 and GFAP in the three-dimensional culture. The Ara-C treated cultures showed a higher Schwann cell percentage (31.0%${\pm}$8.09% in P4 group to 65.5%${\pm}$24.08% in P2 group), compared with that obtained in the abscence of Ara-C (17.6%${\pm}$6.03%) in the plain culture after 2 weeks. And in the three-dimensional culture, S-100 positive cells increased to 56.22%${\pm}$0.67% and GFAP positive cells to 66.46%${\pm}$1.83% in G2 group (p<0.05), higher yield than other groups with Ara-C application. Therefore, we concluded that the Ara-C treatment is effective for the proliferation of Schwann cells contrast to the fibroblasts in vitro culture, and the first application after 24 hours from cell harvesting and subsequent 2 pulse treatment (P2 group in plain culture and G2 group in three-dimensional culture) was more effective than other application protocols.