• Molecules and Cells
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• v.19 no.2
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• pp.219-222
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• 2005

The a-subunit of Escherichia coli tryptophan synthase (${\alpha}TS$), a component of the tryptophan synthase ${\alpha}_2{\beta}_2$ complex, is a monomeric 268-residues protein (Mr = 28,600). ${\alpha}TS$ by itself catalyzes the cleavage of indole-3-glycerol phosphate to glyceraldehyde-3-phosphate and indole, which is converted to tryptophan in tryptophan biosynthesis. Wild-type and P28L/Y173F double mutant ${\alpha}$-subunits were overexpressed in E. coli and crystallized at 298 K by the hanging-drop vapor-diffusion method. X-ray diffraction data were collected to $2.5{\AA}$ resolution from the wild-type crystals and to $1.8{\AA}$ from the crystals of the double mutant, since the latter produced better quality diffraction data. The wild-type crystals belonged to the monoclinic space group C2 ($a=155.64{\AA}$, $b=44.54{\AA}$, $c=71.53{\AA}$ and ${\beta}=96.39^{\circ}$) and the P28L/Y173F crystals to the monoclinic space group $P2_1$ ($a=71.09{\AA}$, b=52.70, $c=71.52{\AA}$ and ${\beta}=91.49^{\circ}$). The asymmetric unit of both structures contained two molecules of ${\alpha}TS$. Crystal volume per protein mass ($V_m$) and solvent content were $2.15{\AA}^3\;Da^{-1}$ and 42.95% for the wild-type and $2.34{\AA}^3\;Da^{-1}$ and 47.52% for the double mutant.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.13 no.1
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• pp.23-26
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• 1980

This study was attempted to establish the basic data for evaluating the processing suitability of the mussel, Mytilus edulis, cultured by hanging method in Yeosu Bay, Korea. Seasonal variations in condition index and chemical .composition of the mussel were carried out from March 1978 to February 1979. The results are as follows: 1. The condition index was highest from December to April next year. 2. The glycogen content of mussel was 2.7 to $3.9\%$ from December to May next year and revealed the maximum value, $3.9\%$ in December and March. F and G-values as the condition indices of the mussel showed also the highest value from December to March. 3. The protein content of mussel was highest from July to August. 4. By the results of condition index and chemical composition of the mussel, the suitable harvest season as raw materials for processing is determined to be from December to April next year and from July to August.

• Journal of Korea Multimedia Society
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• v.11 no.3
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• pp.281-291
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• 2008

In this paper, we propose a new double talk detection algorithm which detects near end signals with less degradation, tracking echo path variation of echo canceler simultaneously. Our method makes use of a cross-correlation between channel input signals and estimated error signals and a normalized cross-correlation between microphone input signals and estimated error signals. By combing thresholds for these cross-correlations pertinently, this algorithm discriminates between variation of echo path and occurrence of double talk. These two cross-correlation are used to detect double talk periods, tracking echo path variation. During the detection period, adjustive adaptive filter is ceased to prevent the echo canceler from being disturbed by near end signals. Also, the echo canceler will still be kept on for tracking any variation in echo path. Through computer simulation results, it was confirmed that the proposed algorithm shows better performance, tracking echo path variation and detecting the double talk periods, than the Ye et. al's and the NLMS algorithms from ERLE viewpoint.

• Journal of Fashion Business
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• v.20 no.1
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• pp.142-156
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• 2016

Convergence of IT devices and fashion is enabling the industry to take fully different approaches in various areas including product planning, fabric selecting, distributing, and marketing. At the same time, it also transforms the definition of clothing itself. Convergence of IT technologies in the realm of fashion and textiles industries can create a powerful synergy through connection digital devices, such as mobile phone. In this context, this study attempts to suggest how IT technology can be efficiently harnessed through the usage of mobile devices in the planning stage of fashion materials, where the initial production plan of a clothing item is mapped out. This study ultimately aims to enhance the effectiveness of databases on fashion material information by using mobile devices to utilize NFC, an RFID technology having as much revolutionary power as Internet - which can be the convergence between IT and fashion across the software dimensions. To pursue this research, data on fashion material information regarding 200 woven fabric specimens were provided by textile companies. The information includes elements such as its composition, weight, width, yarn, density and sales report. These pieces of information were organized into a database. Drawing on this data, Android-based applications that allow smart phones to read off fabric information from NFC tags were developed for this study using two methods. The system works as follows: 1. NFC tag stickers are attached onto the hangers where 200 fabric samples are hanging. 2. The NFC tag stickers are tagged, or read off from a smart phone that support NFC functions. 3. Upon tagging, the Smart phone swiftly displays all information available on its screen - not only the aforementioned six elements, but also the image of the clothing item from the fabric in its finalized product form, and the video of the model wearing the item - for convenient view. The method harbors immense potential for the fashion industry in general, and will also be useful in those fields inside the industry that harness NFC technology.

• Fisheries and Aquatic Sciences
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• v.5 no.3
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• pp.191-199
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• 2002

Pacific oysters Crassostrea gigas (Thunberg) were collected on April, 1999 and March­September, 2000 from Goseung Bay along the southern coast of Korea. The oysters tested cp;;ected from a depth of 0.5-2 m in which they cultured by a long line hanging method. The oxygen consumption rates (OCR) of oysters held under constant temperature and darkness (CC), were determined using an automatic intermittent-flow-respirometer (AIFR). Depending on holding periods after oyster collection, the experiments were divided into two groups: Group 7-d (held to ambient temperature for ca. 7 days) and Group 2l-d (held to ambient temperature for ca. 21 days). The OCR for Group 7-d single oyster displayed two peaks every day under CC, while Group 2l-d single oyster showed one peak every day. It is likely that the rhythmic patterns 02.6-12.8 hours) of the OCR in the Group 7-d single oyster may have been influenced by tidal currents at the sampling site. The rhythmic patterns (24.3-24.7 hours) in the Group 2l-d single oyster may have been shifted from two peaks to one peak each day under CC. The present study concludes that the OCR rhythm of wild oysters in nature is governed by two lunar-day clocks (24.8 hours); one driving one peak and the other driving the second peak. When oysters are subjected to the long-term CC conditions, one of the two-clock systems is depressed or only intermittently becomes active. Jpwever. the OCR rhythms by two to three oysters occurred arrhythmic patterns during the experiments and exhibited some evidence of weak rhythmicity of compared to those of a single oyster. It could be partly due to differences group effects.

• Journal of Korean Home Economics Education Association
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• v.4 no.1
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• pp.43-55
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• 1992

The purpose of this reasearch is to supply the basic data to improve Home Economics curriculum. This research was intended for 190 home economics teachers who are teaching in Kwang-Ju and chun-nam. The results are summarized as follows； 1. Among the objects of Home economics, the most important objects were to make students understand the significance of family life and development of family numbers. It requires 4 or 5 hours a week to learn these objects. 2. The amount of curriculum was immense and the level of curriculum was higher than students’level. More intencifying field are in this order； sex education, propriety education, consumption life, occupation, and computer education. 3. In home economics curriculum, the main stress was laid upon the application of action and learning content was focused on the matter set in the examination. 4. Theory and practical training classes were usually in the ratio of 7：3 and 8：2 in the current school classes. Ideal training classes are in the ration of 4：6, 5：5, 6：4, which showed that much weight was given to the practical exercises. 5. In practing subject matter, students tended to practice only main subject matter. The reason was primarily due to the lacks of equipments and faccilities, the limitation of training time for enterance examination and budgetary deficit. 6. Application of resorces was in the order of files, realia, samples, hanging chart, O.H.P and V.T.R. 7. The method of evaluation was mostly composed of paper and pens and practical evaluation when the practical evaluation was carrient out, in the case of necessity the standard of evaluation was made out.

• Earthquakes and Structures
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• v.18 no.4
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• pp.423-435
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• 2020

An accurate evaluation of structural damage is essential to performance-based seismic design for the structure across the earth fissure. By comparing the calculation results from three commonly used damage models and the experimental results, a weighted combination method using Chen model was selected in this paper as the seismic damage evaluation. A numerical model considering the soil-structure interaction (SSI) was proposed using ABAQUS software. The model was calibrated by comparing with the experimental results. The results from the analysis indicated that, for the structure across the earth fissure, the existence of earth fissure changed the damage distribution of the structural members. The damage of structural members in the hanging wall was greater than that in the foot wall. Besides, the earth fissure enlarged the damage degree of the structural members at the same location and changed the position of the weak story. Moreover, the damage degree of the structure across the earth fissure was greater than that of the structure without the earth fissure under the same excitation. It is expected that the results from this research would enhance the understanding of the performance-based seismic design for the structure across the earth fissure.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.6
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• pp.581-585
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• 2016

Nowadays medical treatment service increase due to ageing population and improving the quality of life. Accordingly it is predicted that drug discovery market will expansion continuously. Therefore it is necessary that profit creation by drug discovery on the global market is important. HCS(High Contents Screening) has been emerging as a potential method to solve problems of the present drug discovery. This research is about liquid dispenser for HCS. The purpose of this research is to manufacture high performance liquid dispenser for HCS. The dispenser is designed to control 5 liquids separately. The liquid contact parts are designed disposably, biocompatibly and chemically inertly. Air curtain is equipped at the side of tip to dispense very small liquid volume and to prevent hanging drop on tip. Valving is performed by just controlling the pressure of the pressure driven pump without valve.

• The Korean Journal of Pain
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• v.12 no.1
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• pp.114-118
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• 1999

Backgrouds: Cervical epidural blocks were used as part of a comprehensive multimodal treatment program for patients with chronic pain in the head, neck and upper extremities. The depth of the epidural space beneth the skin surface varies at different levels of the spinal column in the same patient. It also varies from patient to patient at the same vertebral level. We studied the distance the skin to the cervical epidural space in adults patients at different intervertebral spaces. Methods: Date were gathered from 628 patients having cervical epidural block for relief of cervical and upper extrimity pain. All blocks were performed using hanging drop method after loss of resistance with saline at C5-6, C6-7, C7-T1 intervertebral space. Results: Mean distances for skin to cervical epidural space (DSES) were 5.42 cm, 5.06 cm, 4.68 cm in male, 5.00 cm, 4.61 cm, 4.10 cm in female at C7-T1, C6-7, C5-6 intervertebral space. DSES correlated with body weight, neck circumference and body mass index significantly. Conclusion: In the cervical spine, DSES varies from space to space. The longest DSES were noted at C7-T1 level in male, and the shortest DSES were at C5-6 in female. DSES has significant relationships with weight, neck circumference and body mass index.

• The Korean Journal of Mycology
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• v.6 no.1
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• pp.21-27
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• 1978

The growing behavior of Candida albicans in various concentration of glucose and corticosteroid media was studied with the method of modified hanging slide culture. The strains of Candida albicans used in this study were obtained from vaginal swab from outpatients and were isolated from cultured colonies on Sabouraud's glucose agar media. To detect the budding rate of blastospore, the diluted suspension of Candida albicans in normal saline were inoculated into various concentration of glucose (Gl, G2, G3, G4), corticosteroid (S1, S2, S3, S4) and corticosteroid with 10% pepton broth (D1, D2, D3, D4) respectively and cultured at room temperature $(22{\sim}25^{\circ}C)$. The number of budding of blastospore were counted under the high power field of light microscope (400X) at specific time interval, e.g, 1, 2, 3, 6, 9, 12, 18, and 24 hours after inoculation. The results are as following: 1. The most effective budding rate was seen in G4 media (1.25% glucose) in 18 hours aft inoculation (89%). 2. The budding rate in Sabouraud's glucose broth with various concentration of dexamethasone added, was not significantly different from that of simple Sabouraud's glucose broth within 18 hours after inoculation, but there was statistically. significant difference in two budding rate at 24 hours observation. 3. The budding rate in 10% pepton broth media with various concentration of dexamethasone was almost same budding rate in control group, which is normal saline and 10% pepton broth, except on 2 and 24 hours results.