• 한방안이비인후피부과학회지
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• 제18권3호
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• pp.66-74
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• 2005

Objective: Stress is one of the most important etiological factors in our lives. Human body makes effort to keep his homeostasis from stress by using autonomic nerve system. So we need markers which can evaluate autonomic nerve system easily. HRV represents such markers. Sympathetic and Parasympathetic nerve system are part of autonomic nerve system, related with cur body and diseases in ENT, Dermatology, Ophthalmology are also deeply related with stress, Sympathetic and Parasympathetic nerve system. So we can expect that it can help understanding patients to evaluate autonomic nerve system with HRV Research Methods and Procedures: Forty one patients who have seif-recognized stress in Dept. of Oph & Otolaryngo & Dermatology were selected. SDNN. RMSSD, LF, HF, norm-LF, norm-HF, LF/HF ratio, LnLF, LnHF were checked. Results: Men showed lower SDNN, HF, and LnHF than women(p<0.05). Age over-forty group showed significant lower SDNN, LF, HF, LnLF, LnH(p<0.01), and lower RMSSD(p<0.05) than age under-forty group. There's no big differences for age at norm-LF, norm-HF, and LF/HF ratio. Skin disease group showed higher SDNN, RMSSD, LF, HF, LnLF, LNHF than the others, facial palsy group showed higher norm-HF. LF/HF ratio, and mouth-throat group showed higher norm-LF, but there's no statically significant difference. Comparing skin disease group with the others, the others showed statically significant low SDNN(P<0.01), RMSSD, LF, HF, LnLF and LnHF(P<0.05).

• 드라이브 ㆍ 컨트롤
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• 제20권2호
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• pp.31-39
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• 2023

The aim of this study was to measure and analyze engine load factor (LF) according to working conditions (operation type and gear stage) of small agricultural multi-purpose cultivator to estimate the emission of air pollutants. To calculate LF, a torque sensor capable of collecting torque and rotational speed was installed on the engine output shaft and DAQ was used to collect data. A field test was conducted with major operation of a cultivator and tillage operations (plow tillage and rotary tillage). Engine power was calculated using engine torque and rotational speed and LF was calculated using real-time power and rated power. In addition, unified LF was calculated using the weight for each operation and the average LF for each operation. As a result, average LF values at 1.87 and 3.10 km/h by plow tillage were 0.50 and 0.69, respectively. Average LF values at 1.87 and 3.10 km/h by rotary tillage were 0.70 and 0.78, respectively. Furthermore, unified LF calculated in consideration of the weight factor showed a value of 0.65, which was 135% higher than the conventional LF (0.48). Results of this study could be used as basic information for realizing LF values in the field of agricultural machinery.

• Food Science and Biotechnology
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• 제14권1호
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• pp.131-136
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• 2005

The selective extraction behavior of lactoferrin (Lf) from whey protein mixture was examined using reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide (CDAB). The major whey proteins, including ${\beta}$-lactoglobulin, ${\alpha}$-lactalbumin and bovine serum albumin, were solubilized from aqueous phase to organic phase while Lf was recovered in the aqueous phase. The solubilization behaviors of the proteins were manipulated by the process parameters such as the pH and salt concentration of the aqueous phase and the surfactant concentration in the organic phase. Efficient forward extraction was achieved with sodium borate buffer (50 mM, pH 9) containing 50 mM KCl and organic phase containing 100 mM CDAB. Based on SDS-PAGE and densitometry, about 96% of the initial Lf remained in the aqueous phase after forward extraction. The dialyzed Lf fully maintained its bacteriostatic activity against E. coli O157:H7.

• 유기물자원화
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• 제28권1호
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• pp.27-36
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• 2020

본 연구는 친환경 토마토(Lycopersicon esculentum var. cerasiforme) 농가에서 대표적으로 자가 제조되고 있는 액비를 노지에 관주하였을 때 생장과 수량에 어떠한 영향을 미치는지를 구명하기 위하여 수행되었다. 처리는 무처리(물), 유박, 골분+어분, 생선아미노산, 깻묵, 불가사리 액비를 포함하였다. 깻묵과 불가사리 액비의 시기별 pH는 보관 30일 이후에 크게 감소하였고 두 자재의 EC는 0.6~0.8 dS/m으로 가장 높았고 골분+어분액비도 0.4 dS/m 전후로 높은 수준이었다. 액비의 전질소 농도는 불가사리 액비가 0.0062%로 가장 높았고 깻묵(0.0059%) > 골분+어분(0.0030%)순이었고 칼륨도 위의 세 가지 액비에서 가장 높았다. 액비의 인산과 칼슘은 생선아미노산 자재에서 가장 높았고 마그네슘은 식물성인 깻묵 자재에서 유의성 있게 높게 나타났다. 토양 EC는 불가사리 액비 처리구에서 0.74 dS/m로 가장 높았지만 토양과 잎의 대량 무기성분 농도는 처리 간에 차이가 관찰되지 않았다. 잎의 건물중과 엽온 및 줄기의 직경과 초장을 15일에 한 번씩 조사한 결과, 잎 건물중은 시기별로 무처리구에서 대부분 낮았고 초장도 생육 초기에 무처리구에서 가장 작았지만 후기에는 자가제조 액비와 비슷한 수준이었다. 과실수량과 평균과중은 무처리구에서 처리 후 75일 까지 유의성 있게 낮았다. 과실횡경은 골분+어분과 불가사리 액비 처리로 가장 증가하였고 과실 당도와 착색은 생선아미노산 액비구에서 가장 향상되었다. 과실경도는 골분+어분 처리에서 가장 높았다.

• Food Science and Biotechnology
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• 제16권5호
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• pp.710-714
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• 2007

Ligularia fischeri (LF) has been used to treat jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases. This study was carried out to determine the antioxidant activity of the extract from the leaves of LF by using various established in vitro systems with ${\alpha}$-tocopherol as positive control. The results showed that the ethanol extract of LF (0.05-0.5 mg/mL) displayed a strong free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl, ${O_2}^{{\cdot}-}$, and $H_2O_2$ radicals. It was observed that LF extract (1 mg/mL) significantly decreased the levels of malondialdehyde (MDA) to 69 and 89% of control, measured by ferric thiocyanate (FTC) and thiobarbituric acid (TBA) test, respectively. LF extract (0.5-2 mg/mL) was also found to inhibit significantly the amount of malondialdehyde formed from liver homogenate to 69-56% of control. Similarly, in the high $Fe^{2+}/ascorbate$ induction system LF extract (1-2 mg/mL) inhibited carbonyl formation measured by 2,4-dinitrophenylhydrazine reaction to 89-79% of control. Like antioxidant activity, the reducing power of LF extract was excellent at concentration of 0.5-2 mg/mL. These results indicate that LF could be used as a potential source of natural antioxidant.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.224-229
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• 2009

Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.

• 전자통신동향분석
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• 제31권3호
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• pp.50-59
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• 2016

Light-field(LF) 기술은 자연스러운 완전 입체영상을 재현하면서도 홀로그램에 앞서 실현 가능한 기술로 알려져 있으며, 이에 대한 기술개발이 국내외에서 활발히 진행되고 있다. LF 기술은 카메라로 빛의 세기와 방향 정보를 획득 후 디스플레이로 그대로 재현함으로써 시각적으로 왜곡이 없는 완전 입체의 실감영상을 시청하게 하는 것이다. 조밀한 시점영상, 깊이영상의 제공으로 3D 단말 분야뿐만 아니라, 초점조절 기능을 통해 다양한 2D 응용 기술로 활용할 수 있다. LF 기술은 대형 디스플레이에서 완전 입체영상 제공을 목적으로 개발이 시작되었지만, 최근에는 모바일 단말, HMD와 같은 개인용 단말에 LF 기술을 적용하기 위한 기술개발이 시도되고 있다. 본고에서는 LF 카메라 및 단말 기술의 원리와 개발동향에 대해 살펴보고자 한다.

• Journal of Microbiology and Biotechnology
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• 제13권3호
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• pp.338-343
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• 2003

Various factors, such as the adsorption pH, adjuvant dose, and adjuvant age, which affect the adsorption degree and immunogenicity of an antigen, were investigated. In addition, the effect of pH, antigen content, and adjuvant content on immunogenicity was also studied through animal experiments. Within the ranges studied, a low pH for adsorption, freshly preformed gel, and low pH formulation for the combined DTwP-HepB vaccine were preferrable for the adsorption of the antigens. In addition, a higher DT content was found to have a positive effect on the HBsAg immunogenicity in the combined vaccine. Accordingly, considering the factors affecting the adsorption rate and immunogenicity of the antigens, a novel DTwP-HepB vaccine (40 Lf/ml of diphtheria toxoid, 15 Lf/ml of tetanus toxoid, 20 OU/ml of detoxified whole cell pertussis, $24\;\mu\textrm{g}$ of HBsAg, $24\;\mu\textrm{g}\;Al/ml\;of \;Al(OH)_3\;gel,\;776\;\mu\textrm{g}\; Al/ml\;of\;AIPO_4\;gel$, and pH 7.1) was developed, whose immunogenicity was comparable to the case of administrating, separately and simultaneously, a combined DTwP vaccine (40 Lf/ml of diphtheria toxoid, 15 Lf/ml of tetanus toxoid, 20 OU/ml of detoxified whole cell pertussis, $300\;\mu\textrm{g}\;Al/ml\;of\; AIPO_4\;gel$, and pH 7.1) and mono HepB vaccine [$Hepavax^{\circledR},\;24\;\mu\textrm{g}/ml$ of HBsAg and $500\;\mu\textrm{g}\;Al/ml\;of\;Al(OH)_3\;gel$], which satisfies the potency criteria of the K-FDA for a combined DTwP vaccine and mono HepB vaccine.

• 생약학회지
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• 제45권1호
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• pp.88-92
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• 2014

Lysimachia foenum-graecum (LF) has been used for home remedy of common cold and headache in China. Foenumoside B from LF has been reported for anti-obesity effects. We used foenumoside B as a marker for content evaluation of different parts of LF. Ethanol extract of LF was used for isolation of foenumoside B and purified further by column chromatography. The structure was identified as foenumoside B by interpretation of spectroscopic analysis, including $^1H$-, $^{13}C$-NMR and FT-ICR-MS. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of foenumoside B in different parts of LF. Foenumoside B contents in the leaf, stem, and aerial part showed significant differences. Contents of foenumoside B was detected highly in the leaf extracts. The results would be useful for efficient extraction of foenumoside B in LF.

• 대한본초학회지
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• 제30권6호
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• pp.47-53
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• 2015

Objectives : This study was performed to investigate the anti-diabetic effect of the fruit extract of Lycium chinense Mill(Lycii Fructus, LF) on multiple low-dose streptozotocin-induced diabetic rats.Methods : Male Sprague-Dawley rats were divided into three groups; normal, STZ-control, Lycii Fructus extractorally administrated 300 ㎎/㎏ group (STZ-LF). Diabetes was induced in rats by consecutive injection of streptozotocin (STZ) at doses of 30 ㎎/㎏ for 5 days. After 4 weeks, all rats were sacrificed, Fasting blood glucose, total cholesterol (CHO), triglyceride (TG) and HDL-Cholesterol were measured in sera of rats. Histopathological changes of pancreas, kidney, liver and lung tissues were observed by microscope after H&E, Periodic acid-Schiff (PAS) and Masson's trichrome staining. The changes of body weight, blood glucose, and food and water intake were also measured.Results : There were no differences in body, food intake and water intake in LF-administrated groups compared with STZ control group. However, LF extract significantly decreased the levels of serum glucose, CHO, TG and HDL-Cholesterol in diabetic rats. In histopathological analysis of kidney, liver and lung, LF-administrated groups showed the inhibition of morphological damage.Conclusions : These results suggest that LF have a biological action on multi low-dose STZ-induced diabetes in rats via decreasing the serum glucose, TG and TG levels and may protect the morphological changes of kidney, liver and lung.