The purpose of this study was to clarify the effect of light emitting diode (LED) irradiation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Twenty-four male Sprague-Dawley rats were divided into four groups: excision (Ex), excision-LED irradiation (Ex-LED), diabetes + excision (DM) and diabetes + excision + LED irradiation (DM-LED). Diabetes was induced in rats by streptozotocin (STZ) injection (70 mg/kg, single dose) and 6 mm punch excision wounds were created on the back after shaving hair. The LED-irradiated rats were treated to a daily dose of $5\;J/cm^2$ LED (630 nm) light for 11 days after surgery, and were killed at day 1, 3, 7 and 11. The lesion and adjacent skin tissues were excised, fixed with 10% buffered formalin and embedded with paraffin. For evaluation of wound healing, hematoxylin-eosin (HE) and Masson trichrome staining were performed. Mast cells (MCs) were stained with toluidine blue (pH 0.5) and quantified using a computerized image analysis system. The proliferation activity of keratinocyte in skin tissues was analyzed on sections immunostained with proliferative cell nuclear antigen (PCNA). The results showed that wound healing rate, collagen density and neo-epidermis length, number of PCNA-positive cells, fibroblasts and mast cells were significantly higher in the LED-irradiated rats than in the DM and Ex rats throughout the periods of experiment. Exceptionally, the number of MCs was significantly lower at day 11 compared with day 7 after surgery in the all groups. These findings suggest that the LED irradiation may promote the tissue repair process by accelerating keratinocyte and fibroblast proliferation and collagen production in normal rats as well as in diabetic rats, and MCs may play an important role at an early stage of skin wound healing in normal and diabetic rats.
da Silva, Jamille Rodrigues;Maciel, Bianca Mendes;de Santana Souza Santos, Luana Karla Nogueira;Carvalho, Fabio Santos;de Santana Rocha, Daniele;Lopes, Carlos Wilson Gomes;Albuquerque, George Rego
Parasites, Hosts and Diseases
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제55권3호
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pp.239-246
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2017
Strains of Toxoplasma gondii in Brazil are highly genetically diverse compared to strains from North America and Europe. Dogs are epidemiologically important because they act as sentinels for T. gondii infections in humans and are good indicators of environmental contamination. The aim of this study was to isolate and genetically characterize T. gondii strains from tissues of naturally infected Brazilian dogs. For this study, 21 blood samples were collected from dogs at the Zoonosis Control Centers of $Ilh{\acute{e}}us$ and Itabuna cities, Bahia, Brazil. The sera were examined for T. gondii antibodies using the indirect hemagglutination test. Brains and hearts of seropositive dogs were bioassayed in mice to isolate and characterize T. gondii parasites by PCR-RFLP using 10 genetic markers (SAG1, newSAG2, SAG3, BTUB, c22-8, c29-2, GRA6, PK1, APICO, and L358). However, T. gondii was isolated from only 4 (57.1%) dogs, designated TgDgBr6, 13, 17, and 21. All strains were virulent, causing clinical changes (rough hair coat, lethargy, and abdominal distention) and the death of all mice within 8-20 days after inoculation. Genetic analysis of these 4 T. gondii isolates revealed 4 distinct genotypes with different clonal lineage combinations (types I, II, and III) and 2 atypical alleles. Using PCR-RFLP with several markers, this study contributes to evaluations of the genetic diversity of strains circulating in Brazil.
Objective: To explore the clinical efficacy and prognostic factors of chemoreduction combined with topical treatment of advanced intraocular retinoblastoma (RB). Materials and Methods: A total of 22 eyes from 17 children with RB were selected for the study and treated with chemoreduction combined with topical cryotherapy, transpupillary thermotherapy (TTT) or episcleral plaque brachytherapy. Clinical and follow-up data were retrospectively analyzed. Results: All children received 2~6 courses of chemoreduction treatment, ($4.5{\pm}0.8$ courses on average); 17 eyes from 13 children were treated by chemoreduction combined with cryotherapy or TTT and 5 eyes from 4 children with chemoreduction combined with $^{125}I$ episcleral plaque brachytherapy. The eye retention rate was 81.8% (18/22), among which 38.9% (7/18) featured restored or maintained good vision. Postoperative follow-up period was 7 to 34 months, ($18.6{\pm}5.2$ months on average). The recurrence rate was 41.2% (7/17), among which 57.1% (4/7) were controlled by supplementing or appending cryotherapy or TTT treatment during the follow-up period. The tumor basal diameter and thickness were significantly reduced (P<0.05 or P<0.01) after treatment. All children demonstrated different degrees of hair loss, 70.6% (12/17) with different degrees of gastrointestinal reactions, 5.88% (1/17) with neutropenia and 11.8% (2/17) being seriously infected during the chemotherapeutic treatment. Univariate and logistic regression analysis showed that tumor basal diameter before treatment had a significant effect on the prognosis (P<0.01). Conclusions: Chemoreduction combined with topical therapy can effectively control RB in the short term, and tumor basal diameter before treatment is an independent risk factor for prognosis.
Recently, the cosmetics industry considers male customers as a consumptive, active customer base who also have purchasing power with their stronger desire for their personal image enhancement. Male-preferred image brands have entered the era of unlimited competition through social media, thus increasing the acquisition of male beauty. Therefore, the information for designing favorable image strategies should be examined. This paper examines male awareness, determined by the degree of appearance management based on four categories (i.e., hair and skin care, makeup, foot and hand care, and plastic surgery). Based on the research, the paper proposes a data service to address the spatial and temporal separation problem between TV CF (the major media for recognizing information) and online $caf{\acute{e}}$/blog the major media for obtaining detailed information.
Sex pheromone composition was analyzed for Dichocrocis punctiferalis, which attacksapple, peach, chestnut fruits, etc., and its behavioral and field trapping studies were conducted to developa monitoring system with its sex pheromone. Virgin females showed maximum mating behavior andhairpencil extrusion behavior between 4-5 hrs after lights-off under 16L18D photoperiod and 26rf 1 "C.During this period, two sex pheromone components, ElO-hexadecenal and ZlO-hexadecenal, weredetected by GC analysis in the hexane extract of abdominal tips of virgin females. The best attraction ofmales to various synthetic sex pheromone blends was obtained at the 70 : 30 ratio of ElO-hexadecenal andZ10-hexadecenal for hair pencil extrusion and at the 80 : 20 ratio for flying upwind response. The highestattractiveness in fields was obtained between 70 : 30 and 80 : 20 from several tests in apple, peach andchestnut orchards.and chestnut orchards.
Journal of the Korean Society of Clothing and Textiles
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제30권2호
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pp.316-325
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2006
The colors of apparel are getting more important to give the differentiated character on fiber and fabrics. This study was to extract the favorable colors that become to facial color types. Research was carried out to classify the facial colors into several similar facial color groups. With JX-777, 2 points of face: forehead and cheek, were measured and classified into 3 facial color types. Sample size was 418 Korean adult males and other 15 of new males subjects. New chosen 3 subjects who had the classified facial color types, wore silver gown and black hat on his head to minimize the interaction of the clothe color an hair. The 40 standardized color samples were used to extract the favorable colors. 187 respondents answered the degree of becomingness of color samples on 3 facial color types. Data were analyzed by K-means cluster analysis, ANOVA and Duncan multiple range test using SPSS Win. 12. Findings were as follows: 1. 418 subjects who had YR colors were classified into 3 kinds of facial color groups. Type 1 was 4.59YR 5.89/5.12, Type 2 was 5.61 YR 5.41/4.79 and Type 3 was 4.38YR 6.49/4.89 respectively. 2. Favorable colors for Type 1 were 2 colors that belonged to ' a ' group from among colors that were divided into a, b, c group and 18 colors that belonged to ' a ' group from among colors that were divided into a, b group by Duncan post hoc test. 3. Type 2 showed that this type had many unfavorable colors. Unfavorable colors were 16 colors that belonged to ' c ' by Duncan test. 5. Favorable colors for Type 3 were 14 colors that belonged to ' a ' from among colors that were divided into a, b, c and 16 colors that belonged to ' a ' from among colors that were divided into a, b by Duncan test.
These studies were carried out to select the active grow hairy root lines induced from various ginseng(Panax ginseng C. A. Meyer) parts. Hairy roots were induced in root explants, stem and petiole in vitro by A. rhizogenes R1000 or A. rhizogenes $A_4$. These hairy roots could be grown on the phytohormone free medium, and PCR analysis of rol C and vir C gene fragments confirmed that hairy roots were transgenic tissues. We have selected 11 hairy root lines with active growing characters among 300 hairy root lines selected based on growth and morphological characteristics on 1/2MS solid media with 250 mg/L carbenicillin. Morphological characteristics of selected 11 hairy root lines were thickness and thiness of main roots, and many projection for lateral roots, active grow of lateral roots. Among selected 11 hair root lines prominent characteristics of hairy roots with active growing characters were thiness of main roots and active grow of lateral roots. But characteristics of low growing hairy roots were thickness of main roots and low grow of lateral roots. Finally we have selected actively growing hairy roots, KGHR-1, KGHR-5, KGHR-8 among 11 hairy root lines.
Objective: To explore the influence of serum vascular endothelial growth factor (VEGF) level on therapeutic outcome and diagnosis/prognostic value in patients with cervical cancer. Materials and Methods: A total of 37 patients diagnosed with cervical cancer by biopsy were selected and treated with concurrent chemoradiotherapy. Double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was adopted before treatment to assess VEGF levels, and its relationships with clinicopathological features and short-term therapeutic effects were analyzed. Results: The median VEGF level in 37 patients before treatment was 647.15 (393.35~1125.16) pg/mL. Serum VEGF levels in patients aged <50 years, in International Federation of Gynecology and Obstetrics (FIGO) stage IIIa~IVa, with lymph node metastasis and tumor size >4 cm were significantly increased (P<0.05). The complete remission (CR) rate was 48.7% (18/37), partial remission (PR) rate was 35.1% (13/37), stable disease (SD) rate was 13.5% (5/37) and progressive disease (PD) rate was 2.70% (1/37), so the objective remission rate (ORR) after treatment was 83.8% (31/37). Logistic regression analysis showed that tumor size and serum VEGF level before treatment were independent risk factors affecting the therapeutic outcome, and the higher the level of serum VEGF, the worse the prognosis when tumor size>4 cm. Some 56.8% of patients manifested with myelosuppression, 37.8% with leucopenia, 24.3% with thrombocytopenia, 5.41% with diarrhea, 46.0% with nausea and vomiting, 21.6% with hair loss and 8.11% with hepatic and renal injury during the treatment. Conclusions: Serum VEGF level may reflect the degree of malignancy of cervical cancer and predict therapeutic effect, which is of great importance to cancer diagnosis and prognosis.
Today, people are obsessed with fashion, makeup, hair care, weight control, and plastic surgery. In order to be satisfied with their appearance and attract attention, they invest so much time and money. The core values of many people are influenced heavily by materialism and appearance. Belly dancing is effective not only in just weight loss, but it also improves the health and corrects body postures like pelvis correction, and it also gives the dancers the pleasure of dancing to exotic music. A growing number of women in their 40s or older and senior citizens have become maniacs of belly dancing. The studies suggest that the participation in belly dancing helps increase life satisfaction and self-respect of the middle aged who often suffer from depression. Belly dancing has a strong effect in the life style of the middle-aged women. The costume and accessories necessary for belly dancing class help the participants) focus on the dance. These costumes and accessories reflect the desire of the wearer to be recognized in society. It is well shown in belly dancing that "fashion and beauty related services and products are used as an indicator to tell social status and position of the wearer as external elements like clothes, accessories, and physical appearance and have a highly important effect on interactions among people in society as a means of expressing oneself". In this respect, this study aims to examine middle-aged women's involvement in fashion and beauty depending on their participation in belly dancing.
Importance of appearance as a means of self-expression and communication is increasing in modern society even for men. And appearance has become an significant factor affecting men's quality of life. The main objectives of this study were to develop an instrument measuring appearance related quality of life(ARQoL), and examine the body factors influencing ARQoL. The ARQoL instrument was developed from in-depth interviews, focus group interviews, and literature review, and were revised through two pre-tests. The final questionnaire was administered through on-line survey with 440 men. Quota sampling was used based on age and body size. The results indicated that high ARQoL group showed significantly higher satisfaction on body factors including face, weight, shape, body proportion, hair, and overall impression than low ARQoL group. There was no significant difference in terms of satisfaction on height between two groups. Factor analysis showed that ARQoL consists of four factors - physical, economic, psychological and social factors. Overall impression was the most important body factor affecting all four factors of ARQoL and has very strong influence especially on economic ARQoL. BMI was also a significant factor affecting physical, economic and social ARQoL except psychological factor of ARQoL. Face had significant influence only on economic and psychological aspects of ARQoL.
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