In Korea, shiitake mushroom, Lentinula edodes, is cultivated on artificial medium containing oak sawdust and wheat bran. The annual production of spent mushroom substrate (SMS) of shiitake, a byproduct of the mushroom industry, is estimated to reach over 50,000 tons per year. This study aimed to improve the use of SMS as a novel bioresource. Hot water extracts of SMS after the first and third harvest were prepared and their bioactivities evaluated. Hot water extracts of uninoculated medium and shiitake were used as controls. Extracts of SMS showed higher radical scavenging of DPPH anions, ABTS cations, nitrites, and a higher reducing power than those of shiitake or medium extracts. After the first and third harvests at 0.5 mg/disc, SMS extracts showed no antibacterial or antifungal activities against the pathogenic and food-spoilage bacteria and fungi. However, they showed good inhibitory activities against ${\alpha}$-glucosidase at 0.5 mg/ml. In addition, SMS extracts had strong anti-coagulation activities via their inhibition of thrombin, prothrombin, and blood coagulation factors without platelet aggregation activity. Our results suggested SMS should no longer be perceived as a useless byproduct but should be understood as a novel bioresource, the extracts of which could be developed as antioxidant, antidiabetic, and antithrombosis agents.
Background: Panax stipuleanatus represents a folk medicine for treatment of inflammation. However, lack of experimental data does not confirm its function. This article aims to investigate the analgesic and anti-inflammatory activities of triterpenoid saponins isolated from P. stipuleanatus. Methods: The chemical characterization of P. stipuleanatus allowed the identification and quantitation of two major compounds. Analgesic effects of triterpenoid saponins were evaluated in two models of thermal- and chemical-stimulated acute pain. Anti-inflammatory effects of triterpenoid saponins were also evaluated using four models of acetic acid-induced vascular permeability, xylene-induced ear edema, carrageenan-induced paw edema, and cotton pellet-induced granuloma in mice. Results: Two triterpenoid saponins of stipuleanosides R1 (SP-R1) and R2 (SP-R2) were isolated and identified from P. stipuleanatus. The results showed that SP-R1 and SP-R2 significantly increased the latency time to thermal pain in the hot plate test and reduced the writhing response in the acetic acid-induced writhing test. SP-R1 and SP-R2 caused a significant decrease in vascular permeability, ear edema, paw edema, and granuloma formation in inflammatory models. Further studies showed that the levels of inflammatory mediators, nitric oxide, malondialdehyde, tumor necrosis factor-α, and interleukin 6 in paw tissues were downregulated by SP-R1 and SP-R2. In addition, the rational harvest of three- to five-year-old P. stipuleanatus was preferable to obtain a higher level of triterpenoid saponins. SP-R2 showed the highest content in P. stipuleanatus, which had potential as a chemical marker for quality control of P. stipuleanatus. Conclusion: This study provides important basic information about utilization of P. stipuleanatus resources for production of active triterpenoid saponins.
Seo, Mi Hye;Cho, Young Sik;Yang, Chang Yeol;Yoon, Jung Beom
Korean journal of applied entomology
/
v.59
no.4
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pp.411-416
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2020
Whitefly populations found in heated tomato greenhouses were monitored across three locations in Korea (Nonsan and Buyeo, Chungnam Province; Iksan, Jeonbuk Province) from November to May during the 2015 -2018 growing seasons. From May to October, surveys were conducted in Jangsu (Jeonbuk Province), where retarding cultivation was performed. Whitefly populations were classified and quantified by location and year. The dominant species were Trialeurodes vaporariorum (Westwood) and Bemisia tabaci (Gennadius). The species populations were mixed, but the frequency of occurrece of B. tabaci was higher than that of T. vaporariorum. Although whitefly populations started to increase in late March, they sharply increased in May and the highest population density was observed at the end of the harvest season. Therefore, the management of greenhouse tomato whiteflies through monitoring should begin in early March. In the case of retarding cultivation, the management of whitefly populations should be implemented in June, before the density of whitefly populations increases.
Storage in the form of brown or milled rice saves space and cut cost rather than storage of rough rice, the common way of grain storage in Korea. But, brown or milled rice may be more susceptable to grain aging and sensory change than rough rice. Rice stored in government warehouse for 20 months after harvest was hulled into brown or milled rice and packaged with kraft paper bag (paper package), polyprophylen (PP package), and polyethylene (PE package). Then, after those rice packages were stored under room condition for one year, we investigated germinability, fat acidity, and sensory change to determine milling and packaging effects. Germinability of rough and brown rice was decreased significantly after long-term storge. In germination rate, Chucheong cultivar was lower than Milyang #23, brown rice was lower than rough rice, but there was no differences within packaging materials. TTC test which had a significant correlation with germinability can be used as a handy procedure for predicting grain germination. Fat acidity was increased as the order of rough < milled < brown rice in terms of milling, and PP < paper < PE package in terms of packaging materials. Especially, storage of brown rice increased fat acidity above 30 mg KOH, indicating one of characteristics of grain aging. To prevent from high fat acidity it was necessary to store in forms of rough grain with paper or PP packaging and milled rice with paper, or PP, or PE packaging. In sensory test of stored grain, eating quality in brown rice was the worst because of acidification of fatty acid. Also, eating quality of stored grain became worse as fat acidity increased.
Seed germination and dormancy of three green manure seeds such as hairy vetch, crimson clover and narrow-leaved vetch (Vicia angustifolia L.) produced in Milyang, southern part of Korea were investigated to provide a basic information on the stability of seedling establishment in green manure seeds. In addition, the effect of seed storage duration on the germinability of imported hairy vetch and crimson seeds was also investigated. To determine the seed maturity time of three green manure seeds, the seeds were harvested manually at May 25, May 30, and June 4, and the 100-seed weight, germinability and percentage of hard seed were evaluated. Freshly harvested seeds of three green manure crops were strongly dormant because of hard seed coat. Germination percentage of the three green manure crops varied depending on the seed harvest time ranging from 30 - 52% in hairy vetch, 16 - 35% in crimson clover and 2 - 61% in V. angustifolia, respectively. Of the three crops, crimson clover and V. angustifolia matured early on May 30 to June 4 (around 35 to 40 days after flower) but hairy vetch seed did not mature until June 4 based on the seed germinability. The matured seed of crimson clover and V. angustifolia germinated less than 20% while scarification on seed coat significantly overcome the innate dormancy. On the other hand, the seed germination of hairy vetch and crimson clover was lower under dark than in the presence of light. The germination percentage of the imported hairy vetch and crimson clover seeds showed high with 77 - 79%, 94 - 95%, respectively, but the seed germination significantly declined by 21 - 32%, 30 - 40% after one-year of storage under natural conditions. The seeds germinated only 8 - 13% for hairy vetch but crimson clover did not germinate at all after two years storage. These results indicate V. angustifolia and crimson clover mature at May 30 to June 4 but hairy vetch did not. The imported hairy vetch and crimson clover seeds should be used within one-year after import for stable seedling establishment in green manure-rice cultivation cropping system.
Lee Seong-Tae;Shon Gil-Man;Kang Jin-ho;Lee Yong-Ho
Korean Journal of Plant Resources
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v.18
no.1
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pp.1-7
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2005
This study was conducted to investigate the possibility of cultivating tea plants planted in the mountain area of west Gyeongnam province, Korea for 2 years from March 27th, 2001 to July 30th 2003. Ninety each plants collected from 5 different sife were cultivated in nursery cup pot$(\phi\;16cm)$. in the greenhouse condition and transplanted in 5 different location and monitored their survival growing state etc. The results obtained are as follow : 1. Survival rate tea plants after transfer to soil was relatively high Gaya-myeon Hanyang-gun with $90.0\%$ and the lowest in Buksang-meyon Geochang-gun with $80.0\%$. The tea plant collected from Gaya-myeon showed the best growing activity at early stage. 2. For the second year of harvesting time, survival rate was the highest in Machun-myeon Hamyang-gun with $92.2\%$ and the lowest was Buksang-myeon Geochang-gun with $76.7\%$. 3. For the 3rd year of harvesting time, it was impossible to data collection because of the most upper parts of plants were killed by severe freezing weather condition. In the Baekjeon-myeon, Hamyang-gun Buksang-myeon, Ungyang-myeon Geochang-gun, which are severly cold$(below\;-10^{\circ}C)$ in winter season, seems not a suitable places for tea plant cultivation since it is very different to harvest the young loaves in growing season. In conclnsion we could select two sites Gaya-myeon, Hapcheon-gun, Machun-myeon, Hamyang-gun, as tea plant cultivation in the mountain area of west-Gyeongnam province, korea.
Journal of the Korean association of regional geographers
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v.4
no.2
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pp.31-47
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1998
This paper deals with the historical geography of land-use and agriculture along the Lower Nam-River floodplains. The reclamation process of the river floodplains, the cultivation methods on the reclaimed lands, and the land-use patterns and processes are investigated. The Nam River, one of the major tributaries of the Nakdong River, flows through the boundary between Ham-An and Eu-Ryong Guns. Larger floodplains are located in Ham-An Gun. The floodplains of Ham-An Gun have been surveyed intensively in this study. In South Korea, the alluvial plains, mostly located along the river valleys, have been reclaimed to provide fertile agricultural lands. Those along the upper river valleys were reclaimed before those along the lower river valleys. The flood-plains of Han-An Gun were reclaimed to be the largest agricultural lands of the Gun. The natural levees along the Lower Nam-River Valley were identified before the reclamation processes but now hardly identified. Relatively larger floodplains are located along the tributary streams of the Nam River. Often there are low-lying back swamps between the natural levees and the hills/mountains that rise above the floodplains. The back swamps, called 'natural bog lands' in this region, have been reduced in size and in number through reclamation for the purpose of agricultural and industrial land-uses. Now about ten 'natural bog lands' are found in the Ham-An floodplains, and some of them are being reclaimed for the industrial land-use. This study suggests the emergent need of conservation for the remaining 'natural bog lands' in terms of ecology. Seven agricultural fields of large size, originated from the Nam-River floodplains, are identified in this study: Kun(큰들), Chung-Am(정암들), Chang-chi(장지들), Baek-San(백산들), Ha-Ki(하기들), Gu-Hae(구혜들), and Chang-Po(장포들) fields. The Kun field was reclaimed during the Japanese control and the Gu-Hae, in the 1950s. All of those except the above two fields were reclaimed after the mid-1960s. The Nam-River Dam in Chinju, completed in 1969, contributed the reclamation processes along the Lower Nam-River floodplains. The rice acreage of the region has been reduced slowly since 1970 but the rice production of the region has been relatively stable (Table 4). Rice culture had been the most important agriculture on the reclaimed lands for decades before the greenhouse vegetable cultivation became more important in the 1980s. Among the vegetables cultivated in the greenhouse, the watermelon is the dominantly leading one. Watermelons are usually harvested two or three times in a year though it is possible to harvest four times in one year. The rotation of watermelons and rice is common in the region. It is known the physical conditions of the Nam-River floodplains in Ham-An Gun is the most suitable for watermelon cultivation in South Korea.
The Ise Grand Shrine(伊勢神宮) dedicated to the sun goddess Amaterasu (天照大神), located in the city of Ise(伊勢市), Mie Prefecture of Japan, is a center of Japanese Shinto Shrines and composed of a large number of Shinto shrines centered on two main shrines, Naiku(內宮=皇大神宮) and Geku(外宮= 豊受大神宮). Historically it has kept very close relationship with Emperor, because its enshrined deity Amaterasu is generally said to be the ancestor of Imperial Family. The food and alcohol offering to the gods in Japanese Shinto rites are called Shinsen(神饌, ambrosia). Main subjects of this essay dealing with Shinsen are the various matsuri (rites) of Ise Grand Shrine and Emperor, such as Higoto-asayu-omike-sai(日別朝夕大御饌祭, offering repasts to the gods in the moring and evening everyday), Kan-name-sai(神嘗祭, offering of the year's new rice harvest), Shikinen-sengu-sai(式年遷宮祭, year of the ceremony), Nii-name-sai(新嘗祭, Ceremonial offering by the Emperor of newly-harvested rice to the gods), and Daijo-sai(大嘗祭, first ceremonial offering of rice by newly-enthroned Emperor). Then, the purpose of this essay is to examine not only the social, religious, and political but also cultural meaning of Shinsen especially in relation to Korea, basically introducing some types and characteristics of Shinsen with its mythological background and historical development. In so doing, I will show the concrete list of items and processes of Shinsen in those rites. For example, the social meaning of Shinsen might be examined in association with agricultural features, ancient dietary life, Japanese food, and its contemporary context etc. Besides, its religious meaning can be mentioned especially from the perspective of divine nature, life and rebirth etc. On the other hand, the politics was in ancient Japan originally called Matsurigoto which means the ancestral rites for gods. This suggests the political meaning of Shinsen that the politics in Japan has originated from Shinsen.
Journal of The Korean Society of Grassland and Forage Science
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v.5
no.1
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pp.22-32
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1985
Optimum pasture management during the summer season is an important factor to maintain good regrowth and persistence of pasture in Korea. This experiment was carried out to investigate the effects of the cutting management on the dead plant, weed appearance, regrowth and carbohydrate reserves in stubble, and dry matter yield of tall fescue dominated pasture during the mid summer season. For the test, a split plot design with 4 replications was treated with 2 different the third cutting times (July 12 and Aug. 4) as the mainplots, and 3 different cutting heights (3, 6 and 9 cm) at the third cut as the subplots, and the experiment was done at the experimental field of the Livestock Experiment Station, in Suweon, 1984. The results obtained are summarized as follows: 1. Considering the meteorological conditions during the experimental period, the temperature was a little higher by $2^{\circ}C$ than that of average year, especially the first and second decade of August were high. And the precipitation of 1984 tended to be low when compared with the average year. 2. Temperature of soil surface and underground tended to increase by $1-3^{\circ}C$ as the stubble height was low during the summer season. 3. Regrowth leaf length and leaf area after the third cut increased significantly with the high cutting height at the third cut. 4. A significant higher total nonstructural carbohydrate (TNC) content in stubble after the third cut was observed in the high stubble cut on July 12. The results indicate that the high stubble height reserves more carbohydrates for early regrowth stage after the third cut when compared with the low stubble. On Aug. 4, however, the recovery of TNC contents after the third cut was not effective due to high temperature and rainfall. 5. The percentage of dead plant after the third cut was found to be high with the low cutting height during the mid summer season (p<0.05). 6. With the low stubble height on July 12 cut, it was appeared that the percentage of weed was significantly increased (p<0.05), and main weeds appeared after the third cut were Echinochloa crusgalli>Digitaria sanguinalis>Cyperus iria>Rumex crispus, and so on. In case of cut on Aug. 4, weed appearance was no difference at three cutting heights. 7. Dry matter yield at the third cut was increased in the plot of cutting on Aug. 4 and stubble height (p<0.05). However, yields at the fourth and fifth cut were increased with high stubble height (p<0.05), regardless of harvest time. 8. In total dry matter yield after the third cut, there was no significant difference between the cutting time and forage yield. However, total yield on July 12 was increased with the high stubble height (p<0.05). 9. From the above results, it is suggested that the 9 cm cutting height during the mid summer season is the most effective for good regrowth, weed control and forage yield of tall fescue dominated pasture.
1. Objectives and Importance. Many silkworms have been damaged by nuclear polyhedrosis virus diseases thoughout the country every year causing a decease in cocoon production by approximately ten per cent per year. The damage caused by the infections virus has occured in spite of complete disinfection. In this respect, it is well known it is impossible, at the present time, to protect the silkworm from these virus infections through chemical and physical control methods. Therefore, this author has attempted to solve this urgent problem from the view point of heredity and breeding, discovering the different resistances and heritabilities among 120 stains collected from throughout the country, and selecting the ones with highest resistance for the basic materials in the silkworm breeding. 2. Results of work 1) The strains with strong resistance to the nuclear polyhedrosis virus diseases are N$_4$, N$\sub$15/, N$\sub$48/, C$\sub$55/ and Em. the log ED$\sub$50/ values of them vary between 0.799 and 1.611. The susceptible strains are N$\sub$20/, C$\sub$62/, N$\sub$76/, N$\sub$79/ and C$\sub$108/, the log ED$\sub$50/ values of them vary between 5.159 and 7.258. (Reference Table 4) The Japanese strain with a log ED$\sub$50/, value of 3.770 is the strongest, followed by the Chinese strain with a log ED$\sub$50/ value of 3.564. The weakest is the European strain with a log ED$\sub$50/ value of 3.3381. The direction coefficient of the regression equation of the susceptibility varies between 0.1 and 0.6, the uniformness of the resistance of the preserved strains of this country is comparatively low. The hereditary henomena of the resistance of each strain and the conerete method of its application for silkworm breeding main the subjects for later studies. 2) The content of water and ash in silkworm has not been correlated with the capability for resistance to the virus diseases(Reference. Table 8). but it is very significantly correlated with mortality rate (in common reaning). In the case of the silkworms which have just completed the fourth moulting the content of water and ash is not related to the mortality rate. In the case of the silkworms which have just completed the third moulting, however, the water( +0.326) and ash (+0.362) registered a high significance. The ash content in the first ($\div$0.520) and second ($\div$0.386) moults is highly significant but water content in both cases is not significant (Reference Table 7). 3) The No. 205 strain proved to be the best in character among the various F$_1$ hybrids. No. 204 was very good in strength but a little lower in cocoon character than the control. No. 212 was a little low in coccon character and mortality was average, but the cocoon harvest was the best among all the varieties offered for (Reference Table 9). 4), In short, the above mentioned strains which are known to have strong resistance to the virus disease are expected to provide basic data for breeding strong varieties. It is proposed that continued research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics.research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics.
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